Study on Recognition Method of Adhering Bars Based on Support Vector Machine

Guohua Liu,Liangyu Li,Bingle Liu 보안공학연구지원센터 2015 International Journal of Signal Processing, Image Vol.8 No.9

It is difficult to track, count and separate the moving bars at a high speed on production line for their overlap and accumulation. Therefore, it is necessary to establish a reliable, practical recognition and segmentation mechanism for the adhered bars. A new solution to the problem of bars adhesion is proposed: a support vector machine is constructed to recognize the adhesion type of bars by the feature vectors of training samples. The geometric feature values and moment feature values based on Blob regions in images are extracted, which is the input feature vector of support vector machine. The trained classifier is used for identifying the adhesion type of bars in images. Finally, classification and recognition is carried by support vector machine. The experimental results show that the recognition accuracy based RBF kernel achieves 100%. The method is feasible and effective for the recognition and segmentation of the adhered bars.

Investigate the Effect of Potassium on Nodule Symbiosis and Uncover an HAK/KUP/KT Member, GmHAK5, Strongly Responsive to Root Nodulation in Soybean

Liu Jianjian,Liu Jinhui,Cui Miaomiao,Chen Xiao,Liu Junli,Chen Jiadong,Chen Aiqun,Xu Guohua 한국식물학회 2022 Journal of Plant Biology Vol.65 No.6

Leguminous plants form root nodule organs with soil rhizobia bacteria, which can fix atmospheric nitrogen (N2) and supply ammonia to the host plant. It is established that the symbiotic N fixation efficiency is substantially influenced by plant nutrients, such as molybdenum (Mo), phosphorus (P), copper (Cu), and zinc (Zn). Potassium (K+) is the most abundant cation in plant cells; however, little evidence regarding the potential link between K nutrition and symbiotic N fixation efficiency is available to date. Here, we showed that K+ deficiency reduced the efficiency of symbiotic nitrogen fixation, and inoculated with rhizobia strain USDA110 could improve plant K+ acquisition. Furthermore, we identified a potassium transporter gene (GmHAK5) that was highly expressed in the root steles and in the vasculature cells of nodules. The GmHAK5 protein was localized at the plasma membrane and could rescue the growth phenotype of yeast K+ uptake-defective mutant. The results obtained from this study provides new insights for the understanding of the potential role of K+ nutrition in modulating symbiotic N fixation in soybean.

ATP6V0d2 Suppresses Alveoli Macrophage Alternative Polarization and Allergic Asthma via Degradation of PU.1

Liu Na,Feng Yuchen,Liu Huicheng,Wu Wenliang,Liang Yuxia,Li Pingfei,Wei Zhengping,Wu Min,Tang Zhao-Hui,Han Junyan,Cheng Xiang,Liu Zheng,Laurence Arian,Li Huabin,Zhen Guohua,Yang Xiang-Ping 대한천식알레르기학회 2021 Allergy, Asthma & Immunology Research Vol.13 No.3

Purpose Macrophages are important regulators of environmental allergen-induced airway inflammation and asthma. ATP6V0d2 is a subunit of vacuolar ATPase highly expressed in macrophages. However, the functions of ATP6V0d2 in the regulation of pathogenesis of allergic asthma remain unclear. The aim of this study is to determine the function and related molecular mechanisms of macrophage protein ATP6V0d2 in allergic asthma. Methods We compared the disease severity between female C57BL/6 wild-type and ATP6V0d2−/− mice in an ovalbumin (OVA)-induced asthma model. We also investigated the association of expression of ATP6V0d2, PU.1 and CCL17 with disease severity among asthmatic patients. Results The expression of ATP6V0d2 in sputum cells of asthmatic patients and in the lungs of OVA-challenged mice was enhanced compared to healthy subjects and their counterparts, respectively. However, ATP6V0d2-deficient mice exaggerated inflammatory cell infiltration as well as enhanced alternative activated macrophage (AAM) polarization and mucus production in an OVA-induced asthma model. Furthermore, we found that Atp6v0d2 promoted lysosomal degradation of Pu.1, which induced AAM polarization and Ccl17 production. Among asthma patients, ATP6V0d2 expression was inversely associated with disease severity, whereas PU.1 and CCL17 expression was positively associated with disease severity. Conclusions Our results identify macrophage Atp6v0d2, as an induced feedback inhibitor of asthma disease severity by promoting Pu.1 lysosomal degradation, which may in turn leads to reduced AAM polarization and Ccl17 production.

