Manganese single-atom nanostructures for highly efficient tumor therapy

Jiaping Pei,Hui Dou,Chencai Liu,Tushuai Li 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.109 No.-

The single-atom attracts growing interests in various fields and provides a new strategy for tumor therapyby inspiring chemodynamic-photothermal therapy (CPT) effects owing to its excellent catalytic properties. We synthesize the Folic acid@single atomic manganese (Mn) nanoparticles (FA@SAMn NPs) withmesoporous carbon sphere structure by the coordination aided polymerization assembly method. Antitumor therapeutic effect studies were carried out in vitro and in vivo. This hierarchical nanostructurepossesses a high surface area, large pore, and rich N that provide higher catalytic activity, and it also exhibitsexcellent activity and stability in the oxygen reduction reaction. With its accumulation in the specifictumor microenvironment, the FA@SAMn NPs could generate reactive oxygen species (ROS), which wouldmediate a series of 4 T1 cell damage and inhibit tumor propagation (56% mortality rate). Moreover, theFA@SAMn NPs possess an excellent photothermal effect under near infrared-II (NIR-II) laser irradiation. This leads to a local hyperthermia situation in the tumor area and contributes to tumor inhibition (91%mortality rate). In sum, FA@SAMn catalysts effectively suppressed tumor growth and significantlyincreased the survival time. This work aims to provide new potential single-atom-coordinated carbonnetworks that possess efficient biocatalytic sites and photothermal effects, inspiring a series of advancesin ROS and photothermal-related biological applications across broad biomedical fields.

Coexposure of Environmental Concentrations of cis-Bifenthrin and Graphene Oxide: Adverse Effects on the Nervous System during Metamorphic Development of Xenopus laevis

Meng Li,Jiaping Zhu,Hua Fang,Mengcen Wang,Qiangwei Wang,Bingsheng Zhou 대한환경공학회 2019 대한환경공학회 학술발표논문집 Vol.2019 No.-

The Use of Toluidine Blue Staining Combined with Paraffin Sectioning and the Optimization of Freeze-thaw Counting Methods for Analysing Root-Knot Nematodes in Tomato

Xiaoman Li,Wenchao Zhao,Xiaoxuan Zhou,Jiaping Feng,Yingjian Gao,Xuehui Yao,Yang Liu,Jie Liu,Rui Yang,Fukuan Zhao,Shaohui Wang 한국원예학회 2017 Horticulture, Environment, and Biotechnology Vol.58 No.6

Root-knot nematodes (RKNs) (Meloidogyne spp.) are one of the most damaging crop pests and cause severeyield losses worldwide. Easier and more efficient methods are needed for assessing nematode infestations and hostplant resistance. In the present study, we combined toluidine blue staining and paraffin sectioning to observe theestablishment of giant cells (GCs; redifferentiated cells induced by nematode feeding) and the developmental status ofRKNs in tomato (Solanum lycopersicum). The number of RKNs was counted using a modified freeze-thaw countingmethod. Finally, three tomato lines with different levels of RKN resistance, but otherwise identical genetic backgrounds,were tested to verify the applicability of the combined method. The results suggested that using toluidine blue stainingcombined with paraffin sectioning and freeze-thaw counting accurately reflects the level of RKN resistance of tomato plants.

Changes of the Macrobenthos Community with Non-native Mangrove Rehabilitation (Kandelia obovata) and Salt Marsh Invasion (Spartina alterniflora) in Ximen Island, Zhejiang, China

Qiuxuan Wang,Li Song,Susana Agusti,Carlos Duarte,George Christakos,Jiaping Wu 한국해양과학기술원 2021 Ocean science journal Vol.56 No.4

