The Molecular Mechanism of Long Non-Coding RNA MALAT1-Mediated Regulation of Chondrocyte Pyroptosis in Ankylosing Spondylitis

Wei Chen,Feilong Wang,Jiangtao Wang1,Fuyu Chen,Ting Chen 한국분자세포생물학회 2022 Molecules and cells Vol.45 No.6

Long non-coding RNAs (lncRNAs) may be important regulators in the progression of ankylosing spondylitis (AS). The competing endogenous RNA (ceRNA) activity of lncRNAs plays crucial roles in osteogenesis. We identified the mechanism of the differentially expressed lncRNA MALAT1 in AS using bioinformatic analysis and its ceRNA mechanism. The interaction of MALAT1, microRNA-558, and GSDMD was identified using integrated bioinformatics analysis and validated. Loss- and gain-of-function assays evaluated their effects on the viability, apoptosis, pyroptosis and inflammation of chondrocytes in AS. We found elevated MALAT1 and GSDMD but reduced miR-558 in AS cartilage tissues and chondrocytes. MALAT1 contributed to the suppression of cell viability and facilitated apoptosis and pyroptosis in AS chondrocytes. GSDMD was a potential target gene of miR-558. Depletion of MALAT1 expression elevated miR-558 by inhibiting GSDMD to enhance cell viability and inhibit inflammation, apoptosis and pyroptosis of chondrocytes in AS. In summary, our key findings demonstrated that knockdown of MALAT1 served as a potential suppressor of AS by upregulating miR-558 via the downregulation of GSDMD expression.

Involvement of Alternative Oxidase in the Regulation of Sensitivity of Sclerotinia sclerotiorum to the Fungicides Azoxystrobin and Procymidone

Ting Xu,Ya-Ting Wang,Wu-Sheng Liang,Fei Yao,Yong-Hong Li,Dian-Rong Li,Hao Wang,Zheng-Yi Wang 한국미생물학회 2013 The journal of microbiology Vol.51 No.3

Sclerotinia sclerotiorum is a filamentous fungal pathogen that can infect many economically important crops and vegetables. Alternative oxidase is the terminal oxidase of the alternative respiratory pathway in fungal mitochondria. The function of alternative oxidase was investigated in the regulation of sensitivity of S. sclerotiorum to two commercial fungicides, azoxystrobin and procymidone which have different fungitoxic mechanisms. Two isolates of S. sclerotiorum were sensitive to both fungicides. Application of salicylhydroxamic acid, a specific inhibitor of alternative oxidase, significantly increased the values of effective concentration causing 50% mycelial growth inhibition (EC50) of azoxystrobin to both S. sclerotiorum isolates, whereas notably decreased the EC50 values of procymidone. In mycelial respiration assay azoxystrobin displayed immediate inhibitory effect on cytochrome pathway capacity, but had no immediate effect on alternative pathway capacity. In contrast, procymidone showed no immediate impact on capacities of both cytochrome and alternative pathways in the mycelia. However, alternative oxidase encoding gene (aox) transcript and protein levels, alternative respiration pathway capacity of the mycelia were obviously increased by pre-treatment for 24 h with both azoxystrobin and procymidone. These results indicate that alternative oxidase was involved in the regulation of sensitivity of S. sclerotiorum to the fungicides azoxystrobin and procymidone, and that both fungicides could affect aox gene expression and the alternative respiration pathway capacity development in mycelia of this fungal pathogen.

SIMPLE SYNTHESIS OF UNIFORM α-Fe2O3 MICROSPHERES WITH EXCELLENT PHOTOCATALYTIC PERFORMANCE USING POLY (N-VINYL-2-PYRROLIDONE)

TING-TING ZHANG,TING-TING GUO,ZHI-PING WU,SI-WEI YANG,ZHOU-FENG WANG,FANG LIAO 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2013 NANO Vol.8 No.5

In this paper, a new simple approach has been developed for the preparation of α-Fe2O3 microspheres by a facile hydrothermal method using PVP as a surfactant. Uniform α-Fe2O3 microspheres could be routinely synthesized through solvothermal approach by controlling the PVP/FeCl3⋅6H2O ratio. The as-obtained α-Fe2O3 microspheres exhibit high efficiency on the decolorization of RhB aqueous solution in the presence of H2O2 at room temperature.

