선단 흑색종의 임상 및 병리조직학적 고찰

조광현(Kwang Hyun Cho),조미경(Mi Kyung Cho),이유신(Yoo Shin Lee),함의근(Eu Keun Ham) 대한피부과학회 1989 대한피부과학회지 Vol.27 No.4

A total of 68 primary melanorna cases diagnosed at Seoul National University Hospital for the past 10 years from 1978 to 1987 were included for this clinico pathological study. Among the 31 primary cutaneous melanomss, 19 cases (61.3%) occurred on hands or feet, especially on the plantar surfaces. The average age of patients with acral melanomas was 49.3 years at the tirne of diagnosis and the male to female ratio of 19 cases was 3.8 to 1. The clinical and histopathologic findings showed that most ecral melanomas, if not all, were the acral lentiginous type. A high incidence of metastasis (73.7%) was recognized in acral melanomas.

악성 피부 상피종양에서 유전분 침착

조광현(Kwang Hyun Cho),김규한(Kyu Han Kim),장승호(Seung Ho Chang),함의근(Eui Keun Ham) 대한피부과학회 1990 大韓皮膚科學會誌 Vol.28 No.6

We examined the amyloid deposit in various malignant cutaneous epithelial tumors by using Dylon stain. Histochemically identifiable amyloid deposits associated with basal cell epitheliomas(BCEs), squamous cell carcinomas(SCCs) and Bowens diseases were studied with monoclonal cytokeratin antibodies and anti-amyloid P antibodies. The results were as follaws, l. Amyloid deposits were detected in 5 of 12 BCEs, 4 of 11 SCCs, 3 of 9 Bowens diseases and 1 of 8 actinic keratoses. Amyloid deposits were not detected in o keratoacanthomas, 2 verrucous carcinomas, 1. Pagets disease and 3 extramammary Pagets diseases. 2. Anti-keratin antibody 34BE12 and anti-amyloid P antibody reacted with amyloid deposits in 3 of 5 BCEs which showed abundant amyloid deposits by Dylon stain. 3. Of 4 SCCs which showed amyloid deposits by Dylon stain, anti-keratin ant.ibody 34pE12 reacted with amyloid deposits in 2 cases and anti-amyloid P antibody reacted with amyloid deposits in 3 cases. 4. Anti-amyloid P antibody reacted with arnyloid deposits in 3 of 3 Bowens diseases which showed arnyloid deposits by Dylon stain. Anti-keratin antibody 34pE12 reacted with amyloid deposits only 1 of them. These findings suggest that epidermal keratins are the percursor substance of amyloid in malignant cutaneous epithelial tumors. The difference of antikeratin staining pattern between BCE-associated amyloid and SCC or Bowens disease-associated amyloid may be the results of difference in development stage of cutaneous amyloids. (Kor J Dermatol 28(6):715 121, 1990)

원발성 피부 유전분증에서 항케라틴 단세포군 항체를 이용한 유전분의 면역조직화학 염색

조광현(Kwang Hyun Cho),장승호(Seung Ho Chang),김정애(Jeong Aee Kim),이유신(Yoo Shin Lee),함의근(Eui Keun Ham) 대한피부과학회 1990 대한피부과학회지 Vol.28 No.3

Nine cases of primary localized cutaneous amyloidosis were studied by immunoperoxidase technique (ABC method) employing anti-keratin antibodies. All specimens were examined using consecutive paraffin sections to confirm the correspondence between amyloid existing area and reactive sites. Anti-keratin antibody 34pE which recognize 68, 58, 56.5, 56kd keratin peptides reacted with amyloid deposits in both lichen amyloidosus and macular amyloidosis. However, anti-keratin antibodies 34pB4 and 35pH did not react with amyloids. In general, Dylon staining positive material, keratin reacted with 34pE and amyloid P showed similar distribution in serial sections, but did not show the same one. Several keratin bodies reacted with 34pE, which were not stained with Dylon staining or antiamyloid P were found in the dermis of one specimen. These results suggest that immunohistochemical staining with antikeratin antibody 34pE using formalin-fixed, paraffin-embedded sections appeared to be a useful method in studying the histogenesis of primary localized cutaneous arnyloidosis. (Kor J Dermatol 28(3): 308 314, 1990)

멜라닌 세포성 병변에서 AgNOR 염색

조광현(Kwang Hyun Cho),김영걸(Young Gull Kim),이승철(Seung Chul Lee),함의근(Eui Keun Han) 대한피부과학회 1993 大韓皮膚科學會誌 Vol.31 No.4

