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Nam Sook Park,Han Seok Kang,Seon Ku Kim,Yong Gyun Kim,Byung Uuk Cho,Teak Soon Shin,Keun Ki Kim,Hyun Chul Park,Hong Joo Son,Hong Gu Lee,Sang Mong Lee 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.05
Presently, We have constructed an olig-d(T) primed directional cDNA library from the silkworm Dongchunghacho, an entomopathogenic fungus, of which species is belonging to Paecilomyces tenuipes Jocheon-1. To isolate and screen genes in the fungus, 626 expressed sequence tags(ESTs) were generated by a partial sequencing from the cDNA library. Paecilomyces tenuipes Jocheon-1 cDNA encoding the glyceraldehyde-3-phosphate dehydrogenase(Pt-GAPDH) of Paecilomyces tenuipes Jocheon-1 was cloned from the above cDNA library. The complete cDNA sequence of Pt-GAPDHis comprised of 1,014bp encoding 338 amino acid residues. The deduced protein sequence of Pt-GAPDH showed higher homology with Beauberia bassiana-GAPDH(93% amino acid identity). Hydropathy analysis revealed that Pt-GAPDH protein is hydrophilic. The major three amino acids in its composition of amino acid residues were alanine(11.54%), valine(9.47%) and glycine(8.88%). The cDNA encoding Pt-GAPDH was expressed as a 37 kDa polypeptide in baculovirus-infected insect Sf9 cells. The Pt-GAPDH gene of Paecilomyces tenuipes entomopathogenic fungus consisted of three exons and two introns coding for 338 amino acid residues, and the genomic DNA length of the gene spans 1302bp. The accession number of the gene in GenBank are GU997099 for Pt-GAPDH cDNA and GU997102 for Pt-GAPDH genomic DNA.
Nam Sook Park,Han Seok Kang,Seon Ku Kim,Yong Gyun Kim,Byung Uuk Cho,Teak Soon Shin,Keun Ki Kim,Hyun Chul Park,Hong Joo Son,Hong Gu Lee,Sang Mong Lee 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05
Fungi belonging to the Paecilomyces spp. have recently been used as food and herbal medicines in Korea and are greatly popular as commercially available powdered supplement or dried fruiting body. Despite this acceptance and its use, little is known of the genes related to its reactive agents. Presently, We have constructed an olig-d(T) primed directional cDNA library from the silkworm Dongchunghacho, an entomopathogenic fungus, of which species is belonging to Paecilomyces spp. based on the previous identification of ITS1 and ITS2 at the molecular level and collected from Jocheon Miryang, Korea. To isolate and screen genes in the fungus, 626 expressed sequence tags(ESTs) were generated by a partial sequencing from the cDNA library. cDNA encoding the glyceraldehyde-3-phosphate dehydrogenase(Pt-GAPDH) of Paecilomyces tenuipes- Jocheon was cloned from the above cDNA library. The complete cDNA sequence of Pt-GAPDH is comprised of 1,014bp encoding 338 amino acid residues. The deduced protein sequence of Pt-GAPDH showed higher homology with Beauberia bassiana-GAPDH(93% amino acid identity). Hydropathy analysis revealed that Pt-GAPDH protein is hydrophilic. The major three amino acids in its composition of amino acid residues were alanine(11.54%), valine(9.47%) and glycine(8.88%). The Pt-GAPDH gene of Paecilomyces tenuipes entomopathogenic fungus consisted of three exons and two introns coding for 338 amino acid residues, and the genomic DNA length of the gene spans 1302bp. The accession number of the gene in GenBank are GU997099 for Pt-GAPDH cDNA and GU997102 for Pt-GAPDH genomic DNA. More investigation works including gene expression, immunological analysis etc. will be carried continuously without hesitation after this presentation.
