이종 유전자에 의한 형질전환된 담배의 광합성 능력회복

우제창 木浦大學校基礎科學硏究所 1996 基礎科學硏究誌 Vol.14 No.-

완두의 rbcS 유전자가 삽입된 pAC1350 vector 에 hygromycin transfere 유전자를 선별지표 (selector marker) 로 재삽입하여 p35SSU-HPH vector을 구성하고 이를 이미 antisence 유전자에 RUBISCO small subunit 유전자 발현이 억제된 식물체에 형질전환시켜 재분화를 유도하고 계속적으로 배양하여 그 종자를 채종하였다. 채종된 종자는 발아시 hygromycin이 함유된 배지에서도 16% 정도는 생장이 가능하였고, Southern 과 Northern bolt hybridization 의 결과 HPH 유전자를 핵내에 보유하고 있으며 완두의 rbcS유전자의 전사도 이루어지는 것으로 확인되었다. 이들을 생장속도에 따라 대, 중, 소로 구분하여 Southern bolt hybridization을 수행한 결과 모식물체 (parental plant)에서 보호하고 있는 4개 이상의 antisence 유전자는 0 - 4개의 band로 검출되어 생장이 좋을수록 antisence 유전자의 copy 수가 적은 것으로 확인되었다. 이들 종자의 광합성 능력은 모식물체와 정상 담배 식물체(SRI)의 중간을 나타내어 완두의 rbcS 유전자가 종자를 통하여도 전달됨을 보였고 이들 식물체의 세포내 CO₂ 농도는 400 μbar 정도로 일정하게 유지되어 삽입된 유전자에 의해 외부로부터 CO₂를 흡수하는 능력이 향상된 것으로 나타났다. RUBISO (Ribulose bisphosphate carboxylase/oxygenase) is sharply reducd in transgenic tobacco carrying and rbcS antisense(Rodermel et al., Cell 55:673-681/ 1988):expression of most if all member of the tobacoo rbcS family is reduced. To replace the tobacco rbcS gene products, plants with the tobacco antisense gene were transformed with T-DNA containing a 35S cauliflower mosaic virus promoter : pea rbcS gene and the hygromycin phosphotransferase gene. Such transgenic plants accumulated more RUBISCO small subunit protein than the parental strain and their photosynthetic activity was enhanced up to 150%. Progenies of selfed "double- transgenic" plants showed similar, though variable, patterns of hygromycin resistance and enhanced photosynthetic activity.

Rayleigh파의 해석을 통한 다층토의 동적 물성 추정에 관한 연구

우제윤,이두희,김수일 연세대학교 산업기술연구소 1985 논문집 Vol.17 No.2

In this study, a data reduction method is developed to predict the dynamic shear modulus of each layer of multi-layered soil from the measured surface wave velocities. The method developed utilizes the vertical amplitude attenuation curve of surface wave in homogeneous soil. Numerical model studies show that the technique developed in this study is more accurate compared with the one-third technique widely used for the time being. The maximum error of data reduction from the technique developed in this study is found to be approximately one half of that of data reduction from the one-third technique. It is also found in this study that assumed Poisson`s ratio may by used without causing any significant error, however, unit weight of each layer should be exactly estimated in order not to cause any significant error.

항정신병약물을 처음으로 사용하는 초발 정신증 환자에서 나타나는 체중 및 대사성 지표의 변화 : 후향적 연구

김우진,심주철,공보금,강제욱,문정준,김정은,김민걸,박민경,김성진,김현정,정도운 대한생물치료정신의학회 2011 생물치료정신의학 Vol.17 No.2

Objectives:This study was to identify weight & metabolic changes in first-episode psychotic patients with antipsychotics use and investigate the differences of weight & metabolic changes between first-episode psychotic patients and controls with antipsychotics use. Method:In this retrospective study, twenty eight first-episode psychotic patients and twenty eight controls with schizophrenia, schizoaffective or bipolar disorder defined by DSM-IV criteria were included. Information about demographic and clinical characteristics of the subjects was gathered from the medical records. Also body weight, sBP, dBP, fasting glucose, lipid profile and WBC count were evaluated before and after antipsychotics use. Rogistic regression was conducted to assess factors associated with weight gain. Results:First-episode psychotic patients showed more weight and BMI changes than controls after antipsychotics use, and these changes continue over 12 months. On the other hand, there were no significant factors associated with weight gain. Conclusion:The results of present study suggest that antispychotics is one of the major causes inducing weight gain of psychotic patients and antipsychotics-induced weight gain is more vulnerable to drug-naive first-episode psychotic patients.

