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Poudel Santosh(표우델 산토스),Shin Young-Suk(신영숙) 한국멀티미디어학회 2009 한국멀티미디어학회 학술발표논문집 Vol.2009 No.1
Biometric is an interesting research area where there are different approaches studied in the literature. This paper performs the comparison analysis of methods based Principal Components Analysis (PCA) and Linear Discriminate Analysis (LDA) for pattern recognition of human teeth. PCA and LDA are appearance based techniques, used widely for the dimensionality reduction and recorded a great performance for face recognition. To extract feature this paper uses the subspace created by the eigenvectors of the covariance matrix and the Fisher basis vectors. The training teeth images was mapped to the subspace for recognition, and the test teeth images are projected to the same subspace for recognition. The recognition measures as Euclidean distance to determine the class from training data to test data. Our experimental result was verified the superiority of the PCA method over the LDA algorithms in teeth recognition.
Poudel, Bijay Kumar,Gupta, Biki,Ramasamy, Thiruganesh,Thapa, Raj Kumar,Pathak, Shiva,Oh, Kyung Taek,Jeong, Jee-Heon,Choi, Han-Gon,Yong, Chul Soon,Kim, Jong Oh Elsevier 2017 Colloids and surfaces Biointerfaces Vol.160 No.-
<P><B>Abstract</B></P> <P>Pancreatic cancer has extremely poor prognosis with an 85% mortality rate that results from aggressive and asymptomatic growth, high metastatic potential, and rapid development of resistance to already ineffective chemotherapy. In this study, plasmonic hollow gold nanoshells (GNS) coated with PEGylated thermosensitive lipids were prepared as an efficient platform to ratiometrically co-deliver two drugs, bortezomib and gemcitabine (GNS-L/GB), for combinational chemotherapy and photothermal therapy of pancreatic cancer. Bortezomib was loaded within the lipid bilayers, while gemcitabine was loaded into the hydrophilic interior of the porous GNS via an ammonium sulfate-driven pH gradient method. Physicochemical characterizations and biological studies of GNS-L/GB were performed, with the latter using cytotoxicity assays, cellular uptake and apoptosis assays, live/dead assays, and western blot analysis of pancreatic cancer cell lines (MIA PaCa-2 and PANC-1). The nanoshells showed remotely controllable drug release when exposed to near-infrared laser for site-specific delivery. GNS-L/GB showed synergistic cytotoxicity and improved internalization by cancer cells. High-powered near-infrared continuous wave laser (λ=808nm) effectively killed cancer cells via the photothermal effect of GNS-L/GB, irrespective of cell type in a power density-, time-, and GNS dose-dependent manner. These results suggest that this method can provide a novel approach to achieve synergistic combinational chemotherapy and photothermal therapy, even with resistant pancreatic cancer.</P> <P><B>Highlights</B></P> <P> <UL> <LI> PEGylated thermosensitive lipid-coated hollow gold nanoshells (GNS-L) were prepared. </LI> <LI> GNS-L were co-loaded with hydrophilic gemcitabine and hydrophobic bortezomib. </LI> <LI> NIR irradiation induced drug release for remotely controlled site-specific delivery. </LI> <LI> GNS-L increased cellular uptake and apoptosis in pancreatic cancer cells. </LI> <LI> Direct photothermal killing of cancer cells was observed on NIR laser irradiation. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Poudel, Barun,Ki, Hyeon-Hui,Luyen, Bui Thi Thuy,Lee, Young-Mi,Kim, Young-Ho,Kim, Dae-Ki Oxford University Press 2016 Acta biochimica et biophysica Sinica Vol.48 No.2
<P>Non-small cell lung cancer (NSCLC) is the major cancer-related death worldwide with only 14% five-year survival rate. Triticumoside, a phenolic compound present in Triticum aestivum sprout extract, has been recognized to have antiobesity and anti-inflammatory effects. However, the effect of triticumoside on cancer cell proliferation and migration has not been studied. In order to elucidate whether triticumoside exhibits an anticancer effect, cells were incubated with different doses of triticumoside, and apoptosis was assessed by observing cell viability, cellular morphological changes, and annexin-V-fluorescein isothiocyanate/propidium iodide staining. Cell cycle analysis, western blotting, wound healing assay, and quantitative-polymerase chain reaction were also performed. Triticumoside exhibited marked cytotoxicity in the cells in dose-and time-dependent manner. Triticumoside caused morphological changes, including cellular rounding, nuclear condensation, and shrinkage. Likewise, triticumoside enhanced the sub-G1 proportion of cells. Additionally, triticumoside regulated expression of apoptosis-associated proteins, such as B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X, and procaspase-3/9. Triticumoside also inhibited migration of the cells through downregulation of matrix metalloproteinase-2/9 (MMP2/9). Collectively, these results suggest that triticumoside induces apoptosis through caspase-dependent mitochondrial pathway and suppresses migration via inhibition of MMP2/9 in NSCLC A549 cells.</P>
