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광기능성 폴리머의 소산장 결합을 이용한 측면연마 광섬유형 자외선 센서
김상우,서규원,윤종국,장수원,유윤식,이승하,강신원 한국센서학회 2002 센서학회지 Vol.11 No.6
측면연마된 광섬유와 광기능성 색소가 분산된 평면도파로 결합기를 이용한 새로운 형태의 자외선센서를 제작하여 그 특성을 측정하였다. 자외선 조사에 의해 유효굴절률이 변화하는 광변색성 색소가 분산된 폴리머를 평면 도파로 재료로 사용하였다. 박막 광도파로는 광변색성 색소인 스파이록사진을 사용하였다. 자외선 조사시간을 20초, 30초, 40초로 증가시켰을 경우 센서의 파장응답은 1.44nm/mW, 1.64nm/mW, 1.78nm/mW였고 1550nm 파장에서 센서의 90% 복귀시간은 100초로 나타났다. In this paper, we investigated a novel UV sensor using evanescent field coupling between the side polished fiber and photo-functional polymer waveguide. It was found that resonant wavelength shifts occur due to variation in the refractive index of polymer planar waveguide for its photo-functional properties on exposed UV. Spiroxazine (photochromic dye) was used as the planar waveguide. The resonant wavelength response were exhibited at 1.44 nm/mW, 1.64 nm/mW, and 1.78 nm/mW when UV irradiations were exposed for 20 seconds, 30 seconds, and 40 seconds, respectively. The recovery time of sensor was independent of UV exposure power and 90% recovery time was 100 seconds.
정인식,김호운,김진섭,이정희,이종현,신장규,박세일,권성원 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1
A planar chromel-alumel multijunction ac-dc converter was fabricated on a LPCVD Si_(3)N_(4)/SiO_(2)/Si_(3)N_(4)-diaphragm, prepared by silicon bulk micromachining, which thermally isolated a bifilar evanohm-heater and the hot junctions of a chromel-alumel thermopile from the silicon substrate. The voltage responsivity, the ac-dc transfer error, and the fluctuation of the output thermoelectric voltage of the converter were investigated. The respective voltage responsivities in air and in a vacuum of the converter were about 3.16 mV/mW and 9.41 mV/mW. The ac-dc voltage and the current transfer errors in air were about ±1.5 ppm and ±0.7 ppm in a frequency range from 10 Hz to 10 kHz, respectively. The fluctuation of the output thermoelectric voltage from 5 seconds to 120 seconds after preheating in air for 5 seconds was about 0.06 % for a heater input of dc 1 V.
섬유보강 그라우트재를 이용한 다철근 네일의 보강효과에 관한 연구
신상훈(Sang-Hoon Shin),박태규(Tae-Gyoo Park),강세구(Se-Gu Kang),이상덕(Sang-Duk Lee) 한국지반신소재학회 2013 한국토목섬유학회 학술발표회 Vol.2013 No.11
The soil nailing method is passive reinforcement which the soil resistance is exerted according to the displacement caused. Thus, If the displacement occurs over a certain value at failure surface by bending and shear stress, the grout will be brittle failure. In this study, reinforced fiber in grout was adopted to prevent brittle failure, and large scale shear test and field pullout test was conducted for performance evaluation. As a result, multi-rebar nail of using fiber-reinforced cement paste is improve in bending, shear resistance compared with normal soil nailing. And also, it was found to increase the pullout resistance.