1945년 이후의 태국문학의 발전 : 삶을 위한 문학을 중심으로 Centering around Literature for Life

우제혁 한국외국어대학교 동남아연구소 1994 東南亞硏究 Vol.- No.3

Cray T3E 상에서의 선형 시스템 해를 위한 COIS 프로그램

우제학 대진대학교 정보통신연구소 1998 정보통신 논문집 Vol.1 No.-

Recently, challenges to larger and more complex problems have been pursued in several scientific as well as commercial fields. The progrmamers in these fields have turned to high-performance parallel computing in order to find solutions for their problems in ever shorter times, However, parallel programming is a very difficult job because it needs a different concept with traditional sequential programming, In this paper, we present parallel programming using an implicit parallel language, COIS. We especially focus in solutions for linear systems, because they are usually generated in process of solving partial differential equation in scientfic computation. We consider three methods solving linear systems, and present COIS progrmas based on the algotithms.

Chlamydomonas reinhardtii 엽록체 DNA의 형질전환

우제창 木浦大學校 基礎科學硏究所 1992 基礎科學硏究誌 Vol.10 No.-

pCrc44-rbcL-AAD, consist of rbcL promotor and aminoglycoside adenyl transferase (aadA) structural gene in atpB region of Chlamydomonas reinhardtii chloroplast DNA, was transformed to Chlamydomonas reinhardtii chloroplast by microprojectile bombardment. 37 clonies were selected as photosynthesis competent cell line on HS minimal medium, among them, 12 clonies showed spectinomycin resistant as a result of expression of aadA. All transformed cell line showed two distinctivly hybridized bands,4.1kb and 3.1kb, by using 0.5kb atpB fragment for probe. It meant that all of them were heterokaryon of normal and aadA transformed cells.

공진 기초를 이용한 아스팔트 콘크리트의 동적 전단계수 추정에 관한 연구

우제윤,김수일,이형인 연세대학교 산업기술연구소 1985 논문집 Vol.17 No.2

In this study, a resonant footing technique to estimate the dynamic shear modulus of asphalt concrete using the torsional vibration test is developed. From this study an empirical formula is proposed to estimate the dynamic shear modulus of asphalt concrete. The numerical results are compared with the values estimated from the measurement of primary wave velocities. The oscillator made for this study has thin circular bars which are considered as springs. Heavy upper part of the oscillator is connected to lower aluminum plate by means of springs. The oscillator is designed so that torsional vibration of aluminum plate is caused by magnetic force and footing-asphalt concrete system also vibrates together with aluminum plate. From the experimental result, the maximum difference of the dynamic shear modulus from the resonant footing test and primary wave velosity detection shows 3% deviation when the Poisson`s ratio is assumed to be 0.3. The apparatus made for this study is thought to estimate the dynamic shear modulus of asphalt concrete properly. It is also found that the dynamic shear modulus is not influenced by confining pressure within the range of confining pressure applied in this study.

