In situ atomic-scale observation of monolayer graphene growth from SiC

Yu, Kaihao,Zhao, Wen,Wu, Xing,Zhuang, Jianing,Hu, Xiaohui,Zhang, Qiubo,Sun, Jun,Xu, Tao,Chai, Yang,Ding, Feng,Sun, Litao Springer-Verlag 2018 NANO RESEARCH Vol.11 No.5

American ginseng significantly reduced the progression of high-fat-diet-enhanced colon carcinogenesis in Apc^Min/+ mice

Yu, Chunhao,Wen, Xiao-Dong,Zhang, Zhiyu,Zhang, Chun-Feng,Wu, Xiaohui,He, Xin,Liao, Yang,Wu, Ningning,Wang, Chong-Zhi,Du, Wei,He, Tong-Chuan,Yuan, Chun-Su The Korean Society of Ginseng 2015 Journal of Ginseng Research Vol.39 No.3

Background: Colorectal cancer (CRC) is a leading cause of death worldwide. Chronic gut inflammation is recognized as a risk factor for tumor development, including CRC. American ginseng is a very commonly used ginseng species in the West. Methods: A genetically engineered $Apc^{Min/+}$ mouse model was used in this study. We analyzed the saponin composition of American ginseng used in this project, and evaluated its effects on the progression of high-fat-diet-enhanced CRC carcinogenesis. Results: After oral ginseng administration (10-20 mg/kg/d for up to 32 wk), experimental data showed that, compared with the untreated mice, ginseng very significantly reduced tumor initiation and progression in both the small intestine (including the proximal end, middle end, and distal end) and the colon (all p < 0.01). This tumor number reduction was more obvious in those mice treated with a low dose of ginseng. The tumor multiplicity data were supported by body weight changes and gut tissue histology examinations. In addition, quantitative real-time polymerase chain reaction analysis showed that compared with the untreated group, ginseng very significantly reduced the gene expression of inflammatory cytokines, including interleukin-$1{\alpha}$ (IL-$1{\alpha}$), IL-$1{\beta}$, IL-6, tumor necrosis factor-${\alpha}$, granulocyte-colony stimulating factor, and granulocyte-macrophage colony-stimulating factor in both the small intestine and the colon (all p < 0.01). Conclusion: Further studies are needed to link our observed effects to the actions of the gut microbiome in converting the parent ginsenosides to bioactive ginseng metabolites. Our data suggest that American ginseng may have potential value in CRC chemoprevention.

Diffusion mechanism of deep shale gas and its carbon isotope fractionation: a combined simulated and mathematical analysis

Yu Zou,Guojian Wang,Cheng Tao,Rui Zhou,Shangqing Zhang,Dongna Liu,Xiaohui Lin,Xuying Zheng 한국지질과학협의회 2023 Geosciences Journal Vol.27 No.3

Unlike the release pressure process in shale gas exploitation, in this study, the concentration diffusion process is deeply discussed under constant temperature-pressure conditions, which aims to evaluate the preservation conditions of shale gas reservoirs in combination with isotopic fractionation characteristics. The isobaric diffusion experiment emphasized that decreasing pressure can lead to enhanced diffusion and isotope fractionation. The established mathematical model not only confirms the results of the simulating experiment, but also suggests that the contributions of Fick, Knudsen and surface diffusion to the migration of methane (including 12CH4 and 13CH4) vary in the geological evolution of shale reservoirs, which are mainly controlled by the pore system and gas pressure. Based on the analysis of specific samples of Longmaxi shale (r = 11.7 nm) and Niutitang shale (r = 1.3 nm), we propose that: 1) high pressure condition (> 20 MPa) can significantly limit Knudsen diffusion, which is the reason for reducing the total diffusion coefficient (DTotal) and isotope fractionation, while low pressure (< 10 MPa) and small pore radius (ca. 1 nm) can strengthen the fractionation; 2) pore connectivity and heterogeneity can lead to an order of magnitude change in DTotal, while the influence of temperature is relatively small. According to the mathematical model, it can be expected that enhanced dissipation intensity and isotope fractionation of shale gas may occur in the process of basin uplift and pressure relief, which can potentially be used for the evaluation of reservoir preservation.

Compressive Properties of High-distance Warp-knitted Spacer Flexible Composite

Xiaohui Zhang,Yuanyuan Yu,Andrews Boakye,Pibo Ma 한국섬유공학회 2018 Fibers and polymers Vol.19 No.5

This paper mainly introduces a high-distance warp-knitted spacer flexible composite material which is a special material consisting of high-distance warp-knitted spacer fabric and some coating materials. It introduces the designing process as well as the preparation of high-distance warp-knitted flexible composite material. In order to comprehend the influence of internal pressure on the material, the mechanical properties under 100 %, 80 % and 60 % gas pressure have been explored. A compression test under these three kinds of gas pressure was carried out and the related force displacement diagram, the stress-strain curve and the energy strain curve which directly reflects the variation of the mechanical properties of the composites material under the different gas pressure were obtained and presented. A deep investigation and analysis was conducted into knowing the compressive properties of the material and how they are affected by the gas pressure. The experimental results obtained shows that the compressive properties of the high-distance warp-knitted space flexible material is dependent on the amount of gas inserted. Again the compressive strength of the material keeps growing stronger with an increase in the intensity of the gas pressure.

