Central-Located Massive Tumor With Inflow And Outflow Reconstruction For Massive Hepatectomy - A Case Report

( Xue-yin Shen ),( Xu-guang Hu ),( Sung-yeon Hong ),( Bong-wan Kim ),( Hee-jung Wang ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: Right trisectionectomy was the most effective curative treatment with massive tumor located in the right lobe especially central location of liver. Due to the compressing or even invasion from huge tumor to main branches of portal vein, hepatic vein and bile duct, adequate consideration of inflow and outflow reconstruction during surgery was needed and vessels acquired from cadaveric donor or artificial should be prepared preoperatively. Methods: We present the case of a 59-year-old man who found a 10 cm malignant mass in the right lobe with the invasion of right anterior portal vein, right hepatic vein and middle hepatic vein. By performing right portal vein embolization, left lateral section volume was increased from 26.5% to 37.3%, satisfy the condition of right trisectionectomy. Outflow reconstruction was performed using pericardium, which was acquired from previous cadaveric donor. Results: The recovery process was smooth, patient was discharged in POD 58 without any complication, Conclusions: Preoperatively, sufficient consideration of using allograft to perform inflow or outflow reconstruction is needed in patient with vascular invasion. Postoperatively, regular hepatic mesenteric doppler F/U is needed. Usage of allograft is limited in the hospital where cadaveric donor liver transplantation is feasible. Thus, artificial graft should be prepared in the vascular invasion cases.

Can we delineate preoperatively the right and ventral margins of caudate lobe of the liver?

Xue-Yin Shen,Hee-Jung Wang,Bong-Wan Kim,Sung-Yeon Hong,Mi-Na Kim,Xu-Guang Hu 대한외과학회 2019 Annals of Surgical Treatment and Research(ASRT) Vol.97 No.3

Purpose: Complete removal of the caudate lobe, which is sometimes necessary, is accomplished via isolated caudate lobectomy or hepatectomy that includes the caudate lobe. It is impossible, however, to confirm the right and ventral margins of the caudate lobe by preoperative imaging. This study was undertaken to determine whether we could identify the right and ventral margins of the caudate lobe preoperatively using Synapse 3D visualization software. Methods: Ninety-four preoperative 3-dimensional (3D) computed tomographic images (1-mm slices) of the liver from candidate donors were examined. The images of the caudate lobe were subjected to a counter-staining method according to Synapse 3D to delineate their dimensions. We first examined whether the right margin of the caudate lobe exceeded the plane formed by the root of the right hepatic vein (RHV) and the right side of the inferior vena cava (IVC). Second, we determined whether the ventral margin of the caudate lobe exceeded the plane formed by the root of the middle hepatic vein (MHV) and the root of the RHV. Results: For the right margin, 17 cases (18%) exceeded the RHV-IVC plane by a mean of 10.2 mm (range, 2.4–27.2 mm). For the ventral margin, 28 cases (30%) exceeded the MHV-RHV plane by a mean of 17.4 mm (range, 1.2–49.1 mm). Conclusion: Evaluating the anatomy of caudate lobe using Synapse 3D preoperatively could be helpful for more precise anatomical resection of the caudate lobe.

Molecular classification of hepatocellular adenoma: A single-center experience

Xue-Yin Shen,Xu-Guang Hu,Young-Bae Kim,Mi-Na Kim,Sung-Yeon Hong,Bong-Wan Kim,Hee-Jung Wang 한국간담췌외과학회 2019 Annals of hepato-biliary-pancreatic surgery Vol.23 No.2

Backgrounds/Aims: Hepatocellular adenoma (HCA) is a rare benign tumor that has a risk of malignant transformation into hepatocellular carcinoma (HCC) and bleeding. The aim of this study was to analyze the characteristics of HCA by performing molecular classification. Methods: We retrospectively collected data from nine patients who were diagnosed with HCA from 1995 to 2016. The patients underwent liver surgery due to the existence of clinical symptoms. Immunohistochemical (IHC) staining was performed to classify the subgroups of HCA. Results: Four patients with both β-catenin and inflammation were classified as β-IHCA. Two patients were defined as β-HCA. Two patients were classified as HHCA. Only one patient was defined as IHCA. None of the patients had unclassified HCA. Seven of nine patients had a malignant transformation. By comparing the characteristics of HCA between two groups, we found the mean tumor size in the malignant transformation group was greater than the non-malignant transformation group. Conclusions: Taken together, the mean tumor size and activation of catenin β1 mutation status might be the risk factors for the malignant transformation of HCA into HCC. Moreover, IHCA without the catenin β1 mutation could also have a possibility of malignant transformation into HCC.

