Novel anti-apoptotic mechanism of A20 through targeting ASK1 to suppress TNF-induced JNK activation

Won, M,Park, K A,Byun, H S,Sohn, K-C,Kim, Y-R,Jeon, J,Hong, J H,Park, J,Seok, J H,Kim, J M,Yoon, W-H,Jang, I-S,Shen, H M,Liu, Z G,Hur, G M Macmillan Publishers Limited 2010 CELL DEATH AND DIFFERENTIATION Vol.17 No.12

The zinc-finger protein A20 has crucial physiological functions as a dual inhibitor of nuclear factor-κB (NF-κB) activation and apoptosis in tumor necrosis factor (TNF) receptor 1 signaling pathway. Although the molecular basis for the anti-NF-κB function of A20 has been well elucidated, the anti-apoptotic function of A20 is largely unknown. Here, we report a novel mechanism underlying the anti-apoptotic function of A20: A20 blocks TNF-induced apoptosis through suppression of c-jun N-terminal kinase (JNK) by targeting apoptosis signal-regulating kinase1 (ASK1). First, the ectopic expression of A20 drastically inhibits TNF-induced JNK activation and apoptosis in multiple cell types including those deficient of NF-κB activation. Unexpectedly, the blunting effect of A20 on TNF-induced JNK activation is not mediated by affecting the TNFR1 signaling complex formation. Instead, A20 interacts with ASK1, an important MAPKK kinase in the JNK signaling cascade. More importantly, overexpression of wild-type A20, but not of mutant A20 (ZnF4; C624A, C627A), promotes degradation of the ASK1 through the ubiquitin-proteasome system. Taken together, the results from this study reveal a novel anti-apoptotic mechanism of A20 in TNF signaling pathway: A20 binds to ASK1 and mediates ASK1 degradation, leading to suppression of JNK activation and eventually blockage of apoptosis.

V994 Herculis: the multiple system with a quadruple-lined spectrum and a double eclipsing feature

Lee, C.-U.,Kim, S.-L.,Lee, J. W.,Kim, C.-H.,Jeon, Y.-B.,Kim, H.-I.,Yoon, J.-N.,Humphrey, A. Blackwell Publishing Ltd 2008 MONTHLY NOTICES- ROYAL ASTRONOMICAL SOCIETY Vol.389 No.4

<P>ABSTRACT</P><P>We report the discovery of a multiple system with a quadruple-lined spectrum and a double eclipsing feature. Our photometric and high-resolution spectroscopic data show that V994 Herculis (V994 Her; ADS 11373 AB) is composed of two pairs of double-lined eclipsing binaries, which we designate as A and B. System A consists of a B8V+A0V binary with an orbital period of 2.083 264 d and system B of a A2V+A4V binary with 1.420 033 d. Our light curves show that both of them have a detached binary configuration. We derive masses and radii of four components (Aa, Ab, Ba and Bb) from the synthetic analyses of light curves and radial velocity curves. The masses of systems A and B are <I>M</I><SUB>Aa</SUB>= 2.83 ± 0.20 M<SUB>⊙</SUB>, <I>M</I><SUB>Ab</SUB>= 2.30 ± 0.16 M<SUB>⊙</SUB>, <I>M</I><SUB>Ba</SUB>= 1.87 ± 0.12 M<SUB>⊙</SUB> and <I>M</I><SUB>Bb</SUB>= 1.86 ± 0.12 M<SUB>⊙</SUB>, with radii <I>R</I><SUB>Aa</SUB>= 2.15 ± 0.05 R<SUB>⊙</SUB>, <I>R</I><SUB>Ab</SUB>= 1.71 ± 0.04 R<SUB>⊙</SUB>, <I>R</I><SUB>Ba</SUB>= 1.59 ± 0.08 R<SUB>⊙</SUB> and <I>R</I><SUB>Bb</SUB>= 1.50 ± 0.08 R<SUB>⊙</SUB>, respectively. These masses and radii are well consistent with the empirical relation for double-lined eclipsing binaries.</P>

Satisfaction with mammography in the National Cancer Screening Programme participants of age 40s in Korea

JEON, B.Y.,LEE, H‐,Y.,PARK, E‐,C.,CHOI, K.S.,JUN, J.K.,KIM, Y.,HAN, M.A.,YOON, N‐,H.,KIM, E.J.,JEON, S.M. Blackwell Publishing Ltd 2011 European journal of cancer care Vol.20 No.6