한국인 젊은 여성의 심미적인 안면 연조직 형태에 관한 두부 X-선 계측학적 연구

우제경,성재현,권오원 대한치과교정학회 1997 대한치과교정학회지 Vol.27 No.2

본 연구에서는 대구에서 활동하고 있는 모델과 심미적인 안모를 가졌다고 추천받은 68명의 젊은 여성을 대상으로 하여 두부X-선 사진, 정모 사진과 측모 실루엣 사진을 채득하였다. 두부X-선 사진의 연조직 측모선만을 사용한 3명씩의 측모 실루엣을 FH plane이 평행하게 한 장에 그려서 제작한 사진을 경북대학교 미술학과 재학생 7명과 교정 전공의 15명에게 평가하게 하였으며 정모 사진을 교정 전공의만 평가하게 하였다. 아주 좋은 경우를 9점, 좋은 경우를 7점 보통인 경우를 5점, 좋지 않은 경우를 3점으로 처리토록 하였다. 측모 실루엣에서 교정 전공의와 미대생의 심미안간의 상관관계, 교정 전공의가 평가한 정모와 측모의 심미성간의 상관관계, 교정 전공의가 평가한 측모 실루엣의 평균 점수가 5점 이상인 38명의 측모 계측항목과 측모 심미성간의 상관관계를 분석하여 다음과 같은 결론을 얻었다. 1. 측모 실루엣에서 교정 전공의와 미대생의 심미안간의 상관관계는 유의성있게 나타났다(r=0.67, P=0.0001). 2. 교정 전공의가 평가한 정모와 측모의 심미성간의 상관관계는 유의성있게 나타났다(r=0.26, P=0.0381). 3. 측모 실루엣과 상관관계가 유의성있게 나타난 항목은 Na-Pog to N′, BNV to Pog′, BNV/B′-Pog′, Ls-Li-Pog′, Li-B′-Pog′, Z angle이었다.(P<0.05). 4. 측모 계측항목의 평균치 및 표준편차를 구하였다. Cephalometric radiographs, frontal photographs and profile silhiuette phogographs of 68 young adult female who were model or were recommended to have esthetic face were used in this study. 7 Students in department of Art of Kyungpook national university and 15 orthodontists estimated profile slides which were made of 3 profile silhouettes in parallel with FH plane. Profile silhouettes were made of soft tissue profile line of cephalometric radiograph. Only orthodontists estimated frontal photographs. Students and Orthodontists score 9 in excellent case, score 7 in good case, score 5 in average case, score 3 in poor case. Correlation analysis between orthodontists` esthetic concept and Artists` esthetic concept, between frontal view esthetics and profile view esthetics which estimated by orthotontists. between profile view esthetics and profile measurements which consisted of measurements of 38 female who were scored above 5 mean score in profile silhouette by orthodontists were done. And the finding in this study indicated the following 1. Correlation between orthodontists` esthetic concept and Artists` esthetic concept in profile silhouette was significant(r=0.67, P=0.0001). 2. Correlation between frontal view esthetics and profile view esthetics which estimated by orthodontist was significant(r=0.26, P=0.0381). 3. Measurements which had significant correlation between profile measurements and profile view esthetics were Na-Pog to N′, BNV to Pog′, BNV/B′-Pog′, Ls-Li-Pog′, Li-B′-Pog′and Z angle(P<0.05). 4. Mean and standard deviation of profile measurements of 38 female were obtained.

한국인 젊은 여성의 심미적인 안면 연조직 형태에 관한 두부 X-선 계측학적 연구

우제경 慶北大學校 齒科大學 1995 慶北齒大論文集 Vol.12 No.1

대구에서 활동하고 있는 모델과 심미적인 안모를 가졌다고 추천받은 68명의 젊은 여성을 대상으로 하여 두부X-선 사진, 정모 사진과 측모 실루엣 사진을 채득하였다. 경북대학교 미술학과 재학생 7명과 교정 전공의 15명에게 두부X-선 사진의 연조직 측모선만을 사용한 3명씩의 측모 실루엣을 FH plane이 평행하게 한장에 그려서 제작한 사진을 평가하게 하였으며 정모 사진은 교정 전공의만 평가하게 하였다. 아주 좋은 경우를 9점, 좋은 경우를 7점, 보통인 경우를 5점, 좋지 않은 경우를 3점으로, 처리토록 하였다. 측모 실루엣에서 교정 전공의와 미대생의 심미안간에 상관관계, 교정 전공의가 평가한 정모와 측모의 심미성간에 상관관계, 교정 전공의가 평가한 측모 실루엣의 평균 점수가 5점 이상인 38명의 측모 계측항목과 측모 심미성간의 상관관계를 분석하여 다음과 같은 결론을 얻었다. 1. 측모 실루엣에서 교정 전공의와 미대생의 심미안간에 상관관계는 유의성있게 나타났다(r=0.67,P=0.0001) 2. 교정 전공의가 평가한 정모와 측모의 심미성간에 상관관계는 유의성있게 나타났다(r=0.26,P=0.0381). 3. 측모 실루엣과 상관관계가 유의성있게 나타난 항목은 Na-Pog to N',BNV to Pog', BNV/B'-Pog', Ls-Li-Pog', Li-B'-Pog', Z angle이었다 (P<0.05). 4. 측모 계측항목의 평균치 및 표준편차를 구하였다. Cephalometric radiographs, frontal photographs and profile silhouette phogographs of 68 young adult female who were model or were recommended to have esthetic face were used in this study. 7 Students in department of Art of Kyungpook national university and 15 orthodontists estimated profile slides which were made of 3 profile silhouettes in parallel with FH plane. profile silhouttes were made of soft tissue profile line of cephalometri radiograph. Only orthodontists estimated frontal photographs. Students and Orthodontists score 9 in excellent case, score 7 in good case, score 5 in average case, score 3 in poor case. Correlation analysis between orthodontists' esthetic concept and Artists; esthetic concept, between frontal view esthetics and profile view esthetics which estimated by orthorontists, between profile view esthetics and profile measurement which consisted of measurements of 38 female who were scored above 5 mean score in profile silhouette by orthodontists were done. And the finding in this study indicated the following 1. Correlation between orthodontists' esthetic concept and Artists' esthetic concept inprofile silhouetre was significant(r=0.67,P=0.0001). 2. Correlation between frontal view esthetics and profile view esthetics which estimated by orthodontist was significant.(r=0.26,P=0.0381). 3. Measurements which had significant correlation between profile measurements and profile view esthetics were Na-Pog to N', BNV to Pog', BNV/B'-Pog', Ls-Li-Pog', Li-B'-Pog' and Z angle (P<0.05). 4. Mean and standard deviation of profile measurements of 38 female were obtained.

