Analysis the role of arabidopsis CKRC6/ASA1 in auxin and cytokinin biosynthesis

Dong-Wei Di,Lei Wu,Pan Luo,Li Zhang,Tian-Zi Zhang,Xue Sun,Shao-Dong Wei,Chen-Wei An,Guang-Qin Guo 한국식물학회 2016 Journal of Plant Biology Vol.59 No.2

The crosstalk between auxin and cytokinin (CK) is important for plant growth and development, although the underlying molecular mechanisms remain unclear. Here, we describe the isolation and characterization of a mutant of Arabidopsis Cytokinin-induced Root Curling 6 (CKRC6), an allele of ANTHRANILATE SYNTHASE ALPHA SUBUNIT 1 (ASA1) that encodes the á-subunit of AS in tryptophan (Trp) biosynthesis. The ckrc6 mutant exhibits root gravitropic defects and insensitivity to both CK and the ethylene precursor 1-aminocyclopropane-1-carboxylicacid (ACC) in primary root growth. These defects can be rescued by exogenous indole-3-acetic acid (IAA) or tryptophan (Trp) supplementation. Furthermore, our results suggest that the ckrc6 mutant has decreased IAA content, differential expression patterns of auxin biosynthesis genes and CK biosynthesis isopentenyl transferase (IPT) genes in comparison to wild type. Collectively, our study shows that auxin controls CK biosynthesis based on that CK sensitivity is altered in most auxin-resistant mutants and that CKs promote auxin biosynthesis but inhibit auxin transport and response. Our results also suggest that CKRC6/ASA1 may be located at an intersection of auxin, CK and ethylene metabolism and/or signaling.

What is the relationship between executive compensation incentive and earnings management in Listed Companies in China?

( Hu Di ),( Huang Wei Dong ),( Jin Shan Yue ) 한국외국어대학교 중국연구소 2021 中國硏究 Vol.89 No.-

This study investigates the impact of executive compensation incentive on earnings management in Chinese listed companies. From the perspectives of the principal-agent, asymmetric information, and incentive theories, this study examines Chinese A-share, main board-listed companies from 2015 to 2019 for the impact of executive compensation incentive on corporate earnings management, using a multiple linear regression method. The results show that executive monetary compensation has a negative effect on the level of accrual-based earnings management and real earnings management, indicating that executive monetary compensation has an incentive effect and reduces earnings management behavior. However, the executive shareholding ratio has a positive impact on accrual-based earnings management but no significant impact on real earnings management, indicating that equity incentives only affect accrual-based earnings management. The study’s findings enrich the related theories of executive compensation and earnings management and provide useful insights for investors and policymakers of listed companies.

Seismic Behavior of Low-rise Concrete Shear Wall with Single Layer of Web Reinforcement and Inclined Rebars: Restoring Force Model

Jian-Wei Zhang,Wen-Bin Zheng,Wan-Lin Cao,Hong-Ying Dong,Wan-Di Li 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.3

In order to study the restoring force model of low-rise concrete shear wall with single layer of web reinforcement and inclined rebars, a series of specimens were investigated by quasi-static tests. Based on the fitting of experimental data and theoretical analysis, the restoring force model considering four characteristic points (crack point, yield point, peak point and failure point) and degradation of unloading stiffness was established. The hysteretic rule of restoring force model was determined by analyzing characteristic of hysteresis curve for cyclic loading tests. The results show that skeleton curves and hysteresis curves calculated by the restoring force model are in good agreement with the test curves, which can provide reference for the elastic-plastic dynamic analysis of low-rise concrete shear walls with single layer of web reinforcements and inclined rebars. Shaking table tests of two low-rise concrete shear walls were also conducted to investigate dynamic performance and the seismic damage mechanism of low-rise concrete shear wall with single layer of web reinforcement and inclined rebars. Research on dynamic nonlinear simplified model of SAP2000 shows thatthe proposed restoring force model can be used to analyze the elastic and elastic-plastic dynamic response for the low-rise concrete shear wall with single layer of web reinforcement and inclined rebars.

