Korean pine nut oil replacement decreases intestinal lipid uptake while improves hepatic lipid metabolism in mice

한성림,Shuang Zhu,Soyoung Park,Yeseo Lim,Sunhye Shin 한국영양학회 2016 Nutrition Research and Practice Vol.10 No.5

BACKGROUND/OBJECTIVES: Consumption of pine nut oil (PNO) was shown to reduce weight gain and attenuate hepatic steatosis in mice fed a high-fat diet (HFD). The aim of this study was to examine the effects of PNO on both intestinal and hepatic lipid metabolism in mice fed control or HFD. MATERIALS/METHODS: Five-week-old C57BL/6 mice were fed control diets containing 10% energy fat from either Soybean Oil (SBO) or PNO, or HFD containing 15% energy fat from lard and 30% energy fat from SBO or PNO for 12 weeks. Expression of genes related to intestinal fatty acid (FA) uptake and channeling (Cd36, Fatp4, Acsl5, Acbp), intestinal chylomicron synthesis (Mtp, ApoB48, ApoA4), hepatic lipid uptake and channeling (Lrp1, Fatp5, Acsl1, Acbp), hepatic triacylglycerol (TAG) lipolysis and FA oxidation (Atgl, Cpt1a, Acadl, Ehhadh, Acaa1), as well as very low-density lipoprotein (VLDL) assembly (ApoB100) were determined by real-time PCR. RESULTS: In intestine, significantly lower Cd36 mRNA expression (P<0.05) and a tendency of lower ApoA4 mRNA levels (P = 0.07) was observed in PNO-fed mice, indicating that PNO consumption may decrease intestinal FA uptake and chylomicron assembly. PNO consumption tended to result in higher hepatic mRNA levels of Atgl (P = 0.08) and Cpt1a (P = 0.05). Significantly higher hepatic mRNA levels of Acadl and ApoB100 were detected in mice fed PNO diet (P<0.05). These results suggest that PNO could increase hepatic TAG metabolism; mitochondrial fatty acid oxidation and VLDL assembly. CONCLUSIONS: PNO replacement in the diet might function in prevention of excessive lipid uptake by intestine and improve hepatic lipid metabolism in both control diet and HFD fed mice.

학술대회 ( 포스터발표 ) : 2 - Acetylaminofluorene 과 butylated hydroxytoluene 의 성장기 쥐의 지질 과산화물 대사 효소에 미치는 영향

한성림,최혜미 한국영양학회 1989 총회 및 학술대회 Vol.- No.24

Nutritional Fat : Dietary Fat Modulation of Inflammation in Hypercholesterolemic Subjects

한성림 ( Sung Nim Han ) 한국지질동맥경화학회(구 한국지질학회) 2009 한국지질동맥경화학회 추계학술발표논문집 Vol.2009 No.-

체중 조절 프로그램에 참여한 비만 성인의 심리적 특성

김하나,한성림 대한비만학회 2014 The Korean journal of obesity Vol.23 No.4

Background: Psychological aspect is an important factor that needs to be taken into consideration in a weight management program for it to be successful and effective. This paper studies the psychological characteristics of the obese people. Methods: Forty two obese adults (body mass index 28.4 kg/m2, mean age of 29.4 years) and 23 normal weight adults (body mass index 21.5 kg/m2, mean age of 26.4 years) were studied. Beck Depression Inventory (BDI), Beck Anxiety Inventory (BAI), and Symptom Checklist (SCL)-90-R were used to measure depression and anxiety, and to evaluate psychological problems. Self-esteem, body shape satisfaction, and self-efficacy were assessed as variables for the psychological aspects of quality of life. Results: The obese group had higher scores for obsessive-compulsive, interpersonal sensitivity, paranoid ideation, and psychotic dimensions compared with the normal weight group. The obese group had lower self-esteem and lower body shape satisfaction than the normal weight group, however, obese group’s self efficacy was higher than the normal weight group. Conclusion: This study shows that the obese subjects have lower self-respect and lower body shape satisfaction. Therefore, efforts should be made to ease their anxiety regarding their body weight. Care and consideration for their feelings of inferiority should be exerted when weight management program is implemented. In addition, encouragement for self-control and self-determination is recommended. 배경: 본 연구에서는 체중 조절 프로그램의 효과를 높이기 위해 갖추어야 할 요인 중 하나로 심리적 정신적 특성이 반영되어야 한다는 필요성을 인식하고, 비만 성인의 심리 특성을 알아보고자 하였다. 방법: 비만인 성인(체질량지수 28.4 kg/m2, 평균 연령 29.4세) 42명과정상체중인 성인(체질량지수 21.5 kg/m2, 평균 연령 26.4세) 23명을대상으로 조사하였다. 우울과 불안, 정신적 증상들이 취약할 소지가있는지를 Beck의 우울척도 한국판, Beck의 불안척도 한국판, 간이정신진단검사를 사용하여 비교하였다. 또한 일상생활의 심리적 삶의 질과 관련이 있는 신체적 만족감과 자존감, 효능감 등을 측정하였다. 결과: 비만군과 정상체중군은 정신적 증상들이 모두 정상 범주에 해당하였다. 다만, 평균적으로 비만군이 정상체중군에 비하여 강박, 대인예민성, 편집성, 정신증이 높은 것으로 나타났다. 또한 비만군은 정상체중군에 비하여 신체적 만족도가 낮고 자존감이 낮으며 자기효능감은 높은 것으로 나타났다. 결론: 비만인은 자기를 존중하고 자신의 모습을 승인하는 것에 어려움을 겪을 가능성이 높으므로 체중과 관련된 근심과 걱정을 덜할 수있도록 도와주고, 자신의 어려움이나 열등감이 타인에게 비춰질까봐염려하는 것이 배려될 수 있도록 프로그램의 진행이 이뤄져야 할 것으로 보인다. 또한 자신의 통제 능력이나 자율성이 낮다고 지각할 수있으므로 이에 대한 격려가 이루어져야 할 것이다.

