Isolation and Molecular Characterization of a New CRT Binding Factor Gene from Capsella bursa-pastoris

Wang, Xinglong,Liu, Li,Liu, Sixiu,Sun, Xiaoqing,Deng, Zhongxiang,Pi, Yan,Sun, Xiaofen,Tang, Kexuan Korean Society for Biochemistry and Molecular Biol 2004 Journal of biochemistry and molecular biology Vol.37 No.5

A new CRT binding factor (CBF) gene designated Cbcbf25 was cloned from Capsella bursa-pastoris, a wild grass, by the rapid amplification of cDNA ends (RACE). The full-length cDNA of Cbcbf25 was 898 bp with a 669 bp open reading frame (ORF) encoding a putative DRE/CRT (LTRE)-binding protein of 223 amino acids. The predicted CbCBF25 protein contained a potential nuclear localization signal (NLS) in its N-terminal region followed by an AP2 DNA-binding motif and a possible acidic activation domain in the C-terminal region. Bioinformatic analysis revealed that Cbcbf25 has a high level of similarity with other CBF genes like cbf1, cbf2, and cbf3 from Arabidopsis thaliana, and Bncbf5, Bncbf7, Bncbf16, and Bncbf17 from Brassica napus. A cold acclimation assay showed that Cbcbf25 was expressed immediately after cold triggering, but this expression was transient, suggesting that it concerns cold acclimation. Our study implies that Cbcbf25 is an analogue of other CBF genes and may participate in cold-response, by for example, controlling the expression of cold-regulated genes or increasing the freezing tolerance of plants.

Design of Active Disturbance Rejection Control for Inductive Power Transfer Systems

Wang, Yanan,Dong, Lei,Liao, Xiaozhong,Ju, Xinglong,Xiao, Furong The Korean Institute of Power Electronics 2018 JOURNAL OF POWER ELECTRONICS Vol.18 No.5

The control design of inductive power transfer (IPT) systems has attracted a lot of attention in the field of wireless power transmission. Due to the high-order resonant networks and multiple loads in IPT systems, a simplified model of an IPT system is preferred for analysis and control design, and a controller with strong robustness is required. Hence, an active disturbance rejection control (ADRC) for IPT systems is proposed in this paper. To realize the employment of ADRC, firstly a small-signal model of an LC series-compensative IPT system is derived based on generalized state-space averaging (GSSA), then the ADRC is implemented in the designed IPT system. The ADRC not only provides superior robustness to unknown internal and external disturbances, but also requires few knowledge of the IPT system. Due to the convenient realization of ADRC, the designed IPT system retains its simple structure without any additional circuits. Finally, a frequency domain analysis and experimental results have validated the effectiveness of the employed ADRC, especially its robustness in the presence of frequency drifts and other common disturbances.

Design of Active Disturbance Rejection Control for Inductive Power Transfer Systems

Yanan Wang,Lei Dong,Xiaozhong Liao,Xinglong Ju,Furong Xiao 전력전자학회 2018 JOURNAL OF POWER ELECTRONICS Vol.18 No.5

The control design of inductive power transfer (IPT) systems has attracted a lot of attention in the field of wireless power transmission. Due to the high-order resonant networks and multiple loads in IPT systems, a simplified model of an IPT system is preferred for analysis and control design, and a controller with strong robustness is required. Hence, an active disturbance rejection control (ADRC) for IPT systems is proposed in this paper. To realize the employment of ADRC, firstly a small-signal model of an LC series-compensative IPT system is derived based on generalized state-space averaging (GSSA), then the ADRC is implemented in the designed IPT system. The ADRC not only provides superior robustness to unknown internal and external disturbances, but also requires few knowledge of the IPT system. Due to the convenient realization of ADRC, the designed IPT system retains its simple structure without any additional circuits. Finally, a frequency domain analysis and experimental results have validated the effectiveness of the employed ADRC, especially its robustness in the presence of frequency drifts and other common disturbances.

