EMP3 Overexpression in Primary Breast Carcinomas is not Associated with Epigenetic Aberrations

Wei Zhou,Jinming Yu,Zheng Jiang,Xingang Li,Fenghua Xu,Yanbing Liu,Peie Wen,Li Kong,Ming Hou,Jinming Yu 대한의학회 2009 Journal of Korean medical science Vol.24 No.1

Epithelial membrane protein 3 (EMP3) is a trans-membrane signaling molecule with important roles in the regulation of apoptosis, differentiation and invasion of cancer cells, but the detailed is largely still unknown. We analyzed the mRNA levels and methylation statuses of EMP3 in 63 primary breast carcinomas and assessed their correlations with clinicopathologic variables. The expression of EMP3 mRNA in primary breast carcinomas was significantly higher than the expression of 20 normal breast tissues (p<10-7). EMP3 overexpression in breast carcinomas was significantly related to histological grade III (p=3.9×10-7), lymph node metastasis (p= 0.003), and strong Her-2 expression (p=3.3×10-6). Hypermethylation frequencies of EMP3 were detected in 36.5% of breast carcinomas by methylation-specific polymerase chain reaction. However, no significant correlations were found between methylation status of EMP3 and mRNA expression levels as well as other clinical parameters. In conclusion, EMP3 may be a novel marker of tumor aggressiveness. Overexpression of EMP3 in primary breast carcinoma is not associated with DNA methylation.

Genome-wide association analysis for yield-related traits at the R6 stage in a Chinese soybean mini core collection

Li Xiangnan,Zhou Yang,Bu Yuanpeng,Wang Xinfang,Zhang Yumei,Guo Na,Zhao Jinming,Xing Han 한국유전학회 2021 Genes & Genomics Vol.43 No.8

Background Soybean (Glycine max (L.) Merr.) is an economically important crop for vegetable oil and protein production, and yield is a critical trait for grain/vegetable uses of soybean. However, our knowledge of the genes controlling the vegetable soybean yield remains limited. Objective To better understand the genetic basis of the vegetable soybean yield. Methods The 100-pod fresh weight (PFW), 100-seed fresh weight (SFW), kernel percent (KP) and moisture content of fresh seeds (MCFS) at the R6 stage are four yield-related traits for vegetable soybean. We investigated a soybean mini core collection composed of 224 germplasm accessions for four yield-related traits in two consecutive years. Based on 1514 single nucleotide polymorphisms (SNPs), genome-wide association studies (GWAS) were conducted using a mixed linear model (MLM). Results Extensive phenotypic variation existed in the soybean mini core collection and signifcant positive correlations were shown among most of traits. A total of 16 SNP markers for PFW, SFW, KP and MCFS were detected in all environments via GWAS. Nine SNP markers were repeatedly identifed in two environments. Among these markers, eight were located in or near regions where yield-related QTLs have been reported in previous studies, and one was a novel genetic locus identifed in this study. In addition, we conducted candidate gene analysis to the large-efect SNP markers, a total of twelve genes were proposed as potential candidate genes of soybean yield at the R6 stage. Conclusion These results will be benefcial for understanding the genetic basis of soybean yield at the R6 stage and facilitating the pyramiding of favourable alleles for future high-yield breeding by marker-assisted selection in vegetable soybean.

Current utilization of waste biomass as filler for wood adhesives: A review

Jinming Liu,Yongfeng Li,Hailin Mo,Enjun Xie,Jianlin Fang,Weixing Gan 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.115 No.-

Filler is one of the most important additives for wood adhesives. The introduction of filler offers variousfunctions for wood adhesives, such as the increase of initial viscosity, the decrease of permeability, theimprovement of bonding strength, and the reduction of production cost. However, flour, a grain resource,is widely used as a filler that is added to polymers in the production of wood-based panels. As a result, alarge number of edible resources are wasted every year. Waste biomass that biomass residues are abandonedin nature are a promising filler that can be used to replace flour. The utilization of waste biomass asfiller for wood adhesives to prepare wood-based panels not only improves its mechanical strength, butalso reduces formaldehyde emissions. However, most waste biomass shows disappointing prepressingproperties. Advances in the use of waste biomass as an alternative filler for wood adhesives werereviewed. In addition, the current limitations and future prospects of waste biomass were discussed. Recycling waste biomass is a useful way to avoid the enormous amount of waste generated by differentindustries and control environmental pollution. It contributes to the practical development of circulareconomy.

