Characteristics of Adherence and Invasion of Staphylococcus lugdunensis to Human Oral Epithelial Cells

Cha, J. D.,Lee, S. H.,Jung, K. Y.,Lim, D.S.,Woo, W. H.,Kim, K. J. Korean Academy of Oral Biology and the UCLA Dental 1999 International Journal of Oral Biology Vol.24 No.2

Staphulococcus lugdunensis is one of the most common pathogens in coagulase-negative staphylococci. Adhesion and invasion of S. lugdunensis to oral epithelial cells were demonstrated by recovery of viable organisms from gentamicin-treated KB cells, human oral epidemoid carcinoma cells. Adhesion was found to be time dependent and increased linearly with increasing number of bacteria added (10^3-10^5 CFU/well). Bacterial adhesion to the KB cells occurred via a cytochalasin B- and D-insensitive process. To clarify the time point and characteristics of S. lugdunensis invasion of KB cells, bacteria (10^4 CFU/well) were cocultered for several different times (1-5 hr) with or without cytochalasin B (1 ㎍/ml), follwed by additional coculture for 2hr in the presence of gentamicin (100㎍/ml). No bacteria were recovered at 2 hr, but a few colonies were detected after 3 hr of coculture. In addition, cytochalasin B also did not affect invasion of S. lugdunensis into KB cells. The data obtained here demonstrate that S. lugdunensis can adhere to the KB cells via a cytoskeletal actin protein-independent process; however its invasion through the cellular membrane is unclear. These data suggest a tissue-specific characteristic of pathogenesis for S. lugdunensis in in vivo.

인두편도염 환자와 보균자에서 분리한 Streptococcus pyogenes의 T-protein serotyping 및 항균제 감수성

이영희,황규잠,주영란,박강수,이광준,엄영길,배송미,차성호 국립보건연구원 1998 국립보건원보 Vol.35 No.-

Synthesis and characterization of macrocyclic nickel(II) complexes with α-methylbenzyl groups as chiral pendants

Han, J.H.,Cha, M.J.,Kim, B.G.,Kim, S.K.,Min, K.S. Elsevier 2008 Inorganic chemistry communications Vol.11 No.7

Novel nickel(II) hexaaza macrocyclic complexes, [Ni(L<SUP>R,R</SUP>)](ClO<SUB>4</SUB>)<SUB>2</SUB> (1) and [Ni(L<SUP>S,S</SUP>)](ClO<SUB>4</SUB>)<SUB>2</SUB> (2), containing chiral pendant groups have been synthesized by an efficient one-pot template condensation and characterized (L<SUP>R,R/S,S</SUP>=1,8-di((R/S)-α-methylbenzyl)-1,3,6,8,10,13-hexaazacyclotetradecane). The crystal structures of 1 and 2 were determined by single-crystal X-ray analysis. Each complex has a square-planar coordination environment for the nickel(II) ion, and is either an R or an S enantiomorph depending on the pendant groups. The circular dichroism spectrum of 1 showed a negative, positive and negative peak at 345, 440, and 492nm, respectively, and that of 2 exhibited an enantiomeric pattern.

여자 고등학생의 성태도와 부모 양육태도가 성폭력 인식수준에 미치는 영향

강수인,김수민,김유진,김지수,박지원,신수경,이지현,장은주,정채린,진애경,차지영,이수연 이화여자대학교 간호과학대학 2019 이화간호학회지 Vol.- No.53

Purpose: The study aimed to investigate the factors influencing consciousness of sexual violence in female high school students, focusing on sexual attitude and parental attitude. ethods: Data were collected from 248 female high school students in South Korea. A structured questionnaire was used for data collection through online survey. The collected data were analyzed by descriptive statistics, t-test, ANOVA, Tukey’s test, Pearson correlation coefficient, and multiple linear regression using the SPSS 25.0 program. Results: The mean score of consciousness of sexual violence in female high school students was 43.54 out of 48. The findings indicate that the mean score of consciousness of sexual violence differed y number of years in high school and academic achievement. Among sexual attitude, parental ttitude, and consciousness of sexual violence, there were meaningful correlations between onsciousness of sexual violence and sexual attitude (r=.132, p=.038,), love-hostility (r=.172, p=.006),and autonomous-control (r=.132, p=.038). Love-hostility aspect of parental attitude (=.159, p=.049) and academic achievement (=.208, p=.001) were found to be the important influencing factors on female high school student’s consciousness of sexual violence (F=5.720, p<.001). Conclusion: The findings of the study suggest that the love-hostility aspect of parental attitude and academic achievement influence consciousness of sexual violence of female high school students. Therefore, in order to improve consciousness of sexual violence in female high school students, sex education program and parent-education program should be developed with further research.