The Role of Vitamin D in Adipose Tissue Biology: Adipocyte Differentiation, Energy Metabolism, and Inflammation

박찬윤,한성림 한국지질동맥경화학회 2021 지질·동맥경화학회지 Vol.10 No.2

Adipose tissue is composed of diverse cell types and plays a major role in energy homeostasis and inflammation at the local and systemic levels. Adipose tissue serves as the main site for vitamin D storage and is among the most important extraskeletal targets of vitamin D which can modulate multiple aspects of adipose tissue biology. Vitamin D may exert inhibitory or stimulatory effects on adipocyte differentiation depending on cell type, stage of differentiation, and the treatment time point. Moreover, vitamin D controls energy metabolism in adipose tissue by affecting fatty acid oxidation, expression of uncoupling proteins, insulin resistance, and adipokine production. Adipose tissue inflammation can have a significant impact on the metabolic disorders often associated with obesity, and vitamin D can modulate the inflammatory response of immune cells and adipocytes within the adipose tissue. This review discusses the role of adipose tissue in vitamin D metabolism, as well as the regulatory role of vitamin D in adipocyte differentiation, adipose tissue energy metabolism, and inflammation, thereby providing insights into the importance of vitamin D in adipose tissue biology.

Isoegomaketone Upregulates Heme Oxygenase-1 in RAW264.7 Cells via ROS/p38 MAPK/Nrf2 Pathway

진창현,소양강,한성림,김진백 한국응용약물학회 2016 Biomolecules & Therapeutics(구 응용약물학회지) Vol.24 No.5

Isoegomaketone (IK) was isolated from Perilla frutescens, which has been widely used as a food in Asian cuisine, and evaluated for its biological activity. We have already confirmed that IK induced the HO-1 expression via Nrf2 activation in RAW264.7 cells. In this study, we investigated the effect of IK on the mechanism of HO-1 expression. IK upregulated HO-1 mRNA and protein expression in a dose dependent manner. The level of HO-1 mRNA peaked at 4 h after 15 μM IK treatment. To investigate the mechanisms of HO-1 expression modulation by IK, we used pharmacological inhibitors for the protein kinase C (PKC) family, PI3K, and p38 MAPK. IK-induced HO-1 mRNA expression was only suppressed by SB203580, a specific inhibitor of p38 MAPK. ROS scavengers (N-acetyl-L-cysteine, NAC, and glutathione, GSH) also blocked the IK-induced ROS production and HO-1 expression. Furthermore, both NAC and SB203580 suppressed the IK-induced Nrf2 activation. In addition, ROS scavengers suppressed other oxidative enzymes such as catalase (CAT), glutathione S-transferase (GST), and NADH quinone oxidoreductase (NQO-1) in IKtreated RAW264.7 cells. Taken together, it can be concluded that IK induced the HO-1 expression through the ROS/p38 MAPK/ Nrf2 pathway in RAW264.7 cells.

2-Acetylaminofluorene의 투여량에 따른 쥐간의 Monooxygenase에 관한 쇠기름 및 콩기름의 영향

김현덕,김현아,한성림,김경민,장경자,최혜미,Kim, Hyeon-Duck,Kim, Hyeon-A,Han, Sung-Nim,Kim, Kyung-Min,Jang, Kyung-Ja,Choi, Hay-Mie 생화학분자생물학회 1993 한국생화학회지 Vol.26 No.1