The effects of light colour on female rabbit reproductive performance and the expression of key genes in follicular development

Xiaoqing Pan,Xinglong Wang,Le Shao,Jie Yang,Feng Qin,Jian Li,Xia Zhang,Pin Zhai 한국축산학회 2022 한국축산학회지 Vol.64 No.3

The purpose of this study was to analyse the effects of light colour on rabbit reproductive performance and the expression of key follicular development genes. Rabbits (n = 1,068, 5 months old, 3.6–4.4 kg live body weight) were divided randomly into four groups, housed individually in wire mesh cages and exposed to red, green, blue, and white light-emitting diode (LED) light (control). The lighting schedule was 16 L : 8 D-15 d / 150 lx / 6:00 am–22:00 pm (3 d preartificial insemination to 12 d postartificial insemination). Red light and white light affected the conception rate and kindling rate and increased the total litter size at birth (p < 0.05). The effects of red light on litter size at weaning, litter weight at weaning, and individual weight at weaning increased compared with the green and blue groups. The effects of red light on live litter size at birth were increased compared with those in the blue group (p < 0.05). Compared to white light, green and blue light reduced the number of secondary follicles (p < 0.05). Compared to red light, green and blue light reduced the number of tertiary follicles (p < 0.05). Compared with white light, red LED light resulted in greater ovarian follicle stimulating hormone receptor and luteinizing hormone receptor mRNA expression (p < 0.05). Compared with green and blue LED light, red LED light resulted in greater B-cell lymphom-2 mRNA expression (p < 0.05). Compared with green LED light, red LED light inhibited FOXO1 mRNA expression in rabbit ovaries (p < 0.05). Red light can affect the reproductive performance of female rabbits and the expression of key genes for follicular development.

Effect of MXene nanosheets attached to carbonyl iron microspheres on the performance and stability of magnetorheological fluid

Yuxi Sun,Yu Wang,Huaxia Deng,Min Sang,XingLong Gong 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.114 No.-

To improve the sedimentation stability of carbonyl iron (CI)-based magnetorheological fluid (MRF), anovel MRF with CI/MXene particles as dispersed phase was prepared. CI/MXene particles were preparedby attaching the lightweight MXene nanosheets onto the surface of CI particles (CIPs) as an additive. Themicro-morphology, crystal structure and element composition of CI/MXene particles were analyzed bySEM, XRD and XPS characterization respectively, which confirmed the successful synthesis of CI/MXene particles. CI/MXene-based MRF possessed considerable high magnetorheological (MR) effect,and the magneto-induced shear stress was up to 49 kPa. More importantly, the sedimentation ratio ofCI/MXene-based MRF was further characterized to evaluate its stability. Experimental results showedthat the sedimentation ratio of MRF increased from 75.7% to 98.0% after mixing CIPs with 3 wt%MXene nanosheets. The sedimentation ratio of CI/MXene-based MRF increased by 29.5% compared toCI-based MRF. As a result, the addition of MXene nanosheets significantly improved the sedimentationstability of MRF while maintained high MR effect.

Effect of 3C-SiC Intermediate Layer in GaN-Based Light Emitting Diodes Grown on Si(111) Substrate

Youhua Zhu,Meiyu Wang,Yi Li,Shuxin Tan,Honghai Deng,Xinglong Guo,Haihong Yin,Takashi Egawa 대한금속·재료학회 2017 ELECTRONIC MATERIALS LETTERS Vol.13 No.2

GaN-based light emitting diodes (LEDs) have been grown by metalorganicchemical vapor deposition on Si(111) substrate with and without3C-SiC intermediate layer (IL). Structural property has been characterizedby means of atomic force microscope, X-ray diffraction, andtransmission electron microscope measurements. It has been revealedthat a significant improvement in crystalline quality of GaN and superlatticeepitaxial layers can be achieved by using 3C-SiC as IL. Regardingof electrical and optical characteristics, it is clearly observed that theLEDs with its IL have a smaller leakage current and higher light outputpower comparing with the LEDs without IL. The better performance ofLEDs using 3C-SiC IL can be contributed to both of the improvementsin epitaxial layers quality and light extraction efficiency. As aconsequence, in terms of optical property, a double enhancement of thelight output power and external quantum efficiency has been realized.

