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반응표면 분석을 이용한 Salmonella enteritidis의 증식 효능 평가
홍정미,정효준,이홍근 한국환경과학회 2002 한국환경과학회지 Vol.11 No.12
This stidy was performed to investigate the single and combined effect of concentrations of garlic juice according to the pH and temperature on the growth of Salmonella enteritdis in brain heart infusion broth, and to develope Response surface model for the effect of concentrations of garlic extract. The results of electric conductibility of Salmonella enteritidis growing in the range of incubation temperature (25~42℃), PH(5.5~9.0) and concentration of garlic juice(0~0.8%) showed that a badge with high low D.T.value and concentration of garlic extract were significantly with D.T.value and concentration of garlic extract were significantly correlated with D.T.value (p<0.01). Growth of Salmonella enteritidis in the condition of 37℃ and pH7.0 presented the lowest D.T.value.
Jeong, Yu-Jeong,Shang, Yun,Kim, Beg-Hab,Kim, Sun-Young,Song, Jae-Hyo,Lee, June-Seung,Lee, Myeong-Min,Li, Jianming,Nam, Kyoung-Hee Korean Society for Molecular and Cellular Biology 2010 Molecules and cells Vol.29 No.3
BRI1-Associated kinase1 (BAK1), a five leucine-rich-repeat containing receptor-like serine/threonine kinase, has been shown to have dual functions: mediating brassinosteroid (BR) signaling and acting in the BR-independent plant defense response. Sequence analysis has revealed that BAK1 has two homologs, BAK7 and BAK8. Because BAK8 deviates from the canonical RD kinase motif, we focused on the functional analysis of BAK7. The expression pattern and tissues in which BAK7 appeared partially overlapped with those observed for BAK1. Expression levels of BAK7 increased in the bak1 mutant. Overexpression of BAK7 rescued the bri1 mutant phenotype, indicating that BAK7 can compensate for BAK1 in BR-mediated processes, especially in the absence of BAK1. However, root and hypocotyl elongation patterns of transgenic plants overexpressing BAK1 or BAK7 appeared to be different from the patterns observed in a BRI1 overexpressor. Furthermore, the sensitivity of transgenic plants overexpressing BAK7 to brassinazole, a biosynthetic inhibitor of brassinolide (BL), did not change compared to that of wild-type plants. In addition, we generated transgenic plants expressing BAK7 RNA interference constructs and found severe growth retardation and early senescence in these lines. Taken together, these results suggest that BAK7 is a component of the BR signaling pathway, with varying degrees of genetic redundancy with BAK1, and that it affects plant growth via BL-independent pathways in vivo.
Lee, Hyo Jeong,Yang, Dong-Hoon,Ryu, Yeon-Mi,Song, Miyeoun,Song, Ho June,Jung, Kee Wook,Kim, Kyung-Jo,Ye, Byong Duk,Byeon, Jeong-Sik,Choi, Eun Kyung,Yang, Suk-Kyun,Kim, Jin-Ho,Myung, Seung-Jae The Korean Academy of Medical Sciences 2013 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.28 No.8
<P>15-Hydroxyprostaglandin dehydrogenase (15-PGDH) is downregulated during the early stages of colorectal carcinogenesis. The aim of the present study was to investigate the potential role of 15-PGDH in normal-appearing colorectal mucosa as a biomarker for predicting colorectal neoplasms. We obtained paired tumor and normal tissues from the surgical specimens of 32 sporadic colorectal cancer patients. mRNA expression of 15-PGDH was measured using a quantitative real-time PCR assay. We evaluated the association between 15-PGDH mRNA expression in normal-appearing mucosa, the presence of synchronous adenoma, and the cumulative incidence of metachronous adenoma. The relative 15-PGDH expression of normal-appearing mucosa in patients with synchronous adenoma was significantly lower than in patients without synchronous adenoma (0.71 vs 1.00, <I>P</I> = 0.044). The patients in the lowest tertile of 15-PGDH expression in normal-appearing mucosa were most likely to have synchronous adenoma (OR: 10.5, <I>P</I> = 0.024). Patients with low 15-PGDH expression in normal-appearing mucosa also demonstrated more advanced stage colorectal cancer (<I>P</I> = 0.045). However, there was no significant difference in the cumulative incidence of metachronous adenoma according to 15-PGDH mRNA expression in normal-appearing mucosa (<I>P</I> = 0.333). Hence, 15-PGDH in normal-appearing colorectal mucosa can be a useful biomarker of field effect for the prediction of sporadic synchronous neoplasms.</P>
Hyo-Jung Kim,Iel-Yong Sung,Yeong-Cheol Cho,Min-Su Kang,Gyu-Jin Rho,June-Ho Byun,Won-Uk Park,Myeong-Gyun Son,Bong-Wook Park,Hyeon-Jeong Lee,Young-Hoon Kang 한국조직공학과 재생의학회 2019 조직공학과 재생의학 Vol.16 No.6
