c-형강 및 ebb & flow 방식에 따른 경제성 분석

강승원(Seung Won Kang),박천호(Chun Ho Pak),조용성(Yong Sung Cho) 한국원예학회 2007 원예과학기술지 Vol.25 No.3

관비재배와 저면관수인 ebb & flow, C-형강의 재배방식에 따른 고품질분화 생산을 위한 재배농가의 경제성을 분석하였다. 이를 위해 화훼농가의 비용구조를 분석하였고 자본 회수기간 및 내부수익률의 추정, 관수에 따른 인건비 절감 효과를 분석하여 재배방식에 따른 경제성을 비교하였다. 10a(991.7m²) 규모의 자동온실에 대해 관비재배방식을 선택한 시클라멘 재배농가의 총 비용은 9,993.8만원으로 추정되며, 이 중 시설비는 74%(7,410만원)를 점하고 연간 2,583.8만원의 경영비를 지출할 것으로 예상된다. 반면, C-형강방식을 선택한 재배농가는 초기 시설비용으로 약 8,520.0만원(총비용의 약 70%)을 지출하고, 매년 경영비로 3,597.0만원을 지출할 것으로 추정된다. 한편, 투자자금에 대한 자본회수 기간을 분석한 결과 10a 규모의 자동온실에서 시클라멘을 재배하는 농가에서 관비재배방식 혹은 C-형강방식을 선택할 경우에는 초기시설투자비 회수기간이 약 4 년 정도 소요될 것으로 예측되며, ebb & flow 방식을 선택할 경우에는 약 5년 정도의 시간이 소요될 것으로 예상된다. 또한, 내부 수익률을 추정한 결과 ebb & flow시설과 C-형강시설을 5년과 6년씩 사용한다고 가정할 경우, ebb & flow시설의 내부 수익률은 각각 9%와 14%로 나타났고, C-형강시설의 경우에는 각각 13%와 17%로 나타나 시중의 저축성예금 수신금리 4.82%와 비교하여 경제성이 높은 것으로 분석되었다. Economical efficiency for production of high quality-potted plants was analyzed by types of irrigation system such as conventional fertigation, ebb & flow, and C-channel as subirrigation systems. After cost structure was analyzed, payback period of capital, internal rate return (IRR), and effects of reduced labor cost were estimated. The gross cost of cyclamen-producing farmers using hydroponic systems in automated greenhouses (10a) was about 100 million won. Of the gross cost, installation and operation costs occupied 74% and 25%, respectively. In the C-channel subirrigation system, gross cost was 120 million won approximately. Installation and operation costs occupied 70% and 30%, respectively. Payback period of capital was 4 years for fertigated irrigation and C-channel subirrigation system, whereas it was 5 years for ebb & flow. IRR of ebb & flow and C-channel subirrigation systems was higher than the interest rates of 4.82%, indicating economic efficiency.

Impact of Interferon-Based Treatment on Quality of Life and Work Related Productivity from the Korean Cohort in the MOSAIC Study

