Analysis the role of arabidopsis CKRC6/ASA1 in auxin and cytokinin biosynthesis

Dong-Wei Di,Lei Wu,Pan Luo,Li Zhang,Tian-Zi Zhang,Xue Sun,Shao-Dong Wei,Chen-Wei An,Guang-Qin Guo 한국식물학회 2016 Journal of Plant Biology Vol.59 No.2

The crosstalk between auxin and cytokinin (CK) is important for plant growth and development, although the underlying molecular mechanisms remain unclear. Here, we describe the isolation and characterization of a mutant of Arabidopsis Cytokinin-induced Root Curling 6 (CKRC6), an allele of ANTHRANILATE SYNTHASE ALPHA SUBUNIT 1 (ASA1) that encodes the á-subunit of AS in tryptophan (Trp) biosynthesis. The ckrc6 mutant exhibits root gravitropic defects and insensitivity to both CK and the ethylene precursor 1-aminocyclopropane-1-carboxylicacid (ACC) in primary root growth. These defects can be rescued by exogenous indole-3-acetic acid (IAA) or tryptophan (Trp) supplementation. Furthermore, our results suggest that the ckrc6 mutant has decreased IAA content, differential expression patterns of auxin biosynthesis genes and CK biosynthesis isopentenyl transferase (IPT) genes in comparison to wild type. Collectively, our study shows that auxin controls CK biosynthesis based on that CK sensitivity is altered in most auxin-resistant mutants and that CKs promote auxin biosynthesis but inhibit auxin transport and response. Our results also suggest that CKRC6/ASA1 may be located at an intersection of auxin, CK and ethylene metabolism and/or signaling.

Stable pH sensitivity of LaAlO3/SrTiO3 interfacial electronic gas

Dong Yan,Zhang Lunyong,Li Chen,Liu Yanli,Shao Pengfei,Lei Jianming,Wang Rui,Wu Di,Chen Dunjun,Zhang Rong,Zheng Youdou 한국물리학회 2022 Current Applied Physics Vol.34 No.-

Probing pH value is essential for many applications. The request of developing stable and highly sensitive pH sensor with small size are increased recently, this is a challenge to the traditional glass pH sensors. This work investigated the pH sensing performance of the novel two-dimensional electronic gas (2DEG) at the LaAlO3/ SrTiO3 heterostructure interface discovered recently. The experimental results demonstrated that the devices host excellent sensing ability to the pH value of aqueous solutions. Quite stable output current is realized for a given pH value, and the output current is linearly dependent on pH value as required by sensor applications. This work would prompt the application research of LaAlO3/SrTiO3 heterostructure and design of new generation of advanced pH sensors.

Sesquiterpenoids and 2‐(2‐Phenylethyl)‐4<i>H</i>‐chromen‐4‐one (=2‐(2‐Phenylethyl)‐4<i>H</i>‐1‐benzopyran‐4‐one) Derivatives from <i>Aquilaria malaccensis</i> Agar

Wu, Bo,Lee, Jin Gyun,Lim, Chang Ju,Jia, Shao Dong,Kwon, Sung Won,Hwang, Gwi Seo,Park, Jeong Hill WILEY‐VCH Verlag 2012 Helvetica chimica acta Vol.95 No.4

<P><B>Abstract</B></P><P>The four new sesquiterpenoids <B>1</B>–<B>4</B>, and the new 2‐(2‐phenylethyl)‐4<I>H</I>‐chromen‐4‐one (=2‐(2‐phenylethyl)‐4<I>H</I>‐1‐benzopyran‐4‐one) derivative <B>5</B>, together with the two known sesquiterpenoids <B>6</B> and <B>7</B>, the five known chromenones <B>8</B>–<B>12</B>, and 1‐hydroxy‐1,5‐diphenylpentan‐3‐one (<B>13</B>), were isolated from a 70% MeOH extract of <I>Aquilaria malaccensis</I> agarwood chips. Their structures were elucidated on the basis of comprehensive spectral analyses and comparison with literature data.</P>

중국 외자정책 전환의 필요성 연구: 규모조절, 외자선택, 내국민대우

무소준 ( Shao Jun Wu ),동초 ( Chao Dong ) 한중사회과학학회 2005 한중사회과학연구 Vol.5 No.-

지난 20여 년간 중국은 외자유치방면에서 커다란 성과를 거두었으며, 외자는 중국 경제성장에 큰 기여를 하였다. 존량(stock) 측면에서 2004년 현재 중국의 외자이용액은 5,621억 달러에 달하였다. 유량(flow) 측면에서 보면 중국의 외자이용액은 전체적으로 매년 증가하여, 2004년 중국이 각종 형식으로 이용한 외자규모는 640.7억 달러이다. 그 중 외국인 직접투자규모는 606.3억 달러로 미국 다음인 세계 2위 수준이다. 중국이 외국인직접투자를 이용하는 과정에서 외자정책은 중국정부가 외자유량을 통제하고 외자흐름을 이끄는 중요한 수단이다.