Macrobenthos organisms are vital ecological components of intertidal wetlands. This work studied effects of non-native mangrove rehabilitation (Kandelia obovata) and salt marsh invasion (Spartina alterniflora) on macrobenthos communities in Ximen Island (Zhejiang, China). The macrobenthos communities were compared between rehabilitated K. obovata forests of five different stand ages (2-, 8-, 11-, 16-, and 60-year-old) and adjacent S. alterniflora marsh and non-vegetated mudflat. Compared to the mudflat, S. alterniflora increased the abundance, Shannon-Wiener diversity index (H'), and Margalef's richness index (d') of macrobenthos. Gastropoda abundance was high in the S. alterniflora marsh, whereas, malacostraca biomass was low. Within mangrove forests, macrobenthos abundance was positively correlated with the stand age of mangroves (Spearman Correlation, P < 0.05), but the corresponding H', d' and Pielou's evenness index (J') showed negative correlations. Gastropoda dominated over macrobenthos in the younger stands (2-11 years in age), whereas, phascolosomatidea and bivalvia were more abundant in the older stands (16 and 60 years in age). Noticeably, the highest biomass for malacostraca was recorded from the 11-year-old stand. Macrobenthos communities differed among habitats, with the divergence in community structure between the S. alterniflora marsh and mangrove stands increasing with mangrove stand age, to be significantly different for stands planted over 16 years ago. Since the 16-year-old stand exhibited similar macrobenthos features to the 60-year-old stand, we speculated that, in the case of macrobenthos, a rehabilitated K. obovata forest can reach its maturity level at a time period of about 15 years in Ximen Island.

Effects of Fermented Rapeseed Meal on Growth Performance and Serum Parameters in Ducks

Fazhi, Xu,Lvmu, Li,Jiaping, Xu,Kun, Qian,Zhide, Zhang,Zhangyi, Liang Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.5

A trial was performed to study the effects of feeding a diet containing solid-state fermentation rapeseed meal (FRSM) replaced soybean meal (SBM) on growth performance and serum biochemistry parameters of ducks and then to determine the appropriate proportion of soybean meal replacement. The 75% rapeseed meal and 25% blood meal were mixed and inoculated with the Lactobacillus plantarum and Bacillus subtilis. Over the 21-day fermentation, isothiocyanates were reduced from 72.7 to 14.1 mmol/kg. A total of 1,280 fifteen-day-old Cherry Valley ducks were randomly allocated into 4 dietary treatments, 4 replicate groups of 80 ducks each for a 30-day feeding trial. In four treatment groups, fermentation rapeseed meal replaced soybean meal at 0, 33, 67 or 100%, respectively. Results showed that feed intake of ducks fed 100% FRSM was greater (p<0.05) than SBM and partial FRSM in both the finishing period (31-45 d) and entire feeding period (15-45 d). Daily gain increased gradually in the three treatment groups with augmenting FRSM over in the whole study period. In the growing period (15-30 d), compared with the SBM group, phosphorus and calcium content in serum from the FRSM group was improved (p<0.05). Total protein concentration was lower in ducks fed 100% FRSM than SBM and 33% FRSM (p<0.05). Concentrations of IgM were dramatically higher for animals fed 100% FRSM than in the SBM, 33% FRSM and 67% FRSM groups. In the finishing trail stage (31-45 d), only serum IgG content in 100% FRSM group was improved (p<0.05). Therefore, rapeseed meal fermented with Lactobacillus plantarum and Bacillus subtilis is a promising alternative protein source and fermented rapeseed meal can completely replace soybean meal in duck diet and potentially reduce the cost of duck production.

Analysis of shear lag effect in the negative moment region of steel-concrete composite beams under fatigue load

Jinquan Zhang,Bing Han,Hui-bing Xie,Wu Tong Yan,Wangwang Li,Jiaping Yu 국제구조공학회 2021 Steel and Composite Structures, An International J Vol.39 No.4

Shear lag effect was a significant mechanical behavior of steel-concrete composite beams, and the effective flange width was needed to consider this effect. However, the effective flange width is mostly determined by static load test. The cyclic vehicle loading cases, which is more practical, was not well considered. This paper focuses on the study of shear lag effect of the concrete slab in the negative moment region under fatigue cyclic load. Two specimens of two-span steel-concrete composite beams were tested under fatigue load and static load respectively to compare the differences in the negative moment region. The reinforcement strain in the negative moment region was measured and the stress was also analyzed under different loads. Based on the OpenSees framework, finite element analysis model of steel-concrete composite beam is established, which is used to simulate transverse reinforcement stress distribution as well as the variation trends under fatigue cycles. With the established model, effects of fatigue stress amplitude, flange width to span ratio, concrete slab thickness and shear connector stiffness on the shear lag effect of concrete slab in negative moment area are analyzed, and the effective flange width ratio of concrete slab under different working conditions is calculated. The simulated results of effective flange width are compared with calculated results of the commonly used specifications, and it is found that the methods in the specifications can better estimate the shear lag effect in concrete slab under static load, but the effective flange width in the negative moment zone under fatigue load has a large deviation.

