Analysis of genetic diversity among indigenous landraces from sesame (Sesamum indicum L.) core collection in China as revealed by SRAP and SSR markers

Yan‐xin Zhang,Wei Hua,Lin‐han Wang,Zhuo Che,Xiu‐rong Zhang 한국유전학회 2010 Genes & Genomics Vol.32 No.3

The molecular genetic diversity of 404 indigenous landraces from sesame core collection in China were evaluated by 11SRAP and 3 SSR markers, 175 fragments were generated, of which 126 were polymorphic with an average polymorphism rate of 72%. Jaccard’s genetic similarity coefficients (GS=0.7130), Nei's gene diversity (h=0.2418) and Shannon's Information index (I=0.3847) were calculated, a dendrogram of the 404 landraces was made, landraces from various zones were distributed throughout the dendrogram, accessions from different agro‐ecological zones were indistinguishable by cluster analysis, geographical separation did not generally result in greater genetic distance, a similar pattern was obtained using principal coordinates (PCO) analysis. As to seven agro‐ecological zones, the maximum Nei’s gene diversity (h = 0.2613)and Shannon index (I = 0.3980) values in zone VII indicated that they were genetically more diverse than those in other zones, while the least genetically diverse region was zone III (h = 0.1772, I = 0.2858). Nei's genetic identity and genetic distance among landraces from seven agro‐ecological zones were also analyzed, the genetic relationship of seven zones was inferred using the UPGMA method. This study demonstrated that SRAP and SSR markers were appropriate for evaluation of sesame genetic diversities. There existed extensive genetic diverse among indigenous landraces and the abundance of genetic diversity of landraces in different agro‐ecological zones was various. Understanding of these characteristics of indigenous landraces in China can provide theoretical foundation for further collection, effective protection and reasonable utilization of these sesame landraces in breeding.

Constructing mixed matrix membranes for CO2 separation based on light lanthanide fluoride nanosheets with mesoporous structure

Yanli Zhang,Meixue Zhao,Xu Li,Qingping Xin,Xiaoli Ding,Lizhi Zhao,Hui Ye,Ligang Lin,Hong Li,Yuzhong Zhang 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.125 No.-

The incorporation of porous nano-fillers into mixed matrix membranes (MMMs) has a great impact onCO2 separation. In this study, a series of F-Ln (Ln is the Light Lanthanide, Ln = La, Ce, Pr, Nd) nanosheetswith mesoporous structure was fabricated as a filler in Pebax1657 matrix to improve the performance ofCO2 separation. F-Ln nanosheets are uniformly distributed in the membrane and the mesoporous structurecan construct multiple channels for CO2 fast transport in Pebax/F-Ln MMMs. The pore diameter ofnanosheet is in order: F-La > F-Ce > F-Pr > F-Nd, and the smaller pore diameter of nanosheets is beneficialto improve the CO2 separation performance. In humidified conditions, Pebax/F-Nd-6 MMMs show optimumseparation performance among MMMs, and the CO2 permeability is up to 1265 Barrer and CO2/CH4selectivity is 36.7, which are 2.3 times and 1.9 times higher than pure Pebax and approaches 2019 upperbound. Meanwhile, to demonstrate the potential universal applicability of F-Ln nanosheets in CO2 separation,XLPEO/F-Ce MMMs were fabricated to separate CO2/N2 and the performance exceeds the 2019upper bound. The efficient CO2 separation performance of 2D F-Ln nanosheets with mesoporous structurein membranes reveals the potential application of these nanosheets in industrial CO2 separation.

Efficacy and Safety of Endostar^® Combined with Chemotherapy in Patients with Advanced Soft Tissue Sarcomas

Zhang, Lu-Ping,Liao, Xing-Yun,Xu, Yan-Mei,Yan, Lv-Jun,Yan, Gui-Fang,Wang, Xin-Xin,Duan, Yu-Zhong,Sun, Jian-Guo Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.7

