TNF-α 유전자형과 방광암과의 관계

정필두,김은정,엄민식,서정원,윤석중,김종석,이상철,김원재 충북대학교 의학연구소 2001 忠北醫大學術誌 Vol.11 No.2

연구목적: TNF-α는 일부 종양의 종양화 과정과 관련이 있는 것으로 알려져 있다. 본 연구는 TNF-α 발현에 영향을 미치는 TNF-α 촉진자 -308 부위의 유전적 다형성이 방광암과 관련이 있는지 유무를 알고자 시행하였다. 대상 및 방법: 유전자 분석을 위하여 환자 113명 및 대조군 109명으로부터 혈액을 채취하여 genomic DNA를 분리한 후 PCR-RFLP 및 direct DNA sequencing을 통하여 TNF-α유전자의 다형성을 조사하여 방광암의 발생, 병기 및 분화도와 비교 검토하였다. 결과: TNF-α 촉진자 -308 부위의 유전형은 대조군에서는 GG형이 83.5%(90례 및 GA형이 16.5%(19례)로 관찰되었으며 AA형은 없었다. 환자군에서는 GG 형이 85.4%(97례), GA형 및 AA형은 각각 13.1%(15례)및 0.8%(1례)에서 관찰되었다. 두 군 모두에서 GG형이 가장 많이 나타났으며 다음으로 GA형을 보이고 AA형은 1례의 방광암 환자에서만 관찰되었다. -308부위의 경우도 두 군 사이에 유전자형의 차이는 없었다(p=0.259) 분화도별 분포를 보면 grade I이 20례, grade II가 49례, grade Ⅲ은 34례였고 병기별로 표재성인 경우가 90례였으며 침윤성은 14례였다. 분화도가 나빠질수록 GA형이 증가하였다(p=0.04). 그러나 병기와 TNF-α promoter -308부위의 유전자형 사이에는 유의한 상관 관계가 없었다(p=0.123). 결론: 방광암 환자의 혈액에서 GA genotype이 관찰되는 경우, 분화도가 나쁠 가능성이 매우 높기 때문에 좀 더 적극적인 치료와 세밀한 추적관찰을 함으로써 방광암으로 인한 사망과 암의 진행을 예방할 수 있을 것으로 기대한다. Purpose : Tumor necrosis factor-alpha (TNF-α) is involved in tumorigenesis of several cancers as an endogenous tumor promoter. The purpose of this study was to investigate whether genetic polymorphism of TNF-α promoter region (-308) was associated with human bladder tumor. Materials and Methods: The DNA from 113 and 109 respective blood samples of bladder tumor Patients and control group was analyzed by PCR-based restriction fragment length polymorphism (RFLP) and direct DNA sequencing methods to characterize the genetic polymorphism of -308 promoter region of the TNF-α gene in bladder tumor patients. We compared the association of bladder tumor with genetic Polymorphism of TNF-α promoter region(-308) in relation to the stage, grade, recurrence and progressio. Results : Eighty-six percents(97/113) of bladder tumor patients and 83.5% (90/109) of control group showed genotype GG at -308 region of TNF-α. Difference in genetic variations of TNF-α promoter (-308) did not exist between bladder tumor patients and control group(p=0.259). Tumor grade was significantly related to the GA genotype (p=0.04). The higher is the grade in bladder tumor, the more frequent was the GA genotype. Tumor stage, recurrence and progression were not significantly associated with genetic polymorphism of TNF-α promoter region (-308). Conclusion: The GA genotype of TNF-a promoter region (-308) had a significant impact on TNF-α production and was related to higher grade tumor compared to GG genotype. TNF-α serum levels in bladder tumor patients were significantly higher than controls. These data suggested that TNF-α might involve the tumorigenesis of the bladder rather than treatment or prevention of bladder tumor.

駕莫灣內 細菌 Flora의 季節的 變動에 關한 硏究: 1. 病原 Vibrio와 Salmonella에 關하여

Suk U SHIN(申錫雨),Wun Wha JEONG(丁云華),Tai Jung KANG(姜泰中),Sung Koo KANG(姜聖求) 전남대학교 수산과학연구소 1992 수산과학연구소논문집 Vol.1 No.-

