Apoptosis in response to heat stress is positively associated with heat-shock protein 90 expression in chicken myocardial cells in vitro

Xiao-Hui Zhang,Hong Wu,Shu Tang,Qiao-Ning Li,Jiao Xu,Miao Zhang,Ya-Nan Su,Bin Yin,Qi-Ling Zhao,Nicole Kemper,Joerg Hartung,Endong Bao 대한수의학회 2017 Journal of Veterinary Science Vol.18 No.2

To determine heat-shock protein (Hsp)90 expression is connected with cellular apoptotic response to heat stress and its mechanism, chicken (Gallus gallus) primary myocardial cells were treated with the Hsp90 promoter, aspirin, and its inhibitor, geldanamycin (GA), before heat stress. Cellular viability, heat-stressed apoptosis and reactive oxygen species level under different treatments were measured, and the expression of key proteins of the signaling pathway related to Hsp90 and their colocalization with Hsp90 were detected. The results showed that aspirin treatment increased the expression of protein kinase B (Akt), the signal transducer and activator of transcription (STAT)-3 and p-IKKa/b and the colocalization of Akt and STAT-3 with Hsp90 during heat stress, which was accompanied by improved viability and low apoptosis. GA significantly inhibited Akt expression and p-IKKa/b level, but not STAT-3 quantity, while the colocalization of Akt and STAT-3 with Hsp90 was weakened, followed by lower cell viability and higher apoptosis. Aspirin after GA treatment partially improved the stress response and apoptosis rate of tested cells caused by the recovery of Akt expression and colocalization, rather than the level of STAT-3 (including its co-localization with Hsp90) and p-IKKa/b. Therefore, Hsp90 expression has a positive effect on cellular capacity to resist heat-stressed injury and apoptosis. Moreover, inhibition of Hsp90 before stress partially attenuated its positive effects.

Numerical optimization of transmission bremsstrahlung target for intense pulsed electron beam

Xiao Yu,Jie Shen,Shijian Zhang,Jie Zhang,Nan Zhang,Ivan Sergeevich Egorov,Sha Yan,Chang Tan,Gennady Efimovich Remnev,Xiaoyun Le 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.2

The optimization of a transmission type bremsstrahlung conversion target was carried out with MonteCarlo code FLUKA for intense pulsed electron beams with electron energy of several hundred keV formaximum photon fluence. The photon emission intensity from electrons with energy ranging from300 keV to 1 MeV on tungsten, tantalum and molybdenum targets was calculated with varied targetthicknesses. The research revealed that higher target material element number and electron energy leadsto increased photon fluence. For a certain target material, the target thickness with maximum photonemission fluence exhibits a linear relationship with the electron energy. With certain electron energy andtarget material, the thickness of the target plays a dominant role in increasing the transmission photonintensity, with small target thickness the photon flux is largely restricted by low energy loss of electronsfor photon generation while thick targets may impose extra absorption for the generated photons. Thespatial distribution of bremsstrahlung photon density was analyzed and the optimal target thicknessesfor maximum bremsstrahlung photon fluence were derived versus electron energy on three targetmaterials for a quick determination of optimal target design

De novo assembly and characterization of antennal transcriptome reveal chemosensory system in Nysius ericae

Ya-Nan Zhang,Xiu-Yun Zhu,Qian Zhang,Cai-Yun Yin,Zhi-PingDong,Ling-Hua Zuo,Dao-Gui Deng,Liang Sun,Xiao-Ming Li 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.4

Chemosensory system plays important roles in insect behaviors, some key genes governing chemosensation can potentially be used as novel targets for pest control. Nysius ericae (Hemiptera: Lygaeidae) is a polyphagous insect pest found throughout Eurasia countries which causes damage to many important crops. Little is known about the chemosensory mechanisms of host location and mate choice in N. ericae at themolecular level. Here, we assembled the antennal transcriptome of N. ericae using Illumina sequencing technology. Analysis of the transcriptome data identified 148 candidate chemosensory genes, including 28 for odorant binding proteins (OBPs), 16 for chemosensory proteins (CSPs), 2 for sensory neuron membrane proteins (SNMPs), 83 odorant receptors (ORs), 12 ionotropic receptors (IRs), and 7 gustatory receptors (GRs). Our results greatly improve the gene inventory for N. ericae and provide valuable resources for analyzing the functions of some key genes. This information will aid in the development of better understanding of chemosensation in N. ericae, and indicate novel targets for managing its impact on agriculture. Chemosensory system plays important roles in insect behaviors, some key genes governing chemosensation can potentially be used as novel targets for pest control. Nysius ericae (Hemiptera: Lygaeidae) is a polyphagous insect pest found throughout Eurasia countries which causes damage to many important crops. Little is known about the chemosensory mechanisms of host location and mate choice in N. ericae at themolecular level. Here, we assembled the antennal transcriptome of N. ericae using Illumina sequencing technology. Analysis of the transcriptome data identified 148 candidate chemosensory genes, including 28 for odorant binding proteins (OBPs), 16 for chemosensory proteins (CSPs), 2 for sensory neuron membrane proteins (SNMPs), 83 odorant receptors (ORs), 12 ionotropic receptors (IRs), and 7 gustatory receptors (GRs). Our results greatly improve the gene inventory for N. ericae and provide valuable resources for analyzing the functions of some key genes. This information will aid in the development of better understanding of chemosensation in N. ericae, and indicate novel targets for managing its impact on agriculture.Chemosensory system plays important roles in insect behaviors, some key genes governing chemosensation can potentially be used as novel targets for pest control. Nysius ericae (Hemiptera: Lygaeidae) is a polyphagous insect pest found throughout Eurasia countries which causes damage to many important crops. Little is known about the chemosensory mechanisms of host location and mate choice in N. ericae at themolecular level. Here, we assembled the antennal transcriptome of N. ericae using Illumina sequencing technology. Analysis of the transcriptome data identified 148 candidate chemosensory genes, including 28 for odorant binding proteins (OBPs), 16 for chemosensory proteins (CSPs), 2 for sensory neuron membrane proteins (SNMPs), 83 odorant receptors (ORs), 12 ionotropic receptors (IRs), and 7 gustatory receptors (GRs). Our results greatly improve the gene inventory for N. ericae and provide valuable resources for analyzing the functions of some key genes. This information will aid in the development of better understanding of chemosensation in N. ericae, and indicate novel targets for managing its impact on agriculture.