이유된 Sprague Dawley종 숫쥐를 식이 지방의 종류에 따라 쇠기름군과 콩기름군으로 나누고, 식이지방은 실험식이의 15% 수준으로 하여 8주 동안 사육하였으며 9주째 체중 kg당 25mg과 100mg의 2-AAF를 각각 3일간 연속 복강주사로 투여하고 다음날 희생시켰다. 간 micro­some으로부터 cytochrome P-450, cytochrome bs 함량, NADPH-cytochrome P-450 reductase 활성도, lipid peroxide 함량을 측정 비교하였다. 25mg AAF를 투여했을 때 cytochrome P-450 lipid peroxide 함량은 콩기름군이 쇠기름군보다 유의적 (p<0.05)으로 높았고, 100mg AAF를 투여 했을 때는 cytochrome P-450 함량은 감소했지만 cytochrome $b_5$ 함량은 증가하였다. NADPH-cytochrome P-450 reductase 활성도는 식이지방의 종류에 따라서는 차이가 없었으나 두 식이군 모두 2-AAF 투여량이 증가함에 따라 상승되었다. 이 결과로부터 식이지방의 불포화도가 높을수록 cytochrome P-450의 유도도 뿐아니라 100mg 2-AAF에 의한 손상도 촉진되는 것으로 보여지며, 지질과산화물과는 크게 연관성을 나타내지 않았다. Weanling Sprague Dawley male rats were fed the diet containing 15% beef tallow and soybean oil for 8 weeks. At 9th week, 2-AAF was injected everyday for 3 days by intraperitoneally at the levels of 25 mg and 100 mg/㎏ body weight and sacrificed next day. Cytochrome P-450 and cytochrome $b_5$ contents, NADPH-cytochrome P-450 reductase activity, lipid peroxide values were determined from isolated microsome. Cytochrome P-450 contents and lipid peroxide values were significantly higher in soybean oil group than beef tallow group by 25 mg 2-AAF injection, and cytochrome P-450 contents decreased but cytochrome $b_5$ contents increased in soybean oil group by 100 mg 2-AAF injection. NADPH-cytochrome P-450 reductase activities were not significantly different between dietary groups, but were elevated by 2-AAF injection and positively correlated to the levels of 2-AAF in each dietary group. These results suggest that soybean oil is more susceptible to the damage as well as induction of cytochrome P-450 by 2-AAF, and that lipid peroxide is not positively correlated to the cytochrome P-450 contents.

2 - Acetylaminofluorene 의 투여량에 따른 쥐간의 Monooxygenase 에 관한 쇠기름 및 콩기름의 영향

김현덕,김현아,한성림,김경민,장경자,최혜미 ( Hyeon Duck Kim,Hyeon A Kim,Sung Nim Han,Kyung Min Kim,Kyung Ja Jang,Hay Mie Choi ) 생화학분자생물학회 1993 BMB Reports Vol.26 No.1

Weanling Sprague Dawley male rats were fed the diet containing 15% beef tallow and soybean oil for 8 weeks. At 9th week, 2-AAF was injected everyday for 3 days by intraperitoneally at the levels of 25 ㎎ and 100 ㎎/㎏ body weight and sacrificed next day. Cytochrome P-450 and cytochrome b_5 contents, NADPH-cytochrome P-450 reductase activity, lipid peroxide values were determined from isolated microsome. Cytochrome P-450 contents and lipid peroxide values were significantly higher in soybean oil group than beef tallow group by 25 ㎎ 2-AAF injection, and cytochrome P-450 contents decreased but cytochrome b_5 contents increased in soybean oil group by 100 ㎎ 2-AAF injection. NADPH-cytochrome P-450 reductase activities were not significantly different between dietary groups, but were elevated by 2-AAF injection and positively correlated to the levels of 2-AAF in each dietary group. These results suggest that soybean oil is more susceptible to the damage as well as induction of cytochrome P-450 by 2-AAF, and that lipid peroxide is not positively correlated to the cytochrome P-450 contents.

거주지 주변의 식품환경과 비만의 관련성 연구

김윤정(Yoonjung Kim),한성림(Sung Nim Han) 대한지역사회영양학회 2016 대한지역사회영양학회지 Vol.21 No.5

Direct-to-Consumer Genetic Testing in Korea: Current Status and Significance in Clinical Nutrition

Lee Ga Young,한성림 한국임상영양학회 2021 Clinical Nutrition Research Vol.10 No.4

Direct-to-consumer genetic testing (DTC-GT) provides a means for consumers to gain insights into their genetic background and how it relates to their health without the involvement of medical institutions. In Korea, DTC-GT was introduced in 2016 in accordance with the legislation on Paragraph (3) 2 of Article 50 of the Bioethics and Safety Act. Only 12 genetic test items involving 46 genes were approved at first, but the approved items were expanded to 70 in November 2020. However, the genetic test items of DTC-GT services in Korea are still restricted to the wellness area, and access to disease risk related information is only permitted to medical institutions. Further, studies revealing the relationship between genotype differences and responses to nutrients, food components, or nutritional status are increasing, and this association appears to be robust for some genes. This strong association between genetic variations and nutrition suggests that DTC-GT can be used as an important tool by clinical nutritionists to gain insights into an individual's genetic susceptibilities and provide guidance on nutritional counseling and meal planning based on the patient's genetic information. This review summarized the history and current status of DTC-GT and investigated the relationship between genetic variations with associated phenotypic traits to clarify further the importance of DTC-GT in the field of clinical nutrition.