몇 가지 항균제가 시험관내에서 내독소와 TNF-α, IL-6 분비에 미치는 영향

최정현,문건웅,김명훈,이동건,박윤희,김상일,김태연,유진홍,김양리,신완식,강문원 대한화학요법학회 1997 대한화학요법학회지 Vol.15 No.2

To evaluate antibiotic-induced endotoxin release(AIER) and its correlation with some cytokines, we measured endotoxin level and tumor necrosis factor alpha(TNF-α) and interleukin6(IL-6) production in mononuclear cells in vitro after exposure of Pseudomonas aeruginosa to antibiotics belonging to different class with two extreme concentrations. The tested concetration of antibiotics were set up according to peak serum level. The low concetration of ceftazidirne and low concentration of imiperiem increased AIER, but high concentration of ceftazideme, high concentration of ciprofloxacin, high concentration of cefoperazone/sulbactam, high concentration of amikacin, and high concentration of meropenem reduced AIER.Interestingly, combined treatment of these antibiotics markedly reduced AIER, But the major cyotkines, TNF-α and IL-6 were not affect by type and concettration of antibiotics, combined treatment of antibiotics, and level of endotoxin released by antiboitics. In this study, we observed AIER was different according to type of antibiotics, concentration of antibiotics, and combination of antibiotics, But AIER had poor correlation with TNF-α and IL-6 in Pseudomonas aeruginosa. It suggests that cytokine release is not solely dependent to endotoxin, but more complex cascade is needed. More invesfigations, such as endotoxin induced cytokine mRNA expression, relationship with penicillin-binding proteins and endotoxin-neutralizing effect of antibiotic itself, must be performed.

The Free Radical Scavenger NecroX-7 Attenuates Acute Graft-versus-Host Disease via Reciprocal Regulation of Th1/Regulatory T Cells and Inhibition of HMGB1 Release

Im, Keon-Il,Kim, Nayoun,Lim, Jung-Yeon,Nam, Young-Sun,Lee, Eun-Sol,Kim, Eun-Jung,Kim, Hyoung Jin,Kim, Soon Ha,Cho, Seok-Goo The American Association of Immunologists, Inc. 2015 JOURNAL OF IMMUNOLOGY Vol.194 No.11

<P>Graft-versus-host disease (GVHD) is a major complication associated with allogeneic hematopoietic stem cell transplantation. Despite the prominent role of the adaptive immune system, the importance of controlling the innate immune system in the pathogenesis of GVHD has recently been rediscovered. High-mobility group box 1 (HMGB1) is a crucial damage-associated molecular pattern signal that functions as a potent innate immune mediator in GVHD. In the present study, we investigated treatment of experimental GVHD through HMGB1 blockade using the compound cyclopentylamino carboxymethylthiazolylindole (NecroX)-7. Treated animals significantly attenuated GVHD-related mortality and inhibited severe tissue damage. These protective effects correlated with the decrease in HMGB1 expression and lower levels of reactive oxidative stress. Additionally, NecroX-7 inhibited the HMGB1-induced release of TNF and IL-6, as well as the expression of TLR-4 and receptor for advanced glycation end products. We also observed increased regulatory T cell numbers, which may be associated with regulation of differentiation signals independent of HMGB1. Taken together, these data indicate that NecroX-7 protects mice against lethal GVHD by reciprocal regulation of regulatory T/Th1 cells, attenuating systemic HMGB1 accumulation and inhibiting HMGB1-mediated inflammatory response. Our results indicate the possibility of a new use for a clinical drug that is effective for the treatment of GVHD.</P>

디지털 영상을 이용한 수복재의 변색에 관한 연구

이용근,이건일,임미경 대한치과기재학회 1996 대한치과재료학회지 Vol.23 No.2

The purpose of this study was to find correlations among the color differences measured by three methods. Those were 1) spectrophotometric method(Differential Colorimeter, Model TC-6FX, Denshoku, Japan), 2) digital image method-photographed by digital camera(KODAK Digital Camera 40, Kodak, U.S.A.) and processed by computer program(Adobe Photoshop 3.0), 3) 35 mm standard slide method-took photographic color slide, read using scanner(Nikon 35 mm Film scanner, LS-3510 AF, Nikon, Japan) and processed by computer program(Adobe Photoshop 3.0). The color of resin modified glass ionomers were determined in vitro before and after thermocycling for 500 to 2,000 cycles. The values of CIELAB ΔE*, L*, a*, b*, r, g, b, r*, g*,b* were analyzed to find correlations among them. From the experiment, the following results were obtained: 1. There were no statistically significant correlation among the values of CIELAB ΔE*, ΔC measured by digital camera and ΔC* measured by color slide and scanner (Sig. F>0.05). 2. There was a statistically significant correlation between the values of CIELAB L* and GR measured by digital camera(Sig. F=0), and some pairs showed significant correlation among CIELAB a*, b* value and r, g, b value determined by digital camera and r*, g*, b* value determined by color slide and scanner. 3. The values of r, g, b determined by digital camera, and the values of r*, g*, b* value determined by color slide and scanner showed significant correlation between each corresponding values (Sig. F=0). In this case, correlation coeffcient were 0.339-0.413. 4. In the same materials, the degree of color was different depending on the shades, and of some shades showed of materials clearly different color change depending the measuring method.

