12. 홍삼 조 Saponin 의 Aspergillus flavus NRRL 3357 의 발육과 Aflatoxin 대사에 미치는 효과

박재림,박효진 한국균학회 1985 韓國菌學會誌 Vol.13 No.2

미생물 증식에 미치는 Soft silicate의 영향

박재림,박중철 신라대학교 자연과학연구소 2003 自然科學論文集 Vol.12 No.-

한국 Refresh 연구회로부터 제공받은 Soft silicate를 이용하여 미생물의 증식을 측정한 결과 배지첨가농도 0.5%로 대장균의 증식이 대조군의 약 3배 이상 촉진되었으며, L. casei는 대장균과 달리 0.1% 및 0.5%에서는 증식이 거의 관찰되지 않았으나 1% 및 3%첨가에서는 간헐적으로 대조군을 초과하는 증식이 관찰되었다. S. aureus는 시료 첨가농도 0.1%에서 최대증식을 나타냈고, 0.5%, 1% 순으로 각각 4배, 2배의 촉진효과가 나타났으며, V. parahaemolyticus는 첨가농도 0.1%가 약 3배, 0.5% 및 1%가 약 2배의 증식을 촉진시키는 추세를 나타냈지만, C. utilis 및 S. cerevisiae의 증식은 시료첨가 전 농도에서 증식정도가 대조군에 미치지 못하였다. The effect of soft silicate offered from Refresh Research Korea on growth of several microorganisms were tested. Growth of E. tali was stimulated more than 3 times of the control group when 0.5% soft silicate added in the broth. Growth of L. casei was not observed at the concentration of 0.1% and 0.5%. However, the growth of the organism was observed more than that of the control when 1% and 3% of the soft silicate added in the broth. Great peak showed at the concentration of 0.1% added in S. aureus and V. parahaemolyticus. Growth of C. utilis and S. cerevisiae were not reached to the growth of control group in all concentration added.

진균류의 증식과 대사에 미치는 방아(Agastache rugosa ) 추출물과 Estragole 의 효과

박재림,박송희,김정옥,김수원,이수영 한국식품위생안전성학회 1997 한국식품위생안전성학회지 Vol.12 No.1

The extracts from Agastache rugosa O. Kuntze, their chloroform and hexane fractions, and estragole identified from hexane fraction were tested to investigate the effects on the growth and metabolic activities of several true fungi. The fungi used were: Aspergillus oryzae KFCC 890, Aspergillus niger KCCM 11240, Saccharomyces cerevisiae IAM 459?, Saccharomyces ellipsoideus PNU 2215. The growth of S. cerevisiae by treatment of water extract (1%), hexane fraction (0.05%), and estragole (0.05%) were inhibited 93%, 50%, and 33% respectively, and S. ellipsoideus was also inhibited markedly with delaying the lag phase maximum 12 hrs. The growth of A. oryzae was inhibited by treatment of extracts and fractions. The ethanol production by S. cerevisiae was increased more than two times in the highest value around 42 hrs incubation by water extract, but chloroform fraction inhibited its production. The glucoamylase activities by A. niger were strongly inhibited by hexane and chloroform fractions (0.05%). The invertase activity by S. cerevisiae using estragole (0.05%) reached to 57.5% of control group. S. cerevisiae treated with the estragole was damaged the cell wall and cell membrane, leaked the protoplasm, and observed broken pieces of cell.

주방공기중에서 분리된 포도상구균의 함생물질에 대한 감응성연구(1)

박재림 한국환경보건학회 1978 한국환경보건학회지 Vol.5 No.1

Antibiotics susceptibilities of Staphylococci were tested to get useful data for prevention food contamination and treatment of food poisoning. Tested were colony counts, isolation of Staphylococci, identification for the Staphylococcus aureus and resistant patterns to antimcrobial agents. The colonies and Staphylococci isolated from the atmosphere of 37 home kitchens in the morning (07:00-08:00) on Nov. 19, 1977 in Busan. The findings are as follows: 1. The average of colony counts was shown to be 9.6 ranging from 47 to 1 at mean temperature of 18$\circ$C (the highest 21$\circ$C, the lowest 15$\circ$C) 2. Out of total 37 kitchens, 18 Staphylococci were isolated from 15 kitchens with 40.5 per cent, and 4 Staphylococcus aureus were identified with 22.2 per cent from 18 Staphylococci. 3. Isolated Staphylococci were resistant to penicillin(100%), dihydrostreptomycin(93.3%), tetracycline (93.3%) and oleandomycin(66.6%), while the strains were sensitive to sulfonamide(88.6%) and colistin(83.3%). 4. Staphylococcus aureus identified were resistant to dihydrostreptomycin, penicillin and tetracycline, while the strains were sensitive to sulfonamide and colistin.