Sang Gu KANG,Kyung Eun LEE,Mahendra SINGH,Ramachandran VINAYAGAM 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Coronavirus diease-19 (COVID-19) pandemic, cuasued by the novel SARS-CoV-2 virus continue tobe a global threat. The number of cases and deaths will remain escalating due to the lack of effective therapeutic agents. Several studies have established the importance of the viral main protease (Mpro) in the replication of SARS-CoV-2 which makes it an attractive target for antiviral drug development, including pharmaceutical repurposing and other medicinal chemistry approaches. Identification of natural products with considerable inhibitory potential against SARS-CoV-2 could be beneficial as a rapid and potent alternative with drug-likeness by comparison to de novo antiviral drug discovery approaches. Thereof, we carried out the structure-based screening of natural products from Echinacea-angustifolia, commonly used to prevent cold and other microbial respiratory infections, targeting SARS-CoV-2 M<SUP>pro</SUP>. Four natural products namely, Echinacoside, Quercetagetin 7-Glucoside, Levan N, Inulin from chicory, 1,3-Dicaffeoylquinic Acid, revealed significant docking energy (>10 ㎉/㏖) in the SARS-CoV-2 M<SUP>pro</SUP> catalytic pocket via substantial inter㏖ecular contacts formation against cocrystallized ligand (< 4 ㎉/㏖). Furthermore, the docked poses of SARS-CoV-2 M<SUP>pro</SUP> with selected natural products showed conformational stability through ㏖ecular dynamics. Exploring the end-point net binding energy exhibited substantial contribution of Coulomb and van der Waals interactions to the stability of respective docked conformations. These results advocated the natural products from Echinacea angustifolia for further experimental studies with an elevated probability to discover the potent SARS-CoV-2 Mpro antagonist with higher affinity and drug-likeness.
A Black Metal-dielectric Thin Film for High-contrast Displays
Sang-Hwan Cho,Min-Kyo Seo,강주형,Jin-Kyu Yang,Sung-Yong Kang,KyuHwan Hwang,ByoungDuk Lee,Joon-Gu Lee,Young-Woo Song,JongHyuk Lee,이용희 한국물리학회 2009 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.55 No.2
We demonstrate a thin metal-dielectric structure that delivers low reflection and high absorption over the entire visible spectrum. The thin black film consists of SiO2/Cr/SiO2/Al layers deposited on a glass substrate. The measured reflectance and absorptance of the black film are 0.7% and 99.3%, respectively, when averaged over the range 380 – 780 nm. The total thickness of the black film is only about 220 nm, and it can be used as a thin absorbing layer for displays that require both broadband antireflection and high-contrast characteristics.
Morphological Characters of Panicle and Seed Mutants of Rice
Kang Sang-Gu,Pandeya Devendra,Kim Sung-Soo,Suh Hak-Soo The Korean Society of Crop Science 2006 Korean journal of crop science Vol.51 No.4
Phenotypes of panicle, hull and seed of mutant rice (Oryza sativa L.) were characterized. Panicle mutants were classified in 4 groups with their internode length of main rachis, primary rachis, secondary rachis and pedicel. Hull and seed mutants were grouped into 12 based on their mutant characters in shape, size and color of seeds. These natural and spontaneous mutant collections showed distinct phenotypes to wild type rice. This might be useful for the identification of the functions of genetic factors in the Mendelian inheritance.
$TiO_2$ Nanotubes Preparation and Its Formation Mechanism
Kang, Young-Gu,Shin, Ki-Seok,Ahn, Sung-Hwan,Hahm, Hyun-Sik The Korean Society of Applied Science and Technolo 2010 한국응용과학기술학회지 Vol.27 No.4
There has been a controversy on the formation mechanism of $TiO_2$ nanotubes. This study was conducted to elucidate the formation mechanism of $TiO_2$ nanotubes. $TiO_2$ nanotubes were prepared by a hydrothermal method. $TiO_2$ nanotubes formation mechanism was investigated by controlling the formation time. It was found that $TiO_2$ nanotubes were formed by growing, not by wrapping of sheets. The phase structure of hydrogen titanate nanotubes was different from that of $TiO_2$ nanotubes. It is important to wash the sodium titanate nanotubes with an acidic solution to get hydrogen titanate nanotubes and then to calcine the hydrogen titanate nanotubes around $400^{\circ}C$ to obtain $TiO_2$ nanotubes.