Molecular mechanisms conferring asymmetrical cross-resistance between tebufenozide and abamectin in Plutella xylostella

Qian Yin,Lu Qian,Pingping Song,Tunyu Jian,Zhao-Jun Han 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.1

Based on the confirmation of asymmetrical cross-resistance between abamectin and tebufenozide in Plutella xylostella, the present work proved that the cytochrome P450 monooxygenase plays a decisive role in crossresistance, and the expression of various cytochrome P450 (CYP450) genes in different strains was surveyed to elucidate the molecular basis of the underlying mechanisms. Enzyme analysis showed the activity of cytochrome P450 monooxygenase was notable enhanced in the strains resistant to both tebufenozide (3.07-fold) and abamectin (3.37-fold), suggesting that the enhancement of cytochrome P450 monooxygenase is the main detoxification mechanism responsible for the cross-resistance. CYP4M7 (64.58-fold) and CYP6K1 (41.97-fold) had extremely high expression levels in the Teb-R strain, selected using tebufenozide, which was highly resistant to tebufenozide (RR 185.5) and moderately cross-resistant to abamectin (RR 41.0). When this strain was subjected to further selection using abamectin, the resultant Aba-R strain showed a higher expression of CYP6K1 (60.32-fold). However, the expression of CYP4M7 was reduced (10.62-fold). Correspondingly, the Aba-R strain became more resistant to abamectin (RR 593.8) and less resistant to tebufenozide (RR 28.0). Therefore, we concluded that the over expression of CYP4M7 was the main cause for tebufenozide resistance, and that CYP6K1 mainly conferred abamectin resistance. The asymmetrical cross-resistance occurred because tebufenozide selection not only enhanced the expression of CYP4M7, but also that of CYP6K1. This is the first report on the molecular mechanism of asymmetrical cross-resistance between insecticides.

Investigation on mechanical behavior and crack coalescence of sandstone specimens containing fissure-hole combined flaws under uniaxial compression

Qian Yin,Hongwen Jing,Haijian Su 한국지질과학협의회 2018 Geosciences Journal Vol.22 No.5

This study focuses on the effect of pre-existing flaw geometry on mechanical behavior and crack coalescence modes of sandstone specimens containing combined flaws with different fissure angle, ligament length and fissure length under uniaxial compression. The flaw geometry is a combination of a single hole and an inclined fissure underneath, which is generated by a high pressure water-jet cutting machine and is different from that reported in previous studies. The effect of flaw geometry on mechanical behavior of sandstone specimens is analyzed. Basically, mechanical parameters including the peak strength, peak axial strain, elastic modulus and secant Young’s modulus for the flawed specimens are lower than those for the intact specimens, with the reduction extent related to the fissure angle, ligament length and fissure length. Variation trends of the crack initiation stress for all tested cases are studied. Initiated crack types and cracking modes also depend on the combined flaws geometry. For the flawed specimens with a small fissure angle, ligament length or fissure length, cracking modes are generally characterized by cracks initiated from the hole-wall and evolved to the specimen boundary. However, when the fissure angle, ligament length or fissure length is increased, cracks initiated from both the hole-wall and fissure tips produce the main failure planes, accompanied by a free-standing “triangular prism structure” within the specimens. Numerical simulations using RFPA2D (Rock Failure Process Analysis in two dimensions) are carried out on the flawed sandstone specimens and agree well with the experimental results in the peak strength and overall cracking behavior.

Alkylation of phenol with tert-butyl alcohol over zirconium incorporated mesoporous molecular sieves with enhanced acidity

Qian Zhao,Qian Wang,Duolin Wu,Xiaoqi Fu,Tingshun Jiang,Hengbo Yin 한국공업화학회 2012 Journal of Industrial and Engineering Chemistry Vol.18 No.4

Several SO42-/Zr-MCM-41 and SO42-/Zr-MCM-48 solid acid catalysts were prepared by wet impregnation method and were characterized by FT-IR, XRD, NH3-TPD and N2 physical adsorption,respectively. The catalytic activities of these catalysts were evaluated in alkylation reaction of phenol and tert-butyl alcohol. Experimental results reveal that the four solid acid catalysts still maintained a typical mesoporous framework. Under comparable condition, the SO42-/Zr-MCM-48-25 catalyst exhibits much high catalytic activity and gives the highest phenol conversion as compared with the other catalysts. A maximum phenol conversion of 91.6% with 81.8% selectivity to 4-tert-butylphenol (4-TBP) was achieved at the reaction temperature of 140 8C. 2012 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.

Characterization and synthesis of Ce-incorporated mesoporous molecular sieves under microwave irradiation condition

Qian Zhao,Qian Wang,Yajing Tang,Tingshun Jiang,Chang-sheng Li,Hengbo Yin 한국화학공학회 2010 Korean Journal of Chemical Engineering Vol.27 No.4

Ce-incorporated MCM-41 mesoporous molecular sieves (CeMCM-41) were synthesized by microwave irradiation method from sodium silicate and ammonium cerium (IV) nitrate precursors and using cetyltrimethyl ammonium bromide (CTAB) as template. The resulting samples were characterized by means of XRD, TEM, FT-IR, UV-Vis,XPS and N2 physical adsorption, respectively. The effect of the Si/Ce molar ratio on the textural properties of CeMCM-41 was investigated. The results reveal that the CeMCM-41 was successfully synthesized. The resultant mesoporous materials have specific surface areas in the range of 602-1,216 m2/g and pore sizes in the range of ca. 2.6-2.9 nm. The structural properties are related to the amount of cerium incorporation. The surface area and pore volume of the resulting CeMCM-41 were gradually reduced as the cerium content in the sample increased, and the mesoporous ordering diminished.

