홀스타인 젖소의 원유내 acetone과 β-hydroxybutyrate acid 함량에 영향을 미치는 환경요인

조광현,조충일,이준호,박경도,Cho, Kwang-Hyun,Cho, Chung-Il,Lee, Joon-Ho,Park, Kyung-Do 한국데이터정보과학회 2015 한국데이터정보과학회지 Vol.26 No.3

본 연구는 젖소의 검정기록, 378,086개를 이용하여 케토시스 진단 지시형질로 이용되는 유량내 아세톤과 ${\beta}$-히드록시부틸산의 함량에 영향을 미치는 환경요인을 분석하였다. 형질별 목장, 비유단계, 산차, 착유시간 및 월령에 대한 유의성 검정 결과 모든 요인들에서 고도의 유의성 (p < 0.01)이 나타났으며, 가장 큰 환경요인은 비유단계였다. 1회 착유량, 아세톤과 ${\beta}$-히드록시부틸산의 함량에 대한 월령의 1차 회귀계수는 모두 정 (+)의 관계, 2차 회귀계수는 부 (-)의 관계를 나타내었으며, 비유 2단계 (36~65일) 비유량의 최소자승평균은 19.06kg으로 비유말기에 비하여 6.51kg 높았다. 아세톤과 ${\beta}$-히드록시부틸산의 함량에 대한 최소자승평균은 비유 1단계 (5~35일)에서 각각 0.1929mM/L과 0.0742mM/L로 가장 높았으며, 착유일수에 따라 감소하다가 비유말기 소폭 증가하는 경향을 나타내었다. 1산에 대한 아세톤과 ${\beta}$-히드록시부틸산의 함량의 최소자승평균은 각각 0.1414mM/L과 0.0522mM/L로 2차산 이상의 평균보다 높았으며, 오후착유에 대한 아세톤과 ${\beta}$-히드록시부틸산의 함량의 최소자승평균이 각각 0.1372mM/L과 0.0534mM/L로 오전착유보다 일괄적으로 높게 나타났다. Using 378,086 lactation records on dairy cattle, environmental factors influencing acetone and ${\beta}$-hydroxybutyrate acid contents in raw milk which are used as ketosis diagnosis indicator traits were analyzed in this experiment. Significance testing was conducted on farm, lactation stage, parity, milking time and month of age by traits. The results of this experiment indicated that there was a highly significant (p < 0.01) difference in all factors and lactation stage was the most significant factor. Linear regression coefficients of month of age on daily milk yields and acetone and ${\beta}$-hydroxybutyrate acid contents were all positive, while their quadratic linear regression coefficients were negative. Least square means for milk yield at second lactation stage (36~65 days) was 19.06kg which was higher than that of late lactation stage by 6.51kg. Least square means for acetone and ${\beta}$-hydroxybutyrate acid contents at the first lactation stage (5~35 days) were highest (0.1929mM/L and 0.0742mM/L, respectively), and there was a trend that they decreased as the milking progressed, but increased slightly at the late stage of milking. However, least square means for acetone and ${\beta}$-hydroxybutyrate acid contents at the first parity were 0.1414mM/L and 0.0522mM/L, respectively, which were higher than the average milk yield after the second parity. Least square means for acetone and ${\beta}$-hydroxybutyrate acid contents of PM milk yield (0.1372mM/L and 0.0534mM/L, respectively) were higher than those of AM milk yield collectively.