Background: Soft tissue sarcomas (STS) are a heterogeneous group of tumors, and approximately 40-50% of patients with STS develop metastatic disease. The median overall survival of those patients was 12 months and their 5-year survival rate was 8%. Therefore, study on more effective treatment, especially the targeting therapies, is urgently needed. Objective: To evaluate the efficacy and safety of Endostar$^{(R)}$ combined with chemotherapy in patients with advanced STS. Methods: A retrospective case-series study was conducted in Cancer Institute of PLA, Xinqiao Hospital. A total of 71 patients suffering from advanced STS (IIB - IV) were included, of whom 49 cases treated with chemotherapy alone were defined as the control group and the rest 22 cases treated with the traditional chemotherapy combined with Endostar$^{(R)}$ were defined as the test group. The short-term therapeutic effects including objective response rate (ORR), disease control rate (DCR) and safety were evaluated in the two groups. In the follow-up, progression-free survival (PFS) and overall survival (OS) were also observed. Results: In the test and control groups, the ORR was 18.2% and 12.2%, respectively (P=0.767), and the DCR was 86.4% and 61.2%, respectively (P=0.034). The median time to progression in the test and control groups was 120 days and 70 days with significant difference (P = 0.017), while the median overall survival was 452 days and 286 days without significant difference (P=0.503). The one-year survival rate in the test group and control group was 56.2% and 35.4%, respectively, while the two-year survival rate was 30.2% and 26.5%, respectively. No significant difference in the side effects was found between the two groups. Conclusions: Endostar$^{(R)}$ combined with chemotherapy resulted in a higher DCR and longer PFS in the patients with advanced STS, and the toxicity was tolerable.

Protective effects of phillyrin against influenza A virus in vivo

Xin-yan Qu,Qing-jun Li,Hui-min Zhang,Xiao-juan Zhang,Peng-hui Shi,Xiu-juan Zhang,Jing Yang,Zhe Zhou,Sheng-qi Wang 대한약학회 2016 Archives of Pharmacal Research Vol.39 No.7

Influenza A virus infection represents a great threat to public health. However, owing to side effects and the emergence of resistant virus strains, the use of currently available anti-influenza drugs may be limited. In order to identify novel anti-influenza drugs, we investigated the antiviral effects of phillyrin against influenza A virus infection in vivo. The mean survival time, lung index, viral titers, influenza hemagglutinin (HA) protein and serum cytokines levels, and histopathological changes in lung tissue were examined. Administration of phillyrin at a dose of 20 mg/kg/day for 3 days significantly prolonged the mean survival time, reduced the lung index, decreased the virus titers and interleukin-6 levels, reduced the expression of HA, and attenuated lung tissue damage in mice infected with influenza A virus. Taken together, these data showed that phillyrin had potential protective effects against infection caused by influenza A virus.

OsMSR15 encoding a rice C2H2-type zinc finger protein confers enhanced drought tolerance in transgenic Arabidopsis

Xin Zhang,Bin Zhang,Ming Juan Li,Xu Ming Yin,Li Fang Huang,Yan Chun Cui,Man Ling Wang,Xin Jie Xia 한국식물학회 2016 Journal of Plant Biology Vol.59 No.3

Zinc nger proteins (ZFPs) play important roles in plant responses to biotic and abiotic stresses. Through microarray analysis, an Oryza sativa L. multi-stress-responsive gene, OsMSR15, was identied and subsequently cloned from rice Pei’ai 64S (Oryza sativa L.). Expression of OsMSR15 was strongly up-regulated by cold, drought and heat stresses in different tissues at different developmental stages of rice. OsMSR15 contains two C2H2-type zinc nger motifs, a nuclear localization signal (B box), a Leu-rich domain (L-box) and a conserved EAR-motif close to its C-terminus. The OsMSR15-GFP fusion protein was localized to the nucleus. Yeast-one hybrid assay showed that OsMSR15 possesses transcriptional activation ability. Expression of OsMSR15 in Arabidopsis conferred drought tolerance, and transgenic plants showed hypersensitivity to exogenous ABA during the seed germination and post-germination stages. Transgenic plants also showed higher levels of free proline, less electrolyte leakage and increased expressions of a number of stress-responsive genes, including LEA3, RD29A, DREB1A and P5CS1 under drought stress. The obtained results indicate that OsMSR15 is an important regulator involved in plant response to drought stress.