<P>JEON B.Y., LEE H‐Y., PARK E‐C., CHOI K.S., JUN J.K., KIM Y., HAN M.A., YOON N‐H., KIM E.J. & JEON S.M. (2011) <I>European Journal of Cancer Care</I><B>20</B>, 803–809</P><P><B>Satisfaction with mammography in the National Cancer Screening Programme participants of age 40s in Korea</B></P><P>The aim of this study was to evaluate satisfaction with the National Cancer Screening Programme of mammography in Korea and to examine the association between subscales of satisfaction and general satisfaction. We conducted a cross‐sectional telephone survey for women who had obtained a National Cancer Screening Programme mammographic screening at general hospitals between May and October 2008. The present study included 2005 women in their forties. We performed multivariate linear regression using dependent variable as general satisfaction and independent variables as subscales of satisfaction, such as pre‐screening information transfer, staff interpersonal skills, physical surroundings and results reporting. Participants were stratified according to the result of their mammogram as negative or positive. Mean score of satisfaction was above 2.5 of 4 for all subscales. Women who received positive results were less satisfied with all of subscale factors. Staff interpersonal skills were the most important factor that contributed to general satisfaction. Future efforts such as staff training programme of communication/attitude skills, ensuring privacy and explanation of possible discomfort of the screening would be needed.</P>

여대생의 월경불편감 정도와 식습관에 관한 연구

김선영,김준희,김찬영,박정우,신선미,안선영,이민혜,이정아,임미선,전현정 이화여자대학교 간호과학대학 2010 이화간호학회지 Vol.- No.44

Purpose: This study was done to identify the correlation between the menstrual discomforts and dietary habits of young university women in order to provide the basic needs and make improvements on the quality of life for women. Method: The Menstrual Discomfort Questionnaire (MDQ) and Nutrition Diagnosis for Health were the tools used. The participants were 279 female university students in Seoul. Result: Factors related to MDQ were found to be the VAS scores (r=.361, p=.002), menstrual discomfort according to stress consciousness (F=3.756, p=.025), experiences of taking medicine (F= 4.130 p= .043), the types of residential forms (F=6.197, p=.000), recognition of menstrual period (F=5.466, p=.020), eating breakfast, the frequency of carbohydrates intake (F=5.28, p=.006), the frequency of vegetable intake, seaweed and mushrooms (F=4.98, p=.008). As a result of the study, there is a correlation between menstrual discomforts of female university students and dietary habits. Conclusion: In order to decrease the discomforts of the menstrual cycle that usually includes pain and bloating a well-balanced dietary habit is needed. A balance diet includes grain, vegetables, and mushroom should be taken in normal meals. We should confine the dietary habits that form severe menstrual discomfort in people and give them recommendations for ideal dietary habits that would ideally decrease the pain caused by menstruation. Therefore it is necessary that the education program and nursing mediation should be developed as a program that would aid in decreasing menstrual discomforts. In future studies, a similar study with at a larger scale should be conducted with more recommended specified nutritional assessment.

High galectin-1 expression correlates with poor prognosis and is involved in epithelial ovarian cancer proliferation and invasion

Kim, H.J.,Jeon, H.K.,Cho, Y.J.,Park, Y.A.,Choi, J.J.,Do, I.G.,Song, S.Y.,Lee, Y.Y.,Choi, C.H.,Kim, T.J.,Bae, D.S.,Lee, J.W.,Kim, B.G. Pergamon Press 2012 European journal of cancer Vol.48 No.12

Purpose: Galectin-1 (Gal-1) is a 14-kDa laminin-binding galectin involved in several biological events including regulation of tumour proliferation and metastasis. In this study, we investigated the clinical significance of Gal-1 expression and its functional role in cell proliferation and invasion in epithelial ovarian cancer (EOC). Experimental design: We evaluated the expression of Gal-1 in 52 serous, 11 endometrioid, and 3 mucinous type EOC tumour samples from 66 patients by immunohistochemistry. In vitro experiments were performed to determine the function of Gal-1 in cell survival, proliferation, and invasion in EOC cells using siRNA and anginex, a Gal-1 inhibitor, as well as recombinant Gal-1 protein. Results: Patients with strong Gal-1 peritumoural staining had poorer progression-free survival (PFS) than patients with weak peritumoural staining (p=0.03). Inhibition of Gal-1 by siRNA or anginex resulted in the inhibition of cell growth and proliferation of HeyA8 and SKOV3ip1 cells. Moreover, the ability of cells to migrate was significantly reduced by treatment of cells with Gal-1 siRNA but was increased by treatment of cells with recombinant Gal-1. When we evaluated the interaction between fibroblasts (T HESCs) and cancer cells (A2780-CP20), we found that MMP-2 expression in cancer cells was affected by Gal-1 secreted by fibroblast cells, which suggests that Gal-1 in human fibroblasts might affect the invasive abilities of tumour cells. Conclusion: Our results suggest that Gal-1 expression is a potential prognostic factor for PFS and that Gal-1 could be a novel treatment target in EOC patients.