남자 대학생의 인터넷 음란물 노출 정도에 따른 성폭력 인지도와 성 태도

김정은,류지원,박보성,배수진,신서우,신유미,안세미,오혜수,정예지,조현아,최다해,차지영,박송이 이화여자대학교 간호학회 2018 이화간호학회지 Vol.- No.52

Purpose: The purpose of this study was to examine university male students’ exposure to internet pornography, and its influence on sexual violence awareness status and sexual attitude to provide educational materials. Methods: Data were collected through self-reported questionnaires from 286 male students who are attending universities in Seoul and Gyunggi-Do from August 20th to October 16th in 2017. 81 questionnaires in total were analyzed using mean, S.D, t-test, ANOVA, Scheffe with SPSS 22.0. Result: The results summarized according to research subject matter are as follows. First, most of the participants watched pornography 1-2 times per week(36.0%), 20mins-30mins(57.0%), heterosexual intercourse(92.0%) by cell phone(52.4%). Second, general characteristics of male university students’ influenced by sexual violence awareness status and sexual attitude showed significant difference in 4 factors of sexual attitude according to general characteristics of male university students in order of ‘chastity’, ‘homosexuality’, ‘contraception’, and ‘sexual consciousness’. Third, the influence of male university students’ internet pornography exposures on sexual awareness and sexual attitude showed significant difference in sexual awareness according to ‘change in sexual behavior after watching pornography’. There was significant difference in 3 factors of sexual attitude according to exposure of university male students to internet pornography. Conclusion: This study suggest that sexual education development for male university students is needed in order to establish upright sexual values that can lead to healthy sexual behaviors.

Biochemical analysis of recombinant CYP4A11 allelic variant enzymes: W126R, K276T and S353G

Han, S.,Cha, G.S.,Chun, Y.J.,Lee, C.H.,Kim, D.,Yun, C.H. JAPANESE SOCIETY FOR THE STUDY OF XENOBIOTICS 2016 DRUG METABOLISM AND PHARMACOKINETICS Vol.31 No.6

Human CYP4A11 is the major ω-hydroxylase of fatty acids in the liver and kidneys. It produces 20-hydroxyeicosatetraenoic acid as well as hydroxylates fatty acids. In this study, we investigated the biochemical properties of three alleles of CYP4A11: W126R, K276T, and S353G. Site-directed mutagenesis of the wild type CYP4A11 was performed, to construct the W126R, K276T, and S353G variant clones. The CYP4A11 wild type and variant constructs were heterologously expressed in Escherichia coli. CO-binding spectra showed the expression of the wild type, K276T and S353G variants, indicating the functional P450 holoenzyme. The W126R variant was not expressed in E. coli. Binding affinities of lauric acid in K276T and S353G variants were stronger than that of wild type. Steady-state kinetics in the hydroxylation reaction of fatty acids were studied. The catalytic efficiencies (k<SUB>cat</SUB>/K<SUB>m</SUB>) of K276T and S353G variants in the reactions without cytochrome b<SUB>5</SUB> were approximately 2- and 4-fold higher, respectively, than that of wild type, and in the reactions with cytochrome b<SUB>5</SUB> they were approximately 2- and 3-fold higher, respectively. These results suggest that individuals carrying the alleles, K276T and S353G, might exhibit higher catalysis of CYP4A11, which may affect the endogenous metabolic products associated with regulation of blood pressure.

Inflammatory lipid sphingosine-1-phosphate upregulates C-reactive protein via C/EBPβ and potentiates breast cancer progression

Kim, E-S,Cha, Y,Ham, M,Jung, J,Kim, S G,Hwang, S,Kleemann, R,Moon, A Macmillan Publishers Limited 2014 Oncogene Vol.33 No.27