중국전통가구와 현대 중식 가구에 대한 비교분석 및 디자인방향에 대한 연구

장신안(Zhang, Xin Yan),곽대영(Kwak, Dae Young) 한국디자인문화학회 2017 한국디자인문화학회지 Vol.23 No.2

현대 중식 가구는 전통 가구를 기반으로 중국의 전통과 현대의 문화적 요소를 융합하여 디자인한 신세대 공예품으로써 사람들의 환영을 받고 있다. 그러나 현대 중식 가구는 아직 안정된 체계를 형성하지 못하여 이에 관한 이론 연구가 많지 않다. 본 논문에서는 문헌 조사와 사례 분석의 연구 방법을 통하여 현대 중식 가구에 관한 연구를 진행하였다. 먼저 현대 중식 가구는 중국의 고전적 분위기가 있으면서 명확한 현대적 특징을 지니고 있는 가구라고 정의를 내린다. 고전적 분위기의 의미는 동전(铜钱)과 같은 중국의 전통문화 요소를 가지고 있다는 뜻이다. 현대 중식 가구의 문제점 중에 하나는 바로 적당한 문화 요소를 계승하지 못한다는 것이다. 현대 중국인들은 전통식 심미적 취미를 가지면서 현대식 가구의 편리함과 같은 장점을 선호한다. 이러한 소비자의 요구를 고려하여, 한스 베그너, 주소걸(朱小杰), 반목가구(半木家具) 등 대표적인 디자인 사례를 조사 분석하고, 현대 중식 가구의 조형, 기능, 재료, 구조의 특징을 밝혔다. 이어서 현대 중식 가구의 디자인 방향을 제시하였다. 결론적으로 전통 중식 가구를 바탕으로 한 단순화, 기능성과 실용성의 강조화, 실용적 재료의 일반화와 전통 구조의 계승 및 보존화 등 현대 중식 가구가 전통 중식 가구의 장점을 계승하면서 현대적 요소를 선택하고 처리 하는 방법을 제시하였다. Modern Chinese style furniture is a new type of art that mixes traditional and modern elements together in order to recreate on the basis of traditional furniture. It is popular among the public. However, the modern Chinese furniture has not yet become a stable and mature system, and there are few theoretical researches about it. This paper focuses on modern Chinese furniture with the method of document survey and project analysis. First of all, this paper defines the concept of modern Chinese furniture. Namely, Chinese style furniture has the characteristics of Chinese traditional culture trait and contemporary soceity at the same time. Traditional Chinese culture traint refers to the usages of imagery in traditional Chinese are applied to modern Chinese furniture. However, the reasonable inheritance of traditional culture is one of the drawbacks in modern Chinese furniture design. Modern Chinese people are fond of the features of traditional furniture as well as the convenience of modern furniture. According to some famous home design examples such as Half Wood, Hans and Zhu Xiao jie, I would like to analyse shapes, materials, functions and structures of modern Chinese style furniture after understanding the demand of consumers. Furthermore, I incline to introduce the prospects of modern Chinese style furniture development in the future. Moreover, this paper presents the point that modern Chinese style furniture design should be simplified on the foundation of traditional ones. In addition, I also make some suggestions about how to strenthen its functional, practical and behavior induced characteristics and complement each other in the conclusion.

변두리화된 밑바닥 인물 서사 <먼지로 들어가다(隱入塵煙)>의 공간 서사 해독

장옌신 ( Zhang Yan Xin ) 한국영화교육학회 2023 영화교육연구 Vol.17 No.2

영화 < 먼지로 들어가다(隱入塵煙, Return to Dust) >는 72번째 베를린 국제 영화제에서 메인 경쟁 부서의 후보자로 선정되었다. 전문 행사로 인정되었을 뿐만 아니라 관객의 명성에서도 높은 평가를 받았다. 가이치(蓋琪)는 『현대 영화』에서 저널 표현을 출판하여 문화적 주제의 실제 수준에서 외부적으로 간주 될 수 있다고 생각하였다. 영화는 전체 서사에서 중국 사회 변혁시대에 생활하는 일반 농민들이 직면한 현실적 곤경, 농업 문명에 대한 그리움과 애도를 보여주는 동시에, 그리고 공업 문명과 상업 문명에 대한 무시와 소외를 보여주고 있다. 이제 또 다른 새로운 시골 문명을 창조하려고 하였다. The movie Return to Dust was selected as a candidate for the main competition department at the 72nd Berlin International Film Festival. It was not only recognized as a professional event, but also highly praised by the audience's reputation. Gaiqi thought that it could be considered externally at the actual level of cultural topics by publishing journal expressions in Modern Film. In the entire narrative, the film shows the real plight faced by ordinary farmers living in the era of social transformation in China, longing and mourning for agricultural civilization, and neglect and alienation of industrial and commercial civilizations. Now trying to create another new rural civilization.

