Stilbenes and Oligostilbenes from Leaf and Stem of Vitis amurensis and Their Cytotoxic Activity

Do Thi Ha,Quan Cheng Chen,Tran Manh Hung,Tran Minh Ngoc,Phuong Thien Thuong,김홍진,성연희,민병선,배기환,윤의중 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.2

Chromatographic separation of the EtOAc fraction from the leaf and stem of Vitis amurensis led to the isolation of six oligostilbenoids (i.e., r-2-viniferin (1), trans-amurensin B (2), trans-ε -viniferin (3), gnetinH (4), amurensin G (5), (+)-ampelopsin A (8)) and four stilbenoids (i.e., trans-resveratrol (6), (+)- ampelopsin F (7), piceatannol (9), and trans-piceid (10)). The structures have been identified on the basis of spectroscopic evidence and physicochemical properties. The isolates were investigated for cytotoxic activity against three cancer cell lines in vitro using the MTT assay method. Amurensin G (5) and trans-resveratrol (6) showed significant cytotoxic activity against L1210, K562 and HTC116 cancer cell lines with IC50 values ranging from 15.7 ± 2.1 to 30.9 ± 1.8 μM. (+)-Ampelopsin A (8) and trans-piceid (10) exhibited considerable cytotoxic activity against L1210 (IC50 values of 30.6 ± 4.1 and 28.7 ± 2.81μM, respectively) and K562 (IC50 values of 38.6 ± 0.82 and 24.6 ± 0.76 μM, respectively). Gnetin H (4)showed only weak cytotoxic activity against L1210 with an IC50 value of 40.1 ± 4.23 μM. On the other hand, r-2-viniverin (1), trans-amurensin B (2), trans-ε -viniferin (3), (+)-ampelopsin F (7), and piceatannol(9) exhibited no activity on three cancer cell lines.

Determinants of Intra-Industry Trade between Vietnam and Countries in TPP

NGUYEN, Ha Minh,QUAN, Binh Quoc Minh,LE, Huong Van,TRAN, Thinh Van Korea Distribution Science Association 2020 The Journal of Asian Finance, Economics and Busine Vol.7 No.1

Intra-industry trade (IIT) has played an important role in international trade of Vietnam as a result of rapid growth of the country. This article investigates the level of IIT between Vietnam and 11 trading partners in Trans-Pacific Partnership (TPP) over the period 2000-2014. Although there have been a large number of empirical researches contributed to the determinants of IIT, most of them only pay attention on developed countries where the trade flows are similar due to similar demand structure and production technology. Until now, there is no study on intra-industry trade between Vietnam and countries in TPP that Vietnam recently signed a trade agreement in early 2016. IIT is measured by the Grubel-Lloyd index. The index shows that the extent of intra-industry trade between Vietnam and the trading countries is not high due to the level of economic development and the market size. The determinants of IIT are examined using a panel regression model. In the empirical analysis, the results indicate that Vietnam's intra-industry trade is positively correlated with country size, while it is negatively correlated with income dissimilarity, the trade openness, and geographical distance. This study contributes to the new theoretical trade theory on the evidence of developing country's IIT.

Suppression of lung inflammation by the methanol extract of <i>Spilanthes acmella</i> Murray is related to differential regulation of NF-κB and Nrf2

Kim, Kyun Ha,Kim, Eun Jung,Kwun, Min Jung,Lee, Ji Yeon,Bach, Tran The,Eum, Sang Mi,Choi, Jun Yong,Cho, Sayeon,Kim, Sang-Jun,Jeong, Seung-Il,Joo, Myungsoo Elsevier 2018 Journal of Ethnopharmacology Vol.217 No.-

<P><B>Abstract</B></P> <P><B>Ethnopharmacological relevance</B></P> <P>Although <I>Spilanthes acmella</I> has been used to relieve inflammation, fever, pain, or infection in traditional Asian medicine, experimental evidence supporting these functions is scarce. Here, we examined an anti-inflammatory function and a possible underlying mechanism of <I>S. acmella</I> Murray (SAM).</P> <P><B>Materials and method</B></P> <P>The methanol extract of SAM was fingerprinted by HPLC. C57BL/6 mice were administered with a single intratracheal (i.t.) LPS and 2 h later with a single i.t. SAM. The effect of SAM on lung inflammation was assessed by histology, semi-quantitative RT-PCR, and MPO assay of lung tissue. The effects of SAM on a pro-inflammatory factor NF-κB and an anti-inflammatory factor Nrf2 were analyzed by immunoblotting of nuclear proteins and by semi-quantitative RT-PCR analysis of mRNA of the genes governed by these transcription factors. V5-Nrf2 was precipitated by an anti-V5 antibody and the ubiquitinated V5-Nrf2 was revealed by immunoblotting of HA-tagged ubiquitin.</P> <P><B>Results</B></P> <P>The i.t. SAM robustly diminished a neutrophilic lung inflammation induced by i.t. LPS treatment of mice. In RAW 264.7 cells, SAM suppressed the nuclear localization of NF-κB and the expression of NF-κB-dependent cytokine genes. SAM increased the level of Nrf2 in the nucleus and the expression of Nrf2-dependent genes while suppressing ubiquitination of Nrf2.</P> <P><B>Conclusion</B></P> <P>Our results suggest that SAM can suppress a neutrophilic inflammation in mouse lungs, which is associated with suppressed NF-κB and activated Nrf2. Our results provide experimental evidence supporting the anti-inflammatory function of <I>S. acmella</I>.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Identification of Soluble Epoxide Hydrolase Inhibitors from the Seeds of Passiflora edulis Cultivated in Vietnam