Pu-erh Tea Powder Preventive Effects on Cisplatin-Induced Liver Oxidative Damage in Wistar Rats

Zheng, Xiao-Nan,Wang, Xiao-Wen,Li, Li-Ya,Xu, Zi-Wei,Huang, Hsin-Yi,Zhao, Jin-Sheng,Zhang, Duo,Yin, Xu,Sheng, Jun,Tang, Jin-Tian Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.17

Background: Chemotherapy is one of the major means for control of malignancies, with cisplatin (CDDP) as one of the main agents, widely used for the treatment of various malignant solid tumors. However, prevention of hepatotoxicity from cisplatin is one of the urgent issues in cancer chemotherapy. In this study, we aimed to investigate the effects of pu-erh tea on hepatotoxicity through body weight and tissue antioxidant parameters like, liver coefficient, serum alanine aminotransferase (ALT), serum aspartate aminotransferase (AST), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), malondialdehyde(MDA) and glutathione (GSH) levels, and light microscopic evaluation by histological findings. Materials and Methods: The rats were randomly divided into five groups: Control (n=10), cisplatin (3 mg/kg p.i., n=10), cisplatin+pu-erh (0.32 g/kg/day i.g., n=10), cisplatin+pu-erh (0.8 g/kg/day i.g., n=10) and cisplatin+pu-erh (1.6 g/kg/day i.g., n=10). Pu-erh tea powder was administrated for 31 consecutive days. The rats were sacrificed at the end on the second day after a single dose of cisplatin treatment for measuring indices. Results: Pu-erh tea powder exhibited a protective effect by decreasing MDA and GSH and increasing the SOD and GSH-PX levels and GSH-PX/MDA ratio in camparison with the control group. Besides, pu-erh tea was also able to alleviate the pathological damage to some extent. Conclusion: Pu-erh tea powder is protective against cisplatin-induced liver oxidative damages, especially at the medium dosage (0.8 g/kg/d).

Development of a BDDE-crosslinked hyaluronic acid based microneedles patch as a dermal filler for anti-ageing treatment

Jia Nan Zhang,Bo Zhi Chen,Mohammad Ashfaq,Xiao Peng Zhang,Xin Dong Guo 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.65 No.-

Hyaluronic acid (HA) has many applications in human medicine and cosmetic industry. This study describes the fabrication and evaluation of cross-linked HA (cHA) based microneedles (HA-cHA-MNs) for anti-ageing treatment. The results suggested that the increasing content of cHA gel particles decrease the mechanical properties of MNs. The in-vitro degradation profile and in-vivo epidermal expansion in mice were also carried out. The data suggested that the proportion of cHA particles decreased the rate of MNs degradation. The prepared HA-cHA-MNs have prolonged effectiveness with high swelling retention time or epidermal expansion time in mice up to 6 days or more.

Posttranscriptional deregulation of Src due to aberrant miR34a and miR203 contributes to gastric cancer development

Qiang Hao,Xiao Zhao Lu,Nan Nan Liu,Xiao Chang Xue,Meng Li,Cun Zhang,Xin Qin,Wei Na Li,Zhen Shu,Bin Song,Qing Wang,Li Qiang Song,Wei Zhang,Ying Qi Zhang 생화학분자생물학회(구 한국생화학분자생물학회) 2013 BMB Reports Vol.46 No.6

THREE-DIMENSIONAL PARAMETRICMODELING OF CHEMICAL PLANT EQUIPMENT BASED ON THE UNIT MODEL

Nan-Li,Yan-Zhang,Xiao chu-Tang 한국멀티미디어학회 2006 한국멀티미디어학회 국제학술대회 Vol.2006 No.-

Secondary development of Pro/Engineer is studied using the parametric principle based on unit model after analyzing the structure of tube sheet in order to complete three-dimensional parametric modeling of chemical plant equipment in this article. Meanwhile, this paper takes the heat exchanger as the example to introduce the process.

