투과전자현미경 내 직접 가열 실험에서의 실험적 문제들

김영민,김진규,김양수,오상호,김윤중 韓國電子顯微鏡學會 2008 Applied microscopy Vol.38 No.4

In performing in situ heating transmission electron microscopy (TEM) for materials characterizations, arising concerns such as specimen drifts and unintentional Cu contamination are discussed. In particular, we analysed the thermal and mechanical characteristics of in situ heating holders to estimate thermal drift phenomena. From the experimental results, we suggest an empirical model to describe the thermal drift behavior so that we can design an effective plan for in situ heating experiment. Practical approaches to minimize several hindrances arisen from the experiment are proposed. We believe that our experimental recommendations will be useful for a microscopist fascinated with the powerful potential of in situ heating TEM.

Overexpression of Zoysia ZjCIGR1 gene confers cold stress resistance to zoysiagrass

Yang‑Ji Kim,Dae‑Hwa Yang,Mi‑Young Park,Hyeon‑Jin Sun,Pill‑Soon Song,Hong‑Gyu Kang,Seok‑Cheol Suh,Yong‑Eok Lee,이효연 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.1

Zoysia japonica Steud. is a warm-season lawn grass popular in Korea and elsewhere. They are cultivated in many places such as river banks, roadside, and play grounds. However, there still is a disadvantage of frequent mowing, and the grass grows poorly under the chilly conditions. To develop a grass variety that circumvents these drawbacks, we cloned the chitininducible gibberellins-responsive1 gene (CIGR1) from zoysiagrass. The full length of the ZjCIGR1 (Zj; Zoysia japonica Steud.) gene was obtained by 5′/3′ RACE PCR and the phylogenetic tree showed that it belonged to the CIGR1-subgroup in the PAT1-group of GRAS protein family. Expression of the ZjCIGR1 in wild-type plants was confirmed in roots, meristems, leaves, and flowers, especially high in the flowers. The transgenic zoysiagrass was confirmed by PCR using gene-specific primers, phosphinothricin-acetyl-transferase (PAT) strip test, and Southern blot analysis. ZjCIGR1-overexpressing plants acquired tolerance to cold stress displaying morphological phenotypes characteristic of stress resistance. In addition, in the transformants, expression of the ZjCIGR1 as well as cold-regulated (COR) gene was increased compared to the wild-type plants under cold stress condition. These results suggest that ZjCIGR1 gene is an important candidate for regulating cold stress resistance.

전자 빔 조사 민감 물질의 전자회절분석을 위한 Imaging Plate 기술

김영민,김양수,김진규,이정용,김윤중 韓國電子顯微鏡學會 2008 Applied microscopy Vol.38 No.3

전자 빔 조사 민감 물질인 gibbsite (γ-Al(OH)₃)의 전자 빔 조사 상전이 연구에서 전자회절 자료의 기록에 대한 imaging plate 와 필름의 기록 특성을 실험적으로 비교하였다. Imaging plate는 극단적으로 낮은 전자 강도와 높은 전자 강도를 동시 기록하기에 충분한 선형 dynamic range를 갖기 때문에, 매우 낮은 전자조사 조건 (≤0.1 e-/μm²)에서 전자 회절 자료를 기록할 때 필름에 비해 회절 자료의 spatial frequency 범위가 두 배 이상 확장되었다. 심지어 이미 기록 포화된 투과 빔 주위의 신호 정보 레벨을 세분화하는 데에도 훨씬 우수한 분해 성능을 나타내었다. 따라서 본 연구 결과는 imaging plate가 극단적으로 낮은 전자강도 기록이 필요한 전자 빔 조사 민감 물질이나 cryo-biological 시편들의 구조 연구 관점에서 가장 적절한 기록 매체임을 나타낸다. An experimental comparison of the detection properties between imaging plate and film for recording the electron diffraction pattern was carried out on a radiation-sensitive material, an aluminum trihydroxide (gibbsite, γ-Al(OH)₃), through the electron beam irradiation. Because the imaging plate has a wide dynamic range sufficient for recording extremely low- and high-electron intensities, the range of spatial frequency for the diffraction pattern acquired by the imaging plate was extended to two times larger than the range by the film, especially at a low electron dose condition (≤0.1 e-/μm²). It is also demonstrated that the imaging plate showed better resolving power for discriminating fine intensity levels even in saturated transmitted beam. Hence, in the respect of investigating the structures of radiation-sensitive materials and cryobiological specimens, our experimental demonstrations suggest that the imaging plate technique may be a good choice for those studies, which have to use an extremely low electron intensity for recording.