$La^{3+}$에 의한 토마토 뿌리조직 마이크로솜 $H^+-ATPase$ 활성저해

조광현,김영기,Cho, Kwang-Hyun,Kim, Young-Kee 한국응용생명화학회 2003 한국농화학회지 Vol.46 No.2

$H^+-ATPase$ 활성을 조절할 수 있는 물질을 찾기 위하여 토마토 뿌리조직으로부터 마이크로솜을 분리하고 $La^{3+}$의 효과를 조사하였다. 원형질막 및 액포막에 위치하는 $H^+-ATPase$의 활성은 각각의 선택적 저해제인 vanadate와 $NO_3-$의 처리시 감소하여, $La^{3+}$이 원형질막 및 액포막 $H^+-ATPase$ 활성을 모두 저해함을 확인하였다. 원형질막과 액포막 $H^+-ATPase$ 활성을 50% 저해하는 $La^{3+}$ 농도인 Ki 값은 각각 57, $78\;{\mu}M$이었다. $La^{3+}$에 의한 저해효과는 Triton X-100을 처리한 leaky 마이크로솜에서도 얻어져, $La^{3+}$이 이온채널의 존재와 관계없이 $H^+-ATPase$의 활성을 직접적으로 저해함을 확인하였다. 한편, Lak의 활성저해 효과는 ATP 농도 증가로 감소하였고, ATP의 효과는 농도 의존적으로 나타났으며, 7 mM ATP 의해 $La^{3+}$에 의한 $H^+-ATPase$ 활성 저해가 완전히 억제되었다. 이러한 결과로부터 $La^{3+}$은 원형질막과 액포막의 $H^+-ATPase$들에 결합하여 ATP 결합친화력을 감소시킴으로써 활성을 저해하며, 뿌리조직 $H^+-ATPase$의 활성조절제로 이용이 가능함을 확인하였다. In order to find a chemical agent which is able to modulate the activity of $H^+-ATPase$, microsomal preparation was obtained from the root tissue of tomato plant and the effect of $La^{3+}$ was measured. The activities of plasma and vacuolar membrane $H^+-ATPase$ were analyzed by the inhibited activities using their specific inhibitors, vanadate and $NO_3-$, respectively. $La^{3+}$ inhibited microsomal ATPases in a dose-dependent manner and the inhibitory effect of $La^{3+}$ was suppressed by both vanadate and $NO_3-$, implying that $La^{3+}$ inhibits both plasma and vacuolar membrane $H^+-ATPase$. The Ki. values of $La^{3+}$which inhibit 50% of the activities of plasma and vacuolar membrane $H^+-ATPase$ were 57 and $78\;{\mu}M$, respectively. The $H^+-ATPase$ of the leaky microsomes made by the treatment of Triton X-100 were also inhibited by $La^{3+}$, suggesting that $La^{3+}$ directly inhibits both enzymes. Meanwhile, the inhibitory effect of $La^{3+}$ was decreased by increasing the concentration of ATP, The effect of ATP was also concentration-dependent and 7 mM ATP completely removed the inhibitory effect of $La^{3+}$. These results imply that $La^{3+}$ inhibits both plasma and vacuolar membrane $H^+-ATPases$ by decreasing the binding affinity of ATP and $La^{3+}$ can be used to control the activity or root $H^+-ATPases$.

얼굴에 발생한 Epstein-Barr Virus 감염과 연관된 수포성 발진

조광현 ( Kwang Hyun Cho ) 대한피부과학회 2018 대한피부과학회지 Vol.56 No.1

Epstein Barr virus (EBV)-associated vesicular eruptions on the face have been referred to as hydroa vacciniforme or hydroa vacciniforme-like eruptions. It is well recognized that these eruptions develop as a manifestation of T/natural killer-cell lymphoproliferative disorders associated with EBV infection. Although there is strong evidence for the existence of a pathogenic relationship between these eruptions and ultraviolet light exposure, every patient with these eruptions does not show photosensitivity. Over the last 20 years, the author has encountered approximately 30 patients presenting with EBV-associated vesicular eruptions of the face. This review describes the author’s clinical experience of managing EBV-associated vesicular eruptions of the face. (Korean J Dermatol 2018;56(1):1∼9)

BGP/IPsec VPN망에서 보안이 보장되는 LSP수립을 위한 RSVP 확장

조광현(Kwang-Hyun Cho),윤미라(Mi-Ra Yoon),김성운(Sung-Un Kim) 한국정보과학회 영남지부 2003 한국정보과학회 영남지부 학술발표논문집 Vol.11 No.1

피지선 모반의 표피에서 케라틴 및 Involucrin 발현

조광현(Kwang Hyun Cho),김영걸(Young Gull Kim),조미경(Mi Kyung Cho),지제근(Je Geun Chi) 대한피부과학회 1992 대한피부과학회지 Vol.30 No.3