Shin, Gi Won,Hwang, Hee Sung,Nam, Hong Gil,Oh, Mi-Hwa,Jung, Gyoo Yeol Wiley Subscription Services, Inc., A Wiley Company 2010 Biotechnology and Bioengineering Vol.106 No.1
<P>Quantification of RNA provides information crucial for various biological studies, including analysis of mRNA expression and that of microRNAs. Reverse transcription (RT) coupled with real-time polymerase chain reaction (PCR) is known to be the most accurate method for quantifying nucleic acids, and thus represents the state-of-the-art for RNA quantification. However, the use of real-time PCR for RNA quantification is limited to a single target per analytical run because of reductions in quantification power and limitations of fluorescence dyes associated with multiplex applications. Here, we report a novel multiplex RNA quantification method that uses capillary electrophoresis single-strand conformation polymorphism (CE-SSCP) coupled with modified RT and asymmetric PCR. The reverse transcripts of seven in vitro transcribed RNAs were modified with common sequence tags and amplified by asymmetric PCR using primers specific to the common tags. The resulting amplicons were separated and quantified by CE-SSCP. A series of experiments using different amounts of RNA demonstrated that the assay had a limit of detection of 2 amol and a dynamic range of ∼10<SUP>5</SUP>. These results clearly indicate the potential of this method to provide robust and precise multiplex RNA quantification. Biotechnol. Bioeng. 2010; 106: 167–172. © 2009 Wiley Periodicals, Inc.</P>
Single-Electron-Based Flexible Multivalued Exclusive-<small>or</small> Logic Gate
Sang-Jin Kim,Chang-Keun Lee,Rae-Sik Chung,Eun-Sil Park,Seung-Jun Shin,Jung-Bum Choi,Yun-Seop Yu,Nam-Soo Kim,Hyung Gyoo Lee,Keun-Hyung Park IEEE 2009 IEEE transactions on electron devices Vol.56 No.5
<P>By using two symmetrical sidewall gates, we implemented a Si-based single-electron exclusive- OR (XOR) gate and reported on the first flexible multivalued (MV) functionality. A grayscale contour plot of the output voltages displays alternating high/low values as a function of two single-electron transistor (SET) input voltages. Their voltage transfer characteristics display typical XOR or XNOR gate function depending on input voltages for binary, MV, and binary-MV mixed-modes. This flexible two-input XOR gate, combined with the previously reported NAND/NOR gates, provide three basic arithmetic blocks for the SET-based MV logic gate family.</P>
Shin, Gi Won,Cho, Yang Sook,Hwang, Hee Sung,Oh, Mi-Hwa,Nam, Hong Gil,Park, Jin Hyun,Jung, Gyoo Yeol WILEY-VCH Verlag 2009 Electrophoresis Vol.30 No.15
<P>Rapid diagnosis of bacterial infection is important for patient management and appropriate therapy during the early phase of bacteria-induced disease. Among the existing techniques for identifying microbial, CE-SSCP combined with 16S ribosomal RNA gene-specific PCR has the benefits of excellent sensitivity, resolution, and reproducibility. However, even though CE-SSCP can separate PCR products with high-resolution, multiplex detection and quantification are complicated by primer-dimer formation and non-specific amplification. Here, we describe a novel technique for multiplex detection and quantification of pathogens by template-tagging followed by multiplex asymmetric PCR and subsequent CE-SSCP. More specifically, we reverse transcribed 16S ribosomal RNAs from seven septicemia-inducing pathogens, tagged the templates with common end sequences, and amplified them using common primers. The resulting amplicons could be successfully separated by CE-SSCP and quantified by comparison to an internal standard. This method yielded results that illustrate the potential of this system for diagnosing infectious disease.</P>
Recent developments in CE-based detection methods for food-borne pathogens
Shin, Gi Won,Hwang, Hee Sung,Chung, Boram,Jung, Gyoo Yeol WILEY-VCH Verlag 2010 Electrophoresis Vol.31 No.13
<P>Rapid and sensitive detection of food-borne pathogens is critical for food safety from the viewpoint of both the public health professionals and the food industry. Conventional method is, however, known to be labor-intensive, time-consuming, and expensive due to the separate cultivation and biochemical assay. Many relevant technologies, such as flow cytometry, MALDI-MS, ESI-MS, DNA microarray, and CE, have been intensively developed to date. Among them, CE is considered to be the most efficient and reproducible because of low sample loss and simple automation. CE-based pathogen detection methods can be classified into three categories based on the separation targets: cell separation, nucleic-acid-based identification, and protein separation coupled with characterization. In this review, recent developments in each sphere of CE-based technology are discussed. Additionally, the critical features of each approach and necessary future technical improvements are also reviewed.</P>
( Shin Ju Oh ),( Chun Gyoo Ihm ),( Tae Won Lee ),( Jin Sug Kim ),( Da Rae Kim ),( Eun Ji Park ),( Su Woong Jung ),( Ji-hoon Lee ),( Sung Hyuk Heo ),( Kyung Hwan Jeong ) 대한신장학회 2017 Kidney Research and Clinical Practice Vol.36 No.1
The syndrome of inappropriate antidiuretic hormone secretion (SIADH) is a potential cause of hyponatremia of the central nervous system (CNS). Although SIADH has been reported to be associated with many other central nervous disorders, its association with neuromyelitis optica (NMO) or NMO spectrum disorders are rare. NMO is a demyelinating disorder characterized by optic neuritis and transverse myelitis. Aquaporin-4 (AQP4), which is the target antigen for a NMO autoantibody, is the predominant CNS water channel. However, some NMO patients show seronegative AQP4 antibody results. The spectrum of NMO has been changed, and new findings about the disease have been reported. Here, we report a case of seronegative NMO spectrum disorder associated with SIADH.
