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Ryu, Jiyoon,Kim, Hyung Joon,Chang, Eun-Ju,Huang, Hao,Banno, Yoshiko,Kim, Hong-Hee Wiley (John WileySons) 2006 The EMBO journal Vol.25 No.24
<P>Sphingosine 1-phosphate (S1P), produced by sphingosine kinase (SPHK), acts both by intracellular and extracellular modes. We evaluated the role of SPHK1 and S1P in osteoclastogenesis using bone marrow-derived macrophage (BMM) single and BMM/osteoblast coculture systems. In BMM single cultures, the osteoclastogenic factor receptor activator of NF-kappaB ligand (RANKL) upregulated SPHK1 and increased S1P production and secretion. SPHK1 siRNA enhanced and SPHK1 overexpression attenuated osteoclastogenesis via modulation of p38 and ERK activities, and NFATc1 and c-Fos levels. Extracellular S1P had no effect in these cultures. These data suggest that intracellular S1P produced in response to RANKL forms a negative feedback loop in BMM single cultures. In contrast, S1P addition to BMM/osteoblast cocultures greatly increased osteoclastogenesis by increasing RANKL in osteoblasts via cyclooxygenase-2 and PGE(2) regulation. S1P also stimulated osteoblast migration and survival. The RANKL elevation and chemotactic effects were also observed with T cells. These results indicate that secreted S1P attracts and acts on osteoblasts and T cells to augment osteoclastogenesis. Taken together, S1P plays an important role in osteoclastogenesis regulation and in communication between osteoclasts and osteoblasts or T cells.</P>
Intracellular Delivery of p53 Fused to the Basic Domain of HIV-1 Tat
Jiyoon Ryu,최수영,박진서,이학주,Kyeong-Ae Kim,이재용,Kil Soo Lee 한국분자세포생물학회 2004 Molecules and cells Vol.17 No.2
p53 is a potent tumor suppressor inactivated in many cancers. In this study, the membrane permeability of the HIV-1 Tat basic domain was exploited to introduce functional p53 into cancer cells. We expressed and purified a p53 fusion protein with the HIV-1 Tat basic domain at its N terminus (Tat-p53), and examined its transduction profile and biological activity in cancer cells. Tat-p53 was efficiently delivered to both the cy-toplasm and nucleus of cells, and was transcriptionally active, as judged by the level of p21/WAF1 protein and of p21 promoter activity. Transduction of cells with Tat-p53 resulted in apoptotic cell death in both p53 positive and negative human tumor cell lines. These results suggest that Tat-p53 could be useful in cancer therapy.
Ryu, Gyuha,Lee, Jiyoon The Korean Society of Medical and Biological Engin 2021 의공학회지 Vol.42 No.6
The global market size of AI based SaMD for medical image in 2023 will be anticipated to reach around 620 billion won (518 million dollars). In order for Korean manufacturers to efficiently obtain CE marking for marketing in the EU countries, the paper is to introduce the recommendation and suggestion of how to reclassify SaMD based on classification rules of MDR because, after introducing the Regulation EU 2017/745, classification rules are quite modified and newly added compared to the Directive 93/42/EEC. In addition, the paper is to provide several rules of MDR that may be applicable to decide the classification of SaMD. Lastly, the paper is to examine and demonstrate various secondary data supported by qualitative data because the paper focuses on the suggestion and recommendation with a public trust on the basis of various secondary data conducted by the analysis of field data. In conclusion, the paper found that the previous classification of SaMD followed by the rule of MDD should be reclassified based on the Regulation EU 2017/745. Therefore, the suggestion and recommendation are useful for Korean manufacturers to comprehend the classification of SaMD for marketing in the EU countries.
파생상품 거래량이 국내 금융기관의 기업가치와 수익성에 미치는 영향
채지윤(Chae, Jiyoon),백재승(Baek, Jae-Seung),이재람(Lee, Jaeram),류두진(Ryu, Doojin) 한국산업경영학회 2014 경영연구 Vol.29 No.1
본 논문은 파생상품을 거래하는 주요 금융기관인 국내 금융투자업(증권사, 선물회사) 기업과 은행에 대해 파생상품 거래량이 기업가치 및 수익성에 미치는 영향을 분석하여, 파생상품 거래량이 은행업과 금융투자업에 대하여 상반된 영향을 미친다는 것을 확인하였다. 은행업의 경우에 파생상품 거래량이 수익성에 긍정적인 영향을 주는 것으로 나타났지만, 이와 반대로 금융투자업에 대해서는 유의하게 부정적인 영향을 미쳤다. 아울러 종류별 파생상품 거래량을 사용하여 분석한 결과에서 파생상품 거래량의 부정적 영향의 대부분이 장내선물 거래에 의한 것임을 확인하였다. 파생상품 거래량이 기업가치 및 수익성에 부정적인 영향을 미치는 것과 달리, 파생상품 거래이익에 대해서는 긍정적인 영향을 주는 것으로 나타났다. 특히 누적 파생상품 거래량이 많은 금융투자업 기업일수록 높은 수준의 파생상품 거래이익을 거두는 경향이 뚜렷하게 존재하여 파생상품 거래량에 전문성 향상으로 인한 효과가 존재한다는 것을 확인하였다. 그러나 파생상품 거래를 통한 거래이익이 기업 전체의 기업가치 및 수익성 개선으로는 이어지지 못하는 것으로 드러났다. This study examines how the trading volume of derivatives is associated with firm values and asset returns of the Korean financial institutions including banks and brokerage firms. By analyzing the behavior of abnormal returns (ARs) and returns on assets (ROAs), we find that the trading volume of derivatives positively affects ROAs of banks, but negatively affects ARs and ROAs of brokerage firms in a significant way. The trading volume of exchange-traded futures mainly contributes to the negative effect. Furthermore, the trading volume of derivatives has a positive effect on trading profits of derivatives. In particular, the positive effect is obvious when the cumulative trading volume of derivatives is considered as an explanatory variable. This implies that the learning effect is a major cause of this positive relationship, but is not enough to improve the profitability and/or the enterprise value of financial institutions.
