Flavihumibacter profundi sp. nov., isolated from eutrophic freshwater sediment

Ting-Ting Ren,Chunzhi Jin,Feng-Jie Jin,Taihua Li,김창진,오희목,이형관,Long Jin 한국미생물학회 2018 The journal of microbiology Vol.56 No.7

A Gram-stain-positive, aerobic, non-motile, non-spore-forming, and rod-shaped bacterium, designated strain CHu64- 6-1T, was isolated from a 67-cm-long sediment core collected from the Daechung Reservoir at a water depth of 17-m in Daejeon, Republic of Korea. Comparative 16S rRNA gene sequence studies placed the new isolate in the class Sphingobacteriia, and the isolate is notably most closely related to Flavihumibacter sediminis CJ663T (98.1% similarity), Flavihumibacter solisilvae 3-3T (97.8%), Flavihumibacter petaseus T41T (97.5%), Flavihumibacter cheonanensis WS16T (97.4%), and Flavihumibacter stibioxidans YS-17T (97.2%). The cells of strain CHu64-6-1T formed yellow colonies on R2A agar and contained MK-7 as the only menaquinone, phosphatidylethanolamine, an unidentified phospholipid, and two unidentified aminolipids as the major polar lipids, and C15:0 iso, C17:0 iso 3-OH, C15:1 iso G, and C16:1 ω5c as the major fatty acids (> 5%). The DNA G + C content of the genome was determined to be 46.5 mol%. The DNA-DNA hybridization values of strain CHu64-6-1T with F. sediminis CJ663T, F. solisilvae 3-3T, F. petaseus T41T, F. cheonanensis WS16T, and F. stibioxidans YS-17T were 12.4–33.2%. Based on the combined genotypic and phenotypic data, we propose that strain CHu64-6-1T represents a novel species of the genus Flavihumibacter, for which the name Flavihumibacter profundi sp. nov. is proposed. The type strain is CHu64-6-1T (= KCTC 62290T = CCTCC AB 2018060T).

Prognostic Value of PLCE1 Expression in Upper Gastrointestinal Cancer: a Systematic Review and Meta-analysis

Cui, Xiao-Bin,Peng, Hao,Li, Su,Li, Ting-Ting,Liu, Chun-Xia,Zhang, Shu-Mao,Jin, Ting-Ting,Hu, Jian-Ming,Jiang, Jin-Fang,Liang, Wei-Hua,Li, Na,Li, Li,Chen, Yun-Zhao,Li, Feng Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.22

Background: A number of studies have identified a shared susceptibility locus in phospholipase C epsilon 1 (PLCE1) for esophageal squamous cell carcinoma (ESCC) and gastric cardia adenocarcinomas (GCA). However, the results of PLCE1 expression in esophageal and gastric cancer remain inconsistent and controversial. Moreover, the effects on clinicopathological features remain undetermined. This study aimed to provide a precise quantification of the association between PLCE1 expression and the risk of ESCC and GCA through meta-analysis. Materials and Methods: Eligible studies were identified from PubMed, Wanfang Data, ISI Web of Science, and the Chinese National Knowledge Infrastructure databases. Using RevMan5.2 software, pooled odds ratios (ORs) with 95% confidence intervals (CIs) were employed to assess the association of PLCE1 expression with clinicopathological features relative to ESCC or GCA. Results: Seven articles were identified, including 761 esophageal and gastric cancer cases and 457 controls. Overall, we determined that PLCE1 expression was associated with tumor progression in both esophageal cancers (pooled OR=5.93; 95%CI=3.86 to 9.11) and gastric cancers (pooled OR=9.73; 95%CI=6.46 to 14.7). Moreover, invasion depth (pooled OR=3.62; 95%CI=2.30 to 5.70) and lymph node metastasis (pooled OR=4.21; 95%CI=2.69 to 6.59) were linked with PLCE1 expression in gastric cancer. However, no significant associations were determined between PLCE1 overexpression and the histologic grade, invasion depth, and lymph node metastasis in esophageal cancer. Conclusions: Our metaanalysis results indicated that upregulated PLCE1 is significantly associated with an increased risk of tumor progression in ESCC and GCA. Therefore, PLCE1 expression can be appropriately regarded as a promising biomarker for ESCC and GCA patients.

