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Kim, Ki Chan,Go, Hyo Sang,Bak, Hae Rang,Choi, Chang Soon,Choi, Inha,Kim, Pitna,Han, Seol-Heui,Han, So Min,Shin, Chan Young,Ko, Kwang Ho BioMed Central 2010 JOURNAL OF BIOMEDICAL SCIENCE -BASEL- Vol.17 No.1
<P><B>Background</B></P><P>Prenatal ethanol exposure during pregnancy induces a spectrum of mental and physical disorders called fetal alcohol spectrum disorder (FASD). The central nervous system is the main organ influenced by FASD, and neurological symptoms include mental retardation, learning abnormalities, hyperactivity and seizure susceptibility in childhood along with the microcephaly. In this study, we examined whether ethanol exposure adversely affects the proliferation of NPC and de-regulates the normal ratio between glutamatergic and GABAergic neuronal differentiation using primary neural progenitor culture (NPC) and <I>in vivo </I>FASD models.</P><P><B>Methods</B></P><P>Neural progenitor cells were cultured from E14 embryo brain of Sprague-Dawley rat. Pregnant mice and rats were treated with ethanol (2 or 4 g/kg/day) diluted with normal saline from E7 to E16 for <I>in vivo </I>FASD animal models. Expression level of proteins was investigated by western blot analysis and immunocytochemical assays. MTT was used for cell viability. Proliferative activity of NPCs was identified by BrdU incorporation, immunocytochemistry and FACS analysis.</P><P><B>Results</B></P><P>Reduced proliferation of NPCs by ethanol was demonstrated using BrdU incorporation, immunocytochemistry and FACS analysis. In addition, ethanol induced the imbalance between glutamatergic and GABAergic neuronal differentiation <I>via </I>transient increase in the expression of Pax6, Ngn2 and NeuroD with concomitant decrease in the expression of Mash1. Similar pattern of expression of those transcription factors was observed using an <I>in vivo </I>model of FASD as well as the increased expression of PSD-95 and decreased expression of GAD67.</P><P><B>Conclusions</B></P><P>These results suggest that ethanol induces hyper-differentiation of glutamatergic neuron through Pax6 pathway, which may underlie the hyper-excitability phenotype such as hyperactivity or seizure susceptibility in FASD patients.</P>
Ahn, Sang Jung,Bak, Hye Jin,Park, Ju Hyeon,Lee, Jin Young,Kim, Na Young,Han, Jin Woo,Jo, Hyae In,Chung, Joon Ki,Lee, Hyung Ho Humana Press 2013 Applied biochemistry and biotechnology Vol.170 No.5
<P>Cystatins are endogenous inhibitors of mammalian lysosomal cysteine proteinases, such as cathepsins B, L, H, and S. Cystatin C belongs to the type 2 cystatin family. In this study, the 751-bp cystatin C cDNA (PoCystatin C) of olive flounder (Paralichthys olivaceus) was cloned by screening from the olive flounder cDNA library. The mRNA expression of the PoCystatin C gene was examined in various tissues from normal and lipopolysaccharide (LPS)-stimulated olive flounder by RT-PCR and was compared with inflammatory cytokines IL-1β, IL-6, and IL-8. PoCystatin C transcripts ubiquitously existed in all normal and LPS-stimulated tissues that were tested. The recombinant PoCystatin C protein was expressed in Escherichia coli BL21(DE3) in pCold TF DNA expression vector as a 70-kDa fusion protein. The protease inhibitory activities of recombinant PoCystatin C toward papain cysteine protease, piscine cathepsins (L, S, K, F, and X), and bovine cathepsin B were measured with the synthetic fluorogenic peptide substrates. PoCystatin C tightly inhibited papain cysteine protease, whereas cathepsins L, S, K, F, X, and B were inhibited with lower affinities. Our results indicate that the P. olivaceus cystatin C is a homolog of mammalian cystatin C due to its sequence, structure, tissue expression, and biochemical activity.</P>
문맥압 항진과 식도 정맥류 출혈을 동반한 Agnogenic Myeloid Metaplasia 1예
장지정,오기원,김정아,최종영,문찬수,서정민,한석원,심규식,백남종,김부성 대한내과학회 1993 대한내과학회지 Vol.44 No.5
저자들은 식도정맥류 파열에 의한 위장관 출혈로 입원한 70세 여자환자에서 간경변과 문맥 혈전증 소견없이, 문맥압 항진과 심한 간, 비종대가 있으며, 골수 검사 및 비장 절제술 후 조직 검사결과 AMM으로 진단된 환자를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Agnogenic myeloid metaplasia is a syndrome in which the excessive fibroblast proliferation and collagen deposition in the bone marrow is accompanied by myeloid metaplasia of organ such as the liver, spleen, and lymph nodes. Typically, there is progressive splenomegaly, the gradual replacement of marrow elements by fibrosis, progressive anemla, variable changes in the number of granulocytes and platelets, and leukoerythroblastic peripheral blood picture. Here we report on a case of AMM associated with gastrointestinal bleeding due to ruptured esophageal varices. The portal vein was patent and the cause of portal hypertension was infiltration of liver by immature myeloid and erythroid cells and megakaryocytes.
디지털치료제 개념 및 VR을 활용한 잠수함 승조원 스트레스 완화 시스템
정준(Jun Jeong),이기원(Ki-Won Lee),박병호(Byeong-ho Bak),이승률(Seung-Ryool Lee),김만수(Man-soo Kim),차종한(Jong-Han Cha) 한국해군과학기술학회 2023 Journal of the KNST Vol.6 No.2
For submarines, which are the core power of the Korean Navy, it is important to nurture and maintain crew members that require a high level of expertise and experience. From 2016 to 2020, 712 people were trained, but 368 people left, so the outflow rate reached 51.6%. In this study, one of the reasons for the departure of the submarine crew was considered as stress, and a method of using VR as a way to relieve the crew members stress was studied.