To Dao Cuong,Hoang Thi Ngoc Anh,Tran Thu Huong,Pham Ngoc Khanh,Vu Thi Ha,Tran Manh Hung,김영호,Nguyen Manh Cuong 한국생약학회 2019 Natural Product Sciences Vol.25 No.4

Soluble epoxide hydrolases (sEH) are enzymes present in all living organisms, metabolize epoxy fatty acids to 1,2-diols. sEH in the metabolism of polyunsaturated fatty acids plays a key role in inflammation. In addition, the endogenous lipid mediators in cardiovascular disease are also broken down to diols by the action of sEH that enhanced cardiovascular protection. In this study, sEH inhibitory guided fractionation led to the isolation of five phenolic compounds trans-resveratrol (1), trans-piceatannol (2), sulfuretin (3), (+)-balanophonin (4), and cassigarol E (5) from the ethanol extract of the seeds of Passiflora edulis Sims cultivated in Vietnam. The chemical structures of isolated compounds were determined by the interpretation of NMR spectral data, mass spectra, and comparison with data from the literature. The soluble epoxide hydrolase (sEH) inhibitory activity of isolated compounds was evaluated. Among them, trans-piceatannol (2) showed the most potent inhibitory activity on sEH with an IC50 value of 3.4 mM. This study marks the first time that sulfuretin (3) was isolated from Passiflora edulis as well as (+)-balanophonin (4), and cassigarol E (5) were isolated from Passiflora genus.

The Impact of Global Uncertainty Shocks on Macroeconomics: The Case of Vietnam

Ha Hong TRAN(Ha Hong TRAN ),Vinh Thi Hong NGUYEN(Vinh Thi Hong NGUYEN ),Nam Hoang TRINH(Nam Hoang TRINH ) 한국유통과학회 2022 The Journal of Asian Finance, Economics and Busine Vol.9 No.9

The global financial crisis of 2008–2009 and the COVID-19 pandemic that started in 2019 along with the slow and unstable recovery of the global economy have raised concerns about the impact of global uncertainty on the macroeconomics of the countries. The paper used the Structural Vector Autoregression (SVAR) model to examine the impact of global uncertainty shocks on Vietnam’s economy from the period 2008–2022. We found that Vietnam’s output dropped following the shock of global uncertainty, the peak was in the third month, and lasted for one year. Inflation in Vietnam had a rapid downturn in the first month, peaked in the seventh month, and took a long time to cease. When the economy experienced the shock of increased global uncertainty, Vietnam’s policy interest rate was adjusted downward. Additionally, we included a long-term interest rate to consider the overall impact of monetary policy into account. A decreasing trend was also found with this rate. The global uncertainty shock effects acted as the aggregate demand shocks, reducing output and inflation as the uncertainty increases and vice versa, thus monetary policy can be used to regulate Vietnam’s economy to deal with negative shocks without the trade-offs between output and inflation as aggregate supply shocks.

Isolation and Cytotoxic Potency of Endophytic Fungi Associated with Dysosma difformis, a Study for the Novel Resources of Podophyllotoxin

Hoa Thi Tran,Giang Thu Nguyen,Hong Ha Thi Nguyen,Huyen Thi Tran,Quang Hong Tran,Quang Ho Tran,Ngoc Thi Ninh,Phat Tien Do,Ha Hoang Chu,Ngoc Bich Pham 한국균학회 2022 Mycobiology Vol.50 No.5

Endophytic fungi are promising sources for the production of podophyllotoxin-an important anticancer compound, replacing depleted medical plants. In this study, the endophytes asso- ciated with Dysosma difformis-an ethnomedicinal plant species were isolated to explore novel sources of podophyllotoxin. Fifty-three endophytic fungi were isolated and identified by morphological observation and ITS-based rDNA sequencing, assigning them to 27 genera in 3 divisions. Fusarium was found the most prevalent genus with a colonization frequency of 11.11%, followed by Trametes (9.26%) and Penicillium (7.41%). Phylogenetic trees were constructed for the endophytic fungi community in two collection sites, Ha Giang and Lai Chau, revealing the adaptation of the species to the specific tissues and habitats. Cytotoxic activity of endophytic fungal extracts was investigated on cancer cell lines such as SK-LU-1, HL-60, and HepG2, demonstrating strong anti-cancer activity of six isolates belonging to Penicillium, Trametes, Purpureocillium, Aspergillus, and Ganoderma with IC50 value of lower than 10 mg/mL. The presence of podophyllotoxin was indicated in Penicillium, Trametes, Aspergillus and for the first time in Purpureocillium and Ganoderma via high-performance liquid chromatography, which implied them as a potential source of this anti- cancer compound.

