Catecholamine-storing Cells at Acupuncture Points of Rabbits

소광섭,Vyacheslav Ogay,Min Su Kim,Hyo Jun Seok,Cheon Joo Choi, 사단법인약침학회 2008 Journal of Acupuncture & Meridian Studies Vol.1 No.2

Recent studies have shown that specific sites of the skin related to the acu points contain a high concentration of catecholamines, especially noradrenaline (NA). Considering this newly discovered property of the acupoints we assumed that heterogeneous distribution of cutaneous catecholamines could be associated with a specific location of catecholamine-storing cells in acupoint sites. In the present work we used an immunohistochemical method and confocal laser scanning microscopy to examine the presence of catecholamine-storing cells at acupoints of rabbits. Double immunofluorescence staining with antibodies against adrenaline and NA revealed only the cells storing NA in the dermal layer of rabbit skin. NA-storing cells were randomly scattered as single cells as well as existing in small clusters in a globular tissue formation surrounded by blood vessels and capillaries. Microscopic analysis of histological sections also revealed that the distribution of NA-storing cells was closely associated with the location of acupoints. Thus results from our study strongly suggest that acupoint areas of rabbit skin contain catecholamine-storing cells which can release a high level of NA during acupuncture stimulation.

Catecholamine-Producing Novel Endocrine Organ: Bonghan System

Kim, Jungdae,Ogay, Vyacheslav,Lee, Byung-Cheon,Kim, Min-Su,Lim, Inbin,Woo, Hee-Jong,Park, Hi-Joon,Kehr, Jan,Soh, Kwang-Sup Mary Ann Liebert 2008 Medical acupuncture Vol.20 No.2

Acupuncture Muscle Channel in the Subcutaneous Layer of Rat Skin

Kwang-Sup Soh,Byung-Cheon Lee,Vyacheslav Ogay,Yuwon Lee,Jin-Kyu Lee,Ki Woo Kim 사단법인약침학회 2008 Journal of Acupuncture & Meridian Studies Vol.1 No.1

Using a mixed-dye injection technique, we found a novel kind of muscle fiber with a lumen, established its precise location in the subcutaneous muscle layer along the acupuncture muscle of the bladder line, and determined its detailed ultrastructure. The channels with flowing liquid were a novel kind of muscle fibers with lumens and they were located in the subcutaneous muscle layer of rat. Their detection was realized by using chrome-hematoxylin and a mixture of fluorescent nanoparticles and commercial Pelikan ink. These acupuncture muscle channels were hidden among the neighboring skin skeletal muscle fibers and were barely distinguishable from them with light microscopes. Only with a transmission electron microscope were their characteristic features shown to be different from normal skin skeletal muscle. These features included undifferentiated muscle fibers that resembled immature myofibrils without Z-lines and reassembled telophase nuclei. Using a mixed-dye injection technique, we found a novel kind of muscle fiber with a lumen, established its precise location in the subcutaneous muscle layer along the acupuncture muscle of the bladder line, and determined its detailed ultrastructure. The channels with flowing liquid were a novel kind of muscle fibers with lumens and they were located in the subcutaneous muscle layer of rat. Their detection was realized by using chrome-hematoxylin and a mixture of fluorescent nanoparticles and commercial Pelikan ink. These acupuncture muscle channels were hidden among the neighboring skin skeletal muscle fibers and were barely distinguishable from them with light microscopes. Only with a transmission electron microscope were their characteristic features shown to be different from normal skin skeletal muscle. These features included undifferentiated muscle fibers that resembled immature myofibrils without Z-lines and reassembled telophase nuclei.

Electron microscopic study of novel threadlike structures on the surfaces of mammalian organs

Lee, Byung-Cheon,Yoo, Jung Sun,Ogay, Vyacheslav,Kim, Ki Woo,Dobberstein, Harald,Soh, Kwang-Sup,Chang, Byung-Soo Wiley Subscription Services, Inc., A Wiley Company 2007 Microscopy research and technique Vol.70 No.1

<P>The ultrastructures of novel threadlike structures (NTSs) and corpuscles on the surfaces of internal organs of rats were investigated using electron microscopy. The samples were studied in situ by using a stereomicroscope and were taken for further morphological analysis. Scanning electron microscope (SEM) images revealed a bundle structure of threadlike tissue, which was composed of several 10-μm-thick subducts. The surfaces of the corpuscles were rather coarse and fenestrated. The corpuscles had cucumber-like shapes with an average length of about 2 mm and a thickness of about 400 μm. Transmission electron microscope (TEM) images disclosed disordered collagen fibers, which formed the extracellular matrix of the threadlike tissue, and immune-function cells, like macrophages, mast cells, and eosinophils. Sinuses of various diameters, which were thought to be cross-sections of the lumens of the subducts, were observed in the TEM, cryo-SEM and focused-ion-beam SEM images. These SEM images were obtained for the first time to reveal the detailed structure of the NTSs that were only recently discovered. Microsc. Res. Tech., 2006. © 2006 Wiley-Liss, Inc.</P>

Intradermal Alcian-Blue Injection Method to Trace Acupuncture Meridians

성백경,김민수,오가이,강대인,소광섭,Sung, Baeck-Kyoung,Kim, Min-Su,Ogay, Vyacheslav,Kang, Dae-In,Soh, Kwang-Sup KOREAN PHARMACOPUNCTURE INSTITUTE 2008 Journal of pharmacopuncture Vol.11 No.2

