Analysis of genetic diversity among indigenous landraces from sesame (Sesamum indicum L.) core collection in China as revealed by SRAP and SSR markers

Yan‐xin Zhang,Wei Hua,Lin‐han Wang,Zhuo Che,Xiu‐rong Zhang 한국유전학회 2010 Genes & Genomics Vol.32 No.3

The molecular genetic diversity of 404 indigenous landraces from sesame core collection in China were evaluated by 11SRAP and 3 SSR markers, 175 fragments were generated, of which 126 were polymorphic with an average polymorphism rate of 72%. Jaccard’s genetic similarity coefficients (GS=0.7130), Nei's gene diversity (h=0.2418) and Shannon's Information index (I=0.3847) were calculated, a dendrogram of the 404 landraces was made, landraces from various zones were distributed throughout the dendrogram, accessions from different agro‐ecological zones were indistinguishable by cluster analysis, geographical separation did not generally result in greater genetic distance, a similar pattern was obtained using principal coordinates (PCO) analysis. As to seven agro‐ecological zones, the maximum Nei’s gene diversity (h = 0.2613)and Shannon index (I = 0.3980) values in zone VII indicated that they were genetically more diverse than those in other zones, while the least genetically diverse region was zone III (h = 0.1772, I = 0.2858). Nei's genetic identity and genetic distance among landraces from seven agro‐ecological zones were also analyzed, the genetic relationship of seven zones was inferred using the UPGMA method. This study demonstrated that SRAP and SSR markers were appropriate for evaluation of sesame genetic diversities. There existed extensive genetic diverse among indigenous landraces and the abundance of genetic diversity of landraces in different agro‐ecological zones was various. Understanding of these characteristics of indigenous landraces in China can provide theoretical foundation for further collection, effective protection and reasonable utilization of these sesame landraces in breeding.

Implementation of online model updating with ANN method in substructure pseudo-dynamic hybrid simulation

Yan Hua Wang,Jing Lv,Yan Feng,Bo Wen Dai,Cheng Wang,Jing Wu,Zi Yan Chen 국제구조공학회 2021 Smart Structures and Systems, An International Jou Vol.28 No.2

Substructure pseudo-dynamic hybrid simulation (SPDHS) is an advanced structural seismic testing method which combines physical experiment and numerical simulation. Generally, the key components which display nonlinearity first are taken as experimental substructures for actual test, and the remaining parts are modeled in simulation. Model updating techniques can be effectively applied to enhance the model precision of nonlinear numerical elements. Specifically, the constitutive model of the experimental substructure is identified online by the instantaneously-measured data, and the corresponding numerical elements with similar hysteretic behaviors are updated synchronously. Artificial neural network (ANN) can recognize the system which cannot be represented by definite numerical model, and thus avoids the structural response distortion caused by the inherent numerical model defects. In this study, a framework for online model updating in SPDHS with ANN method is expanded to implement actual test validation. Moreover, the effectiveness of ANN method is demonstrated by practical tests of a two-story frame model with bending dampers. Additionally, the unscented Kalman filter technique and offline ANN identification approach are both examined in the test validation. The experimental results show that, under the identical loading history, the online ANN method can significantly reduce the model errors and improve the accuracy of SPDHS.

EXPERIMENTAL STUDIES ON SPONGE ALUMINUM ALLOY BY SUCTION CASTING PROCESS

Hua, Hua, Xiang,Wu, Wu, Binyao,Yan, Yan, Xiaolong 한국재료학회 1995 Fabrication and Characterization of Advanced Mater Vol.1 No.2

Spomge metal with 3-dimensionally interconnected fine pores is a kind of functional material used in the areas of shock attenuation, noise elimination, filtration, decoration, fire-proof, etc. Currently most of them are produced by penetrating casting process, limited shaping size due to its penetrating resistance and difficulties in controlling it's complicated procedure are two main problems. Using soulble granules as fillings, applying the suction casting method with self-made devices, the effect of preheating temperature of granules, pouring temperature metal and vacuum on the shaping size, porosity and the uniformity of pores at every distinct filling sizes are systematically investigated. Experimental results show tha the preheating temperature of granules is the key fat, while the e pouring temperature of metal and negative pressure are the second at a fixed grain size ; the nearer the preheating temperature of fillings to pouring temperature of the metal, the better the suction casting moulding. For the eurectiv Al-Si alloy, a speong-like cylinder, with the porosity of 70-80%, 200mm in height and 120mm in diameter, can be formed when the temperature of liquid metal gets 750 centigrade and preheating temperature reaches 420 centigrade. The Minimum size of the proes reaches ${\phi}0.05mm$.