( Sang Hoon Ahn ),( Won Hyeok Choe ),( Yoon Jun Kim ),( Jeong Heo ),( Dorota Latarska-smuga ),( Jiho Kang ),( Seung Woon Paik ) 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: Chronic Hepatitis C Virus (HCV) infection increases the risk for progressive liver disease, hepatocellular carcinoma and negatively impacts the patient’s quality of life. HCV treatment is evolving with direct acting antivirals but IFN based therapy has been the standard of care for many years and remains available in some countries. The MOSAIC study aims to characterize patients with chronic HCV infection and assess the impact of IFN-containing treatment on health-related quality of life, work related productivity and health care utilization. Methods: MOSAIC is an international prospective multicenter observational study that has been conducted in 20 countries. Consecutive patients with chronic HCV infection were enrolled and those who initiated an IFN based regimen were prospectively followed for 48 weeks. We report results from the Korean cohort Results: 100 patients were enrolled: 86 were treatment naïve and 14 were treatment experienced. 33 patients initiated an IFN based regimen: 6 patients started IFN + RBV, 26 patients started Peg-IFN + RBV, none started Peg-IFN + RBV + DAA and 1 patient received other treatment. Among the treated cohort, demographic and disease characteristics were the following: the mean age was 54.5 years; 14 patients were male. 14 had minimal or no fibrosis, 2 portal fibrosis, 3 bridging fibrosis and 6 patients suffered from cirrhosis. HCV Genotype distribution was as follows: genotype 1: 11; genotype 2: 19 and genotype 3: 3. Table 1 describes the results at baseline and changes over 4, 12 and 48 weeks and end-of-treatment (EOT) for the quality of life and work productivity outcome measures (EQ-5D-5L, HCV-PRO and WPAI). Conclusions: Results from the Korean cohort of the MOSAIC study show a moderate trend for deterioration of health-related quality of life and work productivity associated with IFN based treatment for patients with chronic HCV infection during treatment period. Acknowledgements: The design, study conduct, analysis, and financial support of MOSAIC study were provided by AbbVie. AbbVie participated in the interpretation of data, review, and approval of the content of the abstract. All authors had access to all relevant data and participated in writing, review, and approval of this abstract. Medical writing support was provided by Olivier Van de Steen of Medeor-consulting, funded by AbbVie. Disclosures: Sang Hoon Ahn: served as an advisor and lecturer for Bristol-Myers Squibb, Gilead Sciences, F.Hoffmann-La Roche, Merck, AbbVie, and has received unrestricted grants from Bristol-Myers Squibb, Gilead Sciences, and F. Hoffmann-La Roche for investigator- initiated trials Won Hyeok Choe: Nothing to disclosure Yoon Jun Kim: Nothing to disclosure Jeong Heo: received a grant from GSK; Research support from BMS, and Roche; Advisor for Abbvie, BMS, Gilead Sciences, Pharma Essentia, SillaJen, and Johnson & Johnson. Dorota Latarska-Smuga, Jiho Kang: are employees of AbbVie, Inc. and may hold stock or stock options. Seung Woon Paik: received grant and research support from AbbVie, BMS, Gilead, GSK, Merck, Novartis, and Roche

Characterization of F-Class Fly-Ash-Based Geopolymer Containing Various Cement Contents

Won, Ji Hwan,Kang, Seung Gu American Scientific Publishers 2017 Journal of Nanoscience and Nanotechnology Vol.17 No.6

<P>In this study, F-class fly ash-based geopolymers containing various amounts of Portland cement were fabricated by a high-pressure forming method and the compressive strength levels of the samples were assessed by analyzing the nano-microstructures and the types of phases which formed. In addition, two types of activators to harden the starting materials, water and a NaOH solution, were assessed to analyze how the nano-microstructures and the mechanical strength of the samples formed. When the specimen is hardened by water, the alkaline Ca(OH)(2) generated by the hydration of the cement induced a pozzolanic reaction of fine silica in the fly ash, thereby developing the mechanical strength of a specimen consisting of an admixture of fly ash and cement. When the specimen is hardened by the NaOH solution, the fly ash is geopolymerized while most of the constituents of the cement remain without hydration due to the low water content. With a nano-size structure, the compressive strength of the geopolymer was highest at 38 MPa when needle-shaped zeolite or calcium silicate hydrate (C-S-H) crystals of 50 similar to 150 nm in diameter were generated. This geopolymer containing 30 similar to 50% cement has potential as a substitution material for cement for applications in building materials given that its compressive strength measurement only after 1 day aging showed much higher value as 18 similar to 37 MPa compared to that of ordinary pure cement of below 10 MPa.</P>

Monitoring of veterinary drug residues in marin fish in 2016

Won Jo Choe,Mi Jung Park,Youngji Jung,Joon shik Park,Mikyoung Jin,Sookhee Ha,Gyeongyeol Kim,Eunhye Lee,Soo Yeon Choi,Seung Woo Kim,Dong-gil Lee,Kwang Soo Lee,Keum Soon Oh,Tae Seok Kang 한국분석과학회 2016 학술대회논문집 Vol.2016 No.11

Medicinal Chemistry : ERK1/2 antagonize AMPK-dependent regulation of FcεRI-mediated mast cell activation and anaphylaxis

( Seung Lark Hwang ),( Yue Lu ),( Xian Li Msc ),( Yong Deuk Kim ),( You Sook Cho ),( Yurn Dong Jahng ),( Jong Keun Son ),( Youn Ju Lee ),( Won Ku Kang ),( Yoshitaka Taketomi ),( Makoto Murakami ),( Ta 영남대학교 약품개발연구소 2015 영남대학교 약품개발연구소 연구업적집 Vol.25 No.-