Identification of genes involved in inbreeding depression of reproduction in Langshan chickens

Xue Qian,Li Guohui,Cao Yuxia,Yin Jianmei,Zhu Yunfen,Zhang Huiyong,Zhou Chenghao,Shen Haiyu,Dou Xinhong,Su Yijun,Wang Kehua,Zou Jianmin,Han Wei 아세아·태평양축산학회 2021 Animal Bioscience Vol.34 No.6

Objective: Inbreeding depression of reproduction is a major concern in the conservation of native chicken genetic resources. Here, based on the successful development of strongly inbred (Sinb) and weakly inbred (Winb) Langshan chickens, we aimed to evaluate inbreeding effects on reproductive traits and identify candidate genes involved in inbreeding depression of reproduction in Langshan chickens. Methods: A two-sample t-test was performed to estimate the differences in phenotypic values of reproductive traits between Sinb and Winb chicken groups. Three healthy chickens with reproductive trait values around the group mean values were selected from each of the groups. Differences in ovarian and hypothalamus transcriptomes between the two groups of chickens were analyzed by RNA sequencing (RNA-Seq). Results: The Sinb chicken group showed an obvious inbreeding depression in reproduction, especially for traits of age at the first egg and egg number at 300 days (p<0.01). Furthermore, 68 and 618 differentially expressed genes (DEGs) were obtained in the hypothalamus and ovary between the two chicken groups, respectively. In the hypothalamus, DEGs were mainly enriched in the pathways related to vitamin metabolism, signal transduction and development of the reproductive system, such as the riboflavin metabolism, Wnt signaling pathway, extracellular matrix-receptor interaction and focal adhesion pathways, including stimulated by retinoic acid 6, serpin family F member 1, secreted frizzled related protein 2, Wnt family member 6, and frizzled class receptor 4 genes. In the ovary, DEGs were significantly enriched in pathways associated with basic metabolism, including amino acid metabolism, oxidative phosphorylation, and glycosaminoglycan degradation. A series of key DEGs involved in folate biosynthesis (gamma-glutamyl hydrolase, guanosine triphosphate cyclohydrolase 1), oocyte meiosis and ovarian function (cytoplasmic polyadenylation element binding protein 1, structural maintenance of chromosomes 1B, and speedy/RINGO cell cycle regulator family member A), spermatogenesis and male fertility (prostaglandin D2 synthase 21 kDa), Mov10 RISC complex RNA helicase like 1, and deuterosome assembly protein 1) were identified, and these may play important roles in inbreeding depression in reproduction. Conclusion: The results improve our understanding of the regulatory mechanisms underlying inbreeding depression in chicken reproduction and provide a theoretical basis for the conservation of species resources. Objective: Inbreeding depression of reproduction is a major concern in the conservation of native chicken genetic resources. Here, based on the successful development of strongly inbred (Sinb) and weakly inbred (Winb) Langshan chickens, we aimed to evaluate inbreeding effects on reproductive traits and identify candidate genes involved in inbreeding depression of reproduction in Langshan chickens.Methods: A two-sample t-test was performed to estimate the differences in phenotypic values of reproductive traits between Sinb and Winb chicken groups. Three healthy chickens with reproductive trait values around the group mean values were selected from each of the groups. Differences in ovarian and hypothalamus transcriptomes between the two groups of chickens were analyzed by RNA sequencing (RNA-Seq).Results: The Sinb chicken group showed an obvious inbreeding depression in reproduction, especially for traits of age at the first egg and egg number at 300 days (p<0.01). Furthermore, 68 and 618 differentially expressed genes (DEGs) were obtained in the hypothalamus and ovary between the two chicken groups, respectively. In the hypothalamus, DEGs were mainly enriched in the pathways related to vitamin metabolism, signal transduction and development of the reproductive system, such as the riboflavin metabolism, Wnt signaling pathway, extracellular matrix-receptor interaction and focal adhesion pathways, including stimulated by retinoic acid 6, serpin family F member 1, secreted frizzled related protein 2, Wnt family member 6, and frizzled class receptor 4 genes. In the ovary, DEGs were significantly enriched in pathways associated with basic metabolism, including amino acid metabolism, oxidative phosphorylation, and glycosaminoglycan degradation. A series of key DEGs involved in folate biosynthesis (gamma-glutamyl hydrolase, guanosine triphosphate cyclohydrolase 1), oocyte meiosis and ovarian function (cytoplasmic polyadenylation element binding protein 1, structural maintenance of chromosomes 1B, and speedy/RINGO cell cycle regulator family member A), spermatogenesis and male fertility (prostaglandin D2 synthase 21 kDa), Mov10 RISC complex RNA helicase like 1, and deuterosome assembly protein 1) were identified, and these may play important roles in inbreeding depression in reproduction.Conclusion: The results improve our understanding of the regulatory mechanisms underlying inbreeding depression in chicken reproduction and provide a theoretical basis for the conservation of species resources.