High Temperature Deformation Behavior and Processing Map for a Nickel-Titanium Shape Memory Alloy

Xiang-Qian Yin,이상원,Yan-Feng Li,박찬희,Xu-Jun Mi,염종택 대한금속·재료학회 2017 METALS AND MATERIALS International Vol.23 No.5

The hot deformation behavior of 49.2Ti-50.8Ni shape memory alloy was studied using hot compressive deformationtesting in the temperature range of 1023-1323 K and at strain rates of 0.01-10 s-1. The work-hardening rate wasinduced to analyze the stress-strain curves, and the critical stress σc and the dynamic recovery saturation stress σsatwere measured which can be specified approximately by the expressions: σsat-1.12σp and σc-0.86σp. An Arrheniusmodel was calculated to describe the relationship between peak stress and the Z parameter. The relationshipbetween deformation activation energy, the deformation conditions and the effect of Ni component in a binaryTiNi alloy on the activation energy were discussed in this work. With the help of electron backscattering diffraction,a connected mode dynamic recrystallization microstructure was confirmed in peak efficiency regimes(850 °C & 0.01 s-1 and 1050 °C & 10 s-1) of the processing map.

Heteromerization of μ-opioid receptor and cholecystokinin B receptor through the third transmembrane domain of the μ-opioid receptor contributes to the anti-opioid effects of cholecystokinin octapeptide

Yin Yang,Qian Li,Qi-Hua He,Ji-Sheng Han,Li Su,You Wan 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Activation of the cholecystokinin type B receptor (CCKBR) by cholecystokinin octapeptide (CCK-8) inhibits opioid analgesia. Chronic opiate treatment leads to an increase in the CCK-8 concentration and thus enhances the antagonism of CCK-8 against opioid analgesia; the underlying molecular mechanisms remain of great interest. In the present study, we validated the colocalization of the μ-opioid receptor (MOR) and CCKBR in pain signal transmissionrelated spinal cord dorsal horn and dorsal root ganglion neurons of rats. Co-immunoprecipitation (Co-IP) and fluorescence lifetime-imaging-microscopy-based fluorescence resonance energy transfer (FLIM-FRET) assays showed that MOR heteromerized with CCKBR directly in transfected HEK293 cells. Combined with MOR mutant construction, the third transmembrane domain of MOR (TM3MOR) was demonstrated to participate in heteromerization with CCKBR. Receptor ligand binding, ERK phosphorylation and cAMP assays showed that MOR heteromerization with CCKBR weakened the activity of MOR. A cell-penetrating interfering peptide consisting of TM3MOR and TAT (a transactivator of HIV-1) sequences from the N terminal to the C terminal disrupted the MOR–CCKBR interaction and restored the activity of MOR in transfected HEK293 cells. Furthermore, intrathecal application of the TM3MOR-TAT peptide alleviated CCK-8-injection-induced antagonism to morphine analgesia in rats. These results suggest a new molecular mechanism for CCK-8 antagonism to opioid analgesia in terms of G-protein-coupled receptor (GPCR) interaction through direct heteromerization. Our study may provide a potential strategy for pain management with opioid analgesics.

A MEMORY-ATTENTION HIERARCHICAL MODEL FOR DRIVING-BEHAVIOR RECOGNITION AND MOTION PREDICTION

Yin Huilin,Wang Jie,Lin Jia,Han Daguang,Ying Chunli,Meng Qian 한국자동차공학회 2021 International journal of automotive technology Vol.22 No.4

Proper understanding and prediction of driving behavior of surrounding vehicles are one of the most significant requirements for automated driving especially when it comes to safety on a highway. In this paper, we propose a two-layer memory-attention hierarchical model (MAHM) for driving-behavior recognition and motion prediction. This model is based on the human driver’s thinking as well as on brain physiology, i.e., working memory and the selective-attention mechanism. The first layer is a hidden Markov model (HMM), which is used to achieve efficient recognition of driving behavior. The second layer is a memory-attention recurrent neural network (MARNN) for motion prediction, which derives the data from vehicles of interest as input according to driving behavior. Finally, the experimental analysis is performed on the real-data NGSIM US-101 and HighD datasets for highway-driving scenes. We report our results from three perspectives: accuracy of driving-behavior classification, error of predicted trajectories, and execution time.