Luteolin Induced-growth Inhibition and Apoptosis of Human Esophageal Squamous Carcinoma Cell Line Eca109 Cells in vitro

Wang, Ting-Ting,Wang, Shao-Kang,Huang, Gui-Ling,Sun, Gui-Ju Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.11

Luteolin is a plant flavonoid which exhibits anti-oxidative, anti-inflammatory and anti-tumor effects. However, the antiproliferative potential of luteolin is not fully understood. In this study, we investigated the effect of luteolin on cell cycling and apoptosis in human esophageal squamous carcinoma cell line Eca109 cells. MTT assays showed that luteolin had obvious cytotoxicity on Eca109 with an $IC_{50}$ of $70.7{\pm}1.72{\mu}M$ at 24h. Luteolin arrested cell cycle progression in the G0/G1 phase and prevented entry into S phase in a dose- and time-dependent manner. as assessed by FCM. Luteolin induced apoptosis of Eca109 cells was demonstrated by AO/EB staining assay and annexin V-FITC/PI staining. Moreover, luteolin downregulated the expression of cyclin D1, survivin and c-myc, and it also upregulated the expression of p53, in line with the fact that luteolin was able to inhibit Eca109 cell proliferation.

Factors Related to Treatment Refusal in Taiwanese Cancer Patients

Chiang, Ting-Yu,Wang, Chao-Hui,Lin, Yu-Fen,Chou, Shu-Lan,Wang, Ching-Ting,Juang, Hsiao-Ting,Lin, Yung-Chang,Lin, Mei-Hsiang Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.8

Background: Incidence and mortality rates for cancer have increased dramatically in the recent 30 years in Taiwan. However, not all patients receive treatment. Treatment refusal might impair patient survival and life quality. In order to improve this situation, we proposed this study to evaluate factors that are related to refusal of treatment in cancer patients via a cancer case manager system. Materials and Methods: This study analysed data from a case management system during the period from 2010 to 2012 at a medical center in Northern Taiwan. We enrolled a total of 14,974 patients who were diagnosed with cancer. Using the PRECEDE Model as a framework, we conducted logistic regression analysis to identify independent variables that are significantly associated with refusal of therapy in cancer patients. A multivariate logistic regression model was also applied to estimate adjusted the odds ratios (ORs) with 95% confidence intervals (95%CI). Results: A total of 253 patients (1.69%) refused treatment. The multivariate logistic regression result showed that the high risk factors for refusal of treatment in cancer patient included: concerns about adverse effects (p<0.001), poor performance(p<0.001), changes in medical condition (p<0.001), timing of case manager contact (p=.026), the methods by which case manager contact patients (p<0.001) and the frequency that case managers contact patients (${\geq}10times$) (p=0.016). Conclusions: Cancer patients who refuse treatment have poor survival. The present study provides evidence of factors that are related to refusal of therapy and might be helpful for further application and improvement of cancer care.

Intravenous Flurbiprofen Axetil Enhances Analgesic Effect of Opioids in Patients with Refractory Cancer Pain by Increasing Plasma β-Endorphin

Wu, Ting-Ting,Wang, Zhi-Gang,Ou, Wu-Ling,Wang, Jun,Yao, Guo-Qing,Yang, Bo,Rao, Zhi-Guo,Gao, Jian-Fei,Zhang, Bi-Cheng Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24

