筋小胞體의 ATPase Kinetics에 關한 硏究

朴映淳,李承兩,朴泰水,庾基洙,李 弘,金允坤 圓光大學校 1982 論文集 Vol.16 No.2

본 연구에서는 토끼 골격근 소포체에서 분리한 근소포체의 ATPase활성에 대한 일반적 특성과 수종의 2가 양이온(??)의 영향에 관한 연구를 하였다. ATPase활성과 Ca uptake에 미치는 2가 양이온의 저해 효과와 저해기작은 Dixon plot, Lineweaver-Burk plot, Hanes-Woolf plot, Woolf-Augustinsson-Hofstee plot, Eadie-Scatchard plot, Arrhenius plot등을 이용하여 분석하였으며 그 결과는 다음과 같다. 1. 근소포체의 ATPase는 ??과 ??의 존재하에서 활성화 되어지며 ??의 농도가 4mM일 때 ??의 최적 농도는 0.1mM이었다. 2. 근소포체의 ATPase는 ??-ATPase와 (??)-ATPase로 구성되어 있고 (??) - ATPase의 활성은 ??-ATPase의 활성보다 높았다. 3. ATPase의 활성화 에너지는 약 19Kcal/mol이었다. 4. ATPase 활성에 미치는 2가 양이온은 12.5㎛과 400㎛의 범위 내에서 이온 농도가 증가됨에 따라서 활성도는 점점 감소되었다. 5. ATPase활성율 50% 저해시키는데 2가 양이온의 농도는 Ki값과 같아서 ??, ??, ?? Pd ??에 대한 각각의 농도는 10㎛, 30㎛, 130㎛ 및 350㎛이었다. 특히 ??은 저 농도에서 (12.5-100㎛)는 활성제로, 고농도에서 (200-400㎛)는 저해제로 작용하는 것 같다. 6. 2가 양이온은 ATPase의 Km에는 변화를 주지 않으나 Vmax의 저하를 일으키는 것으로 보아 비경쟁 저해제임이 판정되었다. 7. 2가 양이온은 가역적 저해제임이 판명되었다. 8. Ca 吸收能에 대한 CuCl₂의 阻害는 50~500㎛의 CuCl₂농도에서 현저히 나타났으며 이 현상은 Ca 吸收能과 ATPase 活性과 밀접한 관계를 보여주는 것이다. General properties of the ATPase and the effects of the divalent cations (??) on the total ATPase activity of the fragmented sarcoplasmic reticulum (FSR) isolated from rabbit skeletal muscle were investigated in the present resent research. The inhibitory effect of divalent cations on the FSR ATPase activity and its mechanism were analyzed by various plots such as Dixon plot, Lineweaver-Burk plot, Hanes-woolf plot, Eadie-Scatchard plot and Arrhenius plot. The results obtained were as follows: 1. The FSR ATPase is activated by ?? and ??; the optimum concentration of is 0.1 mM in the presence of 4 mM MgCl₂. 2. The FSR ATPase could be distinguished as ??-ATPase and (??)-ATPase. The activity of (??)-ATPase predomiant over that of ??-ATPase. 3. The apparent energies of activation for total ATPase was approximately 19Kcal/mole. 4. The inhibitory effects of the divalent cations on the enzyme activity increased as the concentrations of the divalent cations increased in the concentrations between 12.5 and 400㎛. 5. Concentration of 50% inhibition for each divalent cation was almost identical with the inhibition constant (ki). The Ki's were 10,30,130, and 350㎛ for ??, ??, ??, and ??, respectively. ?? seemed to be anactivator at lower concentrations and an inhibior at higher concentratios. 6. The divalent cations did not change the Km but decreased the Vmax of the FRS ATPase, suggesting that they are noncompetitive inhibitors on the FSR ATPase. 7. The divalent cations were also found to be revesible inhibitors on the FSR ATPase. 8. The inhibitory effect of CuCl₂on a uptake increased as the concentration CuCl₂increased in the range between 50㎛.