Gene-metabolite network analysis in different nonalcoholic fatty liver disease phenotypes

Xiao-Lin Liu,Ya-Nan Ming,Jing-Yi Zhang,Xiao-Yu Chen,Min-De Zeng,Yi-Min Mao 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-

We sought to identify common key regulators and build a gene-metabolite network in different nonalcoholic fatty liver disease (NAFLD) phenotypes. We used a high-fat diet (HFD), a methionine-choline-deficient diet (MCDD) and streptozocin (STZ) to establish nonalcoholic fatty liver (NAFL), nonalcoholic steatohepatitis (NASH) and NAFL+type 2 diabetes mellitus (T2DM) in rat models, respectively. Transcriptomics and metabolomics analyses were performed in rat livers and serum. A functional network-based regulation model was constructed using Cytoscape with information derived from transcriptomics and metabolomics. The results revealed that 96 genes, 17 liver metabolites and 4 serum metabolites consistently changed in different NAFLD phenotypes (42-fold, Po0.05). Gene-metabolite network analysis identified ccl2 and jun as hubs with the largest connections to other genes, which were mainly involved in tumor necrosis factor, P53, nuclear factor-kappa B, chemokine, peroxisome proliferator activated receptor and Toll-like receptor signaling pathways. The specifically regulated genes and metabolites in different NAFLD phenotypes constructed their own networks, which were mainly involved in the lipid and fatty acid metabolism in HFD models, the inflammatory and immune response in MCDD models, and the AMPK signaling pathway and response to insulin in HFD+STZ models. Our study identified networks showing the general and specific characteristics in different NAFLD phenotypes, complementing the genetic and metabolic features in NAFLD with hepatic and extra-hepatic manifestations.

Modulation of the Self-Assembly of Collagen by Phytic Acid: An In Vitro Study

Xiao Tu,Xincheng Chen,Ying Peng,Jie Nan,Benmei Wei,Lang He,Chengzhi Xu,Yuling Xu,Dong Xie,Juntao Zhang,Haibo Wang 한국고분자학회 2018 Macromolecular Research Vol.26 No.13

Phytic acid, containing a myoinositol ring coupled with six phosphate groups, can react with the amino groups of collagen to regulate their self-assembly behavior. The aim of this research is to evaluate the effects of phytic acid on the selfassembly behavior of collagen, the structures and properties of the resulting fibrils and hydrogels. Turbidity and chloramine T assay suggested that phytic acid could improve the self-assembly kinetics and degree of collagen, and the optimal ratio of phytic acid/collagen was 1/1 (w/w). Scanning electron microscopy (SEM) analysis indicated that co-fibrils of collagen with phytic acid are more slender than that of pure collagen, and transmission electron microscopy (TEM) reveals that the characteristic D-periodicity of collagen fibrils is not affected by phytic acid. Besides, differential scanning calorimetry (DSC) and rheology revealed that the thermal stability of collagen fibrils and the viscoelasticity of collagen hydrogels could be improved by phytic acid and the optimal ratio of phytic acid/collagen is 1/1 (w/w).

Impact of Caspase-8 (CASP8) -652 6N Del and D302H Polymorphisms on Prostate Cancer in Different Ethnic Groups

Zhang, Cheng-Dong,Li, Hong-Tao,Liu, Kun,Lin, Zhi-Di,Peng, Qi-Liu,Qin, Xue,He, Min,Wu, Hua,Mo, Zeng-Nan,Yang, Xiao-Li Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.18

Background: Despite evidence suggesting roles for caspase-8 (CASP8) -652 6N del and D302H polymorphisms in prostate cancer (PCa), the association of these polymorphisms with PCa risk remains inconclusive. Therefore, a meta-analysis was performed to more precisely estimate the association of CASP8 -652 6N del and D302H polymorphisms with PCa susceptibility. Materials and Methods: A comprehensive literature search was conducted to identify all case-control studies of CASP8 D302H and -652 6N del polymorphisms and PCa risk. Odds ratios (ORs) and 95% confidence intervals (CIs) were used to assess the strength of the association and the precision of the estimate, respectively. Results: Nine -625 6N del studies and 4 D302H studies were included. CASP8 -652 6N del and D302H polymorphisms were not significantly associated with PCa risk in the overall analyses. However, in the subgroup analysis stratified by ethnicity, -625 6N del was significantly associated with PCa risk in the East Asian and Indian populations under the recessive model. Furthermore, the subgroup analysis strongly suggested that D302H was associated with lower PCa risk in the Non-Indian population under the dominant model. Conclusions: In our meta-analysis, ethnic-specific differences were evident in the association of CASP8-625 6N del and D302H polymorphisms with PCa risk.