야생 버섯을 복용 후 발생한 재생불량성 빈혈 1예

조소영,곽명옥,김정아,나현희,황동희,이홍기 仁濟大學校 白病院 2010 仁濟醫學 Vol.31 No.-

Aplastic anemia is one of the hematologic disease which is characterized by cytopenia in peripheral blood, hypocelluarity in bone marrow and marrow failure. Most common cause of aplastic anemia is unknown. Acquired causes of it are viral infection, drugs, and chemical agents. The symptoms of aplastic anemia are fatigue, headache, dizziness, exertional dyspnea, and pallor, and they are due to abrupt decrease in hemoglobin level. The incidence of acquired aplastic anemia is higher in Eastern population than Western. In geeral, men and women are affected with equal frequency, but the age distribution is biphasic, with the major peak in the teens and tweenties and a second rise in the elderly. We report a case of a 28-year-old man who experience aplastic anemia after ingestion of Cordyceps with the review of literatures.

소나무로부터 배출되는 모노테르펜 구성비의 수령별 특성 연구

김조천,홍지형,주명칠,전진,김기준 동신대학교 환경연구소 2002 환경연구 Vol.7 No.1

소나무로부터 직접 배출되는 주요한 모노테르펜 물질로 α-pinene, camphene, myrcene, β -pinene, α-phellandrene, α-terpinene, d-limonene, p-cymene, β-phellandrene 등이 확인되었다. 7년생 소나무에서 직접 배출되는 모노테르펜 물질의 구성비를 분석한 결과 β-pinene이 67.0%로 가장 높았고, 22년생 소나무 경우에 β-phellanone이 37.4%로 가장 높았으며, 32년생 소나무는 α-pinene 구성비가 약 42.9%로 가장 높았다. 소나무는 수령에 따라서 구성비에 큰 차이를 보였으며, 소나무 수령이 높을수록 α-pinene이 구성비가 높았다. 소나무의 수령별 α-pinene과 β-pinene의 비율(α-pinene/β-pinene)을 비교한 결과, 7년생 소나무의 경우 0.16~l.15 범위로 나타났고, 22년생 소나무의 경우 0.64~2.47로 나타났으며, 32년생 소나무의 경우 0.50~18.47 범위로 나타났다. α-pinene과 β-pinene의 비는 수령이 높아질수록 좀 더 높은 값을 보였고, 동일한 수령일지라도 나무에 따라서 차이를 보였다. The major monoterpenes emitted from the Pinus densiflora were α-pinene, camphene, myrcene, β-pinene, α-phellandrene, α-terpinene, d-limonene, p-cymene and β-phellandrene. As a result of comparison of monoterpene composition by tree age for P. densiflora, it was found that β-pinene was a dominant monoterpene for 7-year-old pine(67%). β-Phellandrene(37.4%) and α-pinene(46.1%) were most abound for 22-year-old pine and 32-year-old pine respectively. The ratio of monoterpene composition varied considerably with tree age for the pine, and the percentage of α-pinene composition increased with tree age. The ratio of α-pinene and β-pinene(α-pinene/β-pinene) increased with tree age, and varied tree to tree.

특징형상개념을 이용한 CNC선반용 CAD/CAM시스템 개발

조진문,김석일,서명원 한국항공대학교 1995 論文集 Vol.33 No.-

Most of CAD/CAM systems for CNC lathe have been developed as the form of automatic programming systems which can generate autormatically or interactively the NC code from the process sheets and design drawings of products. But these systems have some problems during the modeling process of product shap since the shape is not described by the features intimate with system users such as chamfering, rounding, groove, arc and so on. Therfore, in this paer, the feature based CAD/CAM system for CNC lathe which is able to represent the truned product shapes by the typical features, is developed. Especially the system has the various capabilities such as the automatic determination of cutting domains, the automatic generation of CL data and NCcode, the real time cutting simulation for verification of NC code, the DNC operation and so on.