都市下水 및 工場廢水 流入으로 인한 生物棲息環境의 惡化로 生態界에 莫大한 支障을 招來하고 있는 駕莫灣海域의 微生物群集 특히 病原 Vibrio 와 病原腸內 細菌을 調査하여 이들 細菌과 水域生態界와의 相互 關聯性을 解析하고저 下水流入海域과 淸淨海域을 中心으로 調査하여 다음과 같은 結果를 얻었다. 駕莫灣內 海水溫度는 8月에 29.5℃ 가장 높았고 冬季에는 10℃이하였다. 病原 Vibrio의 水系에 따른 生菌數는 淸淨海域(A)이 5月에 出現하여 9月에 3.2×10²/ml로 나타났고 汚染海域(C,D)에서는 4月에 出現하여 7~9月에 1.2×10²~1.7×10³/ml였고 病原腸內細菌은 淸淨海域에서 6月, 汚染海域에서 3月에 出現하였으며 各各 9月에 1.3×10²/ml, 6.2×10²/ml의 生菌數가 檢出되었다. 分離菌株에 對한 同定結果는 病原 Vibrio 屬 139 菌株中 V.parahaemolyticus 가 48菌株로 62.3%, V. fluvialis 가 24菌株로 17.3% 同定되어 이들 두 菌株가 病原 Vibrio 屬中 優点種이었고 病原腸內細菌은 分離菌 122菌株中 Salmonella 屬이 43菌株로 35.2% 檢出되었고 이 가운데서 Sal. typhi 가 24菌株로 55.8%, Sal. paratyphi-C가 10菌株로 23.3%를 차지하였다. Kamak bay is the very important sea area in which have been producing not only fish but shellfish. But recently this bay is showing the tendency contaminated by domestic sewage and industrial waste-water. Therefore in order to investigate for bacteria in the contaminated environment to give what kind of influence on an ecosystem of microorganism, the distribution and bacteriological characteristics of the pathogenic Vibrio and Enterobacteriaceae were investigated as follows : The temperature of sea water in Kamak bay was the highest temperature when it was 29.5℃ in August and it was less than 10℃ during winter season. The viable cell counts of pathogenic Vibrio in the clean and the contaminated sea area appears for the first time in May and April, respectively. The former was 3.2×10² per ml in September and the latter was 1.2×10² 1.7×10³ per ml during July and September. Those of pathogenic Enterobacteriaceae appears in June in the clean sea area and in March in contaminated sea area. Each of them in both areas were 1.3×10²and 6.2×10²per ml in September. Showing the indentification result of 139 strains isolated into genus Vibrio from each samples, V.parahaemolyticus and V.fluvialis were 62.3% and 17.3%, respectively. Among 122 stains isolated into Enterobacteriaceae by MacConky agar, genus Salmonella was 35.2% and among this genus Sal.typi(55.8%) and Sal.paratyphi-C(23.3%) were the predominant species.

Enzymatic Hydrolysate from Non-pretreated Biomass of Yellow Poplar (Liriodendron tulipifera) is an Alternative Resource for Bioethanol Production

Jung, Ji-Young,Choi, Myung-Suk,Kim, Ji-Su,Jeong, Mi-Jin,Kim, Young-Wun,Woon, Byeng-Tae,Yeo, Jin-Ki,Shin, Han-Na,Goo, Young-Bon,Ryu, Keun-Ok,Karigar, Chandrakant S.,Yang, Jae-Kyung Korean Society of Forest Science 2010 한국산림과학회지 Vol.99 No.5

Enzymatic hydrolysate from non pre-treated biomass of yellow poplar (Liriodendron tulipifera) was prepared and used as resource for bioethanol production. Fresh branch (1 year old) of yellow poplar biomass was found to be a good resource for achieving high saccharification yields and bioethanol production. Chemical composition of yellow poplar varied significantly depending upon age of tree. Cellulose content in fresh branch and log (12 years old) of yellow poplar was 44.7 and 46.7% respectively. Enzymatic hydrolysis of raw biomass was carried out with commercial enzymes. Fresh branch of yellow poplar hydrolyzed more easily than log of yellow poplar tree. After 72 h of enzyme treatment the glucose concentration from Fresh branch of yellow poplar was 1.46 g/L and for the same treatment period log of yellow poplar produced 1.23 g/L of glucose. Saccharomyces cerevisiae KCTC 7296 fermented the enzyme hydrolysate to ethanol, however ethanol production was similar (~1.4 g/L) from both fresh branch and log yellow poplar hydrolysates after 96 h.

