Combined Lowering Effects of Rosuvastatin and L. acidophilus on Cholesterol Levels in Rat

( Lijun Wang ),( Baihua Zhou ),( Xue Zhou ),( Yang Wang ),( Hongwei Wang ),( Shengying Jia ),( Zhipeng Zhang ),( Chao Chu ),( Jianjun Mu ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.3

Statins are a class of lipid-lowering drugs commonly used in the prevention of cardiovascular diseases. However, statin therapy presents many limitations, which have led to an increased interest in non-drug therapies, such as probiotics, to improve blood cholesterol levels. Indeed, probiotic strains such as Lactobacillus acidophilus have been found to improve blood lipid profiles, especially in reducing total cholesterol and LDL-C levels. In this study, we established a high-cholesterol rat model and studied the effect of Lactobacillus acidophilus administration alone or in combination with rosuvastatin. We were able to show that Lactobacillus exerts a cholesterol-lowering effect. Additionally, we observed that when administered together, rosuvastin and Lactobacillus exert a combined cholesterol-lowering effect. Altogether, our data advocate for the possibility of establishing probiotics as non-drug supplements for the treatment of hypercholesterolemia.

Color-Image Guided Depth Map Super-Resolution Based on Iterative Depth Feature Enhancement

( Lijun Zhao ),( Ke Wang ),( Jinjing Zhang ),( Jialong Zhang ),( Anhong Wang ) 한국인터넷정보학회 2023 KSII Transactions on Internet and Information Syst Vol.17 No.8

With the rapid development of deep learning, Depth Map Super-Resolution (DMSR) method has achieved more advanced performances. However, when the upsampling rate is very large, it is difficult to capture the structural consistency between color features and depth features by these DMSR methods. Therefore, we propose a color-image guided DMSR method based on iterative depth feature enhancement. Considering the feature difference between high-quality color features and low-quality depth features, we propose to decompose the depth features into High-Frequency (HF) and Low-Frequency (LF) components. Due to structural homogeneity of depth HF components and HF color features, only HF color features are used to enhance the depth HF features without using the LF color features. Before the HF and LF depth feature decomposition, the LF component of the previous depth decomposition and the updated HF component are combined together. After decomposing and reorganizing recursively-updated features, we combine all the depth LF features with the final updated depth HF features to obtain the enhanced-depth features. Next, the enhanced-depth features are input into the multi-stage depth map fusion reconstruction block, in which the cross enhancement module is introduced into the reconstruction block to fully mine the spatial correlation of depth map by interleaving various features between different convolution groups. Experimental results can show that the two objective assessments of root mean square error and mean absolute deviation of the proposed method are superior to those of many latest DMSR methods.

Experimental investigation of laser induced thermoforming of 5A06 aluminum alloy sheet

Lijun Yang,Yali Chen,Genwang Wang,Yujun Wei,Guanxin Chi,Yang Wang 한국생산제조학회 2016 한국생산제조시스템학회 학술발표대회 논문집 Vol.2016 No.10

Value Creation after Chinese Firms Acquire Financially Distressed Overseas Firms: Viva’s Acquisition of Clarks Group

Lijun Wang 아시아무역학회 2023 Journal of Asia Trade and Business Vol.10 No.2

Neuroprotection of Dexmedetomidine against Cerebral Ischemia-Reperfusion Injury in Rats: Involved in Inhibition of NF-κB and Inflammation Response

( Lijun Wang ),( Haiyan Liu ),( Ligong Zhang ),( Gongming Wang ),( Mengyuan Zhang ),( Yonghui Yu ) 한국응용약물학회 2017 Biomolecules & Therapeutics(구 응용약물학회지) Vol.25 No.4

Dexmedetomidine is an α2-adrenergic receptor agonist that exhibits a protective effect on ischemia-reperfusion injury of the heart, kidney, and other organs. In the present study, we examined the neuroprotective action and potential mechanisms of dexmedetomidine against ischemia-reperfusion induced cerebral injury. Transient focal cerebral ischemia-reperfusion injury was induced in Sprague-Dawley rats by middle cerebral artery occlusion. After the ischemic insult, animals then received intravenous dexmedetomidine of 1 mg/kg load dose, followed by 0.05 mg/kg/min infusion for 2 h. After 24 h of reperfusion, neurological function, brain edema, and the morphology of the hippocampal CA1 region were evaluated. The levels and mRNA expressions of interleukin-1b, interleukin-6 and tumor nevrosis factor-α as well as the protein expression of inducible nitric oxide synthase, cyclooxygenase-2, nuclear factor-κBp65, inhibitor of κBα and phosphorylated of κBα in hippocampus were assessed. We found that dexmedetomidine reduced focal cerebral ischemia-reperfusion injury in rats by inhibiting the expression and release of inflammatory cytokines and mediators. Inhibition of the nuclear factor-κB pathway may be a mechanism underlying the neuroprotective action of dexmedetomidine against focal cerebral I/R injury.