Beijing urban particulate matter-induced injury and inflammation in human lung epithelial cells and the protective effects of fucosterol from <i>Sargassum binderi</i> (Sonder ex J. Agardh)

Fernando, I.P. Shanura,Jayawardena, Thilina U.,Kim, Hyun-Soo,Lee, Won Woo,Vaas, A.P.J.P.,De Silva, H.I.C.,Abayaweera, G.S.,Nanayakkara, C.M.,Abeytunga, D.T.U.,Lee, Dae-Sung,Jeon, You-Jin Academic Press 2019 Environmental research Vol.172 No.-

<P><B>Abstract</B></P> <P>Particulate matter (PM) air pollution has gradually become a widespread problem in East Asia. PM may cause unfamiliar inflammatory responses, oxidative stress, and pulmonary tissue damage, and a comprehensive understanding of the underlying mechanisms is required in order to develop effective anti-inflammatory agents. In this study, fine dust collected from Beijing, China (CPM) (size < PM13 with majority < PM2.5) was evaluated for its oxidative stress- and inflammation-inducing effects, which cause cell damage, in A459 human lung epithelial cells. Oxidative stress was marked by an increase in intracellular ROS levels and the production of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), and heme oxygenase-1 (HO-1). Upon induction of oxidative stress, a marked increase was observed in the expression of key inflammatory mediators such as COX-2 and PGE<SUB>2</SUB> and the pro-inflammatory cytokines TNF-α and IL-6 via NF-kB and MAPK pathways. Cellular damage was marked by a reduction in viability, increased lactate dehydrogenase (LDH) release, formation of apoptotic and necrotic bodies, accumulation of sub-G1 phase cells, and DNA damage. Apoptosis was found to be mediated via the activation of caspases through the mitochondria-mediated pathway. Fucosterol, purified from the brown alga <I>Sargassum binderi</I> (Sonder ex J. Agardh) by bio-assay-guided fractionation and purification, exhibited potential therapeutic effects against CPM-induced detrimental effects. Further studies could focus on developing fucosterol, in forms such as steroidal inhalers, against PM-induced pulmonary tissue inflammation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Fine dust air pollution is a major reason of pulmonary complications in East Asia. </LI> <LI> Dust particles induce oxidative stress and inflammation damaging the lung epithelial cells. </LI> <LI> Fucosterol suppressed the dust induced cell damage by inhibiting oxidative stress and inflammation. </LI> <LI> Fucosterol may have beneficial effects in alleviating adverse respiratory effects of air pollution. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Effects of excess Bi₂O₃ on grain orientation and electrical properties of CaBi₄Ti₄O₁₅ ceramics

Cho, S.Y.,Choi, G.P.,Jeon, D.H.,Johnson, T.A.,Lee, M.K.,Lee, G.J.,Bu, S.D. Elsevier 2015 CURRENT APPLIED PHYSICS Vol.15 No.11

A CaBi<SUB>4</SUB>Ti<SUB>4</SUB>O<SUB>15</SUB> (CBTO) ceramic in which the Bi<SUB>2</SUB>O<SUB>3</SUB> concentration was controlled from 0 to 10 wt% was fabricated using a solid-state reaction method. Structural analysis by field-emission scanning electron microscopy (FE-SEM) and X-ray diffraction (XRD) indicated differences in the preferred grain orientation and size of the plate-like grains according to the Bi<SUB>2</SUB>O<SUB>3</SUB> concentration. The orientation of plate-like grains was also found to vary with the Bi<SUB>2</SUB>O<SUB>3</SUB> concentration. There was no noticeable change trend of dielectric properties with different Bi<SUB>2</SUB>O<SUB>3</SUB> concentrations. Relatively low dielectric constants (about 135) were exhibited by the CBTO ceramic with 1 wt% Bi<SUB>2</SUB>O<SUB>3</SUB> and CBTO ceramic with 10 wt% Bi<SUB>2</SUB>O<SUB>3</SUB> only, and similar values (about 150) were exhibited by the other ceramics. The dielectric loss exhibited a low value in the range of 0.01-0.09 for all samples (frequency range of 1-100 kHz). Regarding the ratio changes of the piezoelectric coefficient (d<SUB>33</SUB>) and the ratio of a-axis orientation of plate-like grains, the trends of these two values were shown to be similar. These results suggest that the addition of Bi<SUB>2</SUB>O<SUB>3</SUB> greatly influences the microstructure of CBTO ceramics, including the grain size and orientation of plate-like grains. In particular, the change in the preferred grain orientation is closely related to the change in the piezoelectric properties.