The mechanism of apoptosis induced by eugenol in human osteosarcoma cells
Sang-Hun Shin,Jae-Hyun Park,Gyoo-Cheon Kim,Bong-Soo Park,Young-Gi Gil,Chul-Hoon Kim 대한구강악안면외과학회 2007 대한구강악안면외과학회지 Vol.33 No.1
Eugenol is commonly used in dentistry for the sedation of toothache, pulpitis, and dental hyperalgesia. This study was performed to investigate the apoptotic effect of eugenol to human osteosarcoma (HOS) cells and the potential use of this compound in osteosarcoma cells. Eugenol showed the apoptotic effect in HOS cells in dose- and time-dependent manner. Fragmentation and condensation of DNA were showed by TUNEL assay, Hemacolor stain and Hoechst stain. In the DNA electrophoresis analysis, cells showed DNA degradation characteristic of apoptosis with a ladder pattern of DNA fragments. Apoptosis-related factors were analyzed by western blotting. Cells treated with eugenol showed caspase-3, PARP, lamin A and DFF-45 cleavage. Eugenol treatment induced caspase-3 cleavage and activation. Cleavages of PARP, DFF-45 and lamin A were accompanied with activation of caspase triggered by eugenol in HOS cells. Though this study needs more investigations, these results suggest that eugenol induce apoptosis via caspase dependent pathway in HOS cells and eugenol may constitute a potential antitumor compound against osteosarcoma cells.
A novel pathogen detection system based on high-resolution CE-SSCP using a triblock copolymer matrix
Shin, Gi Won,Hwang, Hee Sung,Seo, Sang Woo,Oh, Mi-Hwa,Ryu, Chang Y.,Salvo, Charles J.,Feldman, Shaina,Doh, Junsang,Jung, Gyoo Yeol WILEY-VCH Verlag 2010 Journal of Separation Science Vol.33 No.11
<P>Although CE-SSCP analysis combined with 16S ribosomal RNA gene-specific PCR has enormous potential as a simple and versatile pathogen detection technique, low resolution of CE-SSCP causes the limited application. Among the experimental conditions affecting the resolution, the polymer matrix is considered to be most critical to improve the resolution of CE-SSCP analysis. However, due to the peak broadening caused by the interaction between hydrophobic moiety of polymer matrices and DNA, conventional polymer matrices are not ideal for CE-SSCP analysis. A poly(ethyleneoxide)-poly(propyleneoxide)-poly(ethyleneoxide) (PEO-PPO-PEO) triblock copolymer, with dynamic coating ability and a propensity to form micelles to minimize exposure of hydrophobic PPO block to DNA, can be an alternative matrix. In this study, we examined the resolution of CE-SSCP analysis using the PEO-PPO-PEO triblock copolymer as the polymer matrix and four same-sized DNA fragments of similar sequence content. Among 48 commercially available PEO-PPO-PEO triblock copolymers, three were selected due to their transparency in the operable range of viscosity and PEO<SUB>137</SUB>PPO<SUB>43</SUB>PEO<SUB>137</SUB> exhibited the most effective separation. Significant improvement in resolution allowed discrimination of the similar sequences, thus greatly facilitated CE-SSCP analysis compared to the conventional polymer matrix. The results indicate that PEO-PPO-PEO triblock copolymer may serve as an ideal matrix for high-resolution CE-SSCP analysis.</P>