Numeral Description of Grain Size Effects of Tin Oxide Gas-Sensitive Elements and Evaluation of Depletion Layer Width

Jianqiao Liu,Guohua Jin,Zhaoxia Zhai,Faheema Fairuj Monica,Xuesong Liu 대한금속·재료학회 2015 ELECTRONIC MATERIALS LETTERS Vol.11 No.3

The grain size effects on tin oxide gas-sensitive elements are numerically described by the model of gradient-distributed oxygen vacancies, which extends the receptor function of semiconductors to the condition of inhomogeneous donor density in grains. The sensor resistance and the response to the reducing gas are formulated as functions of the grain size and the depletion layer width. The simulations show good agreement with the experimental results. The depletion layer width is estimated as 4 nm for the undoped SnO2 element, whereas the values are 2 and 7 nm for Sb-doped and Al-doped samples, respectively. The results are experimentally verified by the donor-doped SnO2 thin films, the depletion layer widths of which are evaluated on the basis of the correlation between the electrical resistance and the Sb-doping amount. The location of the Fermi level is found to be a crucial factor that dominates the evaluation results.

Simulation of the Grain Size Effect in Gas-Sensitive SnO2 Thin Films Using the Oxygen Vacancy Gradient Distribution Model

Jianqiao Liu,Zhaoxia Zhai,Guohua Jin,Yuan Li,Faheema Fairuj Monica,Xuesong Liu 대한금속·재료학회 2015 ELECTRONIC MATERIALS LETTERS Vol.11 No.1

The model of gradient-distributed oxygen vacancies is utilized in simulatingthe grain size effects of gas-sensitive SnO2 thin films. The distribution profileof oxygen vacancies has a grain size effect and the profile gradient correlatespositively with the radius of the grains. The simulation results show that thegrain size is a fundamental factor dominating the gas-sensing properties ofthin films. The potential barrier height and resistivity have significant grainsize effects when m is between 0.1 and 0.5 nm−1. The size effects on sensorresponse to stimulant gases can be enhanced by increasing the value of m orthe absolute value of α. Two expressions are used to simulate the grain sizeeffect of the sensor response. The expressions act similarly when α < 0.2. The simplified response provides a neat function to quantitatively explain thesensor performance on gases with low partial pressure. Although the accurate response is complicated, it is applicable to theentire concentration range. A small power-law exponent n is calculated from the accurate response expression when a high gasconcentration is employed, illustrating a “saturation effect” of the response.

Identification of Novel Subregions of LOH in Gastric Cancer and Analysis of the HIC1 and TOB1 Tumor Suppressor Genes in These Subregions

Jingcui Yu,Songbin Fu,Peng Liu,Xiaobo Cui,Yu Sui,Guohua Ji,Rongwei Guan,Donglin Sun,Wei Ji,Fangli Liu,An Liu,Yuzhen Zhao,Yang Yu,Yan Jin,Jing Bai,Jingshu Geng,Yingwei Xue,Jiping Qi,Ki-Young Lee 한국분자세포생물학회 2011 Molecules and cells Vol.32 No.1

Previously, we identified 3 overlapping regions showing loss of heterozygosity (LOH, R_1-R_3 from 11 to 30 cM) on chromosome 17 in 45 primary gastric cancers (GCs). The data indicated the presence of tumor suppressor genes (TSGs) on chromosome 17 involved in GC. Among the putative TSGs in these regions, HIC1 (in SR_1) and TOB1 (in SR_3) remain to be examined in GC. By immunohistochemistry (IHC), methylation-specific PCR (MSP) and western blot, we evaluated the expression and regulation status for HIC1 and TOB1 protein in GC. We narrowed down the deletion intervals on chromosome 17 and defined five smaller LOH subregions, SR_1-SR_5 (0.54 to 3.42 cM), in GC. We found that HIC1 had downregulated expression in 86% (91/106) and was methylated in 87% (26/30) of primary GCs. Of the primary GCs showing downregulation of HIC1 protein, 75% (18/24) had methylated HIC1 gene. TOB1 was either absent or expressed at reduced levels in 75% (73/97) of the GC samples. In addition, a general reduction was found in total and the ratio of unphosphorylated to phosphorylated TOB1 protein levels in the differentiated GC cell lines. Further analysis revealed significant simultaneous downregulation of both HIC1 and TOB1 protein in GC tissue microarray samples (67%, 52/78) and in primary GCs (65%, 11/17). These results indicate that silencing of HIC1 and TOB1 expression is a common occurrence in GC and may contribute to the development and progression of the disease.

