Comparative analysis of VMT genes/proteins in selected plant species with emphasis on bread wheat (Triticum aestivum L.)

Sharma Hemant,Shayaba,Kumar Rahul,Kumar Jitendra,Bhadana Deepa,Batra Ritu,Singh Rakhi,Kumar Sachin,Roy Joy K,Balyan Harindra S.,Gupta Pushpendra K. 한국유전학회 2023 Genes & Genomics Vol.45 No.11

Background In recent years, the study of molecular basis of uptake, transport and utilization of grain Fe/Zn (GFe/GZn) in wheat has been an active area of research. As a result, it has been shown that a number of transporters are involved in uptake and transport of Fe. In a recent study, knockout of a transporter gene OsVMT (VACUOLAR MUGINEIC ACID TRANSPORTER) in rice was shown to be involved in Fe homoeostasis. Objective In this study, we analysed VMT genes among six monocots and three dicots with major emphasis on wheat VMT genes (TaVMTs), taking OsVMT gene as a reference. Methods and results Using OsVMT gene as a reference, VMT genes were identified and sequence similarities were examined among six monocots and three dicots. Each VMT protein carried one functional domain and 7 to 10 distinct motifs (including 9 novel motifs). The qRT-PCR analysis showed differential expression by all the six TaVMT genes in pairs of contrasting wheat genotypes with high (FAR4 and WB02) and low (K8027 and HD3226) GFe/GZn at two different grain filling stages (14 DAA and 28 DAA). TaVMT1 genes showed up-regulation in high GFe/Zn genotypes relative to low GFe/Zn genotypes, whereas the TaVMT2 genes showed down-regulation or nonsignificant up-regulation in a few cases. Conclusions At 14 DAA, each of the six TaVMT genes exhibited higher expression in wheat genotypes with high GFe and GZn relative to those with low GFe and GZn, suggesting major role of VMT genes in improvement of grain Fe/Zn homoeostasis, thus making TaVMT genes useful for improvement in Fe/Zn in wheat grains.

Detection of Biomolecules Using Charge-Plasma Based Gate Underlap Dielectric Modulated Dopingless TFET

Sachin K. Verma,Shailendra Singh,Girish Wadhwa,Balwinder Raj 한국전기전자재료학회 2020 Transactions on Electrical and Electronic Material Vol.21 No.5

In this Paper, Dielectric Modulated Dopingless Double Gate Transistor (DM-DLDGTFET) device is proposed for the free label detection of the charged and neutral biomolecules. A charge-plasma principle is used for label-free detection of biosensors to reduce the processing complexity and cost of nanoscale products. Firstly, the simulations for the proposed device are carried using Atlas and diff erent electrical parameters are analyzed using the same. It is observed that the dielectric constant and diff erent biomolecule charges for example, protein, DNA, enzyme, cell and many more molecules aff ects the electrical characteristic of the device. The deposition of diff erent workfunction materials over silicon body will do the formation of p+ source and n+ drain region in the DM-DLDGTFET. In addition, by etching the segment of the gate oxide layer to the source end for sensing biomolecules, a nano gap cavity is embedded within the dielectric gate. When biomolecule get immobilized at cavity region, the electrostatic properties of device for example, drain current, I on /I off , subthreshold slope, average subthreshold slope, sensitivity get infl uenced. The energy band diagram and the device’s surface potential for both neutral and charged biomolecules are also discussed. For the validation of the proposed DL-TFET structure, the simulation results are calibrated with reported results and signifi cant improvement are observed in the proposed structure.