Background: The study aimed to investigate the analgesic effect of a combination of intravenous flurbiprofen axetil and opioids, and evaluate the relationship between refractory pain relief and plasma ${\beta}$-endorphin levels in cancer patients. Materials and Methods: A total of 120 cancer patients was randomly divided into two groups, 60 patients took orally morphine sulfate sustained-release tablets in group A, and another 60 patients receiving the combination treatment of intravenous flurbiprofen axetil and opioid drugs in group B. After 7 days, pain relief, quality of life improvement and side effects were evaluated. Furthermore, plasma ${\beta}$-endorphin levels were measured by radioimmunoassay. Results: With the combination treatment of intravenous intravenous flurbiprofen axetil and opioids, the total effective rate of pain relief rose to 91.4%, as compared to 82.1% when morphine sulfate sustained-release tablet was used alone. Compared with that of group A, the analgesic effect increased in group B (p=0.031). Moreover, satisfactory pain relief was associated with a significant increase in plasma ${\beta}$-endorphin levels. After the treatment, plasma ${\beta}$-endorphin level in group B was $62.4{\pm}13.5pg/ml$, which was higher than that in group A ($45.8{\pm}11.2pg/ml$) (p<0.05). Conclusions: Our results suggest the combination of intravenous flurbiprofen axetil and opioids can enhance the analgesic effect of opioid drugs by increasing plasma ${\beta}$-endorphin levels, which would offer a selected and reliable strategy for refractory cancer pain treatment.

영화 <체르노빌 다이어리> 중 체르노빌 공간의 권력구조 연구

왕팅팅(Ting-Ting Wang),이종한(Jong-Han Lee) 한국애니메이션학회 2021 애니메이션연구 Vol.17 No.3

과학기술의 급속한 발전으로 생명안전 문제가 빈번히 발생하는 현대, 특히 코로나19의 배경 아래에서 역사적 경험을 통해 재난의 재발 방지를 위해 최선을 다하는 것은 미룰 수 없는 임무이다. 체르노빌 원전폭발은 이미 역사로 남았지만 그것이 남긴 문제는 아직도 적절하게 해결되지 않았다. ‘체르노빌’이라는 이 금기된 공간을 어떻게 적절하게 처리할지는 현대사회의 문제 중 하나이다. 본문은 브래들리 파커(Bradley Parker) 감독의 <체르노빌 다이어리>(2012)를 사례로 현대에서 체르노빌의 공간적 표현을 고찰한다. 이 영화는 자극을 추구하는 여행객들이 금지구역인 체르노빌로 진입하면서 겪는 끔찍한 상황을 그렸으며, 영화에서 나타난 체르노빌은 실제 체르노빌의 공간 상태와 부합한다. 영화에서 묘사한 체르노빌은 무인구역, 소비구역, 정치권력 규제구역 세 공간이 혼합된 장소이다. 본 논문에서는 세 공간이 작동하는 메커니즘과 상호 관계를 탐구하여 에리히 프롬(Erich Fromm)이 말한 건전한 사람과 건전한 사회의 구축에 기여할 것이다. 체르노빌은 무인구역, 소비구역, 정치권력 규제구역 세 공간이 혼합되어 서로를 제약한다. 체르노빌이라는 이 공간에서 국가는 주도적 위치를 차지하며, 무인구역과 소비구역의 운영 배후에는 국가의 정치권력이 어느 정도 관여하고 있다. 원전폭발로 인해 체르노빌은 정치당국에 의해 ‘예외상태’로 운영되면서, 이 운영 체제 하에서 국가법, 인권, 도덕윤리 등 문명사회의 체제와 규범이 사라지게 되었고, 이 공간에 들어온 개인의 생명은 순식간에 조르조 아감벤(Giorgio Agamben)이 말한 ‘벌거벗은 생명’으로 전락해 타인에 의해 생존권을 박탈당했다. 따라서 ‘건전한 사회’를 만들어 ‘건전한 개인의 생명’을 살리는 길을 고민하는 것은 매우 절실하다. In the contemporary era when the rapid development of science and technology has caused many life safety issues, especially in the context of the new crown pneumonia (Coronavirus 19) epidemic, it is the unshirkable responsibility of contemporary intellectuals to learn from historical experience and make every effort to prevent the recurrence of disasters. Although the Chernobyl nuclear disaster has become history, the problems left over from history are far from being properly resolved. How to properly handle the taboo space of “Chernobyl” is one of the leftover problems of disasters facing contemporary society. This article takes Bradley Parker’s thriller “Chernobyl: A Record of Ghost Towns”(2012, Chernobyl Diaries) as an example to examine the contemporary spatial presentation of Chernobyl. The film tells the horror experience of a group of tourists seeking excitement after entering the exclusion zone Chernobyl. The Chernobyl presented in the film fits well with the contemporary real-life Chernobyl space. The Chernobyl constructed by the film is a mixed field of no man’s land, consumer space, and political power discipline space. The author tries to explore the mechanisms of their respective operations and the relationship between them. I believe that from Erich Fromm) The sound people and the construction of a sound society in the mouth contribute a little. Chernobyl is a mixed and mutually restricted field of no-man’s land, consumption area, and political power discipline area; State political power occupies a dominant position in the Chernobyl space. Behind the operation of no-man’s land and consumer zone is the default and permission of state political power to a certain extent.Due to the nuclear disaster, Chernobyl was operated in an “exceptional state” by the political management department. Under this operating mechanism, civilized social systems and norms such as national laws, human rights, morals and ethics have disappeared. Individual lives instantly become what Agamben calls “bare life”, and they can be deprived of the right to survive by anyone else. Therefore, we urgently need to think about the path of constructing a “sound society” and then cultivating a “sound individual life”.