Thirty two cases of nevus sebaceus were studied by immunohistochemical staining employing three anticytokeratin antibodies(34bE, 34bB, CAM 5.2) and anti-involucrin antibody in order to clarify the biochemical characteristics of the covering epidermis of nevus sebaceus. An attempt was made to compare the expression pattern of these proteins in the epidermis of nevus sebaceus with that in normal skin and in epidermal nevus. Serial sections in all cases were also stained with PAS in attempt to correlate these protein expression with the amount of glycogen in the epidermis of nevus sebaceus. The following results are obtained. 1. The expression of cytokeratin and involucrin in the epidermis of nevus sebaceus was changed as the age of the lesions increased, but we could not identify a uniform pattern according to the age of lesions. 2. Several CAM 5.2 positive cells were found in the basal layer of the epidermis of nevus sebaceus lesion obtained from patients over 10 years of age. 3. The expression of cytokeratin and involucrin did not show any correlation with glycogen content. These results suggest that the covering epidermis of the lesion of nevus sebaceus may not be nevoid proliferation of epidermal nevus. (Kor J Dermatol 1992; 30(3): 279-290)

주변조건부 변수를 이용한 의사결정나무모형 생성에 관한 연구

조광현,박희창,Cho, Kwang-Hyun,Park, Hee-Chang 한국데이터정보과학회 2012 한국데이터정보과학회지 Vol.23 No.2

데이터마이닝은 주어진 데이터베이스에서 항목간의 흥미로운 관계를 찾아내는 기법으로서 의사결정나무는 데이터마이닝의 대표적인 알고리즘이라고 할 수 있다. 의사결정나무는 관심대상이 되는 집단을 몇 개의 소집단으로 분류하거나 예측을 수행하는 방법이다. 일반적으로 연구자가 의사결정나무 모형을 생성 할 때 모형 생성의 기준 및 입력 변수의 수에 따라 복잡한 모형이 생성되기도 한다. 특히 의사결정나무 모형에서 입력 변수의 수가 많을 경우 생성된 모형은 복잡한 형태가 될 수 있고, 모형 분석이 어려울 수도 있다. 만일 입력변수에서 주변조건부 변수 (매개변수, 외적변수)가 존재한다면 이 입력변수는 직접적인 관련성이 없는 것으로 판단한다. 이에 본 논문에서는 주변조건부 변수를 고려하여 의사결정나무모형을 생성하는 방법을 제시하고 그 효율성을 파악하기 위하여 실제 자료에 적용하고자 한다. Data mining is a method of searching for an interesting relationship among items in a given database. The decision tree is a typical algorithm of data mining. The decision tree is the method that classifies or predicts a group as some subgroups. In general, when researchers create a decision tree model, the generated model can be complicated by the standard of model creation and the number of input variables. In particular, if the decision trees have a large number of input variables in a model, the generated models can be complex and difficult to analyze model. When creating the decision tree model, if there are marginally conditional variables (intervening variables, external variables) in the input variables, it is not directly relevant. In this study, we suggest the method of creating a decision tree using marginally conditional variables and apply to actual data to search for efficiency.

Chair`s Lecture : EBV associated hydroa vacciniforme-like eruption

조광현 ( Kwang Hyun Cho ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2

Hydroa vacciniforme (HV) is a rare self-limiting disease developing in childhood. It is characterized by vesicles and crust formation on the skin after sun exposure. Recently, cases with HV-like eruptions and severe systemic symptoms have been described in Asia and Latin America. It had been regarded as a different entity from typical HV. But latent Epstein-Barr virus (EBV) infection have also been detected in the patients with typical HV and the possibility has been suggested that typical HV and atypical HV are variants with the same disease spectrum of EBV-associated lymphoproliferative disorders, which ranges from typical HV as a benign pole to severe HV progressing to hematologic malignancy as a malignant pole. Chronic active Epstein-Barr virus infection (CAEBV) is an atypical illness associated with serologic evidence of persistent EBV infection. The extremely high levels of EBVmeasured in affected tissue or peripheral blood are the most recently proposed diagnostic criteria. I also found EBV associated HV-like eruption in the patients with CAEBV. During last 25 years, I have experienced more than 20 patients with EBV associated HV-like eruption. In this presentation, I will talk about various clinical features of the patients with EBV associated HV-like eruption that I have experienced.

