Preparation of Polyvinylpyrrolidone-Stabilized Fe0 Nanoparticles for Remediation of Cr(vi)-Spiked Soil

Lijun Yan,Chen He,Xuefeng Hu,Kokyo Oh 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6

A Game Theoretical Meditation on the Strategy of Industrial Competition

Lijun Chai,Zhihan Yan KINFORMS 2010 Management Review Vol.5 No.1

First, we analyze the present situation of market structure, enterprise behavior and organization performance in Chinese industry. Second, we integrate the model of competition and the strategy of technological innovation; third, we propose a game theoretic approach in studying strategy of industrial organization; finally the theoretical meaning and realism meaning of this study are given.

Lipid Emulsion as a Drug Delivery System for Breviscapine: Formulation Development and Optimization

Lijun Wei,Qizhe Quan,Gao Li,Yi-Dong Yan,Roshan Pradhan,김종오 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.6

In this study, we developed an optimized formulation of a breviscapine lipid emulsion (BLE) and evaluated the physicochemical properties and in vivo pharmacokinetics of BLE in rats. For the preparation of the lipid emulsion, soybean oil and oleic acid were used as the oil phase, lecithin and poloxamer 188 as surfactants and glycerol as co-surfactant. An optimized formulation consisting of soybean oil (10.0%), oleic acid (0.9%), lecithin (1.5%), poloxamer 188 (0.4%), and glycerol (2.25%) was selected. The results showed that the average particle size, polydispersity index, and zeta potential of the optimized formulation were 183.5 ± 5.5 nm, 0.098 ± 0.046, and −35.0 ± 2.5 mV, respectively. The BLE was stable for at least three month at room temperature. After a single intravenous dose of 4 mg/kg to rats, the AUC of scutellarin from the lipid emulsion was about 1.5-fold higher than that of the commercial product (breviscapine injection). In conclusion, the optimized formulation of BLE showed positive results over the commercial product in terms of the physicochemical properties and pharmacokinetics of BLE in rats.

Lipid Emulsion as a Drug Delivery System for Breviscapine: Formulation Development and Optimization

Wei, Lijun,Li, Gao,Yan, Yi-Dong,Pradhan, Roshan,Kim, Jong Oh,Quan, Qizhe 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.6

In this study, we developed an optimized formulation of a breviscapine lipid emulsion (BLE) and evaluated the physicochemical properties and in vivo pharmacokinetics of BLE in rats. For the preparation of the lipid emulsion, soybean oil and oleic acid were used as the oil phase, lecithin and poloxamer 188 as surfactants and glycerol as co-surfactant. An optimized formulation consisting of soybean oil (10.0%), oleic acid (0.9%), lecithin (1.5%), poloxamer 188 (0.4%), and glycerol (2.25%) was selected. The results showed that the average particle size, polydispersity index, and zeta potential of the optimized formulation were $183.5{\pm}5.5$ nm, $0.098{\pm}0.046$, and $-35.0{\pm}2.5$ mV, respectively. The BLE was stable for at least three month at room temperature. After a single intravenous dose of 4 mg/kg to rats, the AUC of scutellarin from the lipid emulsion was about 1.5-fold higher than that of the commercial product (breviscapine injection). In conclusion, the optimized formulation of BLE showed positive results over the commercial product in terms of the physicochemical properties and pharmacokinetics of BLE in rats.

<i>Arabidopsis</i> glucan synthase-like 10 functions in male gametogenesis

Huang, Lijun,Chen, Xiong-Yan,Rim, Yeonggil,Han, Xiao,Cho, Won Kyong,Kim, Seon-Won,Kim, Jae-Yean Elsevier 2009 Journal of plant physiology Vol.166 No.4

<P><B>Summary</B></P><P>Callose or <I>β</I>-1,3-glucan performs multiple functions during male and female gametophyte development. Callose is synthesized by 12 members of the glucan synthase-like (GSL) gene family in <I>Arabidopsis thaliana</I>. To elucidate the biological roles of <I>Arabidopsis</I> GSL family members during sexual development, we initiated a reverse genetic approach with T-DNA insertional mutagenesis lines. We screened T-DNA insertion lines for all members of the GSL gene family and detected homozygous mutant seedlings for all members except <I>GSL10</I>. Three independent alleles in <I>GSL10</I>, <I>gsl10-1</I>, <I>gsl10-3</I> and <I>gsl10-4</I> showed distorted segregation (1:1:0) of T-DNA inserts rather than Mendelian segregation (1:2:1). By genetic analysis through reciprocal cross, we determined that <I>gsl10</I> pollen could not be transmitted to descendent. The mutant pollen of <I>GSL10</I>/<I>gsl10</I> plants at tetrad and microspore stages were not different from that of wild type, suggesting that <I>GSL10</I> is not essential for normal microspore growth. Analysis of <I>GSL10</I>/<I>gsl10</I> hemizygous pollen during development revealed abnormal function in asymmetric microspore division. <I>gsl10</I> mutant microspores failed to enter into mitosis. Unlike the previously described functions of <I>GSL1</I>, <I>GSL2</I> and <I>GSL5</I>, <I>GSL10</I> involves an independent process of pollen development at the mitotic division stage.</P>