관동맥질환에서 저밀도 지단백 입자 크기의 분포

임효영,김인숙,승기배,강동헌,전두수,장기육,박준철,이수영,홍순조,최규보 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.8

K이 흡착된 Si(111)7×7표면에 대한 RHEED 연구

박종윤,이순보,이경원,안기석,신익조,강건아 성균관대학교 1990 論文集 Vol.41 No.2

상온 및 200℃∼600℃의 Si(111) 7×7 표면에 칼륨(K)을 증착하였을 때의 표면격자구조 변화를 RHEED로 관측하였다. K 증착시 Si(111)7×7 기판의 온도가 상온인 경우, 어느 일정한 증착시간(포화덮임률에 도달했다고 추정되는 증착시간)이 지난 후에는 원래의 깨끗한 Si(111)7×7 패턴과 유사한 Si(111) 7×7-K 패턴이 관측되었고, 증착시간을 증가시켜도 RHEED 패턴은 변화하지 않았다. 이것을 annealing하면 350℃까지는 RHEED 패턴에 변화가 없다가 그 이상의 온도가 되면 서서히 원래의 7×7 패턴으로 되돌아가기 시작한다. Si(111) 7×7기판의 온도를 200℃∼600℃로 유지하면서 K을 일정시간(450℃에서 3×1이 형성되는 증착시간)이상 증착시킨 경우에 250℃까지는 상온의 경우와 비슷한 형태의 변형된 7×7 패턴이 관측되고, 300℃∼550℃일때는 3×1, 550℃ 이상에서는 1×1 구조가 관측되었다. 이때 300℃∼550℃에서 형성된 Si(111) 3×1-K 구조는 450℃에서 1분 정도 annealing 하면 항상 Si(111) 1×1-K 구조로 상전이가 일어남을 관측하였다. Potassium adsorbed surface structures of Si(111) 7×7 surface at room and high temperatures(200℃∼600℃) were investigated by RHEED. Potassium adsorption on the Si(111) 7×7 surface to saturated coverage at room temperature changed the RHEED pattern of Si(111) 7×7 to Si(111) 7×7-K. Subsequent heating of the Si(111) 7×7-K surface above 350℃ results in a Si(111) 7×7 with desorbing K. The RHEED pattern of the K-adsorbed on the Si(111) 7×7 surface up to the adsorption temperature of 250℃ was the modified 7×7 pattern, quite similar to that of the Si(111) 7×7-K, observed at room temperature. The 3×1 structure was observed in the temperature of adsorption between 300℃ and 550℃. Regardless of the adsorption temperature, a phase transition always took place from the Si(111) 3×1-K structure to Si(111) 1×1-K after annealing at 450℃ over 1 minute.

고내열 Polyester-imide Resin의 합성 및 열적 성질

박이순,하기룡,도정회 경북대학교 산업기술연구소 1993 産業技術硏究誌 Vol.21 No.-

Methylene dianiline(MDA)와 trimellitic anhydride (TMA)를 반응시켜 diacid diimide (DID)를 합성하고 이를 과량의 ethyleneglycol과 반응시켜 bishydroxyethylbisimide(BHEI) 중간체를 합성하였다. 다음 dimethylterephthalate와 ethyleneglycol를 에스테르 교환 반응시켜 bishydroxyethyl terephthalate(BHET)중간체를 합성하였고 이를 BHEI와 280℃, 감압(1~3 ㎜Hg)의 용융상태에서 축합 중합하여 polyester-imide를 얻었으며 GPC 및 ^1H-NMR로 분자량 및 공중합체 조성을 구하였다. 생성된 공중합체는 imide단위가 2-10 mole% 함유됨에 따라 PET단독중합체(T_g=78.9℃)보다 내열성(공중합체 T_g=81.9-91.6℃) 이 증가됨을 확인하였다. Methylene dianiline(MDA) was reacted with trimellitic anhydride(TMA) to give diacid diimide(DID) and then DID was reacted with excess of etyleneglycol to give bishydroxyethylbisimide(BHEI) intermediate. Dimethylterephthlate was reacted with ethyleneglycol by ester interchange reaction to give bishydroxyethylterephthalate(BHET) intermediate. BHET and BHEI was cocondensed in the melt state at 280℃ under reduced pressure (1~3 ㎜Hg) to give polyester-imide type copolymer. The molecular weight and composition of copolymer was determined by GPC and ^1H-NMR. The resulting copolymer showed increased thermal stability (T_g=81.9-91.6℃ with imide unite content of 2-10 ㏖%) compared to homopolymer (PET, T_g=78.9℃).