A crucial role of the inflammatory lipid sphingosine-1-phosphate (S1P) in breast cancer aggressiveness has been reported. Recent clinical studies have suggested that C-reactive protein (CRP) has a role in breast cancer development. However, limited information is available on the molecular basis for the expression of CRP and its functional significance in breast cell invasion. The present study aimed to elucidate the molecular link between S1P and CRP during the invasive process of breast epithelial cells. This is the first report showing that transcription of CRP was markedly activated by S1P in breast cells. Our data suggest that not only S1P treatment but also the endogenously produced S1P may upregulate CRP in breast carcinoma cells. Transcription factors CCAAT/enhancer-binding protein beta and c-fos were required for S1P-induced CRP expression. Coupling of S1P<SUB>3</SUB> to heterotrimeric G<SUB>αq</SUB> triggered the expression of CRP, utilizing signaling pathways involving reactive oxygen species (ROS), Ca<SUP>2+</SUP> and extracellular signal-related kinases (ERKs). S1P-induced CRP expression was crucial for the transcriptional activation of matrix metalloproteinase-9 through ERKs, ROS and c-fos, leading to breast cell invasion. Using a xenograft mice tumor model, we demonstrated that S1P induced CRP expression both in vitro and in vivo. Taken together, our findings have revealed a molecular basis for S1P-induced transcriptional activation of CRP and its functional significance in the acquisition of the invasive phenotype of human breast epithelial cells under inflammatory conditions. Our findings may provide useful information on the identification of useful therapeutic targets for inflammatory breast cancer.

Antibiotic susceptibility and resistance of Streptococcus iniae and Streptococcus parauberis isolated from olive flounder (Paralichthys olivaceus)

Park, Y.K.,Nho, S.W.,Shin, G.W.,Park, S.B.,Jang, H.B.,Cha, I.S.,Ha, M.A.,Kim, Y.R.,Dalvi, R.S.,Kang, B.J.,Jung, T.S. Elsevier Scientific Pub. Co 2009 Veterinary microbiology Vol.136 No.1

The rates of antibiotic susceptibility and resistance were investigated in Streptococcus iniae and Streptococcus parauberis isolates obtained from diseased olive flounders (Paralichthys olivaceus) collected from fish farms in Jeju Island, Korea. Isolates of S. iniae (n=65) were susceptible to cefotaxime, erythromycin, ofloxacin, penicillin, tetracycline and vancomycin, as demonstrated by the minimum inhibitory concentration (MIC) test. Isolates of S. parauberis (n=86) were highly resistant to erythromycin (58% of the 86 isolates tested) and tetracycline (63% of the 86 isolates tested). Fifty-four isolates of tetracycline-resistant S. parauberis contained the tet(M/O/S) genes, of which 39 and 12 isolates contained the tet(M) and tet(S) genes, respectively, whereas 3 isolates contained both the tet(M) and tet(S) genes. Among the erythromycin-resistant isolates of S. parauberis (n=50) only 14 contained the erm(B) gene. These results suggest that the tet(S) and erm(B) genes of S. parauberis are involved in the acquisition of high-level resistance to erythromycin and tetracycline. Our findings reveal a high rate of antibiotic resistance among strains of S. parauberis and emphasize the need to develop an appropriate vaccine to reduce the use of antibiotics.

Electrochemical properties of gel copolymer- electrolyte based on Phosphonium ionic liquid

Cha, E.H.,Lim, S.A.,Park, J.H.,Kim, D.W.,Park, J.H. The Korean Electrochemical Society 2008 한국전기화학회지 Vol.11 No.4

Noble Poly (lithium 2-acrylamido-2-methyl propane sulfonate) and its copolymer with N-vinyl formamide based on trihexyl (tetradecyl) phosphonium acetate [$(C_6H_{13})_3$ P ($C_{14}H_{29}$) $CH_3COO$; $P_{66614}$ $CH_3COO$] and trihexyl (tetradecyl)phosphonium bis(trifluoromethane sulfonyl) amide ([$(C_6H_{13})_3P(C_{14}H_{29})$] [TFSA];$P_{66614}TFSA$) were prepared and analyzed to determine their characteristics and properties. The ionic conductivity of a copolymer based $P_{66614}TFSA$ ionic liquid system exhibits a higher conductivity ($8.9{\times}10^{-5}Scm^{-1}$) than that of a copolymer based $P_{66614}CH_3COO$ system ($1.57{\times}10^{-5}Scm^{-1})$. The charge on the TFSA anion is spread very diffusely through the S-N-S core and particularly in the trifluoromethane groups, and this diffusion results in a decreased interaction between the cation and the anion. The viscosity of $P_{66614}TFSA$ (39 cP at 343 K) and $P_{66614}CH_3COO$ (124 cP at 343 K), which is very hydrophobic, was fairly high. High viscosity leads to a slow rate of diffusion of redox species. The ionic conductivity of copolymer of a phosphonium ionic liquid system also exhibits higher conductivity than that of a homopolymer system. Phosphonium ionic liquids were thermally stable at temperatures up to $400^{\circ}C$.

Ritter's disease의 2 례

변희탁,차정인,이보근,이효권,이정일,설수길 대한소아과학회 1971 小兒科 Vol.14 No.12