Estrogen administration during superovulation increases oocyte quality and expressions of vascular endothelial growth factor and nitric oxide synthase in the ovary

Ha, Choong-Sik,Joo, Bo-Sun,Kim, Seung-Chul,Joo, Jong-Kil,Kim, Hwi-Gon,Lee, Kyu-Sup Blackwell Publishing Asia 2010 JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH -TO Vol.36 No.4

<P>Abstract</P><P>Aims: </P><P>This study investigated whether estrogen administration during superovulation enhances oocyte quality using a mice model. We also investigated whether this estrogen treatment regulates the expressions of angiogenic factors, such as vascular endothelial growth factor (VEGF) and endothelial nitric oxide synthase (eNOS), in the ovary.</P><P>Method: </P><P>Female mice were co-injected with various doses of estrogen (1 µM, 10 µM and 100 µM) and pregnant mare serum gonadotrophin during superovulation, followed by human chorionic gonadotrophin injection 48 hours later. Then they were mated with individual males. After 18 hours, zygotes were flushed and cultured to blastocyst. The expression of VEGF and eNOS in the ovary was examined using Western blot and immunohistochemistry. The control group was superovulated without estrogen.</P><P>Results: </P><P>Both numbers of ovulated zygotes and the rate of embryo development to blastocyst were significantly increased in the 1-µM estrogen dose compared to the control group. VEGF and eNOS expressions were stimulated by estrogen treatment. In particular, VEGF expression was significantly increased at 1-µM estrogen concentration, whereas, eNOS expression was significantly increased in all estrogen concentrations compared to controls.</P><P>Conclusions: </P><P>The study showed that estrogen co-injection during superovulation increased the ovarian response, embryo developmental competence and expressions of VEGF and eNOS in the ovary.</P>

짧은 추적기간 중 형태학적 변화를 보인 과형성 위용종증 1 예

하창원,신경한,주광로,김성수,박상선,강승호,김원천,김충현,엄기성 대한소화기내시경학회 2001 Clinical Endoscopy Vol.22 No.6

Hyperplastic polyps are often multiple and by far the most frequent lesions among gastric polyps, contributing for about 90% of the polypoid lesions observed in the stomach. However, hyperplastic gastric polyposis is rare and was not exactly evaluated for natural course. These lesions are considered reactive and not genetically determined conditions. So it has been claimed that the hyperplastic polyps and hyperplastic polyposis are non-neoplastic lesions that result from regenerative hyperplastic reaction to inflammation and erosion. Recently we experienced a hyperplastic gastric polyposis with morphological changes during short follow-up period and reported herein.

Differences in the protein expression levels of Trx2 and Prx3 in the hippocampal CA1 region between adult and aged gerbils following transient global cerebral ischemia

LEE, CHOONG HYUN,PARK, JOON HA,CHO, JEONG-HWI,AHN, JI HYEON,BAE, EUN JOO,WON, MOO-HO D.A. Spandidos 2015 MOLECULAR MEDICINE REPORTS Vol.12 No.2

<P>The thioredoxin (Trx) and peroxiredoxin (Prx) redox system is associated with neuronal damage and neuroprotective effects via the regulation of oxidative stress in brain ischemia. In the present study, ischemia-induced changes in the protein expression levels of Trx2 and Prx3 in the stratum pyramidale (SP) of the hippocampal CA1 region were investigated in adult and aged gerbils, subjected to 5 min of transient global cerebral ischemia, using immunohistochemistry and western blot analysis. In the adult ischemia-group, minimal Trx2 immunoreactivity was detected in the SP 2 days after ischemia-reperfusion. In the aged animals, the Trx2 immunoreactivity in the sham-group was marginally lower compared with that in the adult sham-group. In the aged ischemia-group, Trx2 immunoreactivity in the SP was significantly higher 1, 2 and 4 days post-ischemia, compared with that in the adult ischemia-group and, in the 5 days post-ischemia group, Trx2 immunoreactivity was significantly decreased in the SP. Prx3 immunoreactivity in the SP of the adult ischemia-group was significantly decreased from 4 days after ischemia-reperfusion. In the aged animals, Prx3 immunoreactivity in the sham-group was also marginally lower compared with that in the adult sham-group. Prx3 immunoreactivity in the aged ischemia-group was also significantly higher 1, 2 and 4 days post-ischemia, compared with the adult ischemia-group; however, the Prx3 immunoreactivity was significantly decreased 5 days post-ischemia. The western blot analyses revealed that the pattern of changes in the protein levels of Trx2 and Prx3 in the adult and aged hippocampal CA1 region following ischemic damage were similar to the results obtained in the immunohistochemical data. These findings indicated that cerebral ischemia lead to different protein expression levels of Trx2 and Prx3 in the hippocampal CA1 region between adult and aged gerbils, and these differences may be associated with more delayed neuronal death in the aged gerbil hippocampus following transient global cerebral ischemia.</P>