Structure and Thermal-enhanced Magnetic Properties of Mn4C Melt-spun Ribbons with Varied Stoichiometry

Ting-Ting Qi,Ping-Zhan Si,Fang Cheng,Zhi-Rui Wang,Hong-Liang Ge,Qiong Wu,Jihoon Park,Chul-Jin Choi 한국자기학회 2023 Journal of Magnetics Vol.28 No.1

Cubic perovskite-type Mn4C is difficult to prepare for its metastable characteristics. In this work, we have obtained high-purity Mn4C successfully by using melt-spinning method. The effects of stoichiometry on the structure and magnetic properties of the samples were studied systematically. We found that x = -0.1 is the optimum composition for the formation of the cubic perovskite phase in Mn4+xC during rapid quenching. Most Mn4+xC melt-spun ribbons with x other than -0.1 are composed of Mn23C6, α-Mn, and Mn4C, while the fraction of different phase in Mn4+xC ribbons varies with x. The Curie temperature of Mn4+xC ribbons increases slightly with decreasing x, which may affect the lattice parameters of cubic Mn4C and thus the Mn-Mn exchange interactions. The magnetization of Mn4+xC (x = -0.1 and 0) increases with increasing temperature in high-temperature region while the onset temperature for such behavior is dependent on the fraction of Mn4C in the samples.

Developmental Hypothyroidism Influences the Development of the Entorhinal-Dentate Gyrus Pathway of Rat Offspring

Ting Jin,Ranran Wang,Shiqiao Peng,Xin Liu,Hanyi Zhang,Xue He,Weiping Teng,Xiaochun Teng 대한내분비학회 2022 Endocrinology and metabolism Vol.37 No.2

Background: Developmental hypothyroidism impairs learning and memory in offspring, which depend on extensive neuronal circuits in the entorhinal cortex, together with the hippocampus and neocortex. The entorhinal-dentate gyrus pathway is the main entrance of memory circuits. We investigated whether developmental hypothyroidism impaired the morphological development of theentorhinal-dentate gyrus pathway. Methods: We examined the structure and function of the entorhinal-dentate gyrus pathway in response to developmental hypothyroidism induced using 2-mercapto-1-methylimidazole. Results: 1,1´-Dioctadecyl-3,3,3´,3´-tetramethylindocarbocyanine perchlorate tract tracing indicated that entorhinal axons showeddelayed growth in reaching the outer molecular layer of the dentate gyrus at postnatal days 2 and 4 in hypothyroid conditions. Theproportion of fibers in the outer molecular layer was significantly smaller in the hypothyroid group than in the euthyroid group atpostnatal day 4. At postnatal day 10, the pathway showed a layer-specific distribution in the outer molecular layer, similar to the euthyroid group. However, the projected area of entorhinal axons was smaller in the hypothyroid group than in the euthyroid group. Anelectrophysiological examination showed that hypothyroidism impaired the long-term potentiation of the perforant and the cornuammonis 3–cornu ammonis 1 pathways. Many repulsive axon guidance molecules were involved in the formation of the entorhinaldentate gyrus pathway. The hypothyroid group had higher levels of erythropoietin-producing hepatocyte ligand A3 and semaphorin3A than the euthyroid group. Conclusion: We demonstrated that developmental hypothyroidism might influence the development of the entorhinal-dentate gyruspathway, contributing to impaired long-term potentiation. These findings improve our understanding of neural mechanisms formemory function.