고분자 전해질 감습막의 합성과 전기적 성질

공명선,조준상,이학민,김경호 경북대학교 센서기술연구소 1991 센서技術學術大會論文集 Vol.2 No.1

고분자 습도센서의 감습재료로 이용하고자 4-vinylpyridine (4-VP) 과 methylmethacrylate(MMA) 의 공중함체를 전극에 도포한 후 α, ω-dihaloalkane으로 4 차 암모늄화시킨 후 습도변화에 따른 전기저항을 측정한 결과 소수성 모노머인 MMA의 양이증가할 수록 전기저항이 증가하였으며, 또한 α, ω-dihaloalkane에 의한 4차 암모늄화 반응시간에 따라서도 전기저항의 크기 및 감습막의 안정성에 차이가 있음을 알 수 있었다. In order to for a humidity sensitive material of polymeric humidity sensor, a copolymer of 4-vinylpyridine (4-VP) and methylmethacrylate (IBA) is coated on the electrode and then 4 quartermization by α, ω-dihaloalkane, and thereafter measured the electrical resistance due to humidity variation. As a result, the more content of MMA which is a hydrophobic monomer is increased, the more property of electrical resistance is increased. Also, according to the reaction time in 4 quarternization by α, ω-dihaloalkane we can see the diferences of the magnitude of electrical resistance and the stability of humidity -sensitive membrane.

이중외피 파사드의 운전모델 개발에 관한 연구

박유영,조재훈,여명석,김광우 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1

The objective of this study is to develop the operation model of the double-skin facade for energy conservation and user comfort. This study proposes development process of the operation model in three steps. A case study is used to investigate the feasibility of the process. The G building in Kungkido is chosen. The main contents of this study are as follows. 1) The operation objectives and standards are established. And the operation factors classified according to the objectives and standards. 2) Correlation of the operation factors are clarified. And Individual models. The operation model is essential to building which has the double-skin facade and logical operation would be improved by this model. For the practical application of this operation model, it will be needed to connect the building management system.

파라콰트 중독에서의 항산화치료

권운용,조유환,송형곤,김명천,이중의,서길준,윤여규 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4

Background: Paraquat causes severe tissue toxicity when ingested, but has no effective treatment modality. We have shown that high dose vitamin C has effective antioxidant activities against the paraquat intoxication in a previous animal experiment. This study was designed to evaluate the effect of antioxidant therapy with high dose vitamin C and vitamin E in human cases of paraquat intoxication. Methods: From August 1999 to August 2001, 19 paraquat intoxication patients who visited the emergency department of the Seoul National University Hospital and the Kyounghee University Hospital were enrolled to this study. They were devided into two groups, a control group(9 patients) and a study group(10 patients). The control group received only conservative managements including gastro-intestinal decontaminati-on. The study group received conservative managements plus the antioxidant therapy which was composed of vitamin C 24 gm/day intravenously and 20 gm/day orally, and vitamin E 1.6 gm/day orally. Results: In the study group, 5 of 10 patients(50%) survived, but all patients of the control group died(p=0.003). There were no significant differences in age, sex, and usage of gastric lavage and activated charcoal between the two groups. Difference in ingested amount of paraquat between the two groups could not be analyzed due to the inexact and subjective measuring methods based on patients' histories. Conclusion: Antioxidant therapy with high dose vitamin C and vitamin E is effective in vival rate in paraquat intoxicated patients.

남창천과 삼향천에 분포한 주요 수생식물의 수질정화능

임병선,이점숙,조승원,양효식,김명화,곽애경 木浦大學校 沿岸環境硏究所 1995 沿岸環境硏究 Vol.12 No.1

The nutrient removal-capacity of four major dominant species distributed in Namchang-chon polluted with livestock wastewater and Samhyang-chon polluted with industrial and domestic wastewater were investigated. Water qualities and soil properties of three polluted sites were a quite different in concentration of nitrogen, SS and Ga. Dominant species in livestock wasetewater were Persicaria thunbergii, Zizania latiforia, Phragmites communis and Oenanthe japanica, those in industrial and domestic were Phragmites communis. Changes of NO₃-N, NH₄-N and P concentrations of culture solution are high in cultivor of Zizania latiforia and Oenanthe japanica. All four species removed NO₃-N most effectively. In experiment used directely polluted water from three areas ; all species from livestock wasetewater uptaked NO₃-N effectively. Phragmites communis from domestic, and Persicaria thunbergii and Oenanthe japanica from industrial and domestic wasetewater removed NH₄-N effectively. All species cultured in industrial waserewater removed P well, and Phragmites communis and Persicaria thunbergii cultured in domestic wastewater removed it moderately.