The role of the apoptosis-related protein BCL-B in the regulation of mitophagy in hepatic stellate cells during the regression of liver fibrosis

Qian Ding,Xiao-Li Xie,Miao-Miao Wang,Jie Yin,Jin-Mei Tian,Xiao-Yu Jiang,Di Zhang,Jing Han,Yun Bai,Zi-Jin Cui,Hui-Qing Jiang 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

The clearance of activated hepatic stellate cells (HSCs) by apoptosis is critical for the reversibility of hepatic fibrosis. Mitochondrial homeostasis is regulated by mitophagy, which is an efficient way of clearing injured mitochondria that plays an important role in apoptosis. However, the role of mitophagy in apoptosis in HSCs and hepatic fibrosis is still unclear. Here, we show that mitophagy is enhanced in parallel with increased apoptosis in hepatic stellate cells during the reversal of hepatic fibrosis. The inhibition of mitophagy suppressed apoptosis in HSCs and aggravated hepatic fibrosis in mice. In contrast, the activation of mitophagy induced apoptosis in HSCs. Furthermore, we confirmed that BCL-B, which is a member of the BCL-2 family, is a regulator mediating mitophagy-related apoptosis. The knockdown of BCL-B resulted in increased apoptosis and mitophagy in HSCs, while the overexpression of BCL-B caused the opposite effects. BCL-B inhibited the phosphorylation of Parkin (a key regulator of mitophagy) and directly bound phospho-Parkin. Altogether, enhanced mitophagy promotes apoptosis in HSCs during the reversal of hepatic fibrosis. BCL-B suppresses mitophagy in HSCs by binding and suppressing phospho-Parkin, thereby inhibiting apoptosis. BCLB- dependent mitophagy is a new pathway for the regulation of apoptosis in HSCs during the regression of hepatic fibrosis

Mixed-type inhibition of tyrosinase from Agaricus bisporus by terephthalic acid: computational simulations and kinetics.

Yin, Shang-Jun,Si, Yue-Xiu,Chen, Yong-Fu,Qian, Guo-Ying,L?, Zhi-Rong,Oh, Sangho,Lee, Jinhyuk,Lee, Sanghyuk,Yang, Jun-Mo,Lee, Dong-Youn,Park, Yong-Doo Kluwer Academic/Plenum 2011 The Protein Journal Vol.30 No.4

<P>Tyrosinase inhibition studies are needed due to the agricultural and medicinal applications. For probing effective inhibitors of tyrosinase, a combination of computational prediction and enzymatic assay via kinetics were important. We predicted the 3D structure of tyrosinase from Agaricus bisporus, used a docking algorithm to simulate binding between tyrosinase and terephthalic acid (TPA) and studied the reversible inhibition of tyrosinase by TPA. Simulation was successful (binding energies for Autodock4 = -1.54 and Fred2.0 = -3.19 kcal/mol), suggesting that TPA interacts with histidine residues that are known to bind with copper ions at the active site. TPA inhibited tyrosinase in a mixed-type manner with a K ( i ) = 11.01 2.12 mM. Measurements of intrinsic and ANS-binding fluorescences showed that TPA induced no changes in tertiary structure. The present study suggested that the strategy of predicting tyrosinase inhibition based on hydroxyl groups and orientation may prove useful for screening of potential tyrosinase inhibitors.</P>

Comparative transcriptome analysis to identify genes involved in terpenoid biosynthesis in Agriophyllum squarrosum, a folk medicinal herb native to Asian temperature deserts

Yin Xiaoyue,Yan-Xia Liu,Qian Chaoju,Zhou Shanshan,Fang Tingzhou,Fan Xingke,Gao yuan,Chang Yuxiao,Yang Jian,Ma Xiao-Fei 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.3

Agriophyllum squarrosum is a folk Mongolian medicine with pleiotropic pharmacological and ecological economic importance endemic to Asian temperature deserts. Terpenoids play critical roles in biotic and abiotic stresses due to their antioxidative activities. Based on non-targeted metabolomic analysis, we detected eight terpenoids enriched in the above-ground tissues of A. squarrosum, however, the molecular mechanism underlying terpenoids biosynthesis in this desert medicinal plant is rarely understood. Here, a comparative transcriptome analysis of diferent tissues in A. squarrosum was conducted to identify 84 unigenes encoding key enzymes in the upstream backbone biosynthesis and 53 unigenes encoding the downstream enzymes for terpenoid diversifcation. Most of the upstream genes exhibited signifcant high expression levels in leaf, and some of which were validated by qRT-PCR. Phylogenetic analysis showed that two downstream gene families OSCs (oxidosqualene cyclases) and TPSs (mainly in terpene synthases -g subfamily) had undergone notable gene expansions in A. squarrosum comparing with the other Amaranthaceae plant species and Arabidopsis. Nevertheless, most members from these two gene families showed the tissue-specifc expression in A. squarrosum, which supported the diversifcation and tissue-specifc enrichment of terpenoids across above-ground tissues. Considering to the habitat characteristics of A. squarrosum, we proposed that the enrichment of terpenoids and the functional diversifcation of terpenoids biosynthesis enzymes were more or less involved into its adaptation to stressful environments of deserts. These results expand the available genetic information underlying terpenoid biosynthesis in A. squarrosum, and contribute to deeper researches on pharmaceutical and eco-agricultural applications in this desert medicinal plant.