Numerical and experimental study of cone-headed projectile entering water vertically based on MMALE method

Cao Miaomiao,Shao Zhiyu,Wu Siyu,Dong Chaochao,Yang Xiaotian 대한조선학회 2021 International Journal of Naval Architecture and Oc Vol.13 No.1

The water entry behaviors of projectiles with different cone-headed angles were studied numerically, experimentally and theoretically, mainly focusing on the hydrodynamic impact in the initial stage. Based on MMALE algorithm, it was proposed a formula of impact deceleration, which relied on the initial entry velocity and cone-headed angle. Meanwhile, in order to verify the validity of the simulation model, experiments using accelerometer and high-speed camera were carried out, and their results were in a good agreement with simulation results. Also, theoretical calculation results of cavity diameter were compared with experiments and simulation results. It was observed that the simulation method had a good reliability, which would make forecast on impact deceleration in an engineering project.

Intranasally Administered Adjunctive Dexmedetomidine Reduces Perioperative Anesthetic Requirements in General Anesthesia

Zheng Chen,Xiang Wu,Li-Hua Hang,Hong Wang,Dong-Hua Shao,Yi-Guo Xu,Wei Cui 연세대학교의과대학 2016 Yonsei medical journal Vol.57 No.4

Purpose: Intranasal dexmedetomidine is an effective sedative for premedication and is regularly used to reduce preoperative tension and anxiety in children. This study aimed to assess the effect of intranasally adjunctive dexmedetomidine on perioperativesedative and analgesic requirements in adults. Materials and Methods: Patients were randomly divided into four groups to receive preoperative administration of saline, intranasaldexmedetomidine 1 μg/kg and 2 μg/kg, and intravenous dexmedetomidine 1 μg/kg, respectively. Propofol and remifentanil were target-controlled infused to maintain intraoperative bispectral index at 45–55 and blood pressure at baseline value±20%. Sufentanil was administered to maintain postoperative visual analogue scale ≤3. Perioperative anesthetics requirements were compared using nonparametric tests. Results: Intranasal dexmedetomidine significantly attenuated propofol requirements for anesthesia induction and maintenance in a dose-dependent manner. Patients given intranasal dexmedetomidine 2 μg/kg required less remifentanil for anesthesia maintenance. The first postoperative request for sufentanil analgesia was delayed in patients given intranasal dexmedetomidine 2 μg/kg. The anesthetics-sparing effect of intranasal dexmedetomidine was significantly weaker than intravenous dexmedetomidine at the same dose of 1 μg/kg. The incidences of adverse events, including hemodynamic instability and delayed recovery, were comparablewith and without intranasal dexmedetomidine. Conclusion: Intranasal administration of dexmedetomidine can reduce perioperative anesthetic requirements, and a dose of dexmedetomidine 2 μg/kg produces a better effect in adults. The anesthetics-sparing effect of intranasal dexmedetomidine 1 μg/kg is less than that with the same intravenous dose of dexmedetomidine.

Non-Doped Organic Light-Emitting Diodes with Saturated Red Emission

Fei Xiao,Bing-xian Shao,Huan-rong Wu,Hui-ying Fu,Xiao-yuan Hou,Xin-dong Gao,Yi-qiang Zhan 한국물리학회 2007 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.50 No.2

Non-doped organic light-emitting diodes with saturated red emission were fabricated using 4-(2-(3,3-dicyanomethylene-5,5-dimethyl-1-cyclohexylidene)vinyl)phenyldi(1-naphthyl)amine (DNP-2CN) or 4-(2-(3,3-dicyanomethylene-5,5-dimethyl-1-cyclohexylidene)vinyl)phenyl(1-naphthyl)phe- nylamine (DPN-2CN) as the emitting layer. Different electron-transporting materials, tris(8-hydroxylquinoline) aluminum (Alq$_3$), 2,2',2''-(1,3,5-phenylene)tris[1-phenyl-1$H$-benzimidazole] (TPBI) and 2-(4-biphenyl)-5-(4-tert-butylphenyl)-1,3,4-oxadiazole (PBD), were introduced into the devices for examining their energy level compatibility of DNP-2CN or DPN-2CN. The device with a structure of ITO/ NPB/ DNP-2CN/ BCP/ Alq$_3$/ LiF/ Al showed red emission with $\lambda_{max}$ at 670 nm (CIE coordinates: $x$ = 0.66, $y$ = 0.33) and a high luminance of 438 cd m$^{-2}$ at a driving voltage of 12 V. The device with a structure of ITO/ NPB/ DPN-2CN/ BCP/ Alq$_3$/ LiF/ Al showed a high brightness of 225 cd m$^{-2}$ at a driving voltage of 12 V with $\lambda_{max}$ at 674 nm (CIE coordinates: $x$ = 0.65, $y$ = 0.33).

