Variant Allele Frequency of Pseudogene-Related Variants in Short-read Next-Generation Sequencing Data May Mislead Genetic Diagnosis: A Case of Shwachman-Diamond Syndrome

Lee Heerah,Lee Jung Ae,Lee Hyesu,Lee Jee Soo,Ko Jung Min,Kim Man Jin,Seong Moon-Woo 대한진단검사의학회 2023 Annals of Laboratory Medicine Vol.43 No.6

Assuring explainability on demand response targeting via credit scoring

Lee, Kyungeun,Lee, Hyesu,Lee, Hyoseop,Yoon, Yoonjin,Lee, Eunjung,Rhee, Wonjong Elsevier 2018 ENERGY Vol.161 No.-

<P><B>Abstract</B></P> <P>As data-driven innovation becomes a main trend in the energy sector, explainability of data-driven actions is becoming a major fairness issue for the residential applications, and it is expected to become a requirement for regulatory compliance. Explainability, however, often demands a sacrifice in prediction performance and affects the effectiveness of data-driven actions. In this study, we consider data-driven customer targeting in an incentive-based residential demand response program, and investigate the explainability-performance tradeoff when using simple-rule based, machine learning, and credit scoring methods. Credit scoring, that has been a popular solution in the finance discipline for over 60 years, is a scorecard based modeling method that can surely provide explainability. We first provide the detailed steps of applying credit scoring to the demand response problem. Then, we use a dataset of 14,525 households obtained from a real demand response program and analyze two prediction problems – participation prediction and behavior change prediction. The results show that credit scoring can achieve a comparable performance as the best-performing machine learning methods while providing full explainability. Our results suggest that credit scoring can be a promising explainability option for broader energy sector problems.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A quantitative analysis of data-driven targeting in residential DR. </LI> <LI> Explainability of data-driven actions and its relation to fairness. </LI> <LI> Details of implementing credit scoring, which has good explainability, for DR. </LI> <LI> A case study of incentive DR, where the DR was operated through a smartphone app. </LI> <LI> Credit scoring can achieve a comparable performance as machine learning methods. </LI> </UL> </P>

Copper(I)-Catalyzed Enantioselective 1,6-Borylation of α,β,γ,δ-Unsaturated Phosphonates

Lee, Hyesu,Yun, Jaesook American Chemical Society 2018 ORGANIC LETTERS Vol.20 No.24

<P>Copper(I)-catalyzed asymmetric 1,6-borylation of 1,3-dienylphosphonates was achieved using (<I>S</I>,<I>S</I>)-Ph-BPE as a chiral ligand. Regio-, stereo-, and enantioselective borylation successfully proceeded to afford phosphonate-containing allylboronates, with high enantioselectivity up to 97% ee. Further applications of the resulting products generated a valuable phosphonate analogue of γ-butyrolactone.</P> [FIG OMISSION]</BR>

Dietary Assessment of Korean Non-dialysis Chronic Kidney Disease Patients with or without Diabetes

Hyesu Lee,김현숙,Tae Yeon Kim,류현진,주달래,Miyoung Jang,오국환,Curie Ahn,한성림 대한의학회 2020 Journal of Korean medical science Vol.35 No.23

Background: Dietary intervention at the early stage of chronic kidney disease (CKD) is important for preventing progression to the end-stage renal disease (ESRD). However, few studies have investigated dietary intake of CKD patients in non-dialysis stage. Therefore, we investigated the dietary intake of Korean non-dialysis CKD patients and aimed to establish baseline data for the development of dietary education and intervention strategies for CKD patients. Methods: Three hundred fifty CKD patients who visited Seoul National University Hospital outpatient clinic from February 2016 to January 2017 were recruited for this cross-sectional study. Subjects on dialysis and those who had undergone kidney transplantation were excluded. Dietary intake, demographic information, and biochemical characteristics of 256 subjects who completed three-day dietary records were analyzed. Subjects were divided into four groups based on diabetes mellitus (DM) (DM-CKD and Non-DM-CKD groups) and kidney function (Early-CKD and Late-CKD groups). Results: Total energy intake was lower in the Late-CKD group compared with the Early-CKD group. In men, carbohydrate intake was higher and protein and fat intakes tended to be lower in the Late-CKD group compared with the Early-CKD group. In women, carbohydrate intake tended to be lower in the DM-CKD group than the Non-DM-CKD group. Protein intake tended to be higher in the DM-CKD groups. Phosphorus and sodium intakes were higher in the DM-CKD groups compared with the Non-DM-CKD groups in women, and tended to be higher in the DM-CKD groups in men. Conclusion: DM and kidney function affected energy and nutrient intakes. Subjects in the Late-CKD group consumed less energy than those in the Early-CKD group. Non-DM subjects seemed to restrict protein intake starting from the Early-CKD stage than subjects with DM. Subjects in this study had low energy and high sodium intakes compared with recommended levels. Protein intake was lower in advanced CKD patients, but their intake level was still higher than the recommendation. Dietary intervention strategies for non-dialysis CKD patients need to be customized depending on the presence of DM and kidney function.