Neuroprotection of Dexmedetomidine against Cerebral Ischemia-Reperfusion Injury in Rats: Involved in Inhibition of NF-κB and Inflammation Response

Wang, Lijun,Liu, Haiyan,Zhang, Ligong,Wang, Gongming,Zhang, Mengyuan,Yu, Yonghui The Korean Society of Applied Pharmacology 2017 Biomolecules & Therapeutics(구 응용약물학회지) Vol.25 No.4

Dexmedetomidine is an ${\alpha}2$-adrenergic receptor agonist that exhibits a protective effect on ischemia-reperfusion injury of the heart, kidney, and other organs. In the present study, we examined the neuroprotective action and potential mechanisms of dexmedetomidine against ischemia-reperfusion induced cerebral injury. Transient focal cerebral ischemia-reperfusion injury was induced in Sprague-Dawley rats by middle cerebral artery occlusion. After the ischemic insult, animals then received intravenous dexmedetomidine of $1{\mu}g/kg$ load dose, followed by $0.05{\mu}g/kg/min$ infusion for 2 h. After 24 h of reperfusion, neurological function, brain edema, and the morphology of the hippocampal CA1 region were evaluated. The levels and mRNA expressions of interleukin-$1{\beta}$, interleukin-6 and tumor nevrosis factor-${\alpha}$ as well as the protein expression of inducible nitric oxide synthase, cyclooxygenase-2, nuclear factor-${\kappa}Bp65$, inhibitor of ${\kappa}B{\alpha}$ and phosphorylated of ${\kappa}B{\alpha}$ in hippocampus were assessed. We found that dexmedetomidine reduced focal cerebral ischemia-reperfusion injury in rats by inhibiting the expression and release of inflammatory cytokines and mediators. Inhibition of the nuclear factor-${\kappa}B$ pathway may be a mechanism underlying the neuroprotective action of dexmedetomidine against focal cerebral I/R injury.

Polyelectrolyte Complex Formation between Cationic Fixing Agent and Dissolved Substances in Papermaking

Lijun Wang,Yiqian Zhang,Pedram Fatehi 한국펄프·종이공학회 2016 한국펄프·종이공학회 학술발표논문집 Vol.2016 No.10

1. Formation of DS-fixing agent PEC highly depends on the DS components, fixing agent molecular weight and its dosage, as very different phenomena can be seen in this study for different DS components, for polyamines with different molecular weight, and for the same polyamine used at a different dosage. 2. For fixing agent with enough low molecular weight and at enough low dosage, it can form loose and dispersible DS-fixing agent PECs, and increasing its dosage will produce denser and smaller PECs; for fixing agent with enough high molecular weight, it can directly form dense DS-fixing agent PECs, and increasing its dosage mainly increases the size of PECs; for fixing agent with medium molecular weight, its behaves between the two cases described above. 3. Although hemicelluloses modeled DS water showed that a small part of PECs formed are detectable by FBRM, the performance of DCS water 2 used in this study showed that DS-fixing agent PECs alone are generally too small to be detected by FBRM. 4. Based on the performance of DCS water 1 and DCS used in this study, it can be concluded that small CS particles undetectable by FBRM can be the “seeds” for producing larger particles detectable by FBRM, either by agglomerating the small CS particles or by depositing DS-fixing agent PECs onto the small CS particles. For the formation of FBRM-detectable particles, the agglomeration of medium sized CS particles (between 125nm-470nm in this study), or deposition of DS-fixing agent PECs onto such particles, seems critical.

Genome-wide DNA methylation pattern in a mouse model reveals two novel genes associated with Staphylococcus aureus mastitis

Wang, Di,Wei, Yiyuan,Shi, Liangyu,Khan, Muhammad Zahoor,Fan, Lijun,Wang, Yachun,Yu, Ying Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.2