Oxidation of cyclopentene catalyzed by tungsten-substituted molybdophosphoric acids

Jinjuan Xue,Haixia Li,Dongzhi Zhang,Tingshun Jiang,Longbao Yu,Yutang Shen,Hengbo Yin 한국화학공학회 2009 Korean Journal of Chemical Engineering Vol.26 No.3

A series of Keggin type tungsten-substituted molybdophosphoric acids (H3PMo12−nWnO40·XH2O) were synthesized and characterized by ICP-AES, FT-IR, TG-DSC, and XRD. The tungsten substitution extent significantly affected their catalytic activity in the oxidation of cyclopentene and the selectivity of the resultant products. The tungsten-substituted molybdophosphoric acids with tungsten substitution numbers in a range of 3-6.8 exhibited high catalytic activity in the oxidation of cyclopentene. After reaction for 8 h, the conversion of cyclopentene was up to 97%; the oxidation products mainly consisted of glutaraldehyde, cis-1,2-cyclopentanediol and trans-1,2-cyclopentanediol with the yields of ca. 23%, 27%, and 45%, respectively.

Nonionic Amphiphilic Surfactant Conjuncted Polyethyleneimine as a New and Highly Efficient Non-viral Gene Carrier

Dong Feng Yin,Cang Chu,Xue Ying Ding,Jing Gao,Hao Zou,Shen Gao 한국고분자학회 2009 Macromolecular Research Vol.17 No.1

18F-fluorodeoxyglucose PET/CT as an independent predictor for patients with hepatocellular carcinoma combined with major portal vein tumor thrombus

Xu-Guang Hu,Xue-Yin Shen,Jin-Niang Nan,In-Gyu Kim,Joon-Kee Yoon,Sung-Yeon Hong,Mi-Na Kim,Bong-Wan Kim,Hee-Jung Wang 대한외과학회 2020 Annals of Surgical Treatment and Research(ASRT) Vol.99 No.1

Purpose: Hepatocellular carcinoma (HCC) patients with major portal vein tumor thrombosis (mPVTT) complications were generally characterized by extremely poor prognoses. The aim of this study was to explore the role of 18F-fluorodeoxyglucose (18F-FDG) PET/CT imaging in predicting HCC complicated by mPVTT. Methods: Five hundred one HCC patients received surgery in our hospital during November 2008 to December 2014, among which 32 patients (6.4%) were diagnosed as HCC complicated by mPVTT. Six cases were excluded for reasons of complex medical conditions, including 2 cases of salvage liver transplantation, 2 cases of re-resection, 1 case of mPVTT combined with inferior vina cava tumor thrombosis, and 1 case of residual portal vein tumor thrombosis. Ultimately, 26 cases were enrolled in this study. The maximal tumor standardized uptake value (SUVmax) was identified as a predictive factor and detected. The univariate and multivariate regression analyses were performed to identify the prognostic factors for recurrence-free survival (RFS) and overall survival (OS) of HCC patients complicated by mPVTT. Results: Our results showed that the median OS was 16 months. The 1-, 3-, and 5-year cumulative OS was 55.6%, 31.7%, and 31.7%, respectively. The multivariate regression analysis revealed that SUVmax ≥ 4.65 was the only independent risk factor for RFS and OS. Conclusion: SUVmax was an independent predictor for RFS and OS of patients suffering from both HCC and mPVTT. L ow SUVmax could serve as an effective factor for selecting candidates with low recurrence risks and for helping with improving patient survival after surgical resection.