Removal of Uranium from Uranium Plant Wastewater Using Zero-Valent Iron in an Ultrasonic Field

Jing Li,Libo Zhang,Jinhui Peng,Jinming Hu,Lifeng Yang,Aiyuan Ma,Hongying Xia,Wenqian Guo,Xia Yu 한국원자력학회 2016 Nuclear Engineering and Technology Vol.48 No.3

Uranium removal from uranium plant wastewater using zero-valent iron in an ultrasonicfield was investigated. Batch experiments designed by the response surface methodology(RSM) were conducted to study the effects of pH, ultrasonic reaction time, and dosage ofzero-valent iron on uranium removal efficiency. From the experimental data obtained inthis work, it was found that the ultrasonic method employing zero-valent iron powdereffectively removes uranium from uranium plant wastewater with a uranium concentrationof 2,772.23 mg/L. The pH ranges widely from 3 to 7 in the ultrasonic field, and theprediction model obtained by the RSM has good agreement with the experimental results.

Dynamics modeling and modal experimental study of high speed motorized spindle

Yunsong Li,Xiaoan Chen,Peng Zhang,Jinming Zhou 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.3

This paper presents a dynamical model of high speed motorized spindles in free state and work state. In the free state, the housing is modeled as a rotor with equivalent masses including bearing pedestals, motor stator and rear end cover. As a consequence, a double rotor dynamics can be modeled for high speed motorized spindles by a bearing element which connects the housing and bearing pedestals. In the work state, the housing is fixed and the system becomes a bearing-rotor dynamical model. An excitation-measurement test in the free state is designed to analyze the cross spectral density and auto spectral density of input and output signals. Then the frequency response function of system and coherence function of input and output signals which are used to analyze the inherent characteristics of the double-rotor model can be obtained. The other vibration test in the work state is designed to research the dynamical supporting characteristics of bearings and the effects from bearings on the inherent characteristics of the system. The good agreement between the experimental data and theoretical results indicates that the dynamical model in two states is capable of accurately predicting the dynamic behavior of high speed motorized spindles.

Synthesis and Hybridizations of Copper Microplates for Catalytic Applications

Yuhuan Li,Jinming Zhou 한국고분자학회 2022 한국고분자학회 학술대회 연구논문 초록집 Vol.47 No.1

Phase II Trial of Epigallocatechin-3-Gallate in Acute Radiation-Induced Esophagitis for Esophagus Cancer

Xiaoling Li,Ligang Xing,Yujun Zhang,Peng Xie,Wanqi Zhu,Xiangjiao Meng,Yinxia Wang,Lingling Kong,Hanxi Zhao,Jinming Yu 한국식품영양과학회 2020 Journal of medicinal food Vol.23 No.1

Acute radiation-induced esophagitis (ARIE) is among the most serious form of toxicities associated with definitive radiotherapy or chemoradiotherapy used for treatment of patients with esophageal cancer. Our preliminary phase I and II trials of lung cancer patients who received radiotherapy indicated epigallocatechin-3-gallate (EGCG) as a promising therapeutic option against ARIE. Therefore, we conducted a prospective phase II study to validate the efficacy and safety of EGCG in the treatment of ARIE. The patients who received chemoradiotherapy or definitive radiotherapy for treatment of esophageal cancer in the Shandong Cancer Hospital and Institute in China were enrolled for the present study. EGCG (440 μM) was administered with first onset of ARIE and then at weeks after final radiotherapy. The patients were monitored every week for dysphagia, Radiation Therapy Oncology Group (RTOG) score, and esophagitis-related pain. Moreover, tumor response and the effect on survival following the treatment were also evaluated. Comparison of the RTOG score in the first, second, third, fourth, fifth, and even sixth week after EGCG prescription and the first and second week after radiotherapy with baseline indicates a significant reduction. The tumor response rate was 86.3%. The overall survival rate in 1, 2, and 3 years was found to be 74.5%, 58%, and 40.5%. Oral administration of EGCG solution seems to be feasible for treating ARIE in patients with esophageal cancer who receive radiation therapy. EGCG might be an ARIE-reliever without compromising the efficacy of radiation therapy. A randomized study with a control group is needed for further evaluation.

