향신재료를 이용한 Oleoresin제조에 관한 연구 - 1. 고추 Oleoresin의 추출

배태진(Tae-Jin Bae),최옥수(Ok-Soo Choi),박재림(Jae-Rim Bahk),김무남(Mu-Nam Kim),한봉호(Bong-Ho Han) 한국식품영양과학회 1991 한국식품영양과학회지 Vol.20 No.6

고추의 효율적인 이용의 한 방안으로서 고추 oleoresin추출조건의 구명(究明)과 저장중의 품질변화에 대해서 실험하였다. 고추 oleoresin의 최적 추출용매로는 사용한 8가지 용매중에서 acetone이 가장 좋았으며, 분쇄한 건조고추의 입자크기는 100 mesh, 시료중량에 대한 용매의 혼합비율은 1 : 3(w/w) 및 추출온도는 25℃에서 가장 효과적으로 추출되었다. 추출수율을 기준으로 적정 추출시간은 5시간이였으며, 이상의 최적 추출조건에서의 oleoresin 수율은 18.7%였다. To improve the utilization of red pepper, the extracting conditions of oleoresin, such as kind of solvents, particle sizes of a sample, sample to solvent ratios, extracting temperatures and times, were studied. Among eight solvents used for oleoresin extraction from red pepper, the optimal solvent was acetone. The most appropriate particle size of red pepper powder, extracting temperature and mixing ratio of red pepper to acetone were 100 mesh, 25℃ and 1 to 3(w/w), respectively. The basis of yield in oleoresin extraction, optimum extracting time was about 5 hours. The yield of oleoresin under the above-mentioned conditions was 18.7%.

Expression of Rhamnosyltransferase derived from Pseudomonas aeruginosa EMS1 in Pseudomonas putida

Na-Eun Lee(이나은),Joung-Eun Lee(이정은),Mi-Sun Cha(차미선),Sun-Ja Cho(조순자),Jae-Rim Bahk(박재림),Sang-Joon Lee(이상준) 한국생물공학회 2004 한국생물공학회 학술대회 Vol.2004 No.10

서낙동강에서 분리된 남조 Microcystis의 처리상태에 따른 세균의 성장 및 형태변화

박재림,하경,권윤미 한국환경과학회 2003 한국환경과학회지 Vol.12 No.9

To investigate the interaction of bacteria and Microcystis isolated from a hypertrophic reservoir(Seo-Nakdong River), the response of five bacteria in relation to the different treatment of Microcystis and microcystin production by addition of dominant bacteria Staphylococcus sciuri were examined. Five bacteria (S. sciuri. S. capitis, S. epidermis, Pseudomonas aeruginosa and Aeromonas almonicida) were identified from the reservoir. In the experiment of bacterial response, two types of bacterial growth pattern were observed. All bacteria showed active growth in heated Microcystis-added media. Especially, three species of bacteria (S. sciuri. S. capitis and P. aeruginosa) among them showed active growth in live Microcystis-added media. In Microcystis response, increase of microcystin production showed when dominant bacteria. S. sciuri was added.

都市 市場地域 大氣中의 細菌에 對한 抗生物質 感受性 調査(釜山市)

박재림 新羅大學校 1977 論文集 Vol.5 No.-

Antibiotics susceptibilities were tested to get useful data for prevention food contamination and the treatment of infections such as septicemia. Tested were colony count, biochemical reaction for identification and resistant patterns to antimicrobial agents for 4 genus of bacteria isolated from the atmosphere of 5 markets in Busan on Aug. 1, 1976. The findings are as follows; 1. Out of the total colony count(1830), Pseudomonas was shown to be 55.5 per cent(1016). Staphylococcus 17 per cent(300), B. subtilis 1.9 per cent(35), Proteus spp. 0.1 per cent(3) and others 26 per cent(476). The area which showed the highest number of bacteria isolated was Bujeon market with 73.2 per cent while the lowest was Chungmoo market with 3.6 per cent and Oncheon, Bupyong, Changseon with 8.7 per cent, 8.6 per cent and 5.6 per cent, respectively. 37.3 per cent of colony was found in the morning(08:00), 33.7 per cent in the afternoon(16:00) and 29 per cent at noon. 2. Identified Staphlyococcus was resistant to antimicrobial agents except for chloramphenicol and sulfonamide, while Proteus spp. except for dihydrostreptomycin and tetracycline. Pseudomonas showed resistance to all agents, but B. subtilis showed susceptibilities except for tetracycline, penicillin and colistin.