집단급식 관리에 관한 연구(Ⅲ) : 영양사에 대한 기초조사

박재림 대한영양사회 1980 國民營養 Vol.20 No.-

수직 및 수평 단열판에 부착된 등온 사각비임에서의 자연대류 열전달

박재림,권순석 대한기계학회 1992 대한기계학회논문집 Vol.16 No.1

Laminar natural convection heat transfer from an isothermal rectangular beam attached to horizontal and vertical adiabatic plates has been studied for various aspect ratios of the beam and Grashof numbers. The local Nusselt number was increased with decreasing H/B for the constant beam width, B, and with increasing H/B for the constant beam height, H. The total mean Nusselt number of the vertical type was higher than that of horizontal type in the range of H/B.leq.0.52, but reversed in the H/B>0.52 at constant beam width. The total mean Nusselt number of the horizontal type was generally higher than that of vertical type at constant beam height. The total mean Nusselt number of the vertical type was higher than that of horizontal type in the range of H/B.leq.0.43 at constant wetted perimeter, but reversed in the H/B$\leq$0.43. 본 연구에서는 주위유체가 공기인 정상 층류 상태하에서 수직 및 수평단열판 에 부착된 등온 사각비임에서의 자연대류열전달 현상을 비임의 현상비와 Grashof수를 변수로 하여 고찰 하였다.

서낙동강에서 분리된 남조 Microcystis의 처리상태에 따른 세균의 성장 및 형태변화

박재림,하경,권윤미 한국환경과학회 2003 한국환경과학회지 Vol.12 No.9

To investigate the interaction of bacteria and Microcystis isolated from a hypertrophic reservoir(Seo-Nakdong River), the response of five bacteria in relation to the different treatment of Microcystis and microcystin production by addition of dominant bacteria Staphylococcus sciuri were examined. Five bacteria (S. sciuri. S. capitis, S. epidermis, Pseudomonas aeruginosa and Aeromonas almonicida) were identified from the reservoir. In the experiment of bacterial response, two types of bacterial growth pattern were observed. All bacteria showed active growth in heated Microcystis-added media. Especially, three species of bacteria (S. sciuri. S. capitis and P. aeruginosa) among them showed active growth in live Microcystis-added media. In Microcystis response, increase of microcystin production showed when dominant bacteria. S. sciuri was added.

Saponin이 Aspergillus parasiticus의 발육과 Aflatoxin생합성에 미치는 효과

박재림,임광식,이종근 한국미생물학회 1985 미생물학회지 Vol.23 No.4

Aspergillus parasiticus의 발육과 afla toxin생산에 미치는 생약 extract의 효과에 따라 선정된 생약 금은화, 우방자 및 인삼의 조 saponm이 군체의 발육과 afla toxin생산능력에 미치는 효과을 측정하기 위하여 강화배지에 $30^{\circ}C$ 9 일간 배양하였다. 백삼 saponin 첨가배지 만이 균체발율이 대조군 보다 좋았다. 그러나 홍삼 saponin 0.36% 첨가배지에서의 균체발육은 대조군의 62.3%로 나타났으나, aflatoxin 생산능력은 aflatoxin B, 이 대조군의 38.7%. aflatoxin G1 이 22.9%로 좋은 억제효과를 나타냈다. 우방자 sapollln은 홍삼 saponin보다 억세효과가 못하지만 홍삼 saponin과 같이 균체의 발육과 aflatoxin 생산을 모두 억제 하는 것으로 나타났다. 한편 extract 에서 나타났던 금은화의 aflatoxin 생산 억제효과는 본 saponin 실험에서는 나타나지 않았다. The research was carried out for the purpose of finding effects of gerbal saponins on aflatoxin synthesis by Aspergillus parasitics NRRL 2999. A. parasiticus with $10^6$ conidia were grown at $30^{\circ}C$ for 9 days on the enriched medium that is optimum for the frowth and aflatoxins production by the mold. The inhibitory effect on the growth and aflatoxins produced by the mold occurred in the presence of 0.36% of crude red-ginseng saponin showing both the growth and aflatoxins production come to 62.3% (growth), 38.7% (aflatoxin $B_1$) and 22.9% (aflatoxin $G_1$) of the control. Thd next effective saponin to inhibit the growth and aflatoxins production was from burdock seeds. However, saponin extracted from honeysuckle flowers had no inhibitory effect. The mold caused no changes in the pH of the medium when it contained red-ginseng saponin. Red-ginseng saponin was more effective than the white-ginseng in inhibiting both the growth and aflatoxin production.

나? 무서운 하지감자여!

박재림 샘터사 2016 월간 샘터 Vol.555 No.-

都市 市場地域 大氣中의 細菌에 對한 抗生物質 感受性 調査(釜山市)

박재림 新羅大學校 1977 論文集 Vol.5 No.-

Antibiotics susceptibilities were tested to get useful data for prevention food contamination and the treatment of infections such as septicemia. Tested were colony count, biochemical reaction for identification and resistant patterns to antimicrobial agents for 4 genus of bacteria isolated from the atmosphere of 5 markets in Busan on Aug. 1, 1976. The findings are as follows; 1. Out of the total colony count(1830), Pseudomonas was shown to be 55.5 per cent(1016). Staphylococcus 17 per cent(300), B. subtilis 1.9 per cent(35), Proteus spp. 0.1 per cent(3) and others 26 per cent(476). The area which showed the highest number of bacteria isolated was Bujeon market with 73.2 per cent while the lowest was Chungmoo market with 3.6 per cent and Oncheon, Bupyong, Changseon with 8.7 per cent, 8.6 per cent and 5.6 per cent, respectively. 37.3 per cent of colony was found in the morning(08:00), 33.7 per cent in the afternoon(16:00) and 29 per cent at noon. 2. Identified Staphlyococcus was resistant to antimicrobial agents except for chloramphenicol and sulfonamide, while Proteus spp. except for dihydrostreptomycin and tetracycline. Pseudomonas showed resistance to all agents, but B. subtilis showed susceptibilities except for tetracycline, penicillin and colistin.