수복재의 적정 방사선 불투과도에 관한 연구

이용근,임미경,문상은,이건일 大韓齒科器材學會 1995 대한치과재료학회지 Vol.22 No.2

The purpose of this study was to investigate the level of the radiopacity of composite resins, glass ionomers for filling and cements, and to determine the optimum level of radiopacity that is the most appropriate for the radiologic diagnosis of secondary caries. The experiments were performed in two parts. In the first part, the radiopacities of specimens of seven composite resins, two glass ionomers and four cements were measured by densitometer and those of the combined specimens were also measured. In the second part, caries was simulated by grooves of the depth of 0.5, 1.0, 1.5, 2.0mm in aluminium blocks of 4mm. thick. Specimens of 2, 4, 6mm. thick were mounted on the caries simulation block, and the differences of radiopacities between caries portion and noncaries portion were analyzed. The following results were obtained : 1. The radiopacity of dental materials at 2mm thickness displayed a wide range of radiopacity, from 1.14 to 11.70mm. aluminium equivalents. 2. Most of the cements showed significantly higher radiopacity values of 8.78 to 11.70mm. aluminium equivalents, and those of ZPC were higher than any other materials used in this study. 3. Filling materials with radiopacity similar to enamel showed a tendency to have a sufficient degree of contrast facilitate the detection of underlying recurrent caries.

방사선 불투과성 치과용 아크릴릭 레진의 개발

이용근,이건일,이성재 대한치과기재학회 1997 대한치과재료학회지 Vol.24 No.2

Aspiration or ingestions of fragments of dental appliances are common occurrences. If they have proper radiopacity, their localization in and progress through the gastrointestinal tract or trachea can be more effective. To make radiopaque dental acrylic resin, many radiopaque materials, such as iodide compound, magnesium oxide, silver alloy, bismuth compound or barium compound were added into dental carylic resins. However the results were not so satisfactory as to be used clinically. The purpose of this study was to develop a radiopaque dental acrylic resin, which has clinically detectible radiopacity with minimal changes of mechanical and esthetic properties. The radiopacity, color change(CIE ΔE), transverse strength, modulus of rupture and Vickers hardness of acrylic resins were determined after mixing radiopaque glass(SiO₂ 50%, BaO 30%, B₂O₃ 10% and Al₂O₃ 10%) of 10∼50wt%, silane coated radiopaque glass of 10∼50 wt%, triphenyl bismuth of 5∼20% or titanium oxide of 10%. The specimens were thermocycled in 5 and 55℃ distilled water for 1,000 times, after then the radiopacity, color change and Vickers hardness were re-measured. The radiopacity of specimens on the X-ray films was measured with Photoshop program with a film scanner. The color change was determined with differential colorimeter(Model TC-6FX, Tokyo Denshoku, Japan). and the Vickers hardness was measure with microhardness tester(Mitsuzawa, Japan). The flexural strength and modulus of rupture were deterined using the Universal Testing Machine(Zwick 1456, Z020, Germany) with the cross head speed of 5mm/min. The following results were obtained : 1. After mixing silane coated radiopaque glass about 30 wt%, the radiopacity and modulus of rupture increased significant(p<0.05), however the flexural strength and Vickers hardness did not changed significantly(p>0.05) compared to unmodified dental acrylic resins. So, this compost to unmodified dental acrylic resins. So, this composition can be used as a successful radiopaque dental acrylic resin. 2. After mixing radiopaque glass, the flexural strength decreased significantly as the mixing ratio of glass increased(p<0.05). Also the Vickers hardness decreased after mixing in the case of heat cured duntal acrylic resins. 3. After mixing triphenyl bismuth, the flexural strength and Vickers hardness decreased significantly in the case of heat cured duntal acrylic resins(p<0.05). 4. After mixing titanium dioxide, the radiopacity increased significantly(p<0.05), and mechanical properties did not change significantly(p>0.05). However, translucency decreased definitely.