Developing TCM clinical practice guidelines: A comparison between traditional Chinese medicine and western medicine

Xuan Yu,Shouyuan Wu,Juanjuan Zhang,Yue Hu,Meng Luo,Hongmei Zhao,Xuping Song,Yaolong Chen,Xiaohui Wang 한국한의학연구원 2023 Integrative Medicine Research Vol.12 No.2

Clinical practice guidelines in Traditional Chinese Medicine (CPG-TCM) is the recommendation that aims to provide the best service to users by identifying and summarizing the rules of prevention, diagnosis, treatment, rehabilitation, and regression of diseases based on systematic reviews of evidence and balancing the advantages and disadvantages of different interventions for clinical questions of Traditional Chinese Medicine. Over the past 30 years, the concept and methods of evidence-based medicine have had a significant impact on the development of clinical practice guidelines in Western Medicine (CPG-WM), and their standardized guideline development methods are being adapted and used in the development of CPG-TCM. However, the quality of CPG-TCM is far from CPG-WM, and the methodological system for developing CPG-TCM is not yet fully established. Therefore, this study aims to explore the methodological differences between CPG-TCM and CPG-WM and to inform the development of high-quality CPGTCM.

Downregulation of HuR Inhibits the Progression of Esophageal Cancer through Interleukin-18

Xu Xiaohui,Song Cheng,Chen Zhihua,Yu Chenxiao,Wang Yi,Tang Yiting,Luo Judong 대한암학회 2018 Cancer Research and Treatment Vol.50 No.1

Purpose The purpose of this study was to investigate the effect of human antigen R (HuR) downregulation and the potential target genes of HuR on the progression of esophageal squamous cell carcinoma (ESCC). Materials and Methods In this study, a proteomics assay was used to detect the expression of proteins after HuR downregulation, and a luciferase assay was used to detect the potential presence of a HuR binding site on the 3’-untranslated region (3'-UTR) of interleukin 18 (IL-18). In addition, colony formation assay, MTT, EdU incorporation assay, Western blot, flow cytometry, immunohistochemistry, transwell invasion assay, and wound healing assay were used. Results In the present study, we found that the expression of both HuR protein and mRNA levels were higher in tumor tissues than in the adjacent tissues. HuR downregulation significantly suppressed cell proliferation. In addition, the metastasis of esophageal cancer cells was inhibited, while the expression of E-cadherin was increased and the expression of matrix metalloproteinase (MMP) 2, MMP9, and vimentin was decreased after HuR knockdown. Moreover, silencing of HuR disturbed the cell cycle of ESCC cells mainly by inducing G1 arrest. Furthermore, proteomics analysis showed that downregulation of HuR in TE-1 cells resulted in 100 upregulated and 122 downregulated proteins, including IL-18 as a significantly upregulated protein. The expression of IL-18 was inversely regulated by HuR. IL-18 expression was decreased in ESCC tissues, and exogenous IL-18 significantly inhibited the proliferation and metastasis of ESCC cells. The 3'-UTR of IL-18 harbored a HuR binding site, as shown by an in vitro luciferase assay. Conclusion HuR plays an important role in the progression of esophageal carcinoma by targeting IL-18, which may be a potential therapeutic target for the treatment of ESCC.

American ginseng significantly reduced the progression of high-fatdiet-enhanced colon carcinogenesis in ApcMin/þmice

Chunhao Yu,Xiao-Dong Wen,Zhiyu Zhang,Chun-Feng Zhang,Xiaohui Wu,Xin He,Yang Liao,Ningning Wu,Chong-Zhi Wang,Wei Du,Tong-Chuan He,Chun-Su Yuan 고려인삼학회 2015 Journal of Ginseng Research Vol.39 No.3

Background: Colorectal cancer (CRC) is a leading cause of death worldwide. Chronic gut inflammation is recognized as a risk factor for tumor development, including CRC. American ginseng is a very commonly used ginseng species in the West. Methods: A genetically engineered ApcMin/þ mouse model was used in this study. We analyzed the saponin composition of American ginseng used in this project, and evaluated its effects on the progression of high-fat-diet-enhanced CRC carcinogenesis. Results: After oral ginseng administration (10e20 mg/kg/d for up to 32 wk), experimental data showed that, compared with the untreated mice, ginseng very significantly reduced tumor initiation and progression in both the small intestine (including the proximal end, middle end, and distal end) and the colon (all p < 0.01). This tumor number reduction was more obvious in those mice treated with a low dose of ginseng. The tumor multiplicity data were supported by body weight changes and gut tissue histology examinations. In addition, quantitative real-time polymerase chain reaction analysis showed that compared with the untreated group, ginseng very significantly reduced the gene expression of inflammatory cytokines, including interleukin-1a (IL-1a), IL-1b, IL-6, tumor necrosis factor-a, granulocyte-colony stimulating factor, and granulocyte-macrophage colony-stimulating factor in both the small intestine and the colon (all p < 0.01). Conclusion: Further studies are needed to link our observed effects to the actions of the gut microbiome in converting the parent ginsenosides to bioactive ginseng metabolites. Our data suggest that American ginseng may have potential value in CRC chemoprevention.