Babeisa duncani infection alters gut microbiota profile in hamsters

Shangdi Zhang,Jinming Wang,Xiaoyun Li,Yanbo Wang,Yueli Nian,Chongge You,Dekui Zhang,Guiquan Guan 대한기생충학ㆍ열대의학회 2023 The Korean Journal of Parasitology Vol.61 No.1

The genus Babesia includes parasites that can induce human and animal babesiosis, which are common in tropical and subtropical regions of the world. The gut microbiota has not been examined in hamsters infected by Babesia duncani. Red blood cells infected with B. duncani were injected into hamsters through intraperitoneal route. To evaluate the changes in gut microbiota, DNAs were extracted from small intestinal contents, acquired from hamsters during disease development. Then, the V4 region of the 16S rRNA gene of bacteria was sequenced using the Illumina sequencing platform. Gut microbiota alternation and composition were assessed according to the sequencing data, which were clustered with >97.0% sequence similarity to create amplicon sequence variants (ASVs). Bacteroidetes and Firmicutes were made up of the major components of the gut microbiota in all samples. The abundance of Bacteroidetes elevated after B. duncani infection than the B. duncani-free group, while Firmicutes and Desulfobacterota declined. Alpha diversity analysis demonstrated that the shown ASVs were substantially decreased in the highest parasitemia group than B. duncani-free and lower parasitemia groups. Potential biomarkers were discovered by Linear discriminant analysis Effect Size (LEfSe) analysis, which demonstrated that several bacterial families (including Muribaculaceae, Desulfovibrionaceae, Oscillospiraceae, Helicobacteraceae, Clostridia UGG014, Desulfovibrionaceae, and Lachnospiraceae) were potential biomarkers in B. duncani-infected hamsters. This research demonstrated that B. duncani infectious can modify the gut microbiota of hamsters.

Protective effect of lncRNA CRNDE on myocardial cell apoptosis in heart failure by regulating HMGB1 cytoplasm translocation through PARP-1

Hui Chen,Jinming Liu,Bin Wang,Yongjun Li 대한약학회 2020 Archives of Pharmacal Research Vol.43 No.12

Long non-coding RNAs (lncRNAs) are boundup with the regulation of various diseases. Here, we probedinto the effect of lncRNA colorectal neoplasia differentiallyexpressed (CRNDE) on heart failure (HF). The pathologicalalterations and cell apoptosis of heart tissues were observedby hematoxylin–eosin and TUNEL staining. The viabilityor apoptosis of mouse myocardial cells HL-1 was tested byXTT or flow cytometry. The interaction between lncRNACRNDE and poly-ADP-ribose polymerase 1 (PARP-1) wasverified by RNA immunoprecipitation and RNA pull-down. The stability of the PARP-1 protein and the acetylation levelof high mobility group box-1 (HMGB1) were determined bycycloheximide-chase and immunoprecipitation, respectively. LncRNA CRNDE expression was decreased in HF micetissues and doxorubicin (Dox)-treated HL-1 cells, whereasPARP-1 and HMGB1 were increased. The overexpressionof lncRNA CRNDE restrained HL-1 cell apoptosis inducedby Dox. Moreover, the interaction between CRNDE andPARP-1 was corroborated, CRNDE negatively regulatedPARP-1 expression, and the overexpression of CRNDEreduced PARP-1 protein stability. In HL-1 cells, PARP-1positively regulated the acetylation level and cytoplasmtranslocation of HMGB1. CRNDE restrained Dox-inducedapoptosis in mouse myocardial cells via the PARP-1/HMGB1 pathway.

English Teaching Quality Evaluation Model Based on the Combined Dempster–Shafer (DS) Theory and Support Vector Machine (SVM)

Zou Xiaowei,Liu Jinming,Liu Yong,Li Hui,Yang Haidong 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.12