Objective: Staphylococcus aureus (S. aureus) is one of the major microorganisms responsible for subclinical mastitis in dairy cattle. The present study was designed with the aim to explore the DNA methylation patterns using the Fluorescence-labeled methylation-sensitive amplified polymorphism (F-MSAP) techniques in a S. aureus-infected mouse model. Methods: A total of 12 out-bred Institute of Cancer Research female mice ranging from 12 to 13 weeks-old were selected to construct a mastitis model. F-MSAP analysis was carried out to detect fluctuations of DNA methylation between control group and S. aureus mastitis group. Results: Visible changes were observed in white cell counts in milk, percentage of granulocytes, percentage of lymphocytes, CD⁴⁺/CD⁸⁺ ratio (CD⁴⁺/CD⁸⁺), and histopathology of mice pre- and post-challenge with S. aureus. These findings showed the suitability of the S. aureus-infected mouse model. A total of 369 fragments was amplified from udder tissue samples from the two groups (S. aureus-infected mastitis group and control group) using eight pairs of selective primers. Results indicated that the methylation level of mastitis mouse group was higher than that in the control group. In addition, NCK-associated protein 5 (Nckap5) and transposon MTD were identified to be differentially methylated through secondary polymerase chain reaction and sequencing in the mastitis group. These observations might play an important role in the development of S. aureus mastitis. Conclusion: Collectively, our study suggests that the methylation modification in Nckap5 and transposon MTD might be considered as epigenetic markers in resistance to S. aureus-infected mastitis and provided a new insight into S. aureus mastitis research in dairy industry and public health.

Polyelectrolyte Complex Formation between Cationic Fixing Agent and Dissolved Substances in Papermaking

Lijun Wang,Yiqian Zhang,Pedram Fatehi 한국펄프·종이공학회 2016 펄프.종이기술 Vol.48 No.6

Dissolved substances (DS) in papermaking suspension contribute a large portion of negativity to the total dissolved and colloidal substances (DCS). Compared to colloidal substances (CS), dissolved substances are clearly more difficult to be removed by fixing agents (FA). Formation of polyelectrolyte complexes (PECs) between fixing agents and dissolved substances plays a very important role in clarifying the mechanism of a fixing agent acting on DCS. This study, therefore, focused on the characteristics of PECs formed between fixing agents and dissolved substances. Three polyamines (PA) with different molecular weight and slight different charge density were synthesized and used as fixing agents, in four different kinds of water including, two kinds of DCS water separated from a same deinked pulp (DIP) but with different size and distribution of CS particles, and two kinds of DS water modeled by oxidized starch (OS) and hemicelluloses (HC). The formation of PEC in these systems and their corresponding characteristics were characterized using focused beam reflectance meter (FBRM), laser particle size analyzer (LPSA), turbidity meter, and charge detection. From the results, it is concluded that for a given DS component and a certain kind of fixing agent, the formation of DSfixing agent PECs highly depended on the molecular weight of the fixing agent and its dosage. A fixing agent with enough low molecular weight could form big but loose and dispersible DS-FA PECs, increasing its dosage would produce denser and smaller PECs; and a fixing agent with enough high molecular weight could directly form dense PECs, increasing its dosage increased the size of PECs. Depositing DS-FA PECs onto small CS particles also played an important role in forming new CS particles with larger sizes, and in this case, a fixing agent with higher molecular weight tended to produce more large particles.

Temperature dependent development of black soldier fly, Hermetia illucens (L.) (Diptera: Stratiomyidae) from Yangtze River Delta region of China

Wang Yinghui,Zhang Yanan,Wang Jiangfeng,Kang Chengtao,Hu Gengwang,Guo Yi,Chen Jie,Yang Lijun,Wang Yu 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.4

The black soldier fly, Hermetia illucens (L.) (Diptera: Stratiomyidae), isomnivorous andwidely distributed in the world. Black soldier flies usually colonize corpse at a relatively late stage and have a longer developmental time compared to other flies. It can serve as a good supplementary indicator for estimating a longer minimum postmortem interval (PMI min ) in forensic investigations. In this paper, the development of H. illucens was investigated at six constant temperatures between 19 and 34 ◦ C. Under temperatures of 19, 22, 25, 28, 31, and 34 ◦ C, the mean developmental time from eggs to adults was 90.96, 58.29, 54.61, 42.33, 32.33 and 34.52 days, respectively. Developmental time and accumulated degree days results were used to construct thermal sum mation model and isomorphen diagram. The calculated values of lower developmental thresholds and thermal summation constant estimated by a linear model were 11.88 ◦ C and 650.89 degree days, respectively. Through a nonlinear model, the upper lethal developmental threshold temperature, intrinsic optimum temperature and lower developmental thresholds were estimated to be 35.18, 20.50 and 13.17 ◦ C, respectively. A growth curve, an equation and isomegalen diagram based on them show the relationship between developmental time and larval body length. The larval body length increased with increased developmental time and eventually pla teaued, revealing an S-shape growth curve. These results provide important basic developmental data for H. illucens, which can be used to estimate the PMI min .