3-Joint Planar Type 로봇 매니퓰레이터의 자기동조적응제어에 관한 연구

김규로,양희주,유용석,최명진,오택열 慶熙大學校 大學院 院友會 1996 高凰論集 Vol.17 No.-

This study has parallelogram trajectory tracking of Three Joint Planar type Robot Manipulator. The equation of motion for the three joint planar manipulator are obtained by substituting Lagrange's energy expression into Euler-Lagrange's equation and forming the appropriate derivative. Lagrangian method allows us to obtain the dynamics equations for very complicated systems in the simplest manner possible. SISO model is used for design of the controller. The design of controllers may be considered primary controller for manipulators to track the desired trajectory under ideal conditions and secondary controller to compensates for undesirable deviations of the motion from the trajectory caused by external and internal disturbances. The aim of this paper is to evaluate the efficiency by self-tunning adaptive control and considering a characteristic of control. Through the computer simulation, obtained the following results 1) selt-tunning adaptive control is superior to PD control, 2) During the learning period, the adaptive control algorithm is searching proper estimates and controller gains. After this period, the outputs of the joints start tracking closely the desired valued.

二面交雜에 依한 고추 開化所要日數의 遺傭分析

金?椿,朴圭煥 COLLEGE OF AGRICULTURE KYUNGPOOK NATIONAL UNIVERSI 1984 慶北大農學誌 Vol.2 No.-

This study was performed to obtain the basic informations on days to first flower in red pepper. Eight parent varieties and twenty eight F₁ hybrids from 8X8 half diallel cross were used as the materials, and heterosis, combining ability, the degree and direction of dominance, and heritability were evaluated. The 1% significances in day to first flower were observed among parents and F₁s, and the mean of F₁s was not significantly longer than parents. Positive and negative heterosis and heterobeltiosis were observed. Both average heterosis and heterobeltiosis were negative. General combining ability (GCA) and specific combining ability (SCA) variances were highly significant, and GCA variances were greater than SCA variances. Partial dominance was observed in days to first flower and the direction of dominance was negative excluding Chunan and Saegochu varieties. The number of effective gene was estimated as one, and heritabilities in narrow and broad sense were fairly high.

水 海洋係 專門大學 通信科 敎育課程 開發 硏究

金永煜,金秀植,高南泳,梁義藏,金圭煥,文建,鄭東洙 군산대학교 1984 群山水産專門大學 硏究報告 Vol.18 No.1

We have studied on the curriculum to develop the communication department of the inter-related fisheries and marine junior college groups and abridged them as follows. (1) A vessel communication has the special characteristics compared with general communication field and also it has an aim to protect the lives and the property from dangerous navigation. For these reasons we must educate many competent graduates who are versed in business and accustom themselves to necessary knowledge of communication and can be adapted them selves to the advanced communication method, techniques and international information on the communication. (2) Training goal and way of thinking of students were rearranged and the occupational cluster in the electronic-communication field was fractionated to develop the curriculum. Especially regarding operator's duty served the above purpose as a occupational cluster, we analyzed their job in accordance with it. (3) International convention on standards of training certification and watchkeeping for seafarers, 1978 was reflected, also international telecommunication convention was referred so that it might be satisfied with the condition to be observed. (4) Developing the curriculum, we responed and analyzed the questions from scientific world, industrial organizations and graduates and provide their advice and suggestions form consultive committee and reflected them to develop the curriculum