Background : Histological differentiation between malignant melanoria and benign melanocytic skin lesions is at times, a difficult task for the dermatopathologist. The AgNOR staining has been regarded as a useful tool in differentiating malignant melanoma from benign rael;inocytic nevi. Objective : We have carried out the AgNOR staining in a range of nelanocytic lesion and try to assess the value of AgNOR stairting in the identification of malignancy in melanocytic lesions. Method : Fifty seven melainocytic skin specimens were studied. These comprised 11 acquired melanocytic nevi, 11 congenital melanocytic nevi, 31 malignant melano nas and 4 atypical melanocytic hyperplasias. Result : The majority of benign nevus cells posessed one or two unifrm AgNORs, whereas marked AgNOR pleomorphism was found in some rnelanoma cells. The number of AgNORs per nucleus. averaged 1.24+0.12 in the 18 specimens of benign nevi and 2.10+0.6 in the 25 specimens of malignant melanoma. In the cases of atypical melanocytic hyperplasia it was not possible to count on an adequate number of cells to give a meaningful result because of melanin pigment. Conclusion : Although this study demonstrated a separation of average AgNOR counts between begnign melanocytic nevi and maligmant melanomas, there was an ovei lap in counts among individual lesions. For clinical use, there should be a standard method by which AgNORs are counted in AgNOR staining. Melanin pigment masiking the AgNORs can also be a problem. (Kor J Dermatol 1993;31(4):532-538)

건선 피부병소에서 표피세포의 증식 및 분화 - 건선 표피에서 DNA 합성세포 및 분화 표식자 발현의 검색 -

조광현(Kwang Hyun Cho),장승호(Seung Ho Chang),김영걸(Young Gull Kim),윤재일(Jai Il Youn),박상철(Sang Chul Park),함의근(Eui Keun Ham) 대한피부과학회 1992 대한피부과학회지 Vol.30 No.3

DNA replicating cells were detected in the psoriatic epidermis by anti BrdU antibody after incubating tissue sections biopsied in acute and chronic areas with BrdU. The expression pattern of No. 1 keratin, involucrin. filaggrin, loricrin and transglutaminase E was studied in the paraffin embedded sections of psoriatic specimen which showed various histopathological findings. The results are as follows 1) The labeling index of BrdU in lesional psoriatic skin was significantly greater than that in normal skin. The labeling index was greater in the clinically active lesion of psoriasis than that of the chronic lesion, which suggest that psoriatic lesions are composed of distinct lesions differing in activity. 2) In the epidermis of the psoriatic plaques with extensive parakeratosis or microabscesses at the base of the stratum corneum and absent granular layers, the expression pattern of five epidermal proteins appeared as follows : No. 1 keratin which is found in normal epidermis immediately above the basal layer, appeared several layers higher. Involucrin was detected in most of the suprabasal layers. Fillaggrin, loricrin and transglutaminase E showed negative expression. The results obtained in the present study suggest that the increment of DNA replicating cells and aberrant, maturation pathway of epidermis appear in active psoriatic plaques. (Kor J Dermatol 1992; 30(3): 291-302)

피부 양성종양의 통계적 고찰

조광현(Kwang Hyun Cho),이유신(Yoo Shin Lee),함의근(Eui Keun Ham) 대한피부과학회 1985 大韓皮膚科學會誌 Vol.23 No.2

A statistical survey was made on 737 cases of cutaneous benign tumors that visited the Department of Dermatology at Seoul National University Hospital during a 11-year period from 1973 to 1983. The results were summarized as follows: 1) There were 329 cases of male(44. 6%) and 408 cases of female(55. 4%). The ratio of male to female was 1: l. 24. 2) Most patients with cutaneous benign tumors belonged to age ranged from 10 to 39 with the highest incidence in the 3rd decade. 3) According to Levers classification, 737 cases of cutaneous benign tumors were divided into following 6 groups: tumors and cysts of the epidermis(252 cases, 34, 2 tumors of the epidermal appendages(125 cases, 17.0%), tumors of fibrous tissue(98 cases, 13.3%), tumors of vascular tissue(158 cases, 21.4%), turnors of farty, muscular and osseus tissue(37 cases 5.0%), tumors of neural tissue(67 cases, 9. 1 %). 4) The distribution of the frequently observed cutaneous benign tumors was as fo]lows: epidermal cyst(12. 1%), nevus flammeus(11. 4%), neurofibroma(9, l%), linear epidermal nevus(7. 9%), syringoma(6, 6%), seborrheic keratosis(7. 9%.). nevus sebaceus(6. 0%), dermatofibroma(5. 8%), steatocystoma multiplex(4. 1%,), tuberous sclerosis(2. 8% ), granuloma pyogenicurn(2. 8%).