Cs이 흡착된 Si(111)7×7표면에 대한 RHEED연구

박종윤,이순보,이경원,안기석,강건아 성균관대학교 1991 論文集 Vol.42 No.1

Cesium-adsorbed surface structures on Si(111)7×7 were investigated at room and high temperatures(200∼700℃) by RHEED. The RHEED patterns of Si(111) 7×7 was changed to the modified 7×7 and the 1×1 patterns with increasing the deposition times of Cs at RT. It was observed that the structure of the Cs-adsorbed Si (111) 7×7 surface at saturation coverage is the 1×1 structure at RT. The ◁그림삽입▷(원문을 참조하세요) 1 and 1×1 structures appeared successively at the adsorption temperature of 300℃, 350∼400℃ and 450℃, respectively. After subsequent heating of 1×1 surface above 550℃ and of ◁그림삽입▷(원문을 참조하세요) surface above 600℃, each RHEED pattern gradually returned to the original Si(111)7×7 pattern. 상온 및 200∼700℃의 Si(111)7×7 표면에 Cs(Cesium)을 증착하였을 때 표면격자구조의 변화를 RHEED로 관측하였다. Cs 증착시 Si(111)7×7 기판의 온도가 상온인 경우, 포화 덮임률에 도달했다고 추정되는 일정 증착시간 전에는 원래의 깨끗한 Si(111)7×7 패턴과 거의 유사한 변형된(modified) Si(111)7×7-Cs 패턴이 관측되었다. 그후 포화 덮임률에서는 1×1패턴이 관측되고 증착량을 증가시켜도 패턴의 변화는 관측되지 않았다. 이 구조를 다시 annealing시키면 약 550℃부터 서서히 원래의 7×7구조로 되돌아가기 시작한다. Si(111)7×7기판의 온도를 220∼700℃로 유지하면서 Cs을 증착시킨 경우에 약 250℃까지는 상온에서와 비슷한 변형된(modified) 7×7이 관측되고 약 300℃에서는 ◁그림삽입▷(원문을 참조하세요) 350∼400℃ 정도에서는 ◁그림삽입▷(원문을 참조하세요)과 3×1이 겹쳐셔 관측되었다. 그리고 450℃ 이상에서는 1×1구조가 관측되었다. 이때 약 300℃에서 형성된 ◁그림삽입▷(원문을 참조하세요) 350℃에서 형성된 ◁그림삽입▷(원문을 참조하세요) 3+3×1 구조는 약 500℃ 정도까지 다시 annealing함에 따라 다리 1×1구조로 상전이가 일어난후, 약 600℃부터 원래의 7×7의 초격자점들이 나타나기 시작했다. 이들 결과로부터 Si(111)7×7 표면에 Cs을 증착하는 경우에는 일정한 포화 덮임률(saturation coverage)이 있는 것으로 추정되고, 이 덮임률에서 관측된 고온에서의 상전이는 증착량(증착시간)에는 무관하고 온도에만 의존함을 알 수 있었다. 또한 1×1 구조와 ◁그림삽입▷(원문을 참조하세요) 구조에 대하여 Cs의 탈착은 각각 약 550℃와 600℃에서 일어나기 시작하여 700℃에서 완전히 탈착됨을 알 수 있었다.

하경추부 손상환자에 대한 임상적 분석

박영록,홍순기,허철,김헌주,한용표 대한신경외과학회 1992 Journal of Korean neurosurgical society Vol.21 No.6

The authors analyzed 32 cases with lower cervical spine injuries according to their etiologies, the findings of cervical spine X-ray and computed tomography, initial neurological grading scales and time of operation. The results are summarized as follow : 1) Lower cervical spine injuries occurred in 32 of the 49 cervical spine injuried patients. 2) The important factors affecting on outcome were the initial neurological state(p=0.038) and the degree of spinal canal narrowing on cervical computed tomogram(p=0.046). 3) The degree of prevertebral soft tissue swelling and displacement of vertebral body on initial cervical spine X-ray did not significantly affect on outcome(p=0.052). 4) The most suitable operative time for lower cervical spine injuries was 10∼20 days after injuries and the mortality was 9.3%.