Disruption of endothelial barrier function is linked with hyposecretion and lymphocytic infiltration in salivary glands of Sjögren's syndrome

Cong, Xin,Zhang, Xue-Ming,Zhang, Yan,Wei, Tai,He, Qi-Hua,Zhang, Li-Wei,Hua, Hong,Lee, Sang-Woo,Park, Kyungpyo,Yu, Guang-Yan,Wu, Li-Ling Elsevier 2018 Biochimica et biophysica acta. Molecular basis of Vol.1864 No.10

<P><B>Abstract</B></P> <P>Sjögren's syndrome (SS) is an inflammatory autoimmune disease that causes hyposecretion in salivary glands. Endothelial tight junctions (TJs) play crucial roles in salivation and barrier function of blood vessels. However, whether the alteration of endothelial TJs were involved in pathogenesis of SS was still unknown. Here, the ultrastructure and function of endothelial TJs in submandibular glands (SMGs) were detected by transmission electron microscopy and in vivo paracellular permeability assay in different aged NOD mouse model for SS. CFSE-labeled lymphocytes were injected into tail vein to trace the infiltration, while claudin-5 expression and distribution were detected by immunofluorescence, qRT-PCR, and western blot. Results showed that the stimulated salivary flow rate was gradually decreased and lymphocytic infiltration was found as age increased in 12- and 21-week-old NOD mice, but not 7-week-old NOD mice. Blood vessels were dilated, while endothelial TJ width and paracellular tracer transport were increased in 12-week-old NOD mice. Moreover, the injected CFSE-labeled lymphocytes were observed in SMGs of 12-week-old NOD mice. Claudin-5 level was increased and relocalized from the apical portion of neighboring endothelial cells to lateral membranes and cytoplasm in 12-week-old NOD mice. Additionally, the alteration of claudin-5 expression and distribution was further confirmed in labial salivary glands and bilateral parotid glands from SS patients. In cultured human microvessel endothelial cell line (HMEC-1), IFN-γ stimulation significantly increased claudin-5 expression. Taken together, we identified that the endothelial TJ barrier was disrupted and contributed to the development of salivary hyposecretion and lymphocytic infiltration in SS.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Endothelial tight junction barrier is disrupted in hyposecretory submandibular glands from Sjögren's syndrome mouse model </LI> <LI> The disrupted salivary endothelial barrier is linked with lymphocytic infiltration in Sjögren's syndrome mouse model </LI> <LI> The redistribution of claudin-5 is responsible for disrupted endothelial barrier in salivary glands from Sjögren's syndrome </LI> </UL> </P>

Tunicamycin enhances TRAIL-induced apoptosis by inhibition of cyclin D1 and the subsequent downregulation of survivin

Hai-Yan Zhang,Zhen-Xian Du,Bao-Qin Liu,Yan-Yan Gao,Xin Meng,Yifu Guan,Wei-Wei Deng,Hua-Qin Wang 생화학분자생물학회 2009 Experimental and molecular medicine Vol.41 No.5