Identifying long non-coding RNAs and characterizing their functional roles in swine mammary gland from colostrogenesis to lactogenesis

Shi Lijun,Zhang Longchao,Wang Ligang,Liu Xin,Gao Hongmei,Hou Xinhua,Zhao Fuping,Yan Hua,Cai Wentao,Wang Lixian 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.6

Objective: This study was conducted to identify the functional long non-coding RNAs (lncRNAs) for swine lactation by RNA-seq data of mammary gland. Methods: According to the RNA-seq data of swine mammary gland, we screened lncRNAs, performed differential expression analysis, and confirmed the functional lncRNAs for swine lactation by validation of genome wide association study (GWAS) signals, functional annotation and weighted gene co-expression network analysis (WGCNA). Results: We totally identified 286 differentially expressed (DE) lncRNAs in mammary gland at different stages from 14 days prior to (-) parturition to day 1 after (+) parturition, and the expressions of most of lncRNAs were strongly changed from day –2 to day +1. Further, the GWAS signals of sow milk ability trait were significantly enriched in DE lncRNAs. Functional annotation revealed that these DE lncRNAs were mainly involved in mammary gland and lactation developing, milk composition metabolism and colostrum function. By performing weighted WGCNA, we identified 7 out of 12 lncRNA-mRNA modules that were highly associated with the mammary gland at day –14, day –2, and day +1, in which, 35 lncRNAs and 319 mRNAs were involved. Conclusion: This study suggested that 18 lncRNAs and their 20 target genes were promising candidates for swine parturition and colostrum occurrence processes. Our research provided new insights into lncRNA profiles and their regulating mechanisms from colostrogenesis to lactogenesis in swine. Objective: This study was conducted to identify the functional long non-coding RNAs (lncRNAs) for swine lactation by RNA-seq data of mammary gland.Methods: According to the RNA-seq data of swine mammary gland, we screened lncRNAs, performed differential expression analysis, and confirmed the functional lncRNAs for swine lactation by validation of genome wide association study (GWAS) signals, functional annotation and weighted gene co-expression network analysis (WGCNA).Results: We totally identified 286 differentially expressed (DE) lncRNAs in mammary gland at different stages from 14 days prior to (-) parturition to day 1 after (+) parturition, and the expressions of most of lncRNAs were strongly changed from day –2 to day +1. Further, the GWAS signals of sow milk ability trait were significantly enriched in DE lncRNAs. Functional annotation revealed that these DE lncRNAs were mainly involved in mammary gland and lactation developing, milk composition metabolism and colostrum function. By performing weighted WGCNA, we identified 7 out of 12 lncRNA-mRNA modules that were highly associated with the mammary gland at day –14, day –2, and day +1, in which, 35 lncRNAs and 319 mRNAs were involved.Conclusion: This study suggested that 18 lncRNAs and their 20 target genes were promising candidates for swine parturition and colostrum occurrence processes. Our research provided new insights into lncRNA profiles and their regulating mechanisms from colostrogenesis to lactogenesis in swine.

SOC balance‑based decentralized control strategy for hybrid energy storage in integrated power systems

Xueping Gao,Lijun Fu,Yan Zhang,Feng Ji 전력전자학회 2022 JOURNAL OF POWER ELECTRONICS Vol.22 No.12

The hybrid energy storage systems (HESSs) in vessel integrated power systems can support pulse load and improve system stability. However, the unbalanced SOC of different energy storage devices can cause over-charge and over-discharge which damages the energy storage devices and affects the stable operation of the entire system, especially when there are multiple groups of HESSs. Therefore, a decentralized control strategy for the HESSs in integrated power systems (IPSs) based on extended droop control combined with SOC balance control is proposed in this paper. In the proposed strategy, SOC recovery control is introduced to the virtual capacitance droop coefficient of the supercapacitor, and SOC equalization control is introduced to the virtual resistance droop coefficient of the lithium battery to adjust the output characteristics of the HESS according to the SOC. Then the frequency response characteristics are analyzed and the stability of the system is calculated. Finally, the hardware in loop simulation is conducted to verify the effectiveness of the proposed strategy. Comparisons show that the proposed strategy can compensate for the pulse load according to the responding characteristics of the HESS and achieve SOC balance between different energy storage devices under various working conditions.