學生이 바라는 理想的 敎授像 : 文獻을 通해서 본

朴基順 東亞大學校 學生生活指導硏究所 1982 學生硏究 Vol.11 No.-

조직학습과 조직기억

박우순,정기찬 東亞大學校 2005 東亞論叢 Vol.42 No.-

Organizational learning and organizational memory are the primary components in knowledge management. In this paper, the major argument is to emphasize the role of organizational memory to make knowledge management successful. The contents of organizational memory are information, knowledge, and experiences held by organizational members obtained through organizational learning, and organizational memory need to be understood as an interrelated concepts for effective management of organizations.

APSM의 예측능 평가에 관한 연구

박기학,윤순창 한국환경과학회 2003 한국환경과학회지 Vol.12 No.3

The Pasquill-Gifford stability category is a very important scheme of the Gaussian type dispersion model defined the complex turbulence state of the atmosphere by A grade(very unstable) to F grade(very stable). But there has been made a point out that this stability category might decrease the predictability of the model because it was each covers a broad range of stability conditions, and that they were very site specific. The APSM (Air Pollution Simulation Model) was composed of the turbulent parameters, i.e. friction velocity( u_*), convective velocity scale( w_* ) and Monin-Obukhov length scale( L ) for the purpose of the performance increasing on the case of the unstable atmospheric conditions. And the PDF (Probability Density Function) model was used to express the vertical dispersion characteristics and the profile method was used to calculate the turbulent characteristics. And the performance assessment was validated between APSM and EPA regulatory models(TEM, ISCST), tracer experiment results. There were very good performance results simulated by APSM than that of TEM, ISCST in the short distance (<1415 m) from the source, but increase the simulation error(%) to stand off the source in others. And there were differences in comparison with the lateral dispersion coefficient( σ_y) which was represent the horizontal dispersion characteristics of a air pollutant in the atmosphere. So the different calculation method of σ_y, which was extrapolated from a different tracer experiment data might decrease the simulation performance capability. In conclusion, the air pollution simulation model showed a good capability of predict the air pollution which was composed of the turbulent parameters compared with the results of TEM and ISCST for the unstable atmospheric conditions.

조선업 근로자의 목부위 자각증상의 유병률과 관련 요인

박종,김기순,강명근,류소연,이철갑,강성득,김철호 朝鮮大學校 附設 醫學硏究所 2004 The Medical Journal of Chosun University Vol.29 No.1

Background and Objectives : This study examined prevalence and related factors of musculoskeletal symptoms on area of necks of shipbuilding workers in order to prevent musculoskeletal disorder and find out its managerial plans. Materials and Methods : this study targeted 1, 159 male shipbuilding workers who work for large suipbuilding companies, and interviewed them using organized questionnaire having short answer questions in February, 2003. Results: The prevalence of musculoskeletal symptoms (pains, anesthesia, and numbness) of the subjects was 16.0% on necks. As a result of multiple logistic regression analysis, the following results were obtained : sleeping hours, working departments, extra working hours, perceived amount of working, availability of dispensary were significantly related with symptoms on neck. When the variables that had significant relation with prevalence of neck area symptoms were analysed health behavior, it was found that the odds ratio of the group who had more than sufficient sleeping hours was 1.70 times higher than that of the group who had not. When they were examined in terms of occupational characteristics, the odds ratio of the subcontract company workers was 0.44 times lower than that of the parent company workers, and that of the group who had two hours of extra working hours was 1.78 times higher than that of the group who did not. The odds ratio of the group who thought the perceived working amount was slightly over the proper level was 1.49 time higher, and that of the group who thought it was excessively over the proper level was 3.21 times significantly higher. The odds ratio of the group who thought availability of dispensary was moderate was 1.84 times significantly higher than that of the group who thought it was easy. Conclusion : It was found that 16.0% of shipbuilding workers had musculoskeletal symptoms on neck, and their deom-sociological, occupational, and socio-psychological characteristics affected the symptoms. Therefore, to decrease musculoskeletal symptoms of shipbuilding workers, wording and socio-psychological condition should be improved by considering related factors of the workers.