Poudel, Sher Bahadur,Bhattarai, Govinda,Kook, Sung-Ho,Shin, Yun-Ji,Kwon, Tae-Ho,Lee, Seung-Youp,Lee, Jeong-Chae Elsevier 2017 Growth hormone & IGF research Vol.36 No.-
<P><B>Abstract</B></P> <P>Transgenic plant cell suspension culture systems have been utilized extensively as convenient and efficient expression systems for the production of recombinant human growth factors. We produced insulin-like growth factor-1 using a plant suspension culture system (p-IGF-1) and explored its effect on new bone formation in calvarial defects. We also compared the bone regenerating potential of p-IGF-1 with commercial IGF-1 derived from <I>Escherichia coli</I> (e-IGF-1). Male C57BL/6 mice underwent calvarial defect surgery, and the defects were loaded with absorbable collagen sponge (ACS) only (ACS group) or ACS impregnated with 13μg of p-IGF-1 (p-IGF-1 group) or e-IGF-1 (e-IGF-1 group). The sham group did not receive any treatment with ACS or IGFs after surgery. Live μCT and histological analyses showed critical-sized bone defects in the sham group, whereas greater bone formation was observed in the p-IGF-1 and e-IGF-1 groups than the ACS group both 5 and 10weeks after surgery. Bone mineral density, bone volume, and bone surface values were also higher in the IGF groups than in the ACS group. Local delivery of p-IGF-1 or e-IGF-1 more greatly enhanced the expression of osteoblast-specific markers, but inhibited osteoclast formation, in newly formed bone compared with ACS control group. Specifically, p-IGF-1 treatment induced higher expression of alkaline phosphatase, osteocalcin, and osteopontin in the defect site than did e-IGF-1. Furthermore, treatment with p-IGF-1, but not e-IGF-1, increased mineralization of MC3T3-E1 cells, with the attendant upregulation of osteogenic marker genes. Collectively, our findings suggest the potential of p-IGF-1 in promoting the processes required for bone regeneration.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We explored bone regenerating potential of IGF-1 produced by a plant culture system. </LI> <LI> Local delivery of IGF-1 increased bone formation in calvarial defect model of mice. </LI> <LI> IGF-1 treatment stimulated the expression of osteogenic marker genes in the defect. </LI> <LI> The IGF-1 induced new bone formation similar to that did a commercial IGF-1. </LI> <LI> These results support a clinical usefulness of the IGF-1 in repairing bone defects. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Poudel, Tej Narayan,Khanal, Hari Datta,Lee, Yong Rok The Royal Society of Chemistry 2018 NEW JOURNAL OF CHEMISTRY Vol.42 No.6
<P>An unprecedented methodology for the preparation of diverse <I>S</I>-alkyl 2-aminoarylthioates has been developed based on cesium carbonate-promoted direct ring opening of 3-chlorooxindoles with thiols. This novel protocol proceeds <I>via</I> cascade sulfenylation, aerial oxidation, C2-C3 bond cleavage, and decarboxylation. The resulting products from this transformation exhibit potential for application as fluorescent sensors for the detection of Fe<SUP>3+</SUP> ions. In addition, this methodology provides a concise pathway for the construction of a variety of biologically interesting quinazolin-4(3<I>H</I>)-ones in good yields.</P>
Liver Involvement in Multiple Myeloma: A Hospital Based Retrospective Study
Poudel, Bibek,Mittal, Ankush,Shrestha, Rojeet,Farooqui, Mohammad Shamim,Yadav, Naval Kishor,Shukla, Pramod Shanker Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5
Objective: This study was to assess liver involvement in multiple myeloma with the aid of liver function tests. Materials and Methods: A hospital based retrospective study was undertaken using data retrieved of multiple myeloma from the register maintained in the Department of Biochemistry of the Manipal Teaching Hospital, Pokhara, Nepal between $1^{st}$ January, 2007 and $28^{th}$ February, 2012. We collected biomarkers of liver profiles including bilirubin (Total, Direct and Indirect), total protein, albumin, AG ratio, SGOT, SGPT, ALP, ${\gamma}GT$, LDH, ferritin, renal profile and hematological profile. Descriptive statistics and testing of hypothesis were used for the analysis using EPI INFO and SPSS 16 software. Results: Out of 37 cases of multiple myeloma, serum level of AST, ALT, ALP, ${\gamma}GT$ and LDH were increased above the cut-off point in 22 (59.5%), 24 (64.86%), 13 (35.13%), 9 (24.3%) and 11 (29.7%) respectively. The mean values of AST ($65.5{\pm}28.18$ U/L), ALT ($68.37{\pm}29.74$ U/L), ALP ($328.0{\pm}148.4$ U/L), ${\gamma}GT$ ($44.5{\pm}29.6$ U/L) and LDH ($361.7{\pm}116.5$ U/L), total protein ($9.79{\pm}1.03$ gm/dl) were significantly increased when compared with controls. In contrast, albumin ($3.68{\pm}0.43$ gm/dl) and the AG ratio ($0.62{\pm}0.15$) were significantly decreased. Similarly, anemia, hyperuricemia, azotemia, hypercalcaemia and Bence Jones proteinuria were found in 30 (78.9%), 27 (71.1%), 19 (51.5%), 15 (39.5%) and 16 (42.1%) respectively, in cases of multiple myeloma. Conclusions: While clinical manifestation of liver disease among the multiple myeloma was not common, abnormalities in liver function were characteristic.