MMPI를 이용한 악교정 수술환자의 인성에 관한 연구

박우경,박재억,박수병 대한치과교정학회 1998 대한치과교정학회지 Vol.28 No.5

이 연구에서는 악교정 수술환자의 술전, 술후 인서의 특성 및 술후 변화를 알아보기 위해 실시되었으며 객관적인 인성검사의 한가지인 MMPI를 이용하였다. 악교정 수술환자들의 수술동기와 술후 만족도는 질문지를 통하여 조사하였다. 연구대상은 술전교정 중인 환자 50명과 악교정 수술을 받은 환자 22명으로 이루어졌다. 이 연구에서 얻은 결과는 다음과 같다. 1. 술전 환자군에서 K 척도를 제외한 모든 T-scores는 정상적인 범주에 놓여 있었다. K 척도값은 57.7이었으며 이것은 이 환자들이 다소 스트레스를 많이 받고 있음을 보여주었다. 2. 술후 환자군은 모든 척도에서 정상범주에 놓여 있었다. 3. 술전, 술후 환자군을 비교했을 때, 단지, T-score만이 수술 후에 통계학적으로 유의성있는 증가를 보였다.(P<0.05) 4. 남성환자는 술전 K 척도를 제외한 모든 척도에서 정상적이었으며, 술후에 K, Hs 척도에서 통계학적으로 유의성있는 증가를 보였다.(P<0.05) 5. 여성환자는 모든 T-score기 정상적인 범주에 놓여 있었다. 6. 수술동기는 크게 심미적, 기능적, 악관절문제, 정신사회학적 동기로 나눌 수 있었으며, 각각 28.2, 32.1, 9.0, 30.7%였다. 7. 수술 후 수술 동기에 대한 만족도는 매우 만족하는 환자가 전체의 14.3%, 만족하는 환자가 71.4%, 그저그렇다고 답한 환자가 9.5%였으며, 매우 불만족스러움을 답한 환자가 4.8%였다. This study was designed to evaluated the personality characteristics of orthognathic surgery patients preoperatively and postoperatively through the MMPI. Comparison was made between the personality characteristics of preoperative and postoperative groups. Motivation for undergoing orthognatic surgery and postoperative satisfaction were examined trough the questionnaire. Subjects used in this study consisted 50 patients under presurgical orthodontic treatment and 22 patients who had received orthognathic surgery. From this study, the results were followings. 1. In preoperative patients group, all T-scores were within the normal range except for K-scale. The T-score of K-scale is 57.7, this showed that these patients underwent some of less stress. 2. In postoperative patients group, all T-scores were within the normal range. 3. Only in K-scale, there was statistically significant increase after surgery(P<0.05). 4. In male patients group, all T-scores were within the normal range except for that of preoperative K-scale. Postoperatively, there were statistically significant increase in K and Hs scales(P<0.05). 5. In female patients group, all T-scores were within the normal range. 6. Motivation for undergoing orthognathic surgery could be divided into 4 categories.:esthetic goal(28.2%), functional goal(32.1%), TMJ problem(9.0%), and psychosocial reason(30.7%). 7. The degree of postoperative satisfaction was quite high(85.7%). 14.3% of all patients were very satisfied, 71.4% were satisfied, 9.5% were neither satisfied or dissatisfied, and 4.8% were very dissatisfied.