High mobility group box 3 promotes cervical cancer proliferation by regulating Wnt/β-catenin pathway

Shichao Zhuang,Xiaohui Yu,Ming Lu,Yujiao Li,Ning Ding,Yumei Ding 대한부인종양학회 2020 Journal of Gynecologic Oncology Vol.31 No.6

Objective: High mobility group box 3 (HMGB3) plays an important role in the developmentof various cancer. This study aims to explore whether HMGB3 regulates cervical cancer (CC)progression and elucidate the underlying mechanism. Methods: HMGB3 expression in clinical patients' tumor samples were determined byreal-time quantitative polymerase chain reaction (qRT-PCR) and western blot. HMGB3overexpression/knockdown were used to investigate its function. Cell apoptosis and cyclewere detected by Annexin V/PI staining and flow cytometry. In vivo tumor model was madeby subcutaneous injection of HeLa cells transfected with shRNAs targeting HMGB3 (sh HMGB31) into the flank area of nude mice. Western blot was used to detect the levels ofβ-catenin, c-Myc, and matrix metalloproteinase-7 (MMP-7) in Hela and CaSki cells transfectedwith sh-HMGB3 or shRNAs targeting β-catenin. Results: Both messenger RNA and protein levels of HMGB3 were upregulated in CC tissuesfrom patients. High expression level of HMGB3 had positive correlation with serosalinvasion, lymph metastasis, and tumor sizes in CC patient. Functional experiments showedthat HMGB3 could promote CC cell proliferation both in vitro and in vivo. The expressionlevels of c-Myc and MMP-7 were increased, resulting in regulating cell apoptosis, cell cycle,and activating Wnt/β-catenin pathway. Conclusions: Our data indicated that HMGB3 may serve as an oncoprotein. It could be used asa potential prognostic marker and represent a promising therapeutic strategy for CC treatment.

Transient-Performance-Oriented Discrete-Time Design of Resonant Controller for Three-Phase Grid-Connected Converters

Song, Zhanfeng,Yu, Yun,Wang, Yaqi,Ma, Xiaohui The Korean Institute of Power Electronics 2019 JOURNAL OF POWER ELECTRONICS Vol.19 No.4

The use of internal-model-based linear controller, such as resonant controller, is a well-established technique for the current control of grid-connected systems. Attractive properties for resonant controllers include their two-sequence tracking ability, the simple control structure, and the reduced computational burden. However, in the case of continuous-designed resonant controller, the transient performance is inevitably degraded at a low switching frequency. Moreover, available design methods for resonant controller is not able to realize the direct design of transient performances, and the anticipated transient performance is mainly achieved through trial and error. To address these problems, the zero-order-hold (ZOH) characteristic and inherent time delay in digital control systems are considered comprehensively in the design, and a corresponding hold-equivalent discrete model of the grid-connected converter is then established. The relationship between the placement of closed-loop poles and the corresponding transient performance is comprehensively investigated to realize the direct mapping relationship between the control gain and the transient response time. For the benefit of automatic tuning and real-time adaption, analytical expressions for controller gains are derived in detail using the required transient response time and system parameters. Simulation and experimental results demonstrate the validity of the proposed method.

The Effect of pH on the Efficiency of an SBR Processing Piggery Wastewater

LiLong Yan,Yu Liu,Yuan Ren,Xiaohui Wang,Haijing Liang,Ying Zhang 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.6

To treat piggery wastewater efficiently, thehydrolysis of urea (mainly derived from swine urine) inpiggery wastewater with the change of sewage pH must beconsidered. Using activated sludge, piggery wastewaterwas treated in a sequencing batch reactor (SBR), and theeffects of influent pH on SBR processing efficiency, sludgesettle ability, and sludge activity were investigated. Theresults showed that a high influent pH value contributed tothe improvement of the removal rate of ammonia nitrogenand reduction of the chemical oxygen demand (COD). When the influent pH was between 9.0 and 9.5, the removalrate of ammonia nitrogen was higher than 90%, and thereduction of COD from its original value was 80%. Theinfluent pH had a greater influence on sludge concentrationand sludge activity. When the influent pH increased from7.0 to 9.5, the sludge concentration increased from 2,350 to3,947 mg/L in the reactor, and the activities of ammoniumoxidizing bacteria (AOB) and nitrite oxidizing bacteria(NOB) first increased and then decreased. When theinfluent pH was 9.0 and 8.0, the maximum values (0.48 gO2/(g MLSS/day) and 0.080 g O2/(g MLSS/day)) werereached, and the sludge settling ratio was nearly steadybetween 20 and 35% in each reactor.