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박경식,김동한,이규식 한양대학교 의과대학 1984 한양의대 학술지 Vol.4 No.1
Development and differentiation of mammalian kideney has been studied by many investigators. Hitreto, it has been known that a high vertebrate's kidney was differentiated from as a metanephros. As a result of the study of most investigators on developmental processes of metanephros in man rodents, they insisted that morphological development and differentiation of nephrons and collecting ducts should occur in the latter one quarter period of the fetal ages. But the initiation and completion time of renal function following its morphological development and differentiation have not been investigated perfectly. The author has determined the relationships of morphological and functional development of kidney were able to be revealed the studies of hydrolyzing phosphatase activities on the developing renal tissues. Therefore, in order to investigate the phosphatase activities of renal tissue following the development and differentiation of rate kidney, after interbreeding of healthy rats, fetuses of the 15th, 17th, 19th and newborns of the 1st, 4th, 7th and 10th day were sacrificed and were resected renal tissues. Histological preparations were prepared by way of hematoxylin and eosin stain for morphological changes of rat renal tissues, and Gomori's method(1941) for the detection of the activities of alkaline phosphatase, Gomori's method(1939) for the detection of the activities of acid phosphatase and Wachstein and Meisel's method(1957) for the detection of activities of ATPase were used, respectively. All of the preparations were observed with light microscope. The results were as follows; 1. The early differentiation of rat kidney began with glomerulus formation at the 17th day of fetal age, and morphological development was completed at the 21th day of fetal age, and lobulation of kidney was observed at the 7th day of neonatal age. 2. In the fetal kidney of the 17th gay, alkaline phosphatase activities were observed initially in the proximal tubules surrouding glomeruli in the cortico-medullary junction, and the activities were increased at the 17th day of fetus. At the 21th day of fetus, the strength of the enzyme activity was increased at the same time as renal tubule which showed alkaline phosphatase activity was increased in the cortex. In the each newborn kidney, the enzyme activities were continuously increased in the proximal tubules at the 1st, 4th, 7th and 10th day of newborns. The renal corpuscle and ascending straight tubule of the Henle's loop showed trace activities. 3. Acid phosphatase activity was shown at renal tubles and glomeruli during the fetal age after the 17th day of fetus, and moderate reaction of the enzyme was shown in the renal tubules, glomeruli, medullary rays and Henle's loops after birth. 4. Adenosine triphosphatase showed moderate reaction in the glomerulus at the 17th day and 19th day of fetus, and strong reaction was shown at the 21th day of fetus, and the ATPase activities of medullary rays showed trace reaction after birth. According to the beginning and change of the enzyme activity as mentioned above, it was supposed that the development of rat kidney should begin at begin at the 17th day of fetus. In the 21th day of fetus, diuresis and resorption were accoomplished mostly in the glomerulus and proximal tubule, and resorption of the Henle's loop began just before and after the birth.
마이크로파 여기 플라즈마광 생성을 위한 도파관 시스템 설계에 관한 연구
전상재,전후동,송창현,하석영,이승혁,이태호,박의준 금오공과대학교 2005 論文集 Vol.26 No.-
In this paper, the plasma lighting system(PLS) excited by the commercial high power magnetron is developed. The design concepts are based on maximizing the huninous efficacy in conjunction with the miniaturization of waveguide system Furthermore the fine tuning is simplified by using only one stub, and the impedance matching is maximized by introducing the tapering technique. The experimental results show that the luminous efficacy can be dramatically improved by the proposed design method.
김동석 ( Dong Seog Kim ),박혜진 ( Hye Jin Park ),윤종문 ( Jong Mun Yoon ),박용석 ( Yong Seok Park ),박영식 ( Young Seek Park ) 한국환경과학회 2014 한국환경과학회지 Vol.23 No.6
In this study, we examined the effect of cathode from electrolysis reactor for treating ballast water. We are going to select a suitable cathode for seawater electrolysis after considering the effect on the generation of the oxidant of cathode and the electrode deposition materials adhering to the surface of cathode. Anode is Ru-Ti-Pd electrode and cathode are Ti, Pt, JP520 (Ni-Pt-Ce) electrodes. Using the cathode of the three types, experiments were conducted to examine the effects of TRO (total residual oxidants) generation concentration and RNO (N, N-Dimethyl-4-nitrosoaniline, indicator of the generation of OH radical) degradation concentration (in 1, 35 psu), ohmic drop, FESEM(field emission scanning electron microscope) observation of cathode surface and EDX (energy dispersive X-ray spectroscopy) measurements of attached fouling material. The results showed that TRO generation concentration and RNO degradation concentration in according to each type of cathode are not different. The attached fouling materials were observed on the surface of Ti and the JP520 electrode by the observation of SEM after electrolysis for two hours, but it was not observed on the surface of Pt electrode. When considering the surface ohmic drop of cathode and the attached fouling materials, Pt electrode was judged as the excellent cathode.