중년남성의 지각된 건강상태, 가족응집력 및 건강증진행위에 관한 연구

김지선,노원영,박새별,유동민,이가은,이주애,전유라,조세영,차지영,고지현 이화여자대학교 간호과학대학 2016 이화간호학회지 Vol.- No.50

health promotion behavior of middle aged men and the relationship among the above factors. Methods: Subjects of the research were 325 middle aged men in Korea. The questionnaires were composed of general characteristics, Health Self Rating Scale, FACES-Ⅲ : Family Adaptability & Cohesion Evaluation Scale Ⅲ, Health Promoting Lifestyle Profile. Data were statistically analyzed by t-test, ANOVA, Scheffe test and Pearson’s correlation. Results: Middle aged men whose perceived economic status are low show lower perceived health status than moderate and high (F=3.364, p=.010). Health promotion behavior in the age 40-44 shows lower level than 60-64 (F=2.984, p=.019). Middle aged men who have Economically dependent adult in the first child show higher level in health promotion behavior than who have Middle & High school student in the first child (F=2.468, p=.045). In the middle aged men, perceived health status and family cohesion show positive correlation (r=.341, p<.01). Perceived health status and health promotion behavior show positive correlation (r=.500, p<.01). Family cohesion and health promotion behavior show posotive correlation (r=.564, p<.01). Conclusions: We analyzed influencing factor and a relationship between perceived health status, family cohesion and health promotion behavior. Accordingly, we can think of applying nursing interventions to the middle aged men considering the general characteristics. Also we can think of applying nursing interventions and educations to help them evaluate their perceived health status and include the support of family members for the health promotion behavior.

High antiangiogenic and low anticoagulant efficacy of orally active low molecular weight heparin derivatives

Park, J.W.,Jeon, O.C.,Kim, S.K.,Al-Hilal, T.A.,Jin, S.J.,Moon, H.T.,Yang, V.C.,Kim, S.Y.,Byun, Y. Elsevier Science Publishers 2010 Journal of controlled release Vol.148 No.3

Heparin, an anticoagulant that is widely used clinically, is also known to bind to several kinds of proteins through electrostatic interactions because of its polyanionic character. These interactions are mediated by the physicochemical properties of heparin such as sequence composition, sulfation patterns, charge distribution, overall charge density, and molecular size. Although this electrostatic character mediates its binding to many proteins related with tumor progression, thereby providing its antiangiogenic property, the administration of heparin for treating cancer is limited in clinical applications due to several drawbacks, such as its low oral absorption, unsatisfactory therapeutic effects, and strong anticoagulant activity which induces hemorrhaging. Here, we evaluated novel, orally active, low molecular weight heparin (LMWH) derivatives (LHD) conjugated with deoxycholic acid (DOCA) that show reduced anticoagulant activity and enhanced antiangiogenic activity. The chemical conjugate of LMWH and DOCA was synthesized by conjugating the amine group of N-deoxycholylethylamine (EtDOCA) with the carboxylic groups of heparin at various DOCA conjugation ratios. The LMWH-DOCA conjugate series (LHD1, LHD1.5, LHD2, and LHD4) were further formulated with poloxamer 407 as a solubilizer for oral administration. An in vitro endothelial tubular formation and in vivo Matrigel plug assay were performed to verify the antiangiogenic potential of LHD. Finally, we evaluated tumor growth inhibition of oral LHD administration in a SCC7 model as well as in A549 human cancer cell lines in a mouse xenograft model. Increasing DOCA conjugation ratios showed decreased anticoagulant activity, eventually to zero. LHD could block angiogenesis in the tubular formation assay and the Matrigel plug assay. In particular, oral administration of LHD4, which has 4 molecules of DOCA per mole of LMWH, inhibited tumor growth in SCC7 mice model as well as A549 mice xenograft model. LHD4 was orally absorbable, showed minimal anticoagulant activity and inhibits tumor growth via antiangiogenesis. These findings demonstrate the therapeutic potential of LHD4 as a new oral anti-cancer drug.

Longer-range lattice anisotropy strongly competing with spin-orbit interactions in pyrochlore iridates

Hozoi, L.,Gretarsson, H.,Clancy, J. P.,Jeon, B.-G.,Lee, B.,Kim, K. H.,Yushankhai, V.,Fulde, Peter,Casa, D.,Gog, T.,Kim, Jungho,Said, A. H.,Upton, M. H.,Kim, Young-June,van den Brink, Jeroen American Physical Society 2014 Physical review. B, Condensed matter and materials Vol.89 No.11

In the search for topological phases in correlated electron systems, materials with 5d transition-metal ions, in particular the iridium-based pyrochlores A2Ir2O7, provide fertile grounds. Several topological states have been predicted but the actual realization of such states is believed to critically depend on the strength of local potentials arising from distortions of the IrO6 cages. We test this hypothesis by measuring with resonant inelastic x-ray scattering the electronic level splittings in the A = Y, Eu systems, which we show to agree very well with ab initio quantum chemistry electronic-structure calculations for the series of materials with A = Sm, Eu, Lu, and Y. We find, however, that the primary source for quenching the spin-orbit interaction is not a distortion of the IrO6 octahedra but longer-range lattice anisotropies which inevitably break the local cubic symmetry.