Research on Process Modeling of Information Dissemination Based on Social Network

Guohua Zhang,Feng Bao,Zhijun Liu 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.7

According to the retweeting mechanism based on user relations, we introduce the contacted state of an agent, and build a model of information propagation process in social networks. In the model, agents have two absorbing states, that is, the infected and refractory state. Experimental prove that small spreading rete can make information diffuse widely, and the degree based density of infected agents increases with the degree monotonously. The influence between information occurs only when different information originates in the same local neighborhood.

Mesenchymal stem cell-derived exosomes as a new therapeutic strategy for liver diseases

Guohua Lou,Zhi Chen,Min Zheng,Yanning Liu 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-

The administration of mesenchymal stem cells (MSCs) as a therapy for liver disease holds great promise. MSCs can differentiate into hepatocytes, reduce liver inflammation, promote hepatic regeneration and secrete protective cytokines. However, the risks of iatrogenic tumor formation, cellular rejection and infusional toxicity in MSC transplantation remain unresolved. Accumulating evidence now suggests that a novel cell-free therapy, MSC-secreted exosomes, might constitute a compelling alternative because of their advantages over the corresponding MSCs. They are smaller and less complex than their parent cells and, thus, easier to produce and store, they are devoid of viable cells, and they present no risk of tumor formation. Moreover, they are less immunogenic than their parent cells because of their lower content in membrane-bound proteins. This paper reviews the biogenesis of MSC exosomes and their physiological functions, and highlights the specific biochemical potential of MSC-derived exosomes in restoring tissue homeostasis. In addition, we summarize the recent advances in the role of exosomes in MSC therapy for various liver diseases, including liver fibrosis, acute liver injury and hepatocellular carcinoma. This paper also discusses the potential challenges and strategies in the use of exosome-based therapies for liver disease in the future.

Propagation Properties of Electromagnetic Multi-Gaussian Schell Model Beams Propagating Through Atmospheric Turbulence

Guohua Wu,He Zhou,Tonggang Zhao,Hongzhan Liu,Zhengda Li,Xin Zhao 한국물리학회 2014 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.64 No.6

A model for electromagnetic multi-Gaussian Schell-model beams is introduced. An analyticalexpression for the cross-spectral density matrix of electromagnetic multi-Gausssian Schell-model(EMGSM) beams propagating through non-Kolmogorov turbulence is derived based on the extendedHuygens-Fresnel principle. With help of this expression, the average intensity, degree of coherence(DOC) and degree of polarization (DOP) of EMGSM beams are investigated in detail. Our resultsshow that by suitable choices of the source parameters, the distance that preserve a flat profile canbe prolonged. The effects of M and exponent value on DOC and DOP of EMGSM beams arestudied in detail.

LINC01232 Promotes Gastric Cancer Proliferation through Interacting with EZH2 to Inhibit the Transcription of KLF2

( Jing Liu ),( Zhen Li ),( Guohua Yu ),( Ting Wang ),( Guimei Qu ),( Yunhui Wang ) 한국미생물 · 생명공학회 2021 Journal of microbiology and biotechnology Vol.31 No.10

To clarify the role of long intergenic nonprotein-coding RNA 1232 (LINC01232) in the progression of gastric cancer and the potential mechanism, we analyzed the expression of LINC01232 in TCGA database using the GEPIA online tool, and the LINC01232 level in gastric cancer cell lines was detected by quantitative real time-polymerase chain reaction (qRT-PCR) as well. Cell proliferation assay, colony formation assay, transwell assay and tumor formation experiment in nude mice were conducted to observe the biological behavior changes of gastric cancer cells through the influence of LINC01232 knockdown. LncATLAS database and subcellular isolation assay were used for subcellular distribution of LINC01232 in gastric cancer cells. The interaction among LINC01232, zeste homolog 2 (EZH2) and kruppel-like factor 2 (KLF2) was clarified by RNA-protein interaction prediction (RPISeq), RNA immunoprecipitation (RIP), qRT-PCR and chromatin immunoprecipitation (ChIP) assay. Rescue experiments were further conducted to elucidate the biological function of LINC01232/KLF2 axis in the progression of gastric cancer. LINC01232 was upregulated in stomach adenocarcinoma (STAD) tissues and gastric cancer lines. LINC01232 knockdown inhibited the proliferative capacities of gastric cancer cells in vitro, and impaired in vivo tumorigenicity. LINC01232 was mainly distributed in the cell nucleus where it epigenetically repressed KLF2 expression via binding to the enhancer of EZH2, which was capable of binding to promoter regions of KLF2 to induce histone H3 lysine 27 trimethylation (H3K27me3). LINC01232 exerts oncogenic activities in gastric cancer via inhibition of KLF2, and therefore, the knockdown of KLF2 could reverse the regulatory effect of LINC01232 in the proliferative ability of gastric cancer cells.