NF-jB activation was involved in reactive oxygen speciesmediated apoptosis and autophagy in 1-oxoeudesm-11(13)-eno- 12,8a-lactone-treated human lung cancer cells

Shanshan Liu,Di Wu,Lin Li,Xiao Sun,Wei-Dong Xie,Xia Li 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.8

1-oxoeudesm-11(13)-eno-12,8a-lactone (OEL),a novel eudesmane-type sesquiterpene compound, has beenshown to inhibit the growth of some cancer cell lines andinduce significant apoptosis. Here, we investigated the anticanceractivities of OEL in human lung cancer cells. Ourstudies demonstrated that OEL induced both apoptosis andautophagy in A549 and H460 cells. OEL-induced autophagywas assessed by appearance of autophagic vacuoles,formation of acidic vesicular organelles, conversion ofLC3-I to LC3-II, recruitment of LC3-II to the autophagosomes,and activation of autophagy genes. Furthermore,administration of autophagic inhibitor 3-methyladenineaugments OEL-induced apoptotic cell death. The inductionof autophagy and apoptosis by OEL links to NF-jB activationand the generation of reactive oxygen species (ROS). Interruption of NF-jB activation by specific inhibitor promotesapoptosis, but decreases autophagy. ROS antioxidants(N-acetylcysteine) attenuated both OEL-inducedautophagy and apoptosis. Further experiments confirmedthat OEL-induced activation of ROS was increased by NFjBinhibitor whereas NF-jB activation was not affected by ROS inhibition. These findings suggest that OEL-elicitedautophagic response plays a protective role that impedescell death, and inhibition of autophagy could be anadjunctive strategy for enhancing the chemotherapeuticeffect of OEL as an antitumor agent.

Microwave-assisted Approach for the Rapid Enzymatic Digestion of Rapeseed Meal

Ju-Fang Li,Fang Wei,Lu-Lu Guo,Gang-You Yuan,Feng-Hong Huang,Mu-Lan Jiang,Yuan-Di Zhao,Xu-Yan Dong,Guang-Ming Li,Hong Chen 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.2

This study demonstrates the use of a new microwave-assisted approach for accelerating the enzymatic digestion of rapeseed meal. The effects of different microwave parameters, such as the time, temperature, and power level, on the degree of hydrolysis (DH) were investigated by using response surface methodology (RSM). The maximum predicted DH value (10.2%) was in good agreement with the value obtained experimentally using an alkaline protease, which was 12.57% under optimal conditions. In only 7 min, the microwave-assisted method achieved a DH value similar to that obtained by the conventional enzymatic digestion method (4 hr). Therefore,this new technique for rapid enzymatic digestion will improve the application of rapeseed meal in the preparation of protein hydrolysates for use in food and feed.

Genetic Association between the XPG Asp1104His Polymorphism and Head and Neck Cancer Susceptibility: Evidence Based on a Meta-Analysis

Jiang, Hua-Yong,Zeng, Yong,Xu, Wei-Dong,Liu, Chuan,Wang, Ya-Jie,Wang, Ya-Di Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.9

Background: Previous studies evaluating the association between the xeroderma pigmentosum group G (XPG) Asp1104His polymorphism and head and neck cancer susceptibility have proven controversial. This meta-analysis of the literature was performed to obtain a more precise estimation of the relationship. Materials and Methods: We systematically searched PubMed, Embase and Web of Science with a time limit of Dec 18, 2014. Odds ratios (ORs) with 95% confidence intervals (CIs) were used to assess the strength of any association. Results: We performed a meta-analysis of eight published case-control studies, including 3,621 cases and 5,475 controls. Overall, no significant association was found between the XPG Asp1104His polymorphism and head and neck cancer susceptibility under all genetic models. In the subgroup analysis by ethnicity, the XPG Asp1104His polymorphism had statistically significant association with elevated head and neck cancer risk under CC vs GG (OR=1.24, 95% CI=1.00~1.54) and the recessive model (OR=1.22, 95%CI=1.01~1.46) in Asian populations. A similar result was found under CC vs GG (OR =1.22, 95%CI=1.01~1.47) in the population based subgroup by source of control. When performed by tumor site, the XPG Asp1104His polymorphism had statistically significant association with elevated laryngeal cancer under all genetic models (CC vs GG: OR=1.59, 95% CI=1.16~2.19; GC vs GG: OR=1.38, 95%CI=1.10~1.72; dominant model: OR=1.42, 95% CI=1.15~1.74; recessive model: OR=1.36, 95% CI=1.02~1.81). Conclusions: This meta-analysis suggested that the XPG Asp1104His polymorphism is a risk factor for head and neck cancer susceptibility, especially for laryngeal cancer and in Asian populations.