Background: Extracellular signal-regulated kinases 1/2 (ERK1/2) make important contributions to allergic responses via their regulation of degranulation, eicosanoid production, and cytokine expression by mast cells, yet the mechanisms underlying their positive effects on FcεRI-dependent signaling are not fully understood. Recently, we reported that mast cell activation and anaphylaxis are negatively regulated by AMP-activated protein kinase (AMPK). However, little is known about the relationship between ERK1/2-mediated positive and the AMPK-mediated negative regulation of FcεRI signaling in mast cells. Objective: We investigated possible interactions between ERK1/2 and AMPK in the modulation of mast cell signaling and anaphylaxis. Methods: Wild-type or AMPKa22/2 mice, or bone marrow. derived mast cells obtained from these mice, were treated with either chemical agents or small interfering RNAs that modulated the activity or expression of ERK1/2 or AMPK to evaluate the functional interplay between ERK1/2 and AMPK in FcεRI-dependent signaling. Results: The ERK1/2 pathway inhibitor U0126 and the AMPK activator 5-aminoimidazole-4-carboxamide-1-b-4-ribofuranoside similarly inhibited FcεRI-mediated mast cell signals in vitro and anaphylaxis in vivo. ERK1/2-specific small interfering RNA also mimicked this effect on FcεRI signals. Moreover, AMPKa2 knockdown or deficiency led to increased FcεRI-mediated mast cell activation and anaphylaxis that were insensitive to U0126 or activator 5-aminoimidazole-4-carboxamide-1-b-4-ribofuranoside, suggesting that the suppression of FcεRI signals by the inhibition of the ERK1/2 pathway relies largely on AMPK activation. ERK1/2 controlled AMPK activity by regulating its subcellular translocation. Conclusions: ERK1/2 ablated the AMPK-dependent negative regulatory axis, thereby activating FcεRI signals in mast cells. (J Allergy Clin Immunol 2014;134:714-21.)

Rifampicin Alleviates Atopic Dermatitis-Like Response in vivo and in vitro

( Seung Hyun Kim ),( Ki Man Lee ),( Geum Seon Lee ),( Ju-won Seong ),( Tae Jin Kang ) 한국응용약물학회 2017 Biomolecules & Therapeutics(구 응용약물학회지) Vol.25 No.6

Atopic dermatitis (AD) is a common inflammatory skin disorder mediated by inflammatory cells, such as macrophages and mast cells. Rifampicin is mainly used for the treatment of tuberculosis. Recently, it was reported that rifampicin has anti-inflammatory and immune-suppressive activities. In this study, we investigated the effect of rifampicin on atopic dermatitis in vivo and in vitro. AD was induced by treatment with 2, 4-dinitrochlorobenzene (DNCB) in NC/Nga mice. A subset of mice was then treated with rifampicin by oral administration. The severity score and scratching behavior were alleviated in the rifampicin-treated group. Serum immunoglobulin E (IgE) and interleukin-4 (IL-4) levels were also ameliorated in mice treated with rifampicin. We next examined whether rifampicin has anti-atopic activity via suppression of mast cell activation. Rifampicin suppressed the release of β-hexosaminidase and histamine from human mast cell (HMC)-1 cultures stimulated with compound 48/80. Treatment with rifampicin also inhibited secretion of inflammatory mediators, such tumor necrosis factor-α (TNF-α) and prostaglandin D₂ (PGD₂), in mast cells activated by compound 48/80. The mRNA expression of cyclooxygenase 2 (COX-2) was reduced in the cells treated with rifampicin in a concentration-dependent manner. These results suggest that rifampicin can be used to treat atopic dermatitis.

Ordered Differential Display from Cryphonectria parasitica

Kang, Hyun-Seok,Choi, Jin-Won,Park, Seung-Moon,Cha, Byeong-Jin,Yang, Moon-Sik,Kim, Dae-Hyuk The Korean Society of Plant Pathology 2000 Plant Pathology Journal Vol.16 No.3

Ordered differential display using RT-PCR (ODD-PCR) was conducted to have a profile of the differently expressed genes between a hypovirulent strain of Cryphonectria parasitica (UEP1) and its isogenic wild type strain (EP155/2). ODD-PCR has advantages of high sensitivity, reproducibility, proportional representation, and limited number of primer combinations comparing with other differential display methods. RNAs were prepared from 1 and 5 day liquid culture of both hypovirulent and wild type strains, and were further evaluated with the marker genes of C. parasitica such as cryparin and mating factor MF2-1, which were already proven to be specifically down-regulated by the presence of mycovirus CHV1-713. ODD-PCR was conducted using those RNAs and expressed genes were categorized to five groups according to their temporal and quantitative expression patterns. Those fives groups are CPC, CPE, CPL, CPD, and CPU which represent constitutively-expressed, early-expressed, late-expressed, down-regulated, and up-regulated, respectively. Ninety two primer combinations out of a total of 192 have been tested so far. Among the twenty to fifty distinct bands per each reaction, an average of four to ten genes was identified as viral-regulated fungal genes. Those viral-specifc genes were further analyzed by DNA sequencing followed by homology search. Characterization of 30 clones including all five groups were conducted as a preliminary data and more are under investigation.