Sensitivity Evaluation of a Multi-Layered Heterostructure Blue Phosphorene/MoS 2 Surface Plasmon Resonance Based Fiber Optic Sensor: A Simulation Study

Sachin Singh,A. K. Sharma,Pooja Lohia,D. K. Dwivedi 한국전기전자재료학회 2022 Transactions on Electrical and Electronic Material Vol.23 No.3

In this study, an angular interrogation technique has been used for modeling a highly sensitive surface plasmon resonance (SPR)based biosensor. The large surface area of the heterostructure of the blue phosphorene (BP/MoS 2 ) layer facilitates the biomolecules absorption. A four-layer Kretschmann model of the SPR biosensor containing the BP/MoS 2 heterostructure with a gold layer is proposed. Compared to the traditional gold fi lmbased SPR biosensors, the sensitivity of the proposed SPR biosensor has been signifi cantly improved. An enhanced sensitivity 224.57°/RIU has been achieved by optimizing the proposed structure with 50 nm thick gold layer and a monolayer of heterostructure BP/MoS 2 with a thickness of 0.75 nm. Moreover, the proposed BP/MoS 2 heterostructure off ers extremely small FWHM, high detection accuracy, and highquality factor parameters. The highest sensitivity of 252°/RIU was found with two-layers of BP/MoS 2 heterostructure confi guration. It is observed that, compared to previously reported sensitivity, the proposed SPR biosensor shows better results.

Effect of temperature on elastic, mechanical and thermophysical properties of VNx (0.76 ≤ x ≤ 1.00) epitaxial layers

Rai Sachin,Prajapati Aadesh K.,Yadawa Pramod K. 한국물리학회 2023 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.82 No.1

The efect of temperature on the elastic, thermophysical and mechanical properties of VNx (0.76≤x≤1.00) epitaxial layers has been investigated using Coulomb and Born–Mayer potentials approach up to second nearest neighbor. The elastic constants and its allied properties (Bulk, Shear, Poisson's ratio and Young’s modulus) are analyzed to provide insights into the mechanical behavior of VNx (0.76≤x≤1.00) transition-metal nitrides using second-order elastic constants (SOECs). SOECs are used to measure the temperature deviation of ultrasonic velocities along the crystal’s z axis. The temperature variation of Debye average velocity and thermal relaxation time are calculated along same orientation. Thermal conductivity has been observed to be the main contributing factor to the temperature-dependent behavior of ultrasonic attenuations with phonon–phonon interaction. Depending on G/B ratios, the nitride groups are attributed to brittle and ductile behavior in the future. Including all epitaxial layers, the estimated melting temperatures are in better agreement with those calculated with B and C11 and have highest strength and highest harness. Transition metal nitrides have unusual chemical and physical properties, which are commonly used in industrial felds that require working at temperatures.

Synthesis of cross-linked protein-metal hybrid nanoflowers and its application in repeated batch decolorization of synthetic dyes

Patel, Sanjay K.S.,Otari, Sachin V.,Li, Jinglin,Kim, Dong Rip,Kim, Sun Chang,Cho, Byung-Kwan,Kalia, Vipin C.,Kang, Yun Chan,Lee, Jung-Kul Elsevier 2018 Journal of hazardous materials Vol.347 No.-

<P><B>Abstract</B></P> <P>Herein, we report the preparation of a cross-linked protein-metal hybrid nanoflower (NF) system for laccase immobilization. The immobilized laccase showed effective encapsulation yield and activity recovery of 78.1% and 204%, respectively. The catalytic efficiency (<I>k</I> <SUB>cat</SUB> <I>V</I> <SUB>max</SUB> <SUP>−1</SUP>) of cross-linked NF (CL-NF) was 2.2-fold more than that of free laccase. The CL-NF also exhibited significantly higher stability towards pH and temperature changes. It exhibited excellent storage stability and tolerance towards solvents and inhibitors as compared with the free enzyme. After 10 cycles of reuses, the NF and CL-NF laccase showed 41.2% and 92.3% residual activity, respectively. The CL-NF showed high oxidation potential, 265% that of the free enzyme, towards phenolic compounds. The CL-NF laccase retained the residual decolorization efficiency of up to 84.6% for synthetic dyes under repeated batch conditions of 10 cycles. These results suggested that the preparation of CL-NF is an effective approach to enhance the enzymatic properties and has great potential in many industrial applications.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Cross-linked (CL) laccase-metal hybrid nanoflower (NF) was prepared. </LI> <LI> The catalytic efficiency of CL-NF laccase was 2.2-fold higher than that of free laccase. </LI> <LI> CL-NF laccase showed 2.6-fold higher oxidation potential than free laccase towards phenolic compounds. </LI> <LI> Under repeated batch conditions, it retained high decolorization efficiency for synthetic dyes. </LI> </UL> </P>