A Demand Side Management Strategy to Reduce the Impact of High Power Pulse Load Integration

Gao Xueping,Zhang Yan,Ji Feng,Fu Lijun 대한전기학회 2022 Journal of Electrical Engineering & Technology Vol.17 No.5

High pulsed power loads (HPPLs) are highly non-linear and can cause signifi cant stability and power quality issues in a microgrid. Developing alternative methods for controlling and analyzing the microgrid with multiple HPPLs will provide insight into tradeoff s that can be made during the design phase. In this paper, a demand side management strategy of HPPLs in a microgrid is formulated. Firstly, the HPPLs are divided into energy storage connected high power pulse loads (EHPPLs) and direct connected high power pulse loads (DHPPLs) according to the power use of the back-end loads, and the energy management models of both the EHPPLs and the DHPPLs are built. Then the demand side management strategy to minimize the impacts of running HPPLs is proposed. Finally, three typical cases are studied to verify the fl exibility and eff ectiveness of the proposed method. The simulation and experiment results indicate that the proposed strategy can eff ectively reduce the peak power demand of the HPPLs, thus reducing the impact on the microgrid

Direct protection of cultured neurons from ischemia-like injury by minocycline

Wendy C. Huang,Yanli Qiao,Lijun Xu,Rachid Kacimi,Xiaoyun Sun,Rona G. Giffard,Midori A. Yenari 대한해부학회 2010 Anatomy & Cell Biology Vol.43 No.4

Minocycline, a tetracycline antibiotic, is now known to protect cells via an anti-inflammatory mechanism. We further explored this effect using an in vitro model of ischemia-like injury to neurons. Coculturing neurons with microglia, the brain’s resident immune cell, modestly increased cell death due to oxygen and glucose deprivation (OGD), compared to neurons alone. Treatment of cocultures with minocycline decreased cell death to a level significantly lower than that of neurons alone. Treatment of cocultures with minocycline or inhibitors of various immune mediators, also led to decreased cell death. Importantly, treatment of neuron cultures without added microglia with these same inhibitors of tissue plasminogen activator, matrix metalloproteinases, TNF-alpha and inducible nitric oxide synthase as well as minocycline also led to decreased cell death. Thus, anti-inflammatory treatments appear to be directly protective of neurons from in vitro ischemia.

Antisense oligodeoxynucleotides against dynamin-related protein 1 reduce remifentanilinduced hyperalgesia by modulating spinal N-methyl-D-aspartate receptor expression in rats

Songyi Zhou,Yizhao Pan,Yan Zhang,Lijun Gu,Leikai Ma,Qingqing Xu,Weijian Wang,Jiehao Sun 대한통증학회 2023 The Korean Journal of Pain Vol.36 No.3

Background: Spinal N-methyl-D-aspartate (NMDA) receptor activation is attributed to remifentanil-induced hyperalgesia (RIH). However, the specific mechanism and subsequent treatment is still unknown. Previous studies have shown that the dynamin-related protein 1 (DRP1)-mitochondria-reactive oxygen species (ROS) pathway plays an important role in neuropathic pain. This study examined whether antisense oligodeoxynucleotides against DRP1 (AS-DRP1) could reverse RIH. Methods: The authors first measured changes in paw withdrawal mechanical threshold (PWMT) and paw withdrawal thermal latency (PWTL) at 24 hours before remifentanil infusion and 4, 8, 24, and 48 hours after infusion. The expression levels of DRP1 and NR2B were measured after behavioral testing using Western blotting. In addition, DRP1 expression was knocked down by intrathecal administration of AS-DRP1 to investigate the effects of DRP1 on RIH. The behavioral testing, the expression levels of spinal DRP1 and NR2B, and dorsal mitochondrial superoxide were measured. Changes in mitochondrial morphology were assessed using electron microscopy. Results: After remifentanil exposure, upregulation of spinal DRP1 and NR2B was observed along with a reduction in PWMT and PWTL. In addition, AS-DRP1 improved RIH-induced PWTL and PWMT (P < 0.001 and P < 0.001) and reduced remifentanil-mediated enhancement of spinal DRP1 and NR2B expression (P = 0.020 and P = 0.022). More importantly, AS-DRP1 reversed RIH-induced mitochondrial fission (P = 0.020) and mitochondrial superoxide upregulation (P = 0.031). Conclusions: These results indicate that AS-DRP1 could modulate NMDA receptor expression to prevent RIH through the DRP1-mitochondria-ROS pathway.