Ascorbic acid insufficiency induces the severe defect on bone formation via the down-regulation of osteocalcin production

Won Kim,Seyeon Bae,Hyemin Kim,Yejin Kim,Jiwon Choi,Sun Young Lim,Hei Jin Lee,Jihyuk Lee,Jiyea Choi,Mirim Jang,Kyoung Eun Lee,Sun G,Chung,Young-il Hwang,Jae Seung Kang,Wang Jae Lee 대한해부학회 2013 Anatomy & Cell Biology Vol.46 No.4

The L-gulono-γ-lactone oxidase gene (Gulo) encodes an essential enzyme in the synthesis of ascorbic acid from glucose. On the basis of previous findings of bone abnormalities in Gulo-/- mice under conditions of ascorbic acid insufficiency, we investigated the effect of ascorbic acid insufficiency on factors related to bone metabolism in Gulo-/- mice. Four groups of mice were raised for 4 weeks under differing conditions of ascorbic acid insufficiency, namely, wild type; ascorbic acid-sufficient Gulo-/- mice, 3-week ascorbic acid-insufficient Gulo-/- mice, and 4-week ascorbic acid-insufficient Gulo-/- mice. Four weeks of ascorbic acid insufficiency resulted in significant weight loss in Gulo-/- mice. Interestingly, average plasma osteocalcin levels were significantly decreased in Gulo-/- mice after 3 weeks of ascorbic acid insufficiency. In addition, the tibia weight in ascorbic acid-sufficient Gulo-/- mice was significantly higher than that in the other three groups. Moreover, significant decreases in trabecular bone volume near to the growth plate, as well as in trabecular bone attachment to the growth plate, were evident in 3- or 4-week ascorbic acid-insufficient Gulo-/-. In summary, ascorbic acid insufficiency in Gulo-/- mice results in severe defects in normal bone formation, which are closely related to a decrease in plasma osteocalcin levels.

Cancer Pain Control for Advanced Cancer Patients by Using Autonomic Nerve Pharmacopuncture

Kang, Hwi-Joong,Yoon, Jung-Won,Park, Ji-Hye,Cho, Chong-Kwan,Yoo, Hwa-Seung KOREAN PHARMACOPUNCTURE INSTITUTE 2014 Journal of pharmacopuncture Vol.17 No.3

Objectives: The purpose of this study is to report a case series of advanced cancer patients whose cancer pain was relieved by using autonomic nerve pharmacopuncture (ANP) treatment. ANP is a subcutaneous injection therapy of mountain ginseng pharmacopuncture (MGP) along the acupoints on the spine (Hua-Tuo-Jia-Ji-Xue; 0.5 cun lateral to the lower border of the spinous processes of vertebrae) to enhance the immune system and to balance autonomic nerve function. Methods: Patients with three different types of cancer (gastric cancer, lung cancer, colon cancer with distant metastases) with cancer pain were treated with ANP. 1 mL of MGP was injected into the bilateral Hua-Tuo-Jia-Ji-Xue on the T1-L5 sites (total 12-20 mL injection) of each patient's dorsum by using the principle of symptom differentiation. During ANP treatment, the visual analogue scale (VAS) for pain was used to assess their levels of cancer pain; also, the dosage and the frequency of analgesic use were measured. Results: The cancer pain levels of all three patients improved with treatment using ANP. The VAS scores of the three patients decreased as the treatment progressed. The dosage and the frequency of analgesics also gradually decreased during the treatment period. Significantly, no related adverse events were found. Conclusion: ANP has shown benefit in controlling cancer pain for the three different types of cancer investigated in this study and in reducing the dosage and the frequency of analgesics. ANP is expected to be beneficial for reducing cancer pain and, thus, to be a promising new treatment for cancer pain.

AKinematic Analysis of Various Movement Types in Sang-mo-dol-lee-gi

Kang Dong-Won,Choi Jin-seung,Tack Ji-Hoon,Kim Hyun-Soo 대한기계학회 2011 대한기계학회 춘추학술대회 Vol.2011 No.4