TNF-related apoptosis-inducing ligand (TRAIL) has been proposed as a promising cancer therapy that preferentially induces apoptosis in cancer cells, but not most normal tissues. However, many cancers are resistant to TRAIL by mechanisms that are poorly understood. In this study, we showed that tunicamycin, a naturally occurring antibiotic, was a potent enhancer of TRAIL-induced apoptosis through downregulation of survivin. The tunicamycin-mediated sensitization to TRAIL was efficiently reduced by forced expression of survivin, suggesting that the sensitization was mediated at least in part through inhibition of survivin expression. Tunicamycin also repressed expression of cyclin D1, a cell cycle regulator commonly overexpressed in thyroid carcinoma. Furthermore, silencing cyclin D1 by RNA interference reduced survivin expression and sensitized thyroid cancer cells to TRAIL; in contrast, forced expression of cyclin D1 attenuated tunicamycin-potentiated TRAIL-induced apoptosis via over-riding downregulation of survivin. Collectively, our results demonstrated that tunicamycin promoted TRAIL-induced apoptosis, at least in part, by inhibiting the expression of cyclin D1 and subsequent survivin. Of note, tunicamycin did not sensitize the differentiated thyroid epithelial cells to TRAIL-induced apoptosis. Thus, combined treatment with tunicamycin and TRAIL may offer an attractive strategy for safely treating resistant thyroid cancers. TNF-related apoptosis-inducing ligand (TRAIL) has been proposed as a promising cancer therapy that preferentially induces apoptosis in cancer cells, but not most normal tissues. However, many cancers are resistant to TRAIL by mechanisms that are poorly understood. In this study, we showed that tunicamycin, a naturally occurring antibiotic, was a potent enhancer of TRAIL-induced apoptosis through downregulation of survivin. The tunicamycin-mediated sensitization to TRAIL was efficiently reduced by forced expression of survivin, suggesting that the sensitization was mediated at least in part through inhibition of survivin expression. Tunicamycin also repressed expression of cyclin D1, a cell cycle regulator commonly overexpressed in thyroid carcinoma. Furthermore, silencing cyclin D1 by RNA interference reduced survivin expression and sensitized thyroid cancer cells to TRAIL; in contrast, forced expression of cyclin D1 attenuated tunicamycin-potentiated TRAIL-induced apoptosis via over-riding downregulation of survivin. Collectively, our results demonstrated that tunicamycin promoted TRAIL-induced apoptosis, at least in part, by inhibiting the expression of cyclin D1 and subsequent survivin. Of note, tunicamycin did not sensitize the differentiated thyroid epithelial cells to TRAIL-induced apoptosis. Thus, combined treatment with tunicamycin and TRAIL may offer an attractive strategy for safely treating resistant thyroid cancers.

Comparison of Liver Transplantation and Liver Resection for Hepatocellular Carcinoma Patients with Portal Vein Tumor Thrombus Type I and Type II

Jia-Yu Lv,Ning-Ning Zhang,Ya-Wei Du,Ying Wu,Tian-Qiang Song,Ya-Min Zhang,Yan Qu,Yu-Xin Liu,Jie Gu,Ze-Yu Wang,Yi-Bo Qiu,Bing Yang,Da-Zhi Tian,Qing-Jun Guo,Li Zhang,Ji-San Sun,Yan Xie,Zheng-Lu Wang,Xin 연세대학교의과대학 2021 Yonsei medical journal Vol.62 No.1

Purpose: The aim of this study was to compare the efficacy of liver transplantation (LT) and liver resection (LR) for hepatocellularcarcinoma (HCC) patients with portal vein tumor thrombus (PVTT) and to investigate risk factors affecting prognosis. Materials and Methods: A total of 94 HCC patients with PVTT type I (segmental PVTT) and PVTT type II (lobar PVTT) were involvedand divided into LR (n=47) and LT groups (n=47). Recurrence-free survival (RFS) and overall survival (OS) were comparedbefore and after inverse probability of treatment weighting (IPTW). Prognostic factors for RFS and OS were explored. Results: Two treatment groups were well-balanced using IPTW. In the entire cohort, LT provided a better prognosis than LR. Among patients with PVTT type I, RFS was better with LT (p=0.039); OS was not different significantly between LT and LR(p=0.093). In subgroup analysis of PVTT type I patients with α-fetoprotein (AFP) levels >200 ng/mL, LT elicited significantly longermedian RFS (18.0 months vs. 2.1 months, p=0.022) and relatively longer median OS time (23.6 months vs. 9.8 months, p=0.065). Among patients with PVTT type II, no significant differences in RFS and OS were found between LT and LR (p=0.115 and 0.335,respectively). Multivariate analyses showed treatment allocation (LR), tumor size (>5 cm), AFP and aspartate aminotransferase(AST) levels to be risk factors of RFS and treatment allocation (LR), AFP and AST as risk factors for OS. Conclusion: LT appeared to afford a better prognosis for HCC with PVTT type I than LR, especially in patients with AFP levels>200 ng/mL.