Medicinal Chemistry : Inhibition of Human Low Density Lipoprotein and High Density Lipoprotein Oxidation by Oligostilbenes from Rhubarb

( Tran Minh Ngoc ),( Tran Manh Hung ),( Phuong Thien Thuong ),( Min Kyun Na ),( Hong Jin Kim ),( Do Thi Ha ),( Byung Sun Min ),( Pham Thi Hong Minh ),( Ki Hwan Bae ) 영남대학교 약품개발연구소 2009 영남대학교 약품개발연구소 연구업적집 Vol.19 No.-

Development of multifunctional self-cleaning and UV blocking cotton fabric with modification of photoactive ZnO coating via microwave method

Tran Thi, Viet Ha,Lee, Byeong-Kyu Elsevier Sequoia 2017 Journal of photochemistry and photobiology Chemist Vol. No.

<P><B>Abstract</B></P> <P>A new self-cleaning cotton fabric coated with zinc oxide (ZnO) was obtained via a facile and effective microwave method. The solution pH was a key factor in determining the morphology of ZnO crystal growth on the cotton fibers: rod structures were apt to be formed at pH 4–5, whereas flake and flower-like structures were preferentially formed at higher solution pHs of 6–7 and 10–11, respectively. It is proved that the photocatalytic and wettability performances of ZnO could be efficiently transferred to cotton fabric, leading to a preeminent UV-blocking and self-cleaning cotton fabric. The identified UV protection factors (UPFs) indicate the excellent UV-blocking properties of the ZnO-coated textile fabric synthesized at pH 6–7, 8–9 and 10–11 with UPFs of 222.52, 162.68 and 202.57, respectively. The ZnO coating layer on the cotton fabric significantly improved the wetting behavior of the fabric, such as water absorption speed, thereby affording a self-cleaning ability for the ZnO-coated cotton fabrics. This ability was checked according to the removal degree of coffee stain under UV irradiation at different humidities (30–90% RH). The coffee stains on the ZnO-coated cotton fabrics were almost removed after 15h without needing any water or detergent and the highest removal effect was observed at 90% humidity.</P> <P><B>Highlights</B></P> <P> <UL> <LI> ZnO-coated cotton fabric materials were fabricated by rapid microwave method. </LI> <LI> The structures of the ZnO crystals varied according to the seeding solution pH. </LI> <LI> The ZnO-coated cotton fabric showed excellent UV protection property (UPF 50+). </LI> <LI> The wetting behavior of the fabric surfaces was affected by the ZnO coating layer. </LI> <LI> Self-cleaning activity was verified by the removal of coffee stains under UV light. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Automated MRI Brain Extraction Using K-means Clustering and Anisotropic Diffusion Filter

Tran Thi Thu Ha,Jin Young Kim,Seungho Choi 한국정보기술학회 2013 한국정보기술학회논문지 Vol.11 No.12

In this paper, we propose a method to extract brain region from magnetic resonance imaging (MRI). The technique is commonly applied to assess brain atrophy acquired by taking MRI. The collected information is essential monitoring disease progress. Therefore it plays an important role in developing a computer-aided diagnosis of vascular dementia. For brain atrophy measurement, main regions, which should be focused and extracted to calculate an atrophy ratio from MRI, are white matter (WM) and gray matter (GM) and region containing cerebrospinal fluid (CSF). The proposed method helps us segment WM and GM regions from T2-weighted MRI. It consists of three main steps of normalizing pre-processing, clustering and post-processing. The first pre-processing step standardizes and enhances the brightness of brain region using complement image and Otsu’s method. The second step is clustering process using anisotropic diffusion filter and k-means clustering to separate brain region from background and non-bran region. Finally the post-processing step refines brain region extracted from the previous step using morphology technique. The experimental results on real T2-weighted MRI database demonstrate that our proposed approach has a good performance with high accuracy of 95.24%.

Methods for Studying on Morphological Characteristic of Asian Honey Bee (Apis cerana)

Tran Van Toan,Myeong Lyeol Lee,Ha Sik Sim,Hye Kyung Kim,Gyu Ho Byuon,Yong Soo Choi 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.10

Asian is rich in honey bee species and genetic diversity. Among the difference native honey bee species, Apis cerana is very diversity of subspecies and distribution as well. Until now, nine A. cerana subspecies have been named. However, natural diversity of this species is being declined by threats such as pest, disease, deforestation, pesticide positioning and climate change. Therefore, the understanding of morphological characteristics of A. cerana is viral for maintaining biological diversity. In this paper we give an overview of method that are used for distinguish honey bee A. cerana subspecies and ecotype that can contribute to recognize genetic origin of colonies for conservation and breeding purpose. Base on morphmetric method currently in use, we outline strategies for sampling and measuring morphological characteristics on A. cerana.