Influence of synergistic effect of phosphate on corrosion resistance of self-assembled superhydrophobic composite film on 6061 aluminum alloy surfaces

Likui Wang,Shichen Lu,Feng Xie,Hanyu Liu,Jiaping Li,Wei Shang,Zhe Zhang,Yuqing Wen 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.128 No.-

Aluminum (Al) alloys are used in a wide range of applications due to their advantages. However, theexisting corrosion protection methods have insufficient protection ability and cannot achieve longtermprotection. In this paper, reactive protective film on the surface of aluminum alloys using 1-hydroxyethyl-1,1-diphosphonic acid (HEDP) molecules. Then, Lauric acid/Graphene (LA/rGO) film is usedto prepared on the surface of HEDP film. SEM, XRD, EDS, Raman, XPS and WCA were conducted to analyzephysical and chemical features of coatings. The total resistance of the film reaches 1.0 1020 Xcm2, in asalt spray test using 5wt% NaCl as the corrosion medium, corrosion spots appeared on day 28 with a corrosioninhibition efficiency of 99.99%. Theoretical studies with quantum chemical calculations haveshown that HEDP/LA/rGO composite films can be adsorbed on Al alloy surfaces through P-O-Al and PO-C chemical bonding. The influence of phosphate synergistic effect on the corrosion resistance of theself-assembled superhydrophobic composite film on the surface of 6061 aluminum alloy is discussed. This study presents a scientific and innovative design concept to develop coatings with excellent corrosionprotection for aluminum alloy, and then extend their application to certain areas.

Prognostic Value of TP53 Mutation for Transcatheter Arterial Chemoembolization Failure/Refractoriness in HBV-Related Advanced Hepatocellular Carcinoma

Miao Xue,Yanqin Wu,Wenzhe Fan,Jian Guo,Jialiang Wei,Hongyu Wang,Jizhou Tan,Yu Wang,Wang Yao,Yue Zhao,Jiaping Li 대한암학회 2020 Cancer Research and Treatment Vol.52 No.3

Purpose This study aimed to investigate the clinicopathologic features and mutational landscape of patients with hepatitis B virus (HBV)–related advanced hepatocellular carcinomas (HCC) undergoing transcatheter arterial chemoembolization (TACE). Materials and Methods From January 2017 to December 2018, 38 patients newly diagnosed with HBV-related advanced HCC were enrolled in the final analysis. Their pathological tissues and corresponding blood samples before TACE treatment were collected for whole-exome sequencing. Response to TACE was evaluated at 1-3 months after two consecutive use of TACE. Predictive factors were analyzed by univariate and multivariate analyses in a bivariate Logistic regression model. Enrichment of related pathways of all driver genes were acquired using the gene set enrichment analysis (GSEA). Results Among 38 patients, 23 (60.5%) exhibited TACE failure/refractoriness. Patients with TACE failure/refractoriness showed higher frequency of TP53 mutation than their counterparts (p=0.020). Univariate and multivariate analyses showed that only vascular invasion and TP53mutation were significantly correlated with TACE failure/refractoriness in HBV-related advanced HCC. Of the 16 patients without vascular invasion, eight (50.0%) had TP53 mutations, and TP53mutation was associated with TACE failure/refractoriness (p=0.041). Moreover, GSEA showed that mitogen-activated protein kinase and apoptosis pathways induced by TP53 mutation were possibly associated with TACE failure/refractoriness. Conclusion Our study suggested that TP53 mutation was independently related with TACE efficacy, which may work via mitogen-activated protein kinase and apoptosis pathways. These findings may provide evidence to help distinguish patients who will particularly benefit from TACE from those who require more personalized therapeutic regimens and rigorous surveillance in HBV-related advanced HCC.