Polymorphisms of TERT and CLPTM1L and the Risk of Hepatocellular Carcinoma in Chinese Males

Su, Ling-Yan,Li, Xue-Lian,Shen, Li,Zhang, Yue,Zhao, Meng-Meng,Yin, Zhi-Hua,Su, Hong-Ying,Zhou, Bao-Sen Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19

Background: Telomerase reverse transcriptase (TERT) and cleft lip and palate trans-membrane 1 like (CLPTM1L) genes located on chromosome 5p15.33 are known to influence the susceptibility to various cancers. Here, we examined the association of TERT and CLPTM1L single nucleotide polymorphisms (SNPs) with hepatocellular carcinoma (HCC). Materials and Methods: Genotyping of TERT SNP rs2736098 and CLPTM1L SNP rs401681 was performed using TaqMan allelic discrimination assays in a case-control study of 201 HCC cases and 210 controls in a Chinese male population. Odds ratios (ORs) and 95% confidence intervals (CIs) were estimated using logistic regression analyses. Results: Both the rs2736098 T allele of TERT and the rs401681 T allele of CLPTM1L were associated with a significantly increased risk of HCC (adjusted odds ratio [OR]=1.605, 95% confidence interval [CI]=1.164-2.213; adjusted OR=1.399, 95%CI=1.002-1.955, respectively). Individuals carrying both TERT and CLPTM1L risk genotypes had an even higher risk of HCC (adjusted OR=4.420, 95%CI= 2.319-8.425). The TERT rs2736098 T allele was also significantly associated with the level of the HCC clinical indicator alpha-fetoprotein (P=0.026). Conclusions: Our results show that genetic variants of TERT and CLPTM1L may contribute to HCC susceptibility in Chinese males.

The 7th/8th American Joint Committee on Cancer and the Modified Union for International Cancer Control Staging System for Hepatocellular Carcinoma

김인규,Xu-Guang Hu,왕희정,김봉완,홍성연,Xue-Yin Shen 연세대학교의과대학 2019 Yonsei medical journal Vol.60 No.2

Purpose: Although many staging systems have been proposed for hepatocellular carcinoma (HCC), there is no globally acceptedsystem due to the extreme heterogeneity of the disease. We aimed to compare the results of the 7th/8th American Joint Committeeon Cancer (AJCC) and the modified Union for International Cancer Control (mUICC) staging systems in patients with HCC. Materials and Methods: We collected data from 792 patients who underwent hepatic resection at our center. The Kaplan-Meiermethod was used to determine disease-free survival and overall survival. To evaluate homogeneity, ‘-2 log likelihood’ was calculatedusing Cox proportional hazards regression. To measure discriminatory ability, the linear trend chi method and the Cochran-Armitage test for trend were used. The ability to accurately predict survival was verified by cross-validation analysis. Results: Kaplan-Meier curves for disease-free survival and overall survival showed mUICC to be superior to the 7th/8th AJCC. The homogeneity test indicated that mUICC was the best for both disease-free survival and overall survival. In the discriminatoryability test, the chi-square value of mUICC was the best for disease-free survival, while the 7th AJCC had the best value for overallsurvival. In the cross-validation analysis, all three staging systems had significant predictive power. Conclusion: mUICC seemed to be superior to the 7th/8th AJCC after analyzing the data of our surgical patients, although thegeographic heterogeneity of HCC might result in differences between the staging systems. We believe that, while the three stagingsystems allow for the clear stratification of patients into prognostic groups, mUICC may be more appropriate in HCC.

Aldol condensation of acetic acid with formaldehyde to acrylic acid over SiO2-, SBA-15-, and HZSM-5-supported V-P-O catalysts

Jing Hua,Zhipeng Lu,Hengbo Yin,Wuping Xue,Aili Wang,Lingqin Shen,Shuxin Liu 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.40 No.-

Aldol condensation of acetic acid with formaldehyde to acrylic acid was catalyzed by the SiO2-, SBA-15-,and HZSM-5-supported vanadium phosphorous oxide (V-P-O) catalysts prepared by the incipientwetness impregnation. The supports obviously increased the surface areas and changed the acid–baseproperties of the as-prepared catalysts. V-P-O/SBA-15 catalysts with high acid and alkali quantitiesexhibited high catalytic activities for the aldol condensation of acetic acid with formaldehyde to acrylicacid. When the V-P-O/SBA-15 catalyst with the P/V mole ratio of 2.0:1 catalyzed the reaction at 330–370 8C, the acrylic acid selectivities were 90.8–70.2% at the formaldehyde conversions of 14.3–68.7%.