플라즈마 공정을 이용한 Fusarium oxysporum과 Phytophthora capsici의 불활성화에 관한 연구
박영식 ( Young Seek Park ),이종휘 ( Jong Hwi Lee ),김호진 ( Ho Jin Kim ),백상은 ( Sang Eun Back ),박지민 ( Ji Min Park ),문휘 ( Whee Moon ),김동석 ( Dong Seog Kim ) 대구가톨릭대학교 자연과학연구소 2011 자연과학연구논문집 Vol.9 No.1
The purpose of this study is to figure out the inactivation of Fusarium oxysporum and phyophthora capsici using plasma process in conditions(primary voltage, secondary voltage, amount of air, concentration of NaCl, and pH). When both primary voltage and secondary voltage was increased, the inactivation of F. oxysporum was increased. Also, When both amount of air supply and the concentration of NaCl was increased, the inactivation of F. oxysporum was increased. However, when pH was decreased, the inactivation of F. oxysporum was increased. And, by adding P. capsici to this study, We can compare P. capsici with F. oxysporum.
경남 북부지역 오리 분변에서 분리된 Campylobacter spp.의 항생제 내성
김형수 ( Hyeong-su Kim ),서덕진 ( Deok-jin Seo ),성민호 ( Min-ho Seong ),한권식 ( Kwon-seek Han ),박정용 ( Jung-yong Park ),정명호 ( Myeong-ho Jeong ),박동엽 ( Dong-yeop Park ),박동주 ( Dong-ju Park ),고필옥 ( Phil-ok Koh ) 한국가축위생학회 2017 韓國家畜衛生學會誌 Vol.40 No.2
The purpose of this study was to investigate prevalence and antimicrobial resistance patterns of Campylobacter spp. from duck feces in northern area of the Gyeongnam province, Korea. Samples of 121 duck feces were taken from April to December 2014 for this survey. Samples were examined by bacteria isolation and reverse transcriptase-polymerase chain reaction assay for detection of Campylobacter spp. Campylobacter were isolated in 37 samples (30.6%). Among these samples, C. jejuni and C. coli were isolated in 35 samples and 2 samples, respectively. Minimum inhibitory concentration (MIC) test is performed to investigate antimicrobial resistance patterns of Campylobacter spp. C. jejuni were resistant to ciprofloxacin (85.7%), nalidixic acid(82.9%), tetracycline (77.1%), gentamicin (57.1%), azithromycin (40.0%), clindamycin (34.3%), erythromycin (22.9%), and florfenicol (8.6%). These data support a database of pollution and antimicrobial resistance of Campylobacter spp. from duck feces and provide a basic information of reducing the secondary damage of antibiotic misuse.
Microarray analysis of gene expression in 3-methylcholanthrene-treated human endothelial cells
Park, Hye Rim,Yang, Hana,Kim, Gun-Dong,Son, Gun Woo,Park, Yong Seek 대한독성유전단백체학회 2014 Molecular & cellular toxicology Vol.10 No.1
Cigarette smoke has many toxic components that can lead to the disruption of various cellular processes. Increase in oxidative stress due to cigarette smoke causes production of reactive oxygen species, which induce vascular disease. 3-methylcholanthrene (3MC) is an environmental contaminant derived from the incomplete combustion of cigarette smoke and is one of the major polycyclic aromatic hydrocarbons. 3MC activates the aryl hydrocarbon receptor and contributes to carcinogenesis and vascular disease. The present study investigated gene expression in 3MC-treated human umbilical vein endothelial cells by microarray gene expression profiling. We found 1,279 and 717 genes with altered gene expression within 24 h of 100 nM and $1{\mu}m$ 3MC treatment, respectively; these genes were distributed into distinct functional groups. Some of the genes including ICAM1, PTGS2, APOL3, and GPX-1, were related to cardiovascular disease. Our results support the hypothesis that 3MC-stimulated gene expression in endothelial cells may play a role in the development of cardiovascular disease.
연구논문 : COD Removal of Rhodamine B from Aqueous Solution by Electrochemical Treatment
( Dong Seog Kim ),( Young Seek Park ) 한국환경과학회 2012 한국환경과학회지 Vol.21 No.6
This study elucidates the COD removal of dye (Rhodamine B) through electrochemical reaction. Effects of current density (7.2 to 43.3 mA/cm2), electrolyte type (NaCl, KCl, Na2SO4, HCl), electrolyte concentration (0.5 to 2.0 g/L), air flow rate (0 to 4 L/min) and pH (3 to 11) on the COD removal of Rhodamine B were investigated. The observed results showed that the increase of pH decrease the COD removal efficiency. Whereas, the increase of current density, NaCl concentration and air flow rate caused the increase of the COD removal of Rhodamine B.
박경식,이춘용,이남규,우영남,김동한 최신의학사 1978 最新醫學 Vol.21 No.11
One patient with accessary urethra is reported. The anatomic feature was incomplete and its opening was dorsum: The proximal part was blind. We diagnosed by method of retrograde urethrogram and the biopsy report was normal urethral muosa. We had done extirpation and the result was very good, follow up study. Our report of incomplete duplicated urethra is 1st cas in Korea and it having been reported in approximately 138 patients (include our case) in the world since Aristotle.