A Concentrator Based Optical Packet Switch Using a Distributed Optical Contention Resolution

Hyun Ha Hong(홍현하),Jee Yon Choi,Choong Reul Yang,Phil Joo Moon,Chang-Hee Lee 한국통신학회 1999 한국통신학회 학술대회 및 강연회 Vol.1999 No.4

저등급 자궁내막간질육종

박충학(Choong Hak Park),주선하(Seon Ha Joo),박호범(Ho Beom Park),서영훈(Young Hoon Suh),김동희(Dong Hee Kim) 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.1

Uterine sarcomas are relatively rare tumors of mesodermal origin that constitute 2-6% of uterine malignancies. Uterine sarcomas include endometrial stromal sarcoma (ESS), leiomyosarcoma (LMS), malignant mixed m llerian tumor (MMMT) of both homologous and heterologous type, pure heterologous sarcomas, blood vessel sarcomas, and lymphoma. Endometrial stromal tumors are divided into three types: 1) endometrial stromal nodule, 2) low-grade stromal sarcoma or endolymphatic stromal myosis, and 3) endometrial stromal sarcoma. The low grade endometrial stromal sarcoma is very rare. It shows occasional local recurrence, which might arise from endometrial stroma, from adenomyosis, and rarely from endometriosis. We have experienced a case of low grade endometrial stromal sarcoma of the uterus in a 54-year-old woman, which is presented with a review of literatures.

Abscess followed by spontaneous bacterial peritonitis

( Jeongha Ha ),( Eileen L. Yoon ),( Ji Young Park ),( Jeongmin Choi ),( Tae Joo Jeon ),( Won Chang Shin ),( Won-choong Choi ) 대한내과학회 2015 대한내과학회 추계학술대회 Vol.2015 No.1

In most cases, spontaneous bacterial peritonitis (SBP) shows good response to empirical antibiotics. Therefore SBP complicated by intra-abdominal abscess is not frequent. We report the first case of intra-abdominal abscess followed by spontaneous bacterial peritonitis. A 57-year-old female patient with alcoholic liver cirrhosis was transferred from the other secondary hospital for upper gastrointestinal bleeding while she had been treated with 3rd cephalosporin for 5 days due to spontaneous bacterial peritonitis. The initial ascitic fluid analysis showed pH 7.0, RBC ≥ 20,000/mm3, WBC 9,280/mm3, neutrophil 78% which implied uncontrolled SBP by 3rd cephalosporin. After 2 days of carbapenem use, the corrected neutrophil count in ascites was 1133/mm3, decreased by 83% of the initial neutrophil count. The causative micro-organism was not confirmed even with repetitive culture of blood and ascites specimens. Even with 3 weeks’ of continuous carbapenem and additive vancomycin administration, intermittent fever upto 38.6℃ persisted. Neutrophil count in ascites fluctuated between 0 and 2,969 and more than two of the Runyon’s criteria were present. CT scan showed no evidence of bowel perforation or organizing abscess. Poor liver function of Child Pugh class C, non-toxic condition of the patient, and no evidence of secondary bacterial peritonitis refrained us from performing explorative laparoscopy. She was discharged with prescription of oral antibiotics. After a month, she complained of heat sense in the epigastrium with tenderness in the area. CT scan was performed and there was a large intra-abdominal abscess without any focus of bowel perforation. The ascitic fluid analysis was compatible with abscess. She was treated with percutaneous catheter drainage and empirical antibiotics. After 5 months, intra-abdominal abscess was completely resolved. Difficult-to-treat SBP can be transformed to abscess under the influence of long-term antibiotic treatment. Therefore we suggest careful monitoring of ascitic fluid analysis and low threshold for performing percutaneous drainage is crucial in the care of SBP which does not respond to empirical antibiotic treatment.

게잡이 원숭이에 있어 rHuEPO의 4주간 투여 후 혈액학 및 조직병리학적 변화

김충용(Choong-Yong Kim),윤석주(Seok-Joo Yoon),김용범(Yong-Bum Kim),하창수(Chang-Su Ha),김달현(Dal-Hyun Kim),권명상(Myung-Sang Kwon),한상섭(Sang-Seop Han) 한국독성학회 2005 Toxicological Research Vol.21 No.3