결핵균 30-kDa와 32-kDa 단백항원의 분리정제와 마우스 비장세포 증식에 미치는 영향

백태현,오명주,김화중,조은경,박정규,최대경 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.1

Identification and characterization of individual components of M.tuberculosis have long been a focus of research on tuberculosis. The 30-kDa and 32-kDa antigens are major constituents of M bovis BCG and M. tuberculosis culture fluids. Because 30-kDa and 32-kDa antigens are partially identical, these antigens are difficult to purify in large amounts by biochemical techniques. This study was performed to purify the 30-kDa and 32-kDa antigens complexes primarily purified by 50% ammonium sulfate precipitation, hydroxylapatite chromatography and Sephadex G-75 gel filtration. And then further purification for separation of the two antigens was accomplished on preparative isoelectric focusing. Recovery of 30-kDa and 32-kDa antigens during above the purification procedures were 28% and 14%, respectively, and 147.0 and 59.8-fold purification were showed, respectively. On silver stained SDS-PAGE gels, the purified 32-kDa antigen gave a single band at 32-kDa molecule, while 30-kDa antigen gave one major band at 30-kDa molecule and faint additional band at 32-kDa. The pI of 30kDa-and 32-kDa antigens were 4.3 and 4.6, respectively. The partial identity between these two antigens was observed through the same pattern of reactivity of antigens in the ELISA and precipitation pattern of immunodiffusion. We also examined the immunological activities of both purified antigens by splenocyte proliferation of BCG-infected mice. Proliferative response to the 30-kDa and 32-kDa antigens did not show significant difference. However, macrophage-like cells existed in spleen cells from BCG-infected mice were involved in the suppression of the proliferative response to mycobacterial antigen. These results suggest that the 30-kDa and 32-kDa antigens could be effectively purified by the IEF and reactions of partial identity between the two antigens were found. However, 30-kDa antigen was the more immunogenic antigen than 32-kilodalton.

국내에서 생장한 Kenaf의 해부학적 특성

권영만,황원중,권성민,조준형,이명구,김남훈 江原大學校 森林科學硏究所 2005 Journal of Forest Science Vol.21 No.-

국내에서 재배한 kenaf의 해부학적 특성을 광학현미경법으로 조사하였다. Kenaf의 수피부에는 인피섬유, 사부방사조직, 피층유세포 등이 존해하였고, 목질부는 도관, 목섬유, 방사조직 등으로 구성되어 있었다. 도관은 고립관공과 2~3개가 방사방향으로 복합한 복합관공이 많이 존재하였고, 방사조직은 평복, 직립, 방형세포가 모두 분포하고 있었다. 접선단면에서 방사조직은 단열 및 다열방사조직으로 구성되었다. 수피비의 인피섬유층은 생장기간과 함께 증가하였다. Anatomical properties of Kenaf cultivated in Korea was investigated using light microscopy. Bast fiber, phloem ray and cortex parencyma cell were observed in bast, and vessel, wood fiber and rat in core. A lot of solitary and multiple radial pores in core existed. The cell type of ray parenchyma in radial section was procumbent, upright and square sell. Uniseriate and multiseriate rays existed in tangential section. The layer of bast fiber in bast increased with in creasing the growth period.

Genetic heterogeneity of liver cancer stem cells

Minjeong Kim(Minjeong Kim),Kwang-Woo Jo(Kwang-Woo Jo),Hyojin Kim(Hyojin Kim),Myoung-Eun Han(Myoung-Eun Han),Sae-Ock Oh(Sae-Ock Oh) 대한해부학회 2023 Anatomy & Cell Biology Vol.56 No.1

Cancer cell heterogeneity is a serious problem in the control of tumor progression because it can cause chemoresistance and metastasis. Heterogeneity can be generated by various mechanisms, including genetic evolution of cancer cells, cancer stem cells (CSCs), and niche heterogeneity. Because the genetic heterogeneity of CSCs has been poorly characterized, the genetic mutation status of CSCs was examined using Exome-Seq and RNA-Seq data of liver cancer. Here we show that different surface markers for liver cancer stem cells (LCSCs) showed a unique propensity for genetic mutations. Cluster of differentiation 133 (CD133)-positive cells showed frequent mutations in the IRF2, BAP1, and ERBB3 genes. However, leucine-rich repeat-containing G protein-coupled receptor 5-positive cells showed frequent mutations in the CTNNB1, RELN, and ROBO1 genes. In addition, some genetic mutations were frequently observed irrespective of the surface markers for LCSCs. BAP1 mutations was frequently observed in CD133-, CD24-, CD13-, CD90-, epithelial cell adhesion molecule-, or keratin 19-positive LCSCs. ASXL2, ERBB3, IRF2, TLX3, CPS1, and NFATC2 mutations were observed in more than three types of LCSCs, suggesting that common mechanisms for the development of these LCSCs. The present study provides genetic heterogeneity depending on the surface markers for LCSCs. The genetic heterogeneity of LCSCs should be considered in the development of LCSC-targeting therapeutics.