홍삼 사포닌이 Aspergillus flavus 의 발육과 Aflatoxin 생산에 미치는 효과

박재림,박효진,송동숙 한국균학회 1985 韓國菌學會誌 Vol.13 No.3

The study was carried out to find effects of the saponins that were extracted from red ginseng on the growth of, aflatoxins production by, and protein patterns of Aspergillus flavus NRRL 3357. A. flavus with 10^6 conidia at grown at 30℃ for seven days on the enriched medium. Mycelial growth and pH changes of medium which cultured the mold, were similar to those of the control group. However, aflatoxin which produced by the mold was less than that of the control in all concentration of the saponin. To be more specific, 0.3% of the saponin inhibited production of aflatoxin B₁ and G₁ to the extent of 31.6 and 21% of the control. The protein peaks of A. flavus at the fourth day of the culture were shown high intensity near the level of 14,300 daltons. However, the mold which cultured in the medium containing the saponin showed low intensity of protein than that of the control group on all molecular weight.

낙동강 중 , 하류에서 식물플랑크톤과 세균의 계절적 동태

박재림,하경,손연주,주기재 한국환경과학회 2001 한국환경과학회지 Vol.10 No.4

Investigations were carried out to observe the seasonal succession of phytoplankton, and viable counts of bacteria from the mid to lower reaches of Nakdong River. An intensive monitoring was conducted from May to December 1999 biweekly at 6 sites in a main channel and 3 tributaries. Although there are several sites with high nutrient loading from the basin, all of study sites showed mesotrophic states owing to high discharge(June∼September). Relatively low algal biomass and CFUs(mean of chl. a, 12.3±11.5 ㎍/ℓ, CFUs : 1.8×10 exp (7)) were observed during the rainy season. The diatom population was dominant(over 85% of total community) year-long with peaks (Stephanodiscus hantzschii) in the fall and winter. Dominance of blue-green algal groups during the summer was not observed in the summer. Microorganisms peaked in the summer and fall (June∼September), affected mainly by the inputs of phytoplankton and nutrients. Biomass of phytoplankton and CFU counts were higher in the Kumho River than the other tributaries and main channel.

단체급식 관리에 관한 연구(Ⅱ) : 食單分布를 通한 through Menu Analysis

朴栽林,崔味愛 新羅大學校 1980 論文集 Vol.8 No.-

Menus from 20 facilities in which group food service was being done with nutritionist were analyzed to get useful data for better food service. Analyzed menus were those used in January, April, July and October in 1978 in Busan. The results are as follows: 1. The facilities provided calorie and nutrients more than what the survey of Nation's Nutrition has revealed or the amount recommended by Korea FAO Association as desirable. The calorie was supplied with 74.92 per cent of carbobydrate, 14.14 per cent of protein and 10.94 per cent of fat. 2. The least calorie was supplied in summer, and the least calorie and nutrients supplied to the middle-level of work among the three levels of work. 3. The food & descriptions such as group 1(cereal & grains), group 2(starch and starch roots) and group 4(legumes) showed negative correlation to the other groups but the rest showed positive correlation. Meanwhile, correlation between nutrients and food & descriptions did not go far beyond a common knowledge. 4. Nutrients intakes provided by each facility was more than the amount recommended by Korea FAO Association except for Fe in 4 facilities and vitamin A in 8 facilities. 5. Animal protein got 31.14 per cent among the whole protein intakes, and fish & shell fish was the main source of animal protein. 6. In summer, less calorie was supplied in 4 facilities than the amount recommended by Korea FAO Association.