베체트병의 결절홍반양 병변에 대한 병리조직학적 고찰

조광현,송계용,조미경,이유신,함의근 ( Kwang Hyun cho,Kye Yong Song,Mi Kyung Cho,Yoo Shin Lee,Eui Keun Ham ) 대한피부과학회 1988 大韓皮膚科學會誌 Vol.26 No.3

A histopathological study of erythema nodosum-like lesions in Behcet's disease was performed on 55 patients with incomplete and suspect types of Behcet's disease. Relatively common characteristics in histopathology of erythema nodosum-like lesions could be found in the patients with incomplete type of Behcet's disease. The histopathologic findings of erythema nodosum-like lesions in 21 patients with incomplete type of Behcet's disease were as follows : l. A moderate lymphocytic infiltration was found around the blood vessels and the sweat glands in the dermis. In the subcutaneous tissue, besides lymphohistiocytic infiltration, neutrophils were present in significant number in 9 cases (42.9%) 2. Vasculitis of small vessels was found within the periphery of fat lobules in 7 cases (81.0%), Five cases (23.8%) also showed thrombophlebitis. Seven cases (33.3%) showed histiocytic granuloma formation within the fat lobules. Microabscess formation in the subcutaneous tissue was seen in 5 cases (23.8 %), but it was not certain whether this was the charateristic feature.

수종 피부 상피 종양에서 케라틴 발현에 관한 연구

조광현,김규한,장승호,이유신,함의근 ( Kwang Hyun Cho,Kyu Han Kim,Seung Ho Chang,Yoo Shin Lee,Eui Keun Ham ) 대한피부과학회 1990 대한피부과학회지 Vol.28 No.5

피지선모반에서 발생한 모종

이호균(Ho Gyun Lee),조광현(Kwang Hyun Cho),이유신(Yoo Shin Lee),김원석(Won Seok Kim),함의근(Eui Keun Ham),송계용(Kye Yong Song) 대한피부과학회 1990 대한피부과학회지 Vol.28 No.6

We reported a case of nevus sebaceus associated with trichilemmoma in a 19-year- old female patient. The lesion was a 2 x 2 cm sized yellowish verrucous plaque with two 0.3 x 0.3 cm sized dome-shaped papules. Histopathological examinat,ion revealed hyperkeratosis, papillomatosis and acanthosis, proliferation of sebaceus glands, presence of apocrine gland and mild inflammatory cellular infiltration around superficial vessels. In the epidermis of the lesion, there were two foci of lobulated mass consisted of clear cells with columnar basal cells and thick basement membrane. (Kor J Dermatol 28(6):818 821, 1990)

내부 장기암의 피부 전이 - 임상 및 병리조직학적 고찰 -

김유찬(You Chan Kim),조광현(Kwang Hyun Cho),이유신(Yoo Shin Lee),함의근(Eui Keun Ham) 대한피부과학회 1987 大韓皮膚科學會誌 Vol.25 No.2

We reviewed clinical records and histologic materials of 96 cases of metastatic skin cancer in Seoul National University Hospital from January 1973 to July 1986. The metastatic skin cancers of male were twice as many as that of female and they occurred most frequently in male at their fifties, female, forties. The most frequent primary cancers in men were carcinoma of the stomach, lung, colorectum and pancreas; in women, carcinoma of the breast, stomach, lung and uterine cervix. Cutaneous metastases from the carcinomas of the liver, uterine cervix and stomach which were many in Korea were smaller in number than that from the carcinomas of the lung and breast. Metastatic lesions were recognized before the primary tumor relatively often in carcinoma of the lung, pancreas and rarely in carcinoma of the uterine cervix, stomach. Though localizations of cutaneous metasta.sis were widespread over all body surfaces, regional areas of primary tumor were predisposed to metastasis. Metastatic carcinoma usually produced nonspecific nodules in the skin. Less commonly, some lesions of metastasis showed inflammatory or sclerotic plaque. The histologic findinga of cutaneous metastases generally mimicked that of primary tumors; nevertheless, in most instances, it was not possible to recognize the original tumor from a histologic examination of metastatic skin cancer and it was possible to classify the metastatic carcinoma only as an adenocarcinoma, a squamous cell carcinoma or an undifferntiated carcinoma.