수동형 Stainless Steel, Nickel-Titanium 및 엔진 구동형 Nickel-Titanium File의 근관형성 능력에 관한 비교 연구

이황,임미경,이건일,이용근 大韓齒科保存學會 1998 Restorative Dentistry & Endodontics Vol.23 No.1

The aim of this study was to determine the shaping ability of stainless-steel K file (S-S K file), nickel-titanium K file (Ni-Ti K file) and engine driven nickel-titanium file (Quantec file) in resin simulated root canal. Computed tomography was used to evaluate the change of the root canal morphology. Thirty nine resin simulated root canal were divided into four groups (A:12, B:12, C:12, D:3). Resin simulated canals were scanned by computed tomography before instrumentation (1st C-T scan). Canals were instrumented using step back preparation technique with S-S K file in group A and Ni-Ti K file in group B. Group C was prepared with engine driven Ni-Ti file. Group D was uninstrumented to compare the 1st C-T scan images with 2nd C-T scan images of root canal. Instrumented canals were again scanned using computed tomography (2nd C-T scan), and reformated images of the uninstrumented canals were compared with images of the instrumented canals. In the sections of 2mm and 6mm from the apex, Quantec file caused significantly less canal transportation than S-S K file and Ni-Ti K file (p<0.05). Quantec file produced more centered than S-S- K file and Ni-Ti K file in the sections of 2mm and 4mm from the apex (p<0.05). There was no significant difference in the removed volume of canals among the each groups (p>0.05). However the removed canal volume from the apex to 5mm were significantly higher than them from 5mm to 1mm (p<0.05) in each groups. Under the conditions of this study, preparation with Quantec file was more effective and produce more appropriate canal shapes than S-S K file and Ni-Ti K file.

치근단낭과 육아종의 디지털방사선학적 비교연구

이건일,진연화 大韓口腔顎顔面 放射線學會 1999 Imaging Science in Dentistry Vol.29 No.1

The purpose of this study was to investigate whether a radiometric analysis of radicular cysts and periapical granulomas is useful in the differential diagnosis. In this experiment, twenty-nine periapical radiographs of the radicular cyst and those periapical granuloma were used. The periapical radiography was taken by intraoral paralleling device. The X-ray film was digitized and digitally filtered to reduce film-grain noise. We estimated density difference of the inner/outer area, roundness or circularity, bone profile or scan line of the margin and cumulative percentage frequency curve of radicular cyst & periapical granuloma. The obtained results were as follows; 1. The differences in density between ROIs of inner and outer area of radicular cysts were smaller than those of periapical granulomas. 2. The equivalent circular diameter was over 6.3mm, there was significant difference between periapical cyst and periapical granuloma. 3. In differential diagnosis of radicular cyst and periapical granuloma using bone profile, sensitivity, specificity and accuracy were considerable high(0.83, 0.86, 0.86) respectively. 4. Cumulative percentage frequency curve of the radicular cyst was closer to the pseudo-pixel value of 50 than average curve, whereas periapical granuloma was closer to that of 0. Hence we conclude that digital radiometric features might be useful in the differential diagnosis between radicular cyst and periapical granuloma.(J Korean Oral Maxillofac Radiol 1999:29:239-252)

흡입관에 부착된 이젝터내의 유동특성에 관한연구

이행남,박길문,모양우,이덕구,설재림,김건일 朝鮮大學校 機械技術硏究所 2004 機械技術硏究 Vol.7 No.2

The ejector is used to get low pressure, and it has been applied to a lot of industry field like the heat engine, the fluid instrument power plant, the food industry, environment industry etc. because there are not any problem even it is mixed with kinds of liquid, gas, and solid. The flow characteristics in diffuser are investigated by PIV and CFD. The agreement between numerical analysis and experiment shows the validity of this study and the results of this study would be useful to the engineers who design for the flow systems for heating, ventilation, air conditioning and wastewater purification plants.