Taxonomic notes on fruit flies of tribes Schistopterini and Eutretini (Diptera: Tephritidae: Tephritinae) in India, with description of a new species of Rhabdochaeta de Meijere

David Karamankodu Jacob,Hancock David Lawrence,Sachin K.,Gracy Gandhi R,Swathi R.S. 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.1

Rhabdochaeta nigroapicalis David, Hancock and Sachin, sp. n., is described from Assam, NE India. A key to all species of Schistopterini and Eutretini recorded from India is provided and taxonomic notes on previously described species are included. DNA barcode sequences of Calloptera asteria (Schiner), Rhabdochaeta pulchella de Meijere, Rhabdochaeta nigroapicalis David, Hancock and Sachin, sp. n. and Rhochmopterum venustum (de Meijere) were obtained and reported. Phylogenetic analysis using 62 mtCOI sequences of Tephritinae revealed Schis topterini to be a monophyletic group and the new species closely related to Rhabdochaeta pulchella, justifying its placement in Rhabdochaeta de Meijere. http://zoobank.org/A97337B3-E2BD-460D-BA62-6D3D4A1A60A2

Improving Contrast in Random Grids Based Visual Secret Sharing

Sachin Kumar,R. K. Sharma 보안공학연구지원센터 2012 International Journal of Security and Its Applicat Vol.6 No.1

SOLUTION TO INVERSE PROBLEM INVOLVING TIMOSHENKO SHAFT/BEAM USING COMPLIANCE APPROCH.

Sachin S. Naik,Surjya K. Maiti 한국소음진동공학회 2008 한국소음진동공학회 국제학술대회논문집 Vol.2008 No.7

Numerical and Experimental Study on Effect of Different Types of Field-Shaper on Electromagnetic Terminal-Wire Crimping Process

Ashish K. Rajak,Ramesh Kumar,Hirak Basumatary,Sachin D. Kore 한국정밀공학회 2018 International Journal of Precision Engineering and Vol.19 No.3

Electromagnetic terminal-wire crimping (EMTWC) is a type of mechanical joining process. In EMTWC, the terminal is deformed over the wire strands plastically to enhance the mechanical strength by increasing the contact area. A well-crimped electrical interconnection helps to avoid harmful configurations by reducing sparks, overheating, power losses etc. Subsequently, the challenge to minimize various disadvantages of conventional mechanical terminal-wire crimping process like non-uniform deformation, decreased durability, cracks, etc., can be overcome by the electromagnetic crimping process. In this work, application of the fieldshaper has been proposed to concentrate the magnetic pressure at the desired location for effective wire crimping. Numerical analysis was carried out using LS-DYNATM on three different types of field-shaper namely single-step, double-step and tapered. In all the three field-shaper, the total length, the outer diameter and the effective working length were constant. The samples used was an aluminum terminal and seven strands of aluminum wires which are commercially used for making electrical inter connections. Experiments were carried out based on the results obtained through numerical analysis. Experimental and numerical results were in agreement and the error was less than 10%. The efficiency of single-step field-shaper was found to be better compared to double-step and tapered fieldshaper.

Designing of field shaper for the electro-magnetic crimping process

Ashish K Rajak,Ramesh Kumar,Sachin D Kore 대한기계학회 2019 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.33 No.11

Design of a field shaper plays an essential role in the electromagnetic crimping process. It needs to be designed in such a manner that maximum magnetic field is concentrated at the active working zone. Simultaneously it is essential to take care of the mechanical strength of the field shaper so that the von Mises stress acting over the field shaper should always be lower than the strength of the material used for making field shaper. In this manuscript, numerical and experimental work is carried out in the variation of the effective height of the field shaper, to get the most suitable effective working zone height for maximum magnetic pressure acting over the workpiece. Research is carried out over the electrical interconnectors as an application, to achieve maximum crimping of the terminal over the wire strands. Results like the current density, Von-Mises stress, magnetic field, Lorentz force, terminal deformation, and contact length are discussed in detail. These results will help to design a field shaper for different industrial applications.