Copper(I)–Taniaphos Catalyzed Enantiodivergent Hydroboration of Bicyclic Alkenes

Lee, Hyesu,Lee, Byoung Yoon,Yun, Jaesook American Chemical Society 2015 ORGANIC LETTERS Vol.17 No.3

<P>In this study, highly enantioselective copper(I)-catalyzed hydroboration of bicyclic alkenes is reported. Using a copper-taniaphos complex, excellent enantioselectivities up to >99% ee were obtained for bicyclic alkenes including oxa- and azabicyclic alkenes. Furthermore, copper-catalyzed enantiodivergent hydroboration methods with the same chiral ligand-copper precursors were developed using different boron sources based on alternative mechanistic pathways.</P>

Pyrazolo[3,4‐ d ]pyrimidine derivatives as irreversible Bruton's tyrosine kinase inhibitors

Yeom Hyesu,Achary Raghavendra,Choi Yunha,Park Chi Hoon,Lee Joo‐Youn,Lee Heung Kyoung,Kim Pilho,Cho Sung Yun 대한화학회 2022 Bulletin of the Korean Chemical Society Vol.43 No.4

4,6-Disubstituted pyrazolo[3,4-d]pyrimidine derivatives were explored as irreversible Bruton’s tyrosine kinase (BTK) inhibitors. The structure–activity relationship was established with over 20 derivatives synthesized to determine initial hit compounds, based on activities against BTK enzyme and TMD8 cells. It turned out that introducing 1-acrylamido-4-aminopiperdine (1b) at the C4 position of pyrazolopyrimidine as in 5e, and 3-acrylamido-aniline (1j) as 4-position substituent, such as in 9d, 10d, and 10e, delivered potent in vitro enzyme activities as well as TMD8 cell-based cytotoxicities. Considering kinase selectivity profiles, 5e was selected for in vivo efficacy studies with a murine xenograft model using TMD8 cells, where 5e exhibited moderate tumor growth inhibition activities. Further optimization of 5e and 9d may lead to clinically useful compounds to overcome B-cell-mediated hematologic cancers.

LC-MS/MS를 이용한 벌꿀 중 잔류동물용의약품 분석법 개발

이혜수 ( Hyesu Lee ),심진하 ( Jin Ha Sim ),김지영 ( Ji Young Kim ),신동우 ( Dong-woo Shin ),김현경 ( Hyun-kyung Kim ) 한국환경농학회 2023 한국환경농학회 학술대회집 Vol.2023 No.0

The Positive List System (PLS) will be implemented for veterinary drug residues in food from 2024, therefore this study focused on the establishment of more sensitive analytical method for veterinary drug residues in honey, propolis, and royal jelly. For analysis, 8 veterinary drugs frequently used in honey, such as antibiotics and anthelmintics, were selected. The target analytes were extracted from honey, propolis, and royal jelly samples by using 80% acetonitrile, and then purified with C₁₈ and acetonitrile saturated with n-hexane. The LC-MS/MS analysis was performed on a reverse-phase C₁₈ column (2.1 × 150 mm, 3.5 μm) with gradient elution using 0.1% formic acid in water and 0.1% formic acid in acetonitrile as mobile phases. Method validation was proceeded for honey, propolis, and royal jelly at three concentrations, 1×LOQ, 2×LOQ and 10×LOQ (limit of quantification) or 0.5×MRL, 1×MRL and 2×MRL. The coefficient of linearity presents good linearity at matrix-matched calibration curves (R²>0.99). At all concentrations, the recoveries were ranged from 70.9% to 118.9% and the coefficient of variability (CV) were lower than 10%. These results met the CODEX guideline (CAC/GL 71-2009) requirements to analyze veterinary drug residues in food products.

浦項 및 장기盆地에 대한 古地磁氣, 層序 및 構造硏究

Hyun Koo Lee(李鉉具),Hi Soo Moon(文熙壽),Kyung Duck Min(閔庚德),ln Soo Kim(金仁洙),Hyesu Yun(尹惠洙),Tetsumaru Itaya(板谷徹丸) 대한자원환경지질학회 1992 자원환경지질 Vol.25 No.3