S100A4 Expression is Closely Linked to Genesis and Progression of Glioma by Regulating Proliferation, Apoptosis, Migration and Invasion

Jin, Ting,Zhang, Zhuo,Yang, Xue-Feng,Luo, Jun-Sheng Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.7

Background: The calcium-binding S100A4 protein is involved in epithelial to mesenchymal transition, oncogenic transformation, angiogenesis, cytoskeletal integrity, mobility and metastasis of cancer cells. This study aimed to clarify the roles of S100A4 in genesis and progression of glioma. Materials and Methods: S100A4 expression was examined by real-time RT-CPR and Western blot in glioma and paired normal brain tissue (n=69), and compared with clinicopathological parameters of tumors. In addition, glioma U251 cells transfected with an S100A4-expressing plasmid were examined for proliferation by MTT, apoptosis by Annexin V-FITC, and migration and invasion with Transwell chambers. Results: Increased S100A4 mRNA expression was found in gliomas, compared with paired non-tumor tissue (p<0.001). Gradual elevation of overexpression of S100A4 was observed with increasing glioma grade (p<0.001). Astrocytoma showed lower S100A4 mRNA expression than oligodendrogliomas, with glioblastomas having highest values (p<0.001). Similar results were obtained for S100A4 protein, a positive link being found between mRNA and protein expression in gliomas (p<0.001). There was higher growth, lower apoptosis, stronger migration and invasion of S100A4 transfectants than control and mock transfected cells (p<0.001). Conclusions: These findings indicate that up-regulated S100A4 expression is positively linked to pathogenesis, progression and histogenesis of glioma by modulating proliferation, apoptosis, migration and invasion.

Transcriptome analysis of entomopathogenic fungus Beauveria bassiana-infected bean bug (Riptortus pedestris)

Se Jin Lee,Yi-Ting Yang,Yu-Shin Nai,Jae Su Kim 한국응용곤충학회 2016 한국응용곤충학회 학술대회논문집 Vol.2016 No.04

Beauveria bassiana (Bb) is an entomopathogenic fungus with a wide host range, and is commonly used as an environment-friendly biopesticide. However, the molecular mechanisms of Bb-host interactions are not well understood. Here, RNA isolated from a highly virulent strain of B. bassiana (Bb JEF-007) and Riptortus pedestris (Hemiptera: Alydidae) (bean bug) infected with this strain were subjected to high throughput next generation sequencing (NGS) to analyze and compare transcriptomes. Differentially expressed gene (DEG) analysis showed that 2,381 genes were up-regulated and 2,303 genes were down-regulated upon infection. Most DEGs were classified into the categories of single-organism, cellular and metabolism processes by gene ontology (GO) analysis. Carbon metabolism-related enzymes in the glyoxylate cycle were significantly up-regulated, suggesting a possible role for them in Bb growth in the host. This work provides insight into how entomopathogenic B. bassiana occupies agriculturally harmful bean bug at the late stage, which might be essential during fungal infection.

민국시기 연변지역에 있어서의 통역양성학교 - 설립경위를 중심으로 -

김정실 ( Jin Ting Shi ) 한국일어일문학회 2020 日語日文學硏究 Vol.114 No.-

연변의 지리적 위치와 정치적 상황의 특성으로 이 지역을 둘러싸고 역사적으로 다양한 역학적인 움직임이 있었다. 본 연구에서는 연변조선족자치주 당안관에 소장된 역사자료를 활용하여 외국어 야반(夜班) 연구소와 한국역재소(譯才所)에 대한 분석을 통해 그 실태를 조사하여 객관적 역사사실에 접근하였다. 민 국(民國)측은 1914년 11 월에 통역사를 양성하는 외국어야반(夜班)연구소를 설립하고 일본어, 러시아어, 한국어를 가르쳤으며 1916 년에는 한국어역재소(譯才所)를 설립하여 조선어, 국문, 일본어를 가르쳤고 졸업생들은 연길현기관(機關) 에 등록되었다. 이와같이 민국(民國)측은 당시 복잡한 정치지리적상황으로 보다 적극적으로 통역사 양성학교를 설립하여 국내 정치안정을 도모하려고 했다는 점을 본 연구를 통하여 규명하였다. The geographical location and political situation made a great significance to Yanbian region. In this study, I tried to explore the actual condition by analyzing the history of the Evening School of Foreign Language and the Korean Translation Center, consulting the files of the Archives of Yanbian, Korean Autonomous Prefecture. In november 1914, the Republic of China established the Evening School of Foreign Language, which trained interpreters, and taught Japanese, Russian and Korean. In 1916, the Republic of China established the Korean Translation Center, which taught Korean, Chinese and Japanese, and graduates worked in the institutions of the prefectures of Yanjidao. Thus, this study revealed that the Republic of China actively established the school of interpretation at that time to achieve the political stability.