釜山市 大氣汚染에 關한 調査

朴裁林 新羅大學校 1975 論文集 Vol.2 No.-

Air pollutants were measured to get usefull data in preventing and controlling the pollution at industrial & semi-industrial, commercial, cross-road and residential areas in Busan from September 1973 to February 1974 Tested were dustfall(Ton/㎢/Month) by Deposit gauge method, sulfuric anhydride(mg SO3/ day/100㎠pb02) by Lead Peroxide Candle method and relation between the pollution and metero-logical factors was discussed. 1. The mean value of dustfalls was 24.8 tons/㎢/month ranging from 12.5 to 44.5 tons/㎢/month. The highest amount of dustfalls of 28.5tons/㎢/month was measured in cross-road areas while the lowest of 1.96 in residential areas and December the highest of 32.6 while in January the lowest 19.4 2. The mean value of water soluble substances was 31.7 percent and the highest percent of 38.8 in commercial areas while the lowest of 26.7 in cross-road areas. 3. Seasonal variation of pH was shown as 4.20 in autumn and 2.76 in winter. 4. The mean value of sulfuric anhydride was 1.248mg SO3/day/100㎠PbO2 ranging from 0.197 to 4.162mg SO3/day/100㎠Pb02. The highest concentration of sulfuric anhydride of 1.930mg SO3/dayday/100㎠Pb02 was detected in cross-road areas whil the lowest of 0.815 in residential areas and December the highest of 1.877 while in February the lowest of 0.647 5. Dustfalls and concentrations of sulfuric anhydride are more with the decreased of relative humidity and precipitation and temperature.

廚房空氣中에서 分離된 黃色葡萄狀球菌의 抗生物質에 대한 感受性硏究(Ⅱ)

朴栽林 新羅大學校 1981 論文集 Vol.11 No.-

Susceptibility of Staphylococcus aureus to antibiotics was tested to get data for prevention food contamination and treat for food poisoning. Tested were total colony counts, isolation of Staphylococcus aureus, resistant patterns to antibiotics and significant difference between isolation of the strains and the circumstances of kitchens. Staphylococcus aureus and other strains isolated from the atmosphere of 32 kitchens in the morning(at 7:00~8:00) on June 30, 1978 and 37 on September 3. 1970 in Busan. The findings are as follows: 1. The average of colony counts was shown to be 7.8 ranging from 53 to 1 in 1980 and 14.9 ranging from 104 to 1 in 1978. 2. Staphylococcus aureus were isolated from 5 kitchens with 13.5 per cent among 37 kitchens in 1980 and 5 kitchens with 15.6 percent among 32 in 1978. 3. The Staphylococcus aureus were susceptible to colistin, erythromycin, sulfonamide, and tetracycline, while the strains were resistant to neomycin, novomycin and streptomycin, and intermediate resistant to kanamycin, leucomycin, oleandomycin, penicillin and teramycin. 4. There was no significant difference(p>0.1) between the detection of Staphylococcus aureus and the kitchen circumstances such as, window size, kitchen-area ratio to floor-space, kitchen style, setting up fan or draft system, etc. According to this, if I give a guess, the detection of Staphylococcus aureus might be due to the human carrier rather than physical environments.

Listeriosis and Listeria monocytogenes

Bahk, Jae Rim,Marth, Elmer H. 한국산업미생물학회 1989 한국미생물·생명공학회지 Vol.17 No.6

Listeria monocytogenes, one of five species in the genus Listeria and the only one currently believed to be pathogenic for humans, is a small gram-positive, nonsporeforming, aerobic, motile and hemolytic rod-shaped bacterium. The bacterium is widespread in the environment, having been isolated from soil, dust, animal fee, water, sewage, almost every type of animal that has been cultured, and asymptomatic humans. L. monocytogenes causes listeriosis, a disease which most often affects humans with a compromised immune system. Included are pregnant woman, infants and adults suffering from such diseases as cancer, cirrhosis of liver of AIDS or are being treated with drugs such as corticosteroids. Listeriosis is manifested by such syndromes as pregnancy infections, granulomatosis infantiseptica, sepsis, meningoencephalitis, and focal infections. Infections, can be treated successfully with penicillin, ampicillin, or erythromycin. However, a mortality rate of about 30% has occurred in outbreaks of listeriosis. Food-associated outbreaks of listeriosis have been attributed to coleslaw (Canada, 1981), pasteurized milk (U.S., 1983), and soft cheese (U.S., 1985). Presence of L. monocytogenes in various dairy foods has prompted recall of such products from the U.S. market-place. L. monocytogenes also has been found in raw meats and seafood.