The Tertiary basins in Korea have widely been studied by numerous researchers producing individual results in sedimentology, paleontology, stratigraphy, volcanic petrology and structural geology, but interdisciplinary studies, inter-basin analysis and basin-forming process have not been carried out yet. Major work of this study is to elucidate evidences obtained from different parts of a basin as well as different Tertiary basins (Pohang, Changgi, Eoil, Haseo and Ulsan basins) in order to build up the correlation between the basins, and an overall picture of the basin architecture and evolution in Korea. According to the paleontologic evidences the geologic age of the Pohang marine basin is dated to be late Lower Miocence to Middle Miocene, whereas other non-marine basins are older as being either Early Miocene or Oligocene(Lee, 1975, 1978: Bong, 1984: Chun, 1982: Choi et al., 1984: Yun et al., 1990: Yoon, 1982). However, detailed ages of the Tertiary sediments, and their correlations in a basin and between basins are still controversial, since the basins are separated from each other, sedimentary sequence is disturbed and intruded by voncanic rocks, and non-marine sediments are not fossiliferous to be correlated. Therefore, in this work radiometric, magnetostratigraphic, and biostratigraphic data was integrated for the refinement of chronostratigraphy and synopsis of stratigraphy of Tertiary basins of Korea. A total of 21 samples including 10 basaltic, 2 porphyritic, and 9 andesitic rocks from 4 basins were collected for the K-Ar dating of whole rock method. The obtained age can be grouped as follows: 14.8±0.4~15.2±0.4 Ma, 19.9±0.5~22.1±0.7 Ma, 18.0±1.1~20.4±0.5 Ma, and 14.6±0.7~21.1±0.5 Ma. Stratigraphically they mostly fall into the range of Lower Miocene to Mid Miocene. The oldest volcanic rock recorded is a basalt (911213-6) with the age of 22.05±0.67 Ma near Sangjeong-ri in the Changgi (or Janggi) basin and presumed to be formed in the Early Miocene, when Changgi Conglomerate began to deposit. The youngest one (911214-9) is a basalt of 14.64±0.66 Ma in the Haseo basin. This means the intrusive and extrusive rocks are not a product of sudden voncanic activity of short duration as previously accepted but of successive processes lasting relatively long period of 8 or 9 Ma. The radiometric age of the volcanic rocks is not randomly distributed but varies systematically with basins and localities. It becomes generlly younger to the south, namely from the Changgi basin to the Haseo basin. The rocks in the Changgi basin are dated to be from 19.92±0.47 to 22.05±0.67 Ma. With exception of only one locality in the Geumgwangdong they all formed before 20 Ma B.P. The Eoil basalt by Tateiwa in the Eoil basin are dated to be from 20.44±0.47 to 18.35±0.62 Ma and they are younger than those in the Changgi basin by 2~4 Ma. Specifically, basaltic rocks in the sedimentary and voncanic sequences of the Eoil basin can be well compared to the sequence of associated sedimentary rocks. Generally they become younger to the stratigraphically upper part. Among the basin, the Haseo basin is characterized by the youngest volcanic rocks. The basalt (911214-7) which crops out in Jeongja-ri, Gangdong-myon, Ulsan-gun is 16.22±0.75 Ma and the other one (911214-9) in coastal area, Jujon-dong, Ulsan is 14.64±0.66 Ma old. The radiometric data are positively collaborated with the results of paleomagnetic study, pull-apart basin model and East Sea spreading theory. Especially, the successively changing age of Eoil basalts are in accordance with successively changing degree of rotation. In detail, following results are discussed. Firstly, the porphyritic rocks previously known as Cretaceous basement (911213-2, 911214-1) show the age of 43.73±1.05 및 49.58±1.13 Ma(Eocene) confirms the results of Jin et al. (1988). This means sequential volcanic activity from Cretaceous up to Lower Tertiary. Secondly, intrusive andesitic rocks

Method Development of Wood Volatile Organic Compounds Analysis using HS-INME Coated with GO;PANI followed by GC/MS

Sehyun Kim,Jinyoung Park,Daeun Lee,Hyesu Lee,Sunyoung Bae,Dongsun Lee 한국분석과학회 2021 학술대회논문집 Vol.2021 No.11

Volatile organic compounds (VOCs) released by plants are the main metabolites. Plant VOCs are distributed by almost all types of plants and are released either consitutively or in response to a variety of stimuli. Plant VOCs have a variety of roles, such as indirect plant defense against insects, attraction to pollinators, communication between plants, adaptation to heat resistance and environmental stress, and defense from predators. In addition, the emission of plant VOCs are highly species dependent. In this study, the VOCs from Juniperus virginia, Juniperus chinensis, Cinnamomum camphora, and Santalum album were analyzed using headspace-in-needle microextraction (HS-INME) method with graphene oxide;polyaniline (GO;PANI) adsorbent followed by GC/MS. Cyclic voltammetry (CV) method was applied to fabricate GO;PANI adsorbent on stainless steel wire. α-Longipinene, α-cedrene, and cedrol corresponding to the typical scent of wood were chosen as target materials and used for optimization processes. Adsorbent production and HS-INME extraction conditions were optimized through the response surface methodology (RSM) to which the Box-Behnken Design (BBD) was applied. GO;PANI adsorbent was characterized using thermogravimetric analysis (TGA), fourier transform-infrared spctroscopy (FT-IR), and field emission-scanning electron microscope (FE-SEM). As a result, it was confirmed that the GO;PANI adsorbent was successfully fabricated and that different distribution of VOCs were shown from plants we have investigated. The developed method was validated including limit of detection (LOD), limit of quantitation (LOQ), recovery and reproducibility.

BASIC VOLCANIC ROCK IN THE POHANG BASIN AND ITS STRATIGRAPHIC AND PETROGENETIC IMPLICATIONS

Hyesu YUN(윤혜수),Hyun-Koo Lee(이현구),Suckhwan SONG(송석환) 한국고생물학회 1995 고생물학회지 Vol.11 No.2