A Prediction Model of the Sum of Container Based on Combined BP Neural Network and SVM

Ding, Min-jie,Zhang, Shao-zhong,Zhong, Hai-dong,Wu, Yao-hui,Zhang, Liang-bin Korea Information Processing Society 2019 Journal of information processing systems Vol.15 No.2

The prediction of the sum of container is very important in the field of container transport. Many influencing factors can affect the prediction results. These factors are usually composed of many variables, whose composition is often very complex. In this paper, we use gray relational analysis to set up a proper forecast index system for the prediction of the sum of containers in foreign trade. To address the issue of the low accuracy of the traditional prediction models and the problem of the difficulty of fully considering all the factors and other issues, this paper puts forward a prediction model which is combined with a back-propagation (BP) neural networks and the support vector machine (SVM). First, it gives the prediction with the data normalized by the BP neural network and generates a preliminary forecast data. Second, it employs SVM for the residual correction calculation for the results based on the preliminary data. The results of practical examples show that the overall relative error of the combined prediction model is no more than 1.5%, which is less than the relative error of the single prediction models. It is hoped that the research can provide a useful reference for the prediction of the sum of container and related studies.

Effect of Trichostatin A on CNE2 Nasopharyngeal Carcinoma Cells - Genome-wide DNA Methylation Alteration

Yang, Xiao-Li,Zhang, Cheng-Dong,Wu, Hua-Yu,Wu, Yong-Hu,Zhang, Yue-Ning,Qin, Meng-Bin,Wu, Hua,Liu, Xiao-Chun,Lina, Xing,Lu, Shao-Ming Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.11

Trichostatin A (TSA) is a histone deacetylase (HDAC) inhibitor. We here investigated its effects on proliferation and apoptosis of the CNE2 carcinoma cell line, and attempted to establish genome-wide DNA methylation alteration due to differentially histone acetylation status. After cells were treated by TSA, the inhibitory rate of cell proliferation was examined with a CCK8 kit, and cell apoptosis was determined by flow cytometry. Compared to control, TSA inhibited CNE2 cell growth and induced apoptosis. Furthermore, TSA was found to induce genome-wide methylation alteration as assessed by genome-wide methylation array. Overall DNA methylation level of cells treated with TSA was higher than in controls. Function and pathway analysis revealed that many genes with methylation alteration were involved in key biological roles, such as apoptosis and cell proliferation. Three genes (DAP3, HSPB1 and CLDN) were independently confirmed by quantitative real-time PCR. Finally, we conclude that TSA inhibits CNE2 cell growth and induces apoptosis in vitro involving genome-wide DNA methylation alteration, so that it has promising application prospects in treatment of NPC in vivo. Although many unreported hypermethylated/hypomethylated genes should be further analyzed and validated, the pointers to new biomarkers and therapeutic strategies in the treatment of NPC should be stressed.

Berberine suppresses in vitro migration of human aortic smooth muscle cells through the inhibitions of MMP-2/9, u-PA, AP-1, and NF-κB

( Su Jian Liu ),( Cai Xia Yin ),( Ming Chao Ding ),( Shao You Xia ),( Qin Min Shen ),( Ji Dong Wu ) 생화학분자생물학회(구 한국생화학분자생물학회) 2014 BMB Reports Vol.47 No.7

Berberine, a type of isoquinoline alkaloid isolated from Chinese medicinal herbs, has been reported to have various pharmacological activities. Studies have demonstrated that berberine has beneficial effects on vascular remodeling and alleviates restenosis after vascular injury. However, its mechanism of action on vascular smooth muscle cell migration is not fully understood. We therefore investigated the effect of berberine on human aortic smooth muscle cell (HASMC) migration. Boyden chamber assay was performed to show that berberine inhibited HASMC migration dose- dependently. Real-time PCR and Western blotting analyses showed that levels of matrix metalloproteinase (MMP)-2, MMP-9, and urokinase-type plasminogen activator (u-PA) were reduced by berberine at both the mRNA and protein levels. Western blotting assay further confirmed that activities of c-Fos, c-Jun, and NF-κB were significantly attenuated. These results suggest that berberine effectively inhibited HASMC migration, possibly by down-regulating MMP-2, MMP-9, and u-PA; and interrupting AP-1 and NF-κB mediated signaling pathways. [BMB Reports 2014; 47(7): 388-392]