MRAC를 이용한 로보트 매니퓰레이터의 制御性能 向上에 관한 硏究 : Model Reference Adaptive Controller

김병수,김규로,선양래 慶熙大學校 大學院 1995 高凰論集 Vol.16 No.-

This paper is study on trajectory property of 6 DOF robot manipulator. This paper used to adaptive control and p-d(proportional-derivated) control for control methods, Simulation robot is stanford 6-dof robot manipulatr of stanford university. Main results obtained through this paper are summarized as followings. At each links, adaptive control has more excellant control than p-d control. At 1-st link, adaptive control and p-d control has a little error at 3.0 second. At 2 nd link, adaptive control and p-d control has a little error than other links without 3-rd link, At 3-rd link, two control methods has a superiority control results than other links with two control methods. At 5-st link, p-d control error is a most great than other links. Therefore, the adaptive control applications for 6-dof robot manipulator has a very excellant capability than applying classical p-d control.

專門大學 電子通信 技術人力을 위한 産學協同敎育方案 硏究

金圭煥,高南泳,梁義藏 군산대학교 1987 群山水産專門大學 硏究報告 Vol.21 No.1

In modern industry, the reorganizations of organization and job classification of telecommunication are required for the Advanced Information Society (AIS) which is being accomplished by the entrance of new media, Information Service Noetwork plan for Korean government offices (ISN) and Integrated Services Digital Network (ISDN). Accordingly, the demand of competent technician would be increased for performance of the related job. In this paper, educational system of College-Industry-Korea Vocational Training and Management Agency Cooperation for the telecommunication department was studied for the effective education of the specified technician to be engaged in the specified job. The results were as follows. 1. By the establishment of College-Industry-Korean Voctional Training and Management Agency and their linking operation, the gap in their technical level could be narrowed. In addition, the effective improvement for the field practice, the official approval system, the normalization of the special education and the settlement of its related cooperation business with administrative agencies could be advanced promptly. 2. The industry should take the advancement of its technical level by establishing of employ job for the professional course technician of junior college graduates and by hiring of the 2nd class licensed technician for the related job. 3. Telecommunication department should carried out not only the efficient operation of curriculum (Table 13.) and development of teaching materials fur special technical education according to each course , but also the substantiality of field practice. 4. Korea Vocational Training and Management Agency is desired to develope the problems of examination approached to practical technique level of industry and to improve the applicant qualification, examination date and method of practical examination for normalization of technical education in junior college telecommunication department.