Anti-Cancer Effect of the Combination of Thiacremonone and Docetaxel by Inactivation of NF-κB in Human Cancer Cells

( Jung Ok Ban ),( Jin Suk Cho ),( In Guk Hwang ),( Jin Woo Nah ),( Wun Jae Kim ),( Ung Soo Lee ),( Dong Cheul Moon ),( Heon Sang Jeong ),( Hee Soon Lee ),( Bang Yeon Hwang ),( Jae Kyung Jung ),( Sang 한국응용약물학회 2009 Biomolecules & Therapeutics(구 응용약물학회지) Vol.17 No.4

경유의 윤활 성능 향상을 위한 식물유 기반 알칸올 아마이드의 합성

육정숙 ( Jung Suk Yuk ),김영운 ( Young Wun Kim ),유승현 ( Seung Hyun Yoo ),정근우 ( Keun Wo Chung ),김남균 ( Nam Kyun Kim ),임대재 ( Dae Jae Lim ) 한국공업화학회 2012 공업화학 Vol.23 No.4

초저유황 경유의 윤활성능을 향상시킬 목적으로 식물유 기반 알칸올 아마이드 유도체를 합성하여 윤활성능을 평가하였다. 알칸올 아마이드 유도체는 폐식물유(다크오일), 팜유, 코코넛유를 메탄올과의 연속 전이에스테르화 반응을 통하여 합성한 지방산 메틸에스테르와 디에탄올아민(DEA)의 아마이드화 반응을 행하여 합성하였다. 합성한 알칸올 아마 이드 유도체는 1 wt% 범위 내에서 초저유황 경유에 잘 용해되었으며, 이 유도체를 120ppm 포함한 초저유황 경유의 윤활성능을 HFRR법으로 측정하였다. 그 결과, 초저유황 경유의 마모흔의 직경이 581 um에서 아마이드 첨가 후 305∼ 323 um으로 현저히 작아져 초저유황 경유의 윤활성능을 향상하는 것으로 확인되었다. 한편, 식물유의 종류에 따른 마모흔의 차이는 크지 않아 알칸올 아마이드 유도체의 알킬기의 구조에 따른 윤활성능의 차이는 크게 나타나지 않았 다. 알칸올 아마이드 한 종류를 선정하여 첨가 농도에 따른 윤활성능을 평가한 결과, 농도에 따라 마모흔의 직경이 현저히 작아지는 결과를 얻었는데 이는 윤활성능이 첨가 농도에 따라 향상되는 것을 의미한다. To improve the lubricity of ultra low sulfur diesel, vegetable oil-based alkanol amide derivatives were prepared and their lubricity properties were studied. To synthesize the alkanol amides, we conducted the amidation reaction of diethaolamine High Frequency Reciprocating Rig (HFRR) and the fatty acid methyl esters, obtained by the continuous transesterification of methanol and several vegetable oil, such as soybean oil, palm oil and coconut oil. The synthesized amides were soluble in ultra low sulfur diesel in the concentration range of ca. 1 wt%; the lubricating properties of ultra low sulfur diesel containing 120ppm of amides were measured using an HFRR method. It was found that the wear scar diameter in the pure ultra low sulfur diesel decreased significantly from 581 um to 305∼323 um upon the addition of the amides, indicating that lubricating properties of the diesel were improved. On the other hand, the types of vegetable oils did not affect the wear scar diameters, implying that lubricating properties of the diesel did not depend strongly on the structures of alkyl groups of alkanol amide derivatives. When we measured the lubricating properties of the one type of diesels containing various amounts of alkanol amide, we observed that the wear scar diameter decreased drastically with increasing the amide concentration, meaning that the lubricity improved with the amide concentration.

Nicotinamide Inhibits Growth of Carcinogen Induced Mouse Bladder Tumor and Human Bladder Tumor Xenograft Through Up-Regulation of RUNX3 and p300

Kim, Wun-Jae,Lee, Jung-Won,Quan, Changyi,Youn, Hyung-Joon,Kim, Hwan-Mook,Bae, Suk-Chul Elsevier 2011 The Journal of urology Vol.185 No.6

<P><B>Purpose</B></P> <P>Acetylation of chromatin interacting proteins is central to the epigenetic regulation of gene expression. Various tumor suppressors are inactivated by abnormal epigenetic modification. A great deal of effort has been devoted to developing anticancer agents that reactivate silenced tumor suppressors by modulating chromatin structure. Studies show that histone deacetylase inhibitors can act as anticancer agents and several histone deacetylase inhibitors are currently in clinical trials. We noted that the tumor suppressor <I>RUNX3</I> is inactivated by promoter hypermethylation in human bladder cancer. We investigated whether reactivation of <I>RUNX3</I> could suppress bladder cancer development in an animal model.</P> <P><B>Materials and Methods</B></P> <P>We analyzed <I>RUNX3</I> reactivation and protein stabilization by a mild inhibitor of class III histone deacetylases, nicotinamide, by immunoprecipitation and immunoblot. Mouse bladder tumor was induced by N-butyl-N-(4-hydroxybutyl) nitrosamine. The effect of nicotinamide on <I>Runx3</I> methylation status and tumor growth was measured.</P> <P><B>Results</B></P> <P>Nicotinamide induced <I>RUNX3</I> expression at the transcriptional and posttranslational levels in a carcinogen induced mouse bladder tumor model and in human bladder tumor xenografts. Nicotinamide effectively inhibited the growth and progression of bladder tumors without decreasing body weight.</P> <P><B>Conclusions</B></P> <P>Results suggest that nicotinamide has preventive and therapeutic effects on tumorigenesis through multiple mechanisms of <I>RUNX3</I> expression up-regulation.</P>