An Analyzing Method of Coupled Modes in Multi-Stage Planetary Gear System

Wei Sun,Jing Wei,Xin Ding,Xinglong Hu,Qingguo Wang 한국정밀공학회 2014 International Journal of Precision Engineering and Vol. No.

This paper analyzed the natural frequency and coupled mode characteristics in multi-stage planetary gear system. A dynamic modelof multi-stage planetary gears with general description is established based on the lumped mass method. Solving the associatedeigenvalue problem, the vibration modes of the system with identical, equally spaced planets are classified into rotational,translational and planet modes with unique properties. A criterion to distinguish the dominant vibration stage for the two coupledmode types, rotational mode and translational mode, are provided by comparing the eigensensitivity to the component parametersof different planetary stages. Taken the dominant vibration stage as an independent single-stage planetary gear system, the naturalfrequencies and vibration modes are observed to be similar to the multi-stage. It is shown that the coupling in the multi-stageplanetary gear system grows as the frequency reduces. In rotational modes and translational modes, the highest eigensensitivity tomesh stiffness and planet bearing stiffness of each stage are associated with different frequencies in the higher-frequency range, whilethe highest eigensensitivity to bearing stiffness of carriers, rings, and suns occurred in the lower-frequency range.

MRTF-A-NF-κB/p65 axis-mediated PDL1 transcription and expression contributes to immune evasion of non-small-cell lung cancer via TGF-β

Du Fu,Qi Xin,Zhang Aotong,Sui Fanfan,Wang Xuemin,Proud Christopher G.,Lin Cunzhi,Fan Xinglong,Li Jing 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

PD-L1 is abnormally regulated in many cancers and is critical for immune escape. Fully understanding the regulation of PD-L1 expression is vital for improving the clinical efficacy of relevant anticancer agents. TGF-β plays an important role in the low reactivity of PD-1/PD-L1 antibody immunotherapy. However, it is not very clear whether and how TGF-β affects PD-L1 expression. In the present study, we show that TGF-β upregulates the expression of the transcriptional coactivator MRTF-A in non-small-cell lung cancer cells, which subsequently interacts with NF-κB/p65 rather than SRF to facilitate the binding of NF-κB/p65 to the PDL1 promoter, thereby activating the transcription and expression of PD-L1. This leads to the immune escape of NSCLC cells. This process is dependent on the activation of the TGF-β signaling pathway. In vivo, inhibition of MRTF-A effectively suppresses the growth of lung tumor s y ngrafts with enrichment of NK and T cells in tumor tissue. Our study defines a new signaling pathway that regulates the transcription and expression of PD-L1 upon TGF-β treatment, which may have a significant impact on research into the application of immunotherapy in treating lung cancer.

Design and properties analysis of total internal reflection gratings for pulse compressor at 1053 nm

Qunyu Bi,Jiangjun Zheng,Ailin Guo,Meizhi Sun,Jianpeng Wang,Fuling Zhang,Qingwei Yang,Xinglong Xie,Zunqi Lin 한국물리학회 2011 Current Applied Physics Vol.11 No.1

High-efficiency compression gratings based on total internal reflection (TIR) are promising alternatives of compressor gratings because of their high diffraction efficiency, potential high damage resistant ability,and compact structure. Dependence of the 1 order diffraction efficiencies on grating parameters is analyzed for TE- and TM-polarized incident light of 1053 nm at Littrow angle, which is calculated by using the rigorous coupled-wave analysis. A more intuitional view on the relation is offered through three-dimensional slicing figures instead of two-dimensional ones. The performances of high-efficiency gratings are compared and regarded as criteria for further choices, including spectral bandwidth, angle bandwidth, dispersion, and intensity distribution. For TE- and TM-irradiations, similar spectral bandwidth and angle bandwidth can be achieved by different grating parameters. However, the computer simulation result on the intensity distributions of the two polarized waves shows that such design should be used under the illumination of TE-polarized wave for lower intensity enhancement ratio,which is an important factor related to the gratings’ damage threshold.