NOD/SCID 마우스 모델을 이용한 인간 제대혈 혼합이식에서의 생착 양상 분석

정양조,김동욱,조빈,강영주,박보배,김혜정,김태규,오일환 대한조혈모세포이식학회 2002 대한조혈모세포이식학회지 Vol.7 No.2

연구배경: 제대혈은 골수에 비해 많은 장점을 가지고 있음에도, 이식 후 성적에 있어 총세포수에 의해 좌우되며, 한 개의 태반으로부터 얻을 수 있는 총세포수의 한계가 있어 주로 소아에 국한되고 있다. 따라서 제대혈 응용의 확대를 위해 생착량을 늘이기 위한 방법으로 다중공여자에 의한 제대혈 혼합이식을 늘일 수 있는지 연구하기 위하여, 본 연구에서는 주조직적합성 형별로 조합된 제대혈을 NOD/ SCID 생쥐에 이식하는 방법으로, 혼합생착의 가능성을 연구하였다. 방법: 조합된 2개 공여자로부터 얻어진 제대혈들을 단일이식 및 혼합이식하여, 혼합이식된 숙주에 생착된 세포를 PCR-SSOP 방법을 사용하여 공여자 별로 추적하였다. 또한 각 공여자세포의 상대적 생착비율을 대조군인 단일제대혈 이식에서 얻어진 생착량과 비교하는 정량적 비교도 함께 하였다. 결과: 총단핵구를 혼합이식한 실험군에서는 주조직적합성의 일치도에 관계없이 한쪽공여자의 세포가 다른쪽에 비해 우세한 편향생착을 보였다. 그러나 임파구를 비롯한 Lineage 양성세포를 제거한 실험군에서는 주조직적합성의 불일치에도 불구하고 뚜렸한 생착의 공존이 관찰되었다. 결론: 본 연구는 제대혈의 생착량을 증가를 목적으로 총단핵구를 혼합이식할 경우 한쪽공여자의 세포에 의해 편중되며, 이것을 이식 후 생착과정에서의 두 종류의 세포의 경쟁에 의해 초래된다는 것을 보이고 있다. 또한 이러한 경쟁은 제대혈에 포함되어 있던 임파구들에 의한 것이며, 생착공존을 위하여는 공여자간 주조직적합성의 일치도 보다, 임파구의 제거가 더 중요하다는 것을 보여주고 있다. Background: Although umbilical cord blood (UCB) has been an attractive alternatives for bone marrow cells, application is limited to pediatric case due to limitation in total number of nucleated cells, that is a single most important factor for outcomes of UCB transplantation. Therefore, increasing overall engraftment by mixed transplantation of UCB derived from multiple donor should comprise one strategy to circumvent the posed limitation of UCB transplantation. In order to investigate the feasibility of establishing co-engraftment by multi-donor UCB cells, we carried out a xenotransaplantation study using NOD/SCID mice for systemic analysis of results on the input-based control of single unit transplantation. Methods: UCB units with various extent of HLA-matchings were co-transplanted into NOD/SCID mice along with single unit transplantation control. The relative contributions of engraftment by cells from each donor-derived were analyzed by HLA polymorphism using PCR-SSOP. Results: In all HLA-based mixed transplantation of total nucleated UCB cells, engraftment of one donor predominated over the other despite that equivalent amount of engraftments were achieved by single donor transplantation. When lineage depleted UCB units were co-transplanted, significant degree of co-engraftments were observed regardless of HLA disparity. Conclusion: Our result show that one donor cells dominate over the other in mixed UCB transplantation and that it is due to competition between donor cells during post-transplantation process. Our results suggest that immune cells contained in UCB unit mediate such competitioin and that 6 locus HLA matching would not be sufficient to prevent the competition.

Java 병행프로그래밍 도구의 개발

박양수,김현규,문남두,이명준 울산대학교 1999 공학연구논문집 Vol.30 No.2

분산응용시스템은 서비스의 효율향상을 위해 일반적으로 병행성을 지원하는 것이 바람직하다. 그러나 병행성을 지원하기 위한 프로그래밍 기법은 난이도가 높은 구현 기법이며, 대부분의 프로그래밍 언어에서 이를 직접적으로 지원하지 않으므로, 분산응용시스템을 위한 개발자의 부담을 가중시키고 있다. 본 논문에서는 현재 분산응용시스템의 작성에 널리 쓰이고 있는 Java에서 병행성을 체계적으로 지원하기 위한 기반으로서, 세미포어나 조건변수와 같은 고수준 동기화 도구의 개발에 대해 소개하였다. 이러한 Java고수준 동기화 도구는 순수한 Java로 개발되어 이식성을 지니고 있으며, 개발자가 쉽게 기술할 수 있는 인터페이스를 제공하고 있다. 또한 Java가 지닌 대중성으로 인해 대부분의 분산응용시스템의 구축에 널리 사용될 수 있을 것으로 기대된다. In a development of distributed applications, it is desirable for a server to support concurrency in order to provide effective services. However, the programming mechanism for concurrency is difficult to implement correctly, so most programming languages do not support this facility directly. Therefore, the fact imposes extra burdens to the developers for distributed applications. The high-level synchronization tools such as Semaphores and Condition Variables in Java are suggested as a basis for systematically supporting concurrency control. The suggested high-level synchronization tools also have a good portability since it has developed in pure Java. In addition, due to the popularity of Java, these tools will ho a long way with developers in building distributed systems.