A Novel All-trans Retinoid Acid Derivative N-(3-trifluoromethyl-phenyl)-Retinamide Inhibits Lung Adenocarcinoma A549 Cell Migration through Down-regulating Expression of Myosin Light Chain Kinase

Fan, Ting-Ting,Cheng, Ying,Wang, Yin-Feng,Gui, Shu-Yu,Chen, Fei-Hu,Zhou, Qing,Wang, Yuan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.18

Aim: To observe the effects of a novel all-trans retinoid acid (ATRA) derivative, N-(3-trifluoromethyl-phenyl)-retinamide (ATPR), on lung adenocarcinoma A549 cells and to explore the potential mechanism of ATPR inhibiting of A549 cell migration. Materials and Methods: The cytotoxicity of ATRA and ATPR on A549 cells was assessed using MTT assay. Wound healing assays were used to analyze the influences of ATRA, ATPR, ML-7 (a highly selective inhibitor of myosin light chain kinase (MLCK)), PMA (an activator of MAPKs) and PD98059 (a selective inhibitor of ERK1/2) on the migration of A549 cells. Expression of MLCK and phosphorylation of myosin light chain (MLC) were assessed by Western blotting. Results: ATRA and ATPR inhibited the proliferation of A549 cells in a dose- and time-dependent manner, and the effect of ATPR was much more remarkable compared with ATRA. Relative migration rate and migration distance of A549 cells both decreased significantly after treatment with ATPR or ML-7. The effect on cell migration of PD98059 combining ATPR treatment was more notable than that of ATPR alone. Moreover, compared with control groups, the expression levels of MLCK and phosphorylated MLC in A549 cells were both clearly reduced in ATRA and ATPR groups. Conclusions: ATPR could suppress the migration and invasion of A549 cells, and the mechanism might be concerned with down-regulating the expression of MLCK in the ERK-MAPK signaling pathway, pointing to therapeutic prospects in lung cancer.