Transcriptome analysis of entomopathogenic fungus Beauveria bassiana-infected bean bug (Riptortus pedestris) to identify possible fungal virulence factors

Se Jin Lee,Yi-Ting Yang,Yu-Shin Nai,Jae Su Kim 한국응용곤충학회 2015 한국응용곤충학회 학술대회논문집 Vol.2015 No.10

The ascomycete fungus Beauveria bassiana is a wide host range entomo- pathogenic fungus, which is commonly used as an environmental friendly biopesticide. However, the molecular mechanisms of host-pathogen interaction of B. bassiana are not well understood. Here, the high throughput next generation sequencing was performed to analyze the transcriptome of B. bassiana JEF-007 infected bean bug (Riptorus pedestris). Differentially expressed gene (DEG) analysis results showed that total 4,684 genes including 2,381 up and 2,303 down regulated genes were identified. Most of the DEGs were classified into single- organism, cellular and metabolism processes by gene ontology (GO) analysis. Metabolism pathway was the most abound category of DEGs via KEGG pathway mapping. Several possible candidates of virulence factors were dramatically expressed after infection, such as cytotoxic lectin, bacterial-like toxin, and proteins related to cell wall, hyphal growth, nutrient uptake and halogenated compounds synthesis. Furthermore, we also found the highest expression of a novel small RNA virus in the infected bean bug, but the relationship between fungal virulence and the RNA virus was under determination. The functional roles of these possible virulence factors are remained unclear, but this work provides a new insight for further fungal studies. Our results reflect systemic impacts of fungal pathogenesis and these findings represent a significant advance in the fungal functional genomics.

Protective effects of vitamins C and E on dichlorodiphenyltrichloroethane-induced genotoxicity and hepatotoxicity in human liver cells

Xiao Ting Jin,Li Song,Xiang Yuan Liu,Hang Qing Li,Long Cheng,Zhuo Yu Li1,,Z. Y. Li 대한독성 유전단백체 학회 2017 Molecular & cellular toxicology Vol.13 No.2

Dichlorodiphenoxytrichloroethane (DDT), a persistent organic pollutant and hepatotoxicant, is used to control the malaria. However, scarce information exists on relevant effective inhibitors of DDT’ toxicity. The aim of this study was to investigate protective effects of natural antioxidants vitamin C (VC) or/and vitamin E (VE) on p,pʹ-DDT-induced genotoxicity and hepatotoxicity in human liver cells. p,pʹ-DDT exposure increased levels of chromatin condensation, comet parameter, micronucleus induction and DPC coefficient of HL-7702 cells in a dose-dependent manner. Also, an increase in mRNA levels of CYP1A1 as well as CYP3A4, and a decrease in UGT along with GST were observed. Interestingly, supplementation with VC or/and VE prevented p,pʹ-DDT-caused alterations in DNA damage and phase metabolism gene expressions, and the combination of VC and VE had a more protective effect than VC or VE alone. These findings illustrated that VC or/and VE could be beneficial for the alleviation of p,pʹ-DDT-incurred toxicity in human liver cells.

A Novel Mannose-binding Tuber Lectin from Typhonium divaricatum (L.) Decne (family Araceae) with Antiviral Activity Against HSV-II and Anti-proliferative Effect on Human Cancer Cell Lines

( Yong Ting Luo ),( Xiao Chao Xu ),( Ji Wei Liu ),( Jian Li ),( Yi Sheng Sun ),( Zhen Liu ),( Jin Zhi Liu ),( Els Van Damme ),( Jan Balzarini ),( Jin Ku Bao ) 생화학분자생물학회 2007 BMB Reports Vol.40 No.3