We investigated effects of recombinant human erythropoietin (rHuEPO) on body weight, organ weight, hematology, serum biochemistry, and histopathology in 6 male cynomolgus monkeys (Macaca fascicularis). Six monkeys, composed of treatment and control groups, were intravenously administered 3 times per week at doses of 0 and 2730 IU/ 0.1 ㎖/㎏ with rHuEPO for 4 weeks. rHuEPO treatment did not show any change in body weights for 4 weeks and caused an increase of organ weight in spleen, salivary gland, kidney, and liver. rHuEPO treatment increased significantly mean corpuscular hemoglobin concentration, reticulocyte, and red cell distribution width from day 3 of the study and red blood cells, hemoglobin, and hematocrit levels were increased significantly from day 7 or 10 up to day 28. For an increase in red cell distribution width, it was found to a large extent on day 7, day 10, and day 14, while was found to a small extent on day 21 and day 28. It increased platelet distribution width on day 10, day 14, day 21, and day 28. Histopathological examination showed that rHuEPO treatment caused an extramedullary hemopoiesis in spleen and bone marrow hyperplasia in sternum and femur. The results indicated that rHuEPO treatment caused an increase in platelet-and RBC-related parameters, extramedullary hemopoiesis of spleen, and bone marrow hyperplasia of sternum and femur. The present study will be valuable in the proper interpretation and validation of general toxicology studies for biogeneric drugs including rHuEPO in cynomolgus monkeys.

잠복성 간질에 대한 항간제 투여의 뇌파상 효과

박충서,변영주,하정상 영남대학교 의과대학 1985 Yeungnam University Journal of Medicine Vol.2 No.1

1985년 1월부터 10월까지 본 영남대학교 의과대학 부속병원 신경과 외래를 찾은 두통환자 중에서 뇌파상 중등도 이상의 이상이 있는 환자가 580명이었다. 이들 중에서 간질의 간헐기 뇌파와 유사한 소견을 보이는 162명 환자들을 대상으로 투여한 항간제 및 뇌영양제 등을 단독 혹은 병용 투여한 후 치료효과를 분석한 결과 항간제투여군 29예에서 호전이 16예, 무변화가 13예이고, 항간제를 쓰지 않고 뇌 영양제를 사용했던 18예에서는 전예에서 호전을 보았다. 한편 항간제를 사용한 33예와 사용하지 않은 35예에서는 뇌파추적을 하지 못하였다. 수가 적어서 통계학적인 고찰은 하지 않았으며 앞으로 계획적인 계속 연구가 요망된다. The clinical state with EEG pattern similar to interval discharge of epileptics is named as latent epilepsy, which does not necessarily mean that the patient will develop epilepsy later. However, since there is possibility of developing epilepsy on later date, antiepileptic mainly dilantin was tried to control the abnormal EEG. Since january to October 1985, total 580 headache sases with more than moderately abnpormal EEG visited the Neurology clinic. Among them 162 cases with interval seizure pattern(ISP) of epilepsy were selected for the study. The main ISP was 1. diffuse theta and/or delta bursts and 2. spikes. Since the study is only analysis of clinical treatment of 162 cases without previous planning based on financial aid, about 30% of the patients did not return after the 1st EEG examination, in 42% failed to follow the EEG after the treatment and only remaining 28% of the cases were studied. Among 29 patients who were treated with Dilantin 100㎎ tid po, 16 improved and 13 not. Of the 13, 4 showed partial improvement and partial progression. Case 1. In 4 weeks of antiepileptic theray(AR), spikes disappeared but in 2 months developed bursts. Case 2. In 17days of AR,spikes and bursts disappeared but in 3 months bursts reccured. Case 3. In 1 week of AR, bursts disappeared but spikes developed. Case.4. In 3 months of AR, no change of spikes and bursts and she discontinued the AR. In 6 months she developed grandmal seizure. Eighteen cases, treated with other drugs except antiepileptics, all showed improvement. The other drugs were vincaprol, polygammalon, aronamin, ATP and hydergine. The improved cases had spikes more often than theta bursts. In view of the small number of the cases due to dropping most patients out of present study, it is considered meaningless to perform statistical analysis. Further well planned study with more patients is to be expected.

유도전동기의 디지탈 속도 제어

이충환(Choong-Hwan Lee),김상봉(Sang-Bong Kim),하주식(Joo-Sik Ha) 제어로봇시스템학회 1992 제어로봇시스템학회 국내학술대회 논문집 Vol.1 No.1

유도전동기의 디지탈 속도 제어

이충환(Choong-Hwan Lee),김상봉(Sang-Bong Kim),하주식(Joo-Sik Ha) 대한전자공학회 1992 대한전자공학회 학술대회 Vol.1992 No.10

In recent years, induction motor is applied for several industrial actuating parts instead of direct current motor because of the robust construction. nonexpensive and maintenance-free actuator etc. and having capability of speed control according to development of power electronics and microprocessor techniques. In the paper, a microprocessor-based digital control approach for speed control of induction motor system is presented by considering a simple modeling equation as the system expression equation of induction motor and using the self tuning control and torque feedforward control method. As the model equation of the induction motor system, we use a second order differential equation which is well known in the modeling equation of the mechanical systems. The mechanical modeling equation is induced from the control theory stand point such that we can describe usually the motor system connected by inverter, generator and load etc. The effectiveness of the control system composed by the above mentioned design concept is illustrated by the experimental result in the presence of step reference change and generator load variation.