목적 : 본 논문에서는 경락을 추적하기 위한 피내(皮內) 알시안 블루(Alcian blue) 염색 방법을 기술한다. 방법 : 1% 알시안 블루 용액을 31게이지 바늘이 달린 0.5mL 인슐린 주사기를 사용하여 경혈 지점에 피내 주사한 후 실체 현미경하에서 수술, 관찰하였다. 면역조직화학적 방법을 사용하여 해당 조직을 레이저 공초점주사현미경으로 관찰하였다. 결과 : 알시안 블루로 염색되고 피하로 이어지는 실 모양 구조물을 관찰하였다. 이 조직 내에서 특징적인 막대모양 핵 배열 및 $1-2{\mu}m$크기의 데옥시리보핵산 입자가 관찰되었다. 또한 경혈 조직 내에서 풍부한 모세혈관총 및 말초신경 말단, 그리고 약 $300{\mu}m$크기의 소체형 구조물을 확인하였다. 결론 : 경혈 지점의 피내에서 발견된 특징적 실 모양 구조물 및 소체형 구조물은 각각 표층 봉한관 및 소체로 판단된다. Objective In this article, we report on the intradermal Alcian blue staining method for tracing the meridians of acupuncture. Methods 1% Alcian blue solution was injected into acupoints by using a 0.5mL insulin syringe with a 31-gauge needle, then the skin was incised and was observed under a stereoscopic microscope. The specimens were examined by using immunohistochemical methods and were observed under a confocal laser scanning microscope. Results A threadlike structure, which was visualized with Alcian blue, existed in dermis layer and proceeded to hypodermis. In this structure, characteristic alignments of rod- shaped nuclei and $1-2{\mu}m$ sized DNA granules were observed. Furthermore, abundant blood capillary plexuses, peripheral nerve endings, and a corpuscle-like structure(about $300{\mu}m$ in diameter) were visualized in the skin tissues of acupoints. Conclusion It was concluded that the specific threadlike and corpuscle-like structures corresponded to superficial Bonghan duct and corpuscle, respectively.

Novel circulatory connection from the acupoint Zhong Wan(CV12) to pancreas

김민수,성백경,최천주,김민수,강대인,소광섭,Kim, Min-Soo,Sung, Baeck-Kyoung,Ogay, Vyacheslav,Choi, Chun-Ju,Kim, Min-Su,Kang, Dae-In,Soh, Kwang-Sup KOREAN PHARMACOPUNCTURE INSTITUTE 2008 Journal of pharmacopuncture Vol.11 No.1

Objectives : Demonstrating a novel circulatory path from the acupoint(CV12) to the pancreas. Method : Alcian blue(1% solution, $20{\mu}l$, pH 7.4) was injected into the acupoint(CV12). Two hours later the surfaces of internal organs were observed by using a stereomicroscope. Results : Alcian blue arrived and colored the omental fat band(OFB) on the pancreas. The OFB connected the head and tail of the pancreas, the pancreas and the spleen, and the pancreas and the stomach. Conclusion : The existence of a novel circulatory path from the acupoint CV12 to the pancreas and its OFB was demonstrated. 목적 : 피부의 경혈(CV12)로부터 췌장으로 액체가 흘러가는 경로가 있음을 보임. 방법 : 생쥐의 중완혈에 푸른색 염료(Alcian blue) 1% 용액을 주입하고, 2시간이 경과한 후 개복하여 내장장기의 표면들을 실체 현미경으로 관찰한다. 결과 : 췌장 위에 있으며, 췌장의 머리부분과 꼬리부분, 췌장과 위장, 췌장과 비장을 연결해주는 망막상의 지방줄(omental fat band)에 Alcian blue가 도달, 착색되었다. 결론 : 중완혈로부터 췌장을 경과하여 망막상의 지방줄에 이르는 액체 순환경로가 있다.

Primo-Vessels as New Flow Paths for Intratesticular Injected Dye in Rats

한현정,Jin-Kyu Lee,Yu Won Lee,Ki-Woo Kim,Byung-Cheon Lee,박상준,Vyacheslav Ogay,소광섭 사단법인약침학회 2010 Journal of Acupuncture & Meridian Studies Vol.3 No.2

After intratesticular injection of a chromium hematoxylin and fluorescent nanoparticle solution, we found a novel flow path in the abdominal cavity consisting of primo-vessels and primo-nodes. This flow path formed a network that crossed over the surfaces of abdominal organs, and generally linked to the greater omentum and adjacent visceral peritoneum. Some of these structures terminated at organs such as the small intestine and the urinary bladder; occasionally, the network entered the parenchyma of organs. The semitransparent primo-vessels and nodes were wholly or partially stained dark-blue by chromium hematoxylin. Injected nanoparticles were also observed in primo-vessels and nodes as well as the parenchyma of organs which were the site of primo-vessel termination. Transmission electron microscopy showed that the primo-vessels consist of many sinuses (4−6 μm), surrounded by collagen fibers, specific granules, cellular remnants, immune cells,extracellular matrices, and hematopoietic cells. These histological features are completely different from blood and lymph vessels indicating that primo-vessels are novel structures that allow the flow of dye.

Measurement of the Optical Properties of In-Vitro Organ-Surface Bonghan Corpuscles of Rats

Yong-Hui Han,Joon-Mo Yang,Byung-Cheon Lee,유정선,김정대,Ku-Youn Baik,Kwang-Sup Soh,Min-Su Kim,Sang-Hyun Park,Vyacheslav Ogay 한국물리학회 2006 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.49 No.III