Research Articles : Enzymatic Hydrolysate from Non-pretreated Biomass of Yellow Poplar (Liriodendron tulipifera) is an Alternative Resource for Bioethanol Production

( Ji Young Jung ),( Myung Suk Choi ),( Ji Su Kim ),( Mi Jin Jeong ),( Young Wun Kim ),( Byeong Tae Woon ),( Jin Ki Yeo ),( Han Na Sin ),( Young Bon Goo ),( Keun Ok Ryu ),( Chandrakant S. Karigar ),( J 한국임학회 2010 한국산림과학회지 Vol.99 No.5

Enzymatic hydrolysate from non pre-treated biomass of yellow poplar (Liriodendron tulipifera) was prepared and used as resource for bioethanol production. Fresh branch (1 year old) of yellow poplar biomass was found to be a good resource for achieving high saccharification yields and bioethanol production. Chemical composition of yellow poplar varied significantly depending upon age of tree. Cellulose content in fresh branch and log (12 years old) of yellow poplar was 44.7 and 46.7% respectively. Enzymatic hydrolysis of raw biomass was carried out with commercial enzymes. Fresh branch of yellow poplar hydrolyzed more easily than log of yellow poplar tree. After 72 h of enzyme treatment the glucose concentration from Fresh branch of yellow poplar was 1.46 g/L and for the same treatment period log of yellow poplar produced 1.23 g/L of glucose. Saccharomyces cerevisiae KCTC 7296 fermented the enzyme hydrolysate to ethanol, however ethanol production was similar (~1.4 g/L) from both fresh branch and log yellow poplar hydrolysates after 96 h.

Prevalence of Porcine Proliferative Enteropathy in Korea

Byeong Yeal Jung,Aeran Kim,Mi Hak Park,Hye Yeon Park,Chae Wun Bae,Suk Chan Jung 대한수의학회 2013 대한수의학회 학술대회발표집 Vol.2013 No.-

Esculetin inhibits cell proliferation through the Ras/ERK1/2 pathway in human colon cancer cells.

Park, Sung-Suk,Park, Sung-Kyu,Lim, Jung-Hyurk,Choi, Yung Hyun,Kim, Wun-Jae,Moon, Sung-Kwon National Hellenic Research Foundation 2011 ONCOLOGY REPORTS Vol.25 No.1

<P>Esculetin, a phenolic compound, has been shown to inhibit the growth of colon tumors in animal studies. However, the roles of signaling pathways and cell cycle regulation in the esculetin-induced inhibition of cancer cell growth, remain to be elucidated. The present study suggests a novel mechanism for the Ras/ERK1/2 pathway in esculetin-treated human colon cancer HCT116 cells. The treatment of cells with esculetin resulted in significant growth inhibition and G1 phase cell cycle arrest, which led to the down-regulation of cyclin and cyclin-dependent kinase (CDK) expressions. This G1 phase cell cycle arrest was associated with the up-regulation of p27KIP expression. In addition, ERK1/2 was activated by esculetin. The pre-treatment of cells with the MEK1/2-specific inhibitor, PD98059, blocked the p27KIP expression induced by esculetin. Blockage of the ERK1/2 function consistently prevented the inhibition of cell proliferation and decreased G1 phase cell cycle protein levels. Furthermore, Ras activation was increased by the esculetin treatment. Transient transfection of the dominant negative Ras (RasN17) mutant gene abolished both the ERK1/2 activity and p27KIP expression induced by esculetin. Finally, the overexpression of RasN17 suppressed the esculetin-induced reduction in cell proliferation and cell cycle proteins. In conclusion, these results indicate that the Ras/ERK1/2 pathway is mediated by the p27KIP1 induction, leading to a reduction in cyclin/CDK complexes in the esculetin-induced inhibition of colon cancer cell growth. Overall, these findings indicate that the molecular action of esculetin has therapeutic potential for the treatment of colon malignancies.</P>

Tumor Necrosis Factor Alpha 촉진자의 유전형이 방광암의 분화도에 미치는 효과

정필두,김원재,김은정,김종석,엄민식,이상철 大韓泌尿器科學會 2002 Korean Journal of Urology Vol.43 No.3