A Novel Role of Classical Swine Fever Virus E(rns) Glycoprotein in Counteracting the Newcastle Disease Virus (NDV)-mediated IFN-β Induction

( Yan Hua Xia ),( Liu Chen ),( Zi Shu Pan ),( Chu Yu Zhang ) 생화학분자생물학회 2007 BMB Reports Vol.40 No.5

Associations of Plasma Glucagon Levels with Estimated Glomerular Filtration Rate, Albuminuria and Diabetic Kidney Disease in Patients with Type 2 Diabetes Mellitus

Hua-Xing Huang,Liang-Lan Shen,Hai-Yan Huang,Li-Hua Zhao,Feng Xu,Dong-Mei Zhang,Xiu-Lin Zhang,Tong Chen,Xue-Qin Wang,Yan Xie,Jian-Bin Su 대한당뇨병학회 2021 Diabetes and Metabolism Journal Vol.45 No.6

Background: Type 2 diabetes mellitus (T2DM) is characterized by elevated fasting glucagon and impaired suppression of postprandial glucagon secretion, which may participate in diabetic complications. Therefore, we investigated the associations of plasma glucagon with estimated glomerular filtration rate (eGFR), albuminuria and diabetic kidney disease (DKD) in T2DM patients.Methods: Fasting glucagon and postchallenge glucagon (assessed by area under the glucagon curve [AUCgla]) levels were determined during oral glucose tolerance tests. Patients with an eGFR <60 mL/min/1.73 m2 and/or a urinary albumin-to-creatinine ratio (UACR) ≥30 mg/g who presented with diabetic retinopathy were identified as having DKD.Results: Of the 2,436 recruited patients, fasting glucagon was correlated with eGFR and UACR (r=–0.112 and r=0.157, respectively; P<0.001), and AUCgla was also correlated with eGFR and UACR (r=–0.267 and r=0.234, respectively; P<0.001). Moreover, 31.7% (n=771) presented with DKD; the prevalence of DKD was 27.3%, 27.6%, 32.5%, and 39.2% in the first (Q1), second (Q2), third (Q3), and fourth quartile (Q4) of fasting glucagon, respectively; and the corresponding prevalence for AUCgla was 25.9%, 22.7%, 33.7%, and 44.4%, respectively. Furthermore, after adjusting for other clinical covariates, the adjusted odds ratios (ORs; 95% confidence intervals) for DKD in Q2, Q3, and Q4 versus Q1 of fasting glucagon were 0.946 (0.697 to 1.284), 1.209 (0.895 to 1.634), and 1.521 (1.129 to 2.049), respectively; the corresponding ORs of AUCgla were 0.825 (0.611 to 1.114), 1.323 (0.989 to 1.769), and 2.066 (1.546 to 2.760), respectively. Additionally, when we restricted our analysis in patients with glycosylated hemoglobin <7.0% (n=471), we found fasting glucagon and AUCgla were still independently associated with DKD.Conclusion: Both increased fasting and postchallenge glucagon levels were independently associated with DKD in T2DM patients.

Hysteretic Behavior of Recycled Aggregate Concrete with Ferronickel Slag-Filled Steel Tubular Columns Subjected to Cyclic Loading

Hua-Yan Chen,Fengxuan Wang,Mianyue Yang,Ai Qi,Guochan Chen,Caisong Luo,Bizhen Wang 한국강구조학회 2023 International Journal of Steel Structures Vol.23 No.1

In order to investigate the hysteretic behavior of recycled aggregate concrete with ferronickel slag-filled steel tubular (RAC-FNSFST) columns, the quasi-static loading was implemented on nine specimens with different replacement ratios (RACs), axial load levels, length–diameter ratios, and diameter–thickness ratios. The hysteretic curves, skeleton curves, deformability, energy dissipation capacity, and stiffness degeneration were studied after loading and failure mechanisms were observed, followed by the construction of FE models for parameter analysis. It is demonstrated that the hysteretic loop curve is full, and the hysteretic performance was not dramatically affected by the replacement ratio of RAC. With axial load level increase, ultimate strength at descending stage degrades quickly, stiffness degeneration accelerates, and hysteretic energy dissipation increases. Stiffness degeneration and hysteresis energy dissipation are enhanced as the length–diameter ratio increases. However, when the diameter–thickness ratio decreases, hysteretic energy dissipation increases, and stiffness degeneration accelerates. In addition, a suitable FE model was established and compared with experimental results. Then a wide range of parameter studies was carried out as a supplement to the experimental study. It is shown that the ultimate strength and ductility of specimens are intimately correlated with the RAC strength, yield strength of steel tube, slenderness ratio, axial load level, and steel ratio.