수종의 암세포주와 섬유모세포주에서 taxol과 전리방사선이 세포독성과 prostaglandin생성에 미치는 영향

이건일,유동수 大韓口腔顎顔面 放射線學會 1998 Imaging Science in Dentistry Vol.28 No.1

The author evaluated the effects of taxol, a microtubular inhibitor, as a possible radiation sensitizer and the production of prostaglandins on three human cancer cell lines(KB, RPMI-2650 and SW-13)and one murine cell line(L929). Each cell line was divided into four groups(control, taxol only. radiation only and combination of taxol and radiation). The treatment consisted of a single irradiation of 10 Gy and graded doses(5, 50, 100, 200, 300, 500 nM) of taxol for a 24-h period. The cytotoxicity of taxol alone was measured at 1 day after(1-day group) and 4 days after (4-day group) the treatment. The survival ratio of cell was analyzed by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-dimethyl tetrazolium bromide) test, Prostaglandins(PGE2 and PGI2) were measured in the culture medium by a radioimmunoassay. The results obtained were as follows. 1. There was a significantly increased cytotoxicity of KB cells in 4-day group than those in 1-day group. There was a high correlation between doses of taxol and cell viability in both groups(1-day group R=0.82741, 4-day group R=0.84655). 2. There was a significantly increased cytotoxicity of RPMI-2650 cells treated with high concentration of taxol in 4-day group than those in 1-day group. Also there was a high correlation between doses of taxol and cell viability in 4-day group(R=0.93917). 3. There was a significantly increased cytotoxicity of SW-13 cells treated with high concentration of taxol in 4-day group than those in 1-day group. However no high correlation was observed between doses of taxol and cell viability in both groups(1-day group R=0.46362, 4-day group R=0.65425). 4. There was a significantly increased cytotoxicity of L929 cells treated with low concentration of taxol in 4-day group than those in 1-day group. At the same time, there was a low correlation between doses of taxol and cell viability in both groups(1-day group R=0.34237, 4-day group R=0.23381). 5. In 1-day group of L929 cells higher cytotoxicities were observed in the groups treated with 500 nM taxol than given 10 Gy radiation alone. L929 cells in 1-day group alone showed a radiosensitizing effect by taxol. 6. In addition to L929 cells, all cancer cells treated with a combination of taxol and radiation in 4-day group appeared to have some fragmented nuclei and to float on the medium. In addition, L929 cells appeared to be more confluent. 7. The level of PGE₂ production was the highest in the contol KB cells. This appeared to increase in every experimental group0 of all three cancer cells except L929 cells. There was a significantly increased production of PGE₂ in SW-13 cells treated with a combination taxol and radiation compared to the other experimental groups. 8. The level of PGI₂ production in the contol group of RPMI-2650 cells was the highest. This appeared to increase in every experimental group of all cells except in SW-13 cells. This also increased significantly in RPMI-2650 cells treated with a combination of taxol and radiation compared to the other experimental groups.

치과용 아크릴릭 레진의 방사선 불투과도에 관한 연구 : 황산바륨과 요오드 화합물 첨가

이건일,이용근,정성우 大韓口腔顎顔面 放射線學會 1996 Imaging Science in Dentistry Vol.26 No.2

Aspirating or swallowing foreign bodies is a common occurrence. If they are wholly or partly radiopaque, their localization in and progress through the gastrointestinal tract can be more effective. Of the dental origin foreign materials swallowed, the most common things are fragments of anterior maxillary partial denture. But the radiopacity of denture base resins is not sufficient to determine the location of the objects. The purpose of this study was to develop a radiopaque dental acrylic resin, which has clinically detectible radiopacity with minimal change of mechanical properties and color. The radiopacity, color change(CIE △E)and microhardness of acrylic resins were determined after mixing barium sulfate of iodide compound. Thermocycling course was conducted to determine the change of characteristic of resins after using for a long time in the mouth. Five or ten percent of barium sulfate to total weight of cured material was mixed with heat curing dental acrylic resin or chemically curing orthodontic resin. In the case of iodide compound, the mixing ratio was two or three percent. After mixing the high radiopaque materials. resin was cured to 20×20×2 mm plate, polished with #600 sand paper and finally polished with Microcloth(Buehler). The specimens were thermocycled in 5 and 55 ℃ distilled water for 2,000 times, and the measurement of radiopacity, color and Vickers hardness was repeated every 500 times thermocycling. The radiopacity of specimens on the X-ray films was measured with densitometer(X-rite). The color change was determined with differential colorimeter (Model TC-6FX, Tokyo Denshoku), and the Vickers hardness number was measured with microhardness tester(Mitsuzawa). The following results were obtained: 1. All the three variables, the kinds of acrylic resins, the mixing or the kinds of high radiopaque materials and thermocycling, had combined effect on the radiopacity of the dental acrylic resins(p<0.01). 2. The two variables, the mixing or the kinds of high radiopaque materials and thermocycling, influenced on the radiopacity of the dental acrylic resins(P<0.01). But the kinds of acrylic resins did not influence on the color change of mixed dental acrylic resins(p>0.05). 3. Each of the three variables, the kinds of acrylic resins, the mixing or the kinds of high radiopaque materials and thermocycling, influenced on the radiopacity of dental acrylic resins (P<0.01). 4. The high radiopaque materials used in this study did not yield clinically usable radiopacity, and the color change was great after mixing those materials.