BACKGROUND: Enhancement and maintenance of the stemness of mesenchymal stem cells (MSCs) is one of the most important factors contributing to the successful in vivo therapeutic application of these cells. In this regard, threedimensional (3D) spheroid formation has been developed as reliable method for increasing the pluripotency of MSCs. Moreover, using a new protocol, we have previously shown that dental tissues of extracted wisdom teeth can be effectively cryopreserved for subsequent use as a source of autologous stem cells. The main purpose of this study is to analyze the stemness and in vitro osteogenic differentiation potential of 3D spheroid dental MSCs compared with conventional monolayer cultured MSCs. METHODS: In this study, MSC-characterized stem cells were isolated and cultured from long-term cryopreserved dental follicles (hDFSCs), and then 2D hDFSCs were cultured under 3D spheroid-forming conditions using a newly designed microchip dish. The spheroids (3D hDFSCs) thus produced were investigated and characterized with respect to stemness, MSC marker expression, apoptosis, cell cycle analysis, extracellular matrix (ECM) production, and osteogenic and adipogenic differentiation properties. RESULTS: In terms of MSC and senescence markers, spheroid cells showed no difference when compared with 2D hDFSCs; however, 3D hDFSCs were observed to have a higher proportion of cell cycle arrest and a larger number of apoptotic cells. Moreover, spheroids showed substantially increased levels of pluripotency marker (early transcription factors) and ECM protein expression. Compared with 2D hDFSCs, there was also a notable enhancement in the osteogenic induction potential of spheroids, although no differences were observed with respect to in vitro adipogenesis. CONCLUSION: To the best of our knowledge, this is the first study to demonstrate the application of a spheroid culture system for dental follicle-derived stem cells using a microchip dish. Although further studies are needed, including in vivo transplantation, the results obtained in this study indicate that spheroid hDFSCs derived from cryopreserved dental follicle tissues could be used as a valuable source of autologous stem cells for bone tissue regeneration.
June Bong Lee,한달무리,이형태,Seon Mi Wi,Jeong Hoon Park,Jung-woo Jo,조영재,Tae-Wook Hahn,Sunjin Lee,Byunghak Kang,Hyo Sun Kwak,Jonghyun Kim,Jang Won Yoon 대한수의학회 2018 Journal of Veterinary Science Vol.19 No.2
Herein, we report the pathogenic and phylogenetic characteristics of seven Shiga toxin (Stx)-producing Escherichia coli (STEC) isolates from434 retail meats collected in Korea during 2006 to 2012. The experimental analyses revealed that all isolates (i) were identified as non-O157STEC, including O91:H14 (3 isolates), O121:H10 (2 isolates), O91:H21 (1 isolate), and O18:H20 (1 isolate), (ii) carried diverse Stx subtypegenes (stx1, stx2c, stx2e, or stx1 + stx2b) whose expression levels varied strain by strain, and (iii) lacked the locus of enterocyte effacement (LEE)pathogenicity island, a major virulence factor of STEC, but they possessed one or more alternative virulence genes encoding cytotoxins (Cdtand SubAB) and/or adhesins (Saa, Iha, and EcpA). Notably, a significant heterogeneity in glutamate-induced acid resistance was observedamong the STEC isolates (p < 0.05). In addition, phylogenetic analyses demonstrated that all three STEC O91:H14 isolates were categorizedinto sequence type (ST) 33, of which two beef isolates were identical in their pulsotypes. Similar results were observed with two O121:H10pork isolates (ST641; 88.2% similarity). Interestingly, 96.0% of the 100 human STEC isolates collected in Korea during 2003 to 2014 wereserotyped as O91:H14, and the ST33 lineage was confirmed in approximately 72.2% (13/18 isolates) of human STEC O91:H14 isolates fromdiarrheal patients.
아메드장치에서 발생한 섬유성 조직 증식 소견 및 마이토마이신을 이용한 여과포교정술 결과
Hyo Chan Jeong,Won June Lee 대한검안학회 2022 Annals of optometry and contact lens Vol.21 No.1
Purpose: To report a case of fibrous tissue ingrowth after Ahmed valve implantation and bleb revision using mitomycin-C. Case summary: A 50-year-old man, who had received an Ahmed implantation 6 months prior, underwent bleb revision due to persistent high intraocular pressure, despite massage and multiple needling attempts. Bleb encapsulation over the entire valve plate was observed, and the bleb was gently dissected. Fibrous ingrowth over the entire valve system was confirmed. The broad stalk-like fibrous tissue was firmly adhered to the valve system. It was removed by pulling the connected bleb, taking care not to cut the stalk. Then, the long stalk-like fibrous ingrowth along the cleft between valve leaflets and main plate was removed, in the same manner. A sponge soaked with mitomycin-C (0.3 mg/mL) was instilled for 2 minutes, and the conjunctiva was sutured. Two weeks after the surgery, conjunctival suture was performed due to Ahmed valve exposure. Three weeks later, the Ahmed valve was removed due to re-exposure. Trabeculectomy surgery was performed due to uncontrolled intraocular pressure. Conclusions: There is a possibility of extensive fibrous tissue ingrowth after Ahmed valve implantation; thus, care should be taken when using mitomycin-C for bleb revision.