Fracture behavior of cylindrical sandstone specimens with two pre-existing flaws: experimental investigation and PFC3D simulation

Yan-Hua Huang,Sheng-Qi Yang,Guo-Qing Chen,Bo Meng 한국지질과학협의회 2022 Geosciences Journal Vol.26 No.1

It is important to analyze the rock crack initiation, propagation and coalescence in fractured rock mass. At present, the 3D crack evolution process of pre-flawed rock has not been comprehensively studied during the deformation. In this study, uniaxial compression tests were carried out on cylindrical sandstone specimens with two pre-existing flaws with various ligament inclination angles. The crack evolution process at the front surface of the specimen was analyzed by photographic and AE monitoring. In addition, a 3D discrete element modelling was conducted to explore the spatial crack evolution behaviors during compressive loading. Both the numerical strength and final failure modes of specimens were consistent with those obtained in the laboratory experiment. The numerical simulation results revealed that the internal crack distribution was not always identical along the thickness direction. Two types of crack coalescence were identified. The experimental and numerical results enhance the understanding of 3D crack evolution mechanism of fractured rock under external loading.

Empirical Analysis on the Industrial Productivity in the Electricity·Gas·Water Service Sector

Yan Hua Zhu,Joo Hoon Kang,Sehoon Park 한국산업정보학회 2015 한국산업정보학회논문지 Vol.20 No.4

The early studies indicated that the firm with monopoly power is likely to engage in X-inefficiency such as a managerial slack. The reflection of the X-inefficiency theory has led to the issue that the public sector may be more inefficient than the private sector. In Korea like other many countries the electricity·gas·water service which can be considered as natural monopoly have been provided mostly by the public sector. In order to provide the empirical evidence to the argument that the public sector may be more inefficient than the private sector this paper estimated the four types of Solow residual which is called the total factor productivity in the electricity·gas·water service industry with the associated empirical model and compared its productivity with one in the manufacturing industry. The empirical results do not support the argument that the public sector may be more inefficient or less productive than the private sector.

한국과 중국의 제조산업의 솔로우 잔차항에 관한 비교실증분석

( Yan Hua Zhu ),( Sehoon Park ),( Suk Jung Jeong ) 국제지역학회 2014 국제지역연구 Vol.18 No.1

The Korean economy has accomplished the high rates of economic growth during the last four decades through the export-led growth strategy by the government. It is well recognized that the export in the Korean manufacturing industries has been still leading the Korean economic growth. On the other hand the Chinese economy has also achieved rapidly economic growth since 1990`s. The cyclicality of productivity has been one of the essential issues in economics. Since Solow(1957) developed the dominant approach to the measurement of productivity growth, Solow`s approach, which assumes the perfect competition, the constant returns to scale, and the full use of input factor has been modified particularly in Hall`s(1990) and Basu`s(1996) works. This paper establishes the empirical model based on Hall`s and Basu`s models, estimates 4 types of Solow`s residuals in 8 Korean manufacturing industries and in Chinese economy and analyzes the cyclicality of measured productivity, comparing the productivities in both countries.

Roles of Immunohistochemical Staining in Diagnosing Pulmonary Squamous Cell Carcinoma

Yan, Yue,Zhang, Ya-Xiong,Fang, Wen-Feng,Kang, Shi-Yang,Zhan, Jian-Hua,Chen, Nan,Hong, Shao-Dong,Liang, Wen-Hua,Tang, Yan-Na,He, Da-Cheng,Wu, Xuan,Zhang, Li Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.2

Background: Differentiating morphologic features based on hematoxylin-eosin (HE) staining is the most common method to classify pathological subtypes of non-small-cell lung cancer (NSCLC). However, its accuracy and inter-observer reproducibility in pathological diagnosis of poorly differentiated NSCLC remained to be improved. Materials and Methods: We attempted to explore the role of immunohistochemistry (IHC) staining in diagnosing pulmonary squamous cell carcinoma (SQCC) with poorly differentiated features by HE staining or with elevated serum adenocarcinoma-specific tumor markers (AD-TMs). We also compared the difference of epidermal growth factor receptor (EGFR) mutation rate between patients with confirmed SQCC and those with revised pathological subtype. Logistic regression analyses were used to test the association between different factors and diagnostic accuracy. Results: A total of 132 patients who met the eligible criteria and had adequate specimens for IHC confirmation were included. Pathological revised cases in poor differentiated subgroup, biopsy samples and high-level AD-TMs cases were more than those with high/moderate differentiation, surgical specimens and normal-level AD-TMs. Moreover, biopsy sample was a significant factor decreasing diagnostic accuracy of pathological subtype (OR, 4.037; 95% CI 1.446-11.267, p=0.008). Additionally, EGFR mutation rate was higher in patients with pathological diagnostic changes than those with confirmed SQCC (16.7% vs 4.4%, p=0.157). Conclusions: Diagnosis based on HE staining only might cause pathological misinterpretation in NSCLC patients with poor differentiation or high-level AD-TMs, especially those with biopsy samples. HE staining and IHC should be combined as pathological diagnostic standard. The occurrence of EGFR mutations in pulmonary SQCC might be overestimated.