The Impact of Teacher-Student Interaction on Learning Performance - Focusing on the Mediating Effect of Learning Engagement -

왕정정 ( Wang Ting-ting ),이염남 ( Li Yan-nan ) 국제뇌교육종합대학원 인성교육연구원 2023 인성교육연구 Vol.8 No.2

본 연구는 교사-학생 상호작용이 학습 성과에 미치는 영향을 탐구하며, 학습 몰입의 매개 역할이라는 점에 집중하였다. 연구방법은 중국과 한국의 312명 대학(원)생을 대상으로 한 설문 조사를 포함하며, 연구목적을 검증하기 위해 SPSS 23.0을 사용하여 분석하였다. 연구결과를 살펴보면 교사-학생 상호작용이 학습 성과와 학습 참여에 긍정적으로 영향을 미치며, 학습 참여도 학습 성과에 긍정적인 영향을 미친다는 것을 확인하였다. 또한, 본 연구는 학습 참여가 교사-학생 상호작용의 학습 성과에 대한 영향을 매개한다는 결과를 검증하였다는 점이다. 교육 과정의 이러한 중요한 요소를 연결함으로써, 본 연구는 다양한 교육 환경에서 교육자가 교육과 학습의 질 효과를 향상시키려는 데 유용한 통찰력을 제공할 것이다. 또한 본 연구는 학습자 참여와 참여 유도에 대한 교육자에게 중요한 지침을 제공하며, 교육 정책 결정자에게는 교육 환경을 개선하여 교사-학생 상호작용과 학습 참여를 촉진하는 방법에 대한 의견을 제시함으로써 교육의 품질 향상과 학생의 학업 성취도 향상에 기여할 것이라고 생각한다. This study investigates the impact of teacher-student interaction on learning performance, with a particular emphasis on the mediating role of learning engagement. The research methodology involved a survey of 312 students from both China and Korea, and to verify the research objectives, the data were analyzed using SPSS 23.0. The results of the study confirm that teacher-student interaction positively influences both learning performance and learning engagement, while learning engagement also has a positive impact on learning performance. Furthermore, the study validates that learning engagement mediates the effect of teacher-student interaction on learning performance. Additionally, this research offers essential guidance for educators looking to foster student engagement and participation, and it provides recommendations for education policymakers on ways to improve the educational environment, thereby promoting teacher-student interaction and learning engagement, ultimately contributing to the enhancement of education quality and students' academic achievements.

Intestinal Anti-Inflammatory Effects of Selenized Ulva pertusa Polysaccharides in a Dextran Sulfate Sodium-Induced Inflammatory Bowel Disease Model

Yifan Wang,Han Ye,Leke Qiao,Chunying Du,Zhengpeng Wei,Ting Wang,Jingfeng Wang,Ruizhi Liu,Peng Wang 한국식품영양과학회 2021 Journal of medicinal food Vol.24 No.3

The purpose of this study was to examine the alleviative effects of selenized polysaccharides from Ulva pertusa (ulvan-Se) on inflammatory bowel disease (IBD) in mice. The dextran sulfate sodium (DSS)-induced IBD mouse model was used to explore the protective effects of ulvan-Se on the intestinal mechanical and immune barrier. At doses less than 1208 mg/kg·bw ulvan-Se showed no significant damage to Institute of Cancer Research (ICR) mice in an acute toxicity test. The results showed that DSS destroyed the mechanical barrier, which includes epithelial cells, while ulvan-Se promoted mRNA expression of tight junction proteins (zonula occludens protein 1, occludin, and claudin-1) and inhibited the infiltration of white blood cells into the intestines. At 100 mg/kg·bw, ulvan-Se enhanced the antioxidant capacity of mice more effectively than the 50 mg/kg·bw ulvan-Se. Furthermore, ulvan-Se improved the intestinal immune barrier by increasing immunoglobulin A and immunoglobulin M, while regulating the levels of interleukin (IL)-1β, interferon-γ, and IL-4. Oral administration of ulvan-Se also suppressed tumor necrosis factor-α, IL-1β, IL-6, and cyclooxygenase-2 mRNA expression mediated by the nuclear factor kappa B pathway. Taken together, our findings reveal that ulvan-Se could be used as a potential alternative supplement for reducing intestinal inflammation in IBD.