Kang, Yoon-Tae,Kim, Young Jun,Bu, Jiyoon,Chen, Shilin,Cho, Young-Ho,Lee, Hyun Min,Ryu, Chun Jeih,Lim, Yoojoo,Han, Sae-Won Elsevier 2018 Sensors and actuators. B Chemical Vol.260 No.-
<P><B>Abstract</B></P> <P>We report a dual-immunopatterned microfluidic device for capturing both epithelial and mesenchymal circulating tumor cells (CTCs) simultaneously in a single device using the two antibodies of anti-epithelial cell adhesion molecule (EpCAM) antibody and newly developed anti-63B6 antibody. In addition to the conventional epithelial antibody of anti-EpCAM, our in-house produced anti-63B6 antibody targets mesenchymal stem cell-like cancer cells and intermediate cancer cells, thus overcoming limitation of the conventional single anti-EpCAM based CTC isolations. These two antibodies are immobilized to each top and bottom layer of the present device and the combination of two layers forms a circular chamber with the center inlet. The mutual complementary cell isolation through competing reaction in different velocity zones between mesenchymal and epithelial antibodies enables the precise screening and profiling of CTCs depending on their positivity and degree of epithelial/mesenchymal surface antigen expression, which may differ in compliance with disease status.</P> <P>From the experiments using epithelial and mesenchymal-like cancer cells, the present device captures 94.47% of cancer cells, which is substantially higher than the EpCAM-only-based method in terms of heterogeneous cancer cell isolation including mesenchymal–like cancer cells. Patient blood samples were employed to assess the clinical application of the present device for examining the patient status based on their CTC heterogeneity. The CTCs captured by both antibodies exhibit considerably varied expression profiles of epithelial protein including cytokeratin and EpCAM. The present device facilitates the isolation of heterogeneous subtypes of CTCs. These undiscovered heterogeneities would be helpful for precise analysis of CTCs to find their underlying meaning in cancer progression.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We present a dual-immunopatterned microfluidic device for capturing both epithelial and mesenchymal circulating tumor cells. </LI> <LI> Our in-house produced anti-63B6 antibody targets mesenchymal stem cell-like cancer cells and intermediate cancer cells. </LI> <LI> This device captures 94% of epithelial and mesenchymal cancer cells, significantly higher than previous anti-EpCAM methods. </LI> <LI> Our novel antibody combination facilitates isolation of EpCAM+, EpCAM- CTCs with non-defined CTC-like (EpCAM-/CK-) cells. </LI> <LI> Heterogeneous CTCs enriched by the present device are expected to elucidate the undiscovered roles of CTCs for cancer. </LI> </UL> </P>
Roh Yun Hwa,Lee Seunghun,Ryu Jeong Ah,Kim Yeo Ju,Kim Yeesuk,Bae Jiyoon 대한영상의학회 2021 Korean Journal of Radiology Vol.22 No.12
Objective: The aim of this study was to compare the diagnostic performances of digital tomosynthesis (DTS) and conventional radiography in detecting osteonecrosis of the femoral head (ONFH) using computed tomography (CT), as the reference standard and evaluate the diagnostic reproducibility of DTS. Materials and Methods: Forty-five patients (24 male and 21 female; age range, 25–77 years) with clinically suspected ONFH underwent anteroposterior radiography, DTS, and CT. Two musculoskeletal radiologists independently evaluated the presence and type of ONFH. The diagnostic performance of radiography and DTS in detecting the presence of ONFH and determining the types of ONFH were evaluated. The interobserver and intraobserver reliabilities of each imaging modality were analyzed using Cohen’s kappa. Results: DTS had higher sensitivity (89.4%–100% vs. 74.5%–76.6%) and specificity (97.3%–100% vs. 78.4%–83.8%) for ONFH detection than radiography. DTS showed higher performance than radiography in identifying the subtypes of ONFH with statistical significance in one reader (type 1, 100% vs. 30.8%, p = 0.004; type II, 97.1% vs. 73.5%, p = 0.008). The interobserver agreement was excellent for DTS and moderate for radiography (kappa of 0.86 vs. 0.57, respectively). The intraobserver agreement for DTS was higher than that of radiography (kappa of 0.96 vs. 0.69, respectively). Conclusion: DTS showed higher diagnostic performance and reproducibility than radiography in detecting ONFH. DTS may be used as a first-line diagnostic modality instead of radiography for patients suspected of having ONFH.