‘후지’, ‘시나노 스위트’ 및 ‘갈라’ 품종에서의 약제적화

조광현(Kwang-Hyun Cho),한수곤(Su-Gon Han),윤태명(Tae-Myung Yoon) 한국원예학회 2011 한국원예학회 학술발표요지 Vol.2011 No.5

DWDM기반의 OVPN에서 네트워크 생존성을 위한 제어 메커니즘 연구

조광현,정창현,홍경동,김성운,Cho Kwang-Hyun,Jeong Chang-Hyun,Hong Kyung-Dong,Kim Sung-Un The Institute of Electronics and Information Engin 2005 電子工學會論文誌-TC (Telecommunications) Vol.42 No.9

A ' Virtual Private Network (YPN) over Internet' has the benefits of being cost-effective and flexible. However, given the increasing demands for high bandwidth Internet and for reliable services in a 'VPN over Intemet,' an IP/GMPLS over DWDM backbone network is regarded as a very favorable approach for the future 'Optical VPN (OVPN)' due to the benefits of transparency and high data rate. Nevertheless, OVPN still has survivability issues such that a temporary fault can lose a large amount of data in seconds, moreover unauthorized physical attack can also be made on purpose to eavesdrop the network through physical components. Also, logical attacks can manipulate or stop the operation of GMPLS control messages and menace the network survivability of OVPN. Thus, network survivability in OVPN (i.e. fault/attack tolerant recovery mechanism considering physical structure and optical components, and secured transmission of GMPLS control messages) is rising as a critical issue. In this Paper, we propose a new path establishment scheme under shared risk link group (SRLG) constraint for physical network survivability. And we also suggest a new logical survivability management mechanism by extending resource reservation protocol-traffic engineering extension (RSVP-TE+) and link management protocol (LMP). Finally, according to the results of our simulation, the proposed algorithms are revealed more effective in the view point of survivability. "인터넷을 기반으로 하는 VPN(Virt at Private Network)"은 비용과 운용측면에서 효율적이다 하지만 광 대역폭 그리고 신뢰성 있는 서비스에 대한 요구의 증가는 IP/GMPLS over DWDM 기반의 백본 네트워크가 차세대 OVPN (Optical VPN)을 위하여 가장 적합한 백본 네트워크로 간주되게 하였다. 그러나, 높은 데이터 전송율을 가지는 OVPN망에서 광 소자의 일시적인 fault/attack에 의해서 일어나는 서비스 파괴는 순식간에 막대한 트래픽 손실을 야기 할 수 있으며, 비 인가된 physical attack 으로 인하여 물리적인 구성소자를 통해 정보가 도청 될 수 있다 또한 데이터 전송을 관리하는 제어 메시지가 변조되거나 복사되어 조작될 경우 데이터가 전송도중 실패하더라도 망의 생존성을 보장할수가 없게 된다. 따라서, OVPN에서는 생존성 문제 (i.e. fault/attack에 대한 물리적인 구조와 광 소자를 고려한 최적의 복구 매커니즘, 그리고 GMPLS 제어메시지의 보안성 있는 전송) 가 중요한 이슈로 대두되고 있다. 본 논문에서는 fault/attack을 관리하기 위해 광 소자들과 공통된 위험 요소를 포함하는 소자들을 분류하고, SRLG (Shared Risk Link Group)를 고려한 경로 설립 스킴과 GMPLS의 RSVP-TE+(Reservation Protocol-Traffic Engineering Extension)와 LMP(Link Management Protocol)의 보안성 제공 메커 니즘을 제안하여, OVPN에서의 생존성을 보장한다. 끝으로 시뮬레이션 결과를 통하여 제안된 알고리즘이 망 생존성을 위하여 더욱 효율적임을 증명하였다.

베체트병의 결절홍반양 병변에 대한 병리조직학적 고찰

조광현,송계용,조미경,이유신,함의근 ( Kwang Hyun cho,Kye Yong Song,Mi Kyung Cho,Yoo Shin Lee,Eui Keun Ham ) 대한피부과학회 1988 大韓皮膚科學會誌 Vol.26 No.3

A histopathological study of erythema nodosum-like lesions in Behcet's disease was performed on 55 patients with incomplete and suspect types of Behcet's disease. Relatively common characteristics in histopathology of erythema nodosum-like lesions could be found in the patients with incomplete type of Behcet's disease. The histopathologic findings of erythema nodosum-like lesions in 21 patients with incomplete type of Behcet's disease were as follows : l. A moderate lymphocytic infiltration was found around the blood vessels and the sweat glands in the dermis. In the subcutaneous tissue, besides lymphohistiocytic infiltration, neutrophils were present in significant number in 9 cases (42.9%) 2. Vasculitis of small vessels was found within the periphery of fat lobules in 7 cases (81.0%), Five cases (23.8%) also showed thrombophlebitis. Seven cases (33.3%) showed histiocytic granuloma formation within the fat lobules. Microabscess formation in the subcutaneous tissue was seen in 5 cases (23.8 %), but it was not certain whether this was the charateristic feature.