Fluorescent Nitrogen-Doped Carbon Dots via Single-Step Synthesis Applied as Fluorescent Probe for the Detection of Fe3+ Ions and Anti-Counterfeiting Inks

Chonghui Fan,Kelong Ao,Pengfei Lv,Jiancheng Dong,Di Wang,Yibing Cai,Qufu Wei,Yang Xu 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.08

Fluorescent nitrogen-doped carbon dots (N-CDs) with excellent stability were prepared via single-step hydrothermal carbonization of citric acid (CA) and ethylenediamine (EDA). The as-prepared N-CDs emit blue fluorescence under the excitation of 365 nm and have a size distribution of 2.80 ffi 0.47 nm with benign size effect. The structure and morphology were further characterized by X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR) spectroscopy. It was found that the surface of the N-CDs was successfully functionalized, which presented water solubility and chelation with Fe3+. XRD results display a diffraction peak at 23.9 ℃, which corresponds to the (002) interlayer spacing of a graphitic structure revealing an amorphous carbon phase. Furthermore, due to good sensitivity, N-CDs were used as probes for Fe3+ detection. The low limit of detection of 0.6 μM as a fluorescence probe was successfully obtained based on the linear relationship between (F0 - F) / F0 and concentration of Fe3+ ions. Besides the satisfactory fluorescence, PVA/N-CDs membranes and fluorescent inks demonstrate potential for anti-counterfeiting applications due to its characteristic flexibility, transparency, removability and invisibility under ambient lighting.

Association between the CYP1A2 rs762551 Polymorphism and Bladder Cancer Susceptibility: a Meta-Analysis Based on Case-Control Studies

Zeng, Yong,Jiang, Hua-Yong,Wei, Li,Xu, Wei-Dong,Wang, Ya-Jie,Wang, Ya-Di,Liu, Chuan Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.16

Background: Previous studies evaluated associations between the CYP1A2 rs762551 polymorphism and bladder cancer risk. However, the results were inconsistent. We therefore performed a meta-analysis of the published case-control studies to assess in detail the association between CYP1A2 rs762551 polymorphism and bladder cancer risk. Materials and Methods: PubMed, Embase and Web of Science were searched to identify relevant studies and the pooled odds ratio (OR) and 95 % confidence interval (95%CI) were calculated. Results: A total of seven articles including 3,013 cases and 2,771 controls were finally included. Overall, a significant association was found between the CYP1A2 rs762551 polymorphism and bladder cancer susceptibility for CC vs AA (OR=0.82, 95% CI=0.69~0.99), but no significant associations were found for the other three models (AC vs AA: OR=0.91, 95% CI=0.81~1.02; the dominant model: OR=0.90, 95% CI=0.80~1.00; the recessive model: OR=0.84, 95% CI =0.72~1.00). In the subgroup analysis by ethnicity, we detected significant associations between the CYP1A2 rs762551 polymorphism and bladder cancer susceptibility for GA vs GG (OR = 0.78, 95% CI =0.64~0.96) and for the recessive model (OR=0.80, 95% CI=0.66~0.96) in Caucasians, but not for Asians. Conclusions: The results from the meta-analysis suggested that the CYP1A2 rs762551 polymorphism is a protective factor for bladder cancer, especially in Caucasians.