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인간 재조합 인터루긴-32 면역조절작용에 대한 유세포 분석
이광수,김영관,채정일,심정현,김은미,강형식,김수현,윤도영,명평근 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-
Xenotransplantation of porcine organs has the potential to overcome the severe shortage of human tissues and organ available for human transplantation. however, it remains various hurdles for clinical xenotransplantation. In pig and mouse xenotransplantation, porcine xenograft evoke a strong cellular rejection response in immunocompetent host and grafts are destroyed within a week. This cellular immune response could involved both T cells and NK cells. A number of groups have shown that human NK cells can recognize and damage porcine endothelial cells. In addition, human T cells can respond to porcine endothelial cells through both direct and indirect mechanisms. Cellular rejection of porcine tissues requires T cells, particularly CD4^(+) cells. A new cytokine recombinant human interleukin-32α,β(IL-32α,β) has a role innate and acquired immune system. In order to investigate the role of recombinant mouse IL-18 and recombinant human IL-32α,β in xenograft rejection, we transplanted the PK(15) cells to C57BL/6 mice with or without intraperitoneal injection of recombinant mouse IL-18 or recombinant human IL-32 α,β. It was analyzed the population of NK cell, T cell and B cell in the C57BL/6 mice transplanted with PK(15) cells and recombinant mouse IL-18 or recombinant human IL-32α,β by flow cytometry analysis. As a result, lymph node and thymus of PK15/IL18, PK15/IL32α and PK15/IL32β injected group were increased to T cell activation population than normal injected groups. CD8^(+) T cells were decreased in lymph node of PK15/IL18, PK15/IL32α and PK15/IL32β injected groups. CD4^(+) T cells were increased in lymph node cell of PK15/IL32α and PK15/IL32β injected group and also, B cell population were increased in lymph node cell and spleen of PK15/IL18, PK15/IL32α and PK15/IL32β injected group. Therefore, we suggest that recombinant mouse IL-18 and recombinant human IL-32α,β suppress xenograft rejection in cellular xenotransplantation.
Park, Kwang-Il,Kang, Sang-Rim,Park, Hyeon-Soo,Lee, Do Hoon,Nagappan, Arulkumar,Kim, Jin A,Shin, Sung Chul,Kim, Eun Hee,Lee, Won Sup,Chung, Hyon-Jong,An, Su Jin,Kim, Gon Sup Hindawi Publishing Corporation 2012 Evidence-based Complementary and Alternative Medic Vol.2012 No.-
<P><I>Lonicera japonica THUNB.</I>, which abundantly contains polyphenols, has been used as a traditional medicine for thousands of years in East Asian countries because of the anti-inflammation properties. This study aimed to investigate the anti-inflammatory mechanism of polyphenol components isolated from Korea <I>L. japonica T.</I> by nuclear factor-kappaB (NF-<I><I>κ</I></I>B) and mitogen-activated protein kinases (MAPKs) pathway. Polyphenols significantly decreased lipopolysaccharide- (LPS-) induced mRNA and protein expression of inducible nitric oxide synthase and cyclooxygenase-2, as well as mRNA expression of tumor necrosis factor-alpha, interleukin- (IL-) 1<I><I>β</I></I>, and IL-6. Moreover, polyphenols inhibited nuclear translocation of NF-<I><I>κ</I></I>B p65, phosphorylation/degradation of the inhibitor of <I><I>κ</I></I>B, and phosphorylation of p38 MAPK, whereas the extracellular signal-regulated kinase and Janus N-terminal kinase were not affected. These results indicate that polyphenol components isolated from Korea <I>L. japonica T.</I> should have anti-inflammatory effect on LPS-stimulated RAW 264.7 cells through the decrease of proinflammatory mediators expression by suppressing NF-<I><I>κ</I></I>B and p38 MAPK activity.</P>
( Byoung Ok Cho ),( Jae Young Shin ),( Ji-su Kim ),( Denis Nchang Che ),( Hyun Ju Kang ),( Do-youn Jeong ),( Seon Il Jang ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.5
The present study was conducted with the aim to investigate the ameliorative effects of a new soybean product (cheonggukjang) fermented with Bacillus amyloliquefaciens SCGB1 (SFBA) in atopic dermatitis (AD) mouse model. Visual evaluation of AD induction in the mice indicated the remarkable control of SFBA in reducing the pathological severity of AD-like skin lesions reported as the SCORAD score of AD clinical symptoms. The results revealed that SFBA reduced dorsal skin and epidermal thickness to a similar extent with prednisolone. Further analysis revealed the dominance of SFBA in restraining mast cell infiltration in the dermis; immunoglobulin-E expression in serum; and TH2 IL-4 cytokine and itch-related IL-31 cytokine in the mice skin and serum. SFBA also suppressed scratching behaviours in mice induced by compound 48/80. Further histological findings also revealed the alleviation of collagen fiber deposition in dermal skin of the AD mice model. These actions of SFBA were examined to be mediated by its suppression of the phosphorylation activation of key signalling molecules such as NF-κB and MAPK responsible for the induction of cytokine production. Thus, SFBA can be considered as a promising functional food for managing clinical, histological and immunological spectra associated with AD.
Tris(tetraethylammonium) bistrimetaphosphate의 空間群 P1 ̄와 C2/c의 結晶學的 資料間의 相互變換
徐日煥,李珍昊,林星秀,朴晶蘭,秋錦洪,郭相姬,都暎圭,李海정 충남대학교 기초과학연구소 1994 忠南科學硏究誌 Vol.21 No.1
Tris(tetraethylammonium) bistrimetaphosphate, VP_6N_3O_18C_24H_60 의 結晶構造가 두가지 空間群 P1 ̄와 C2/c 로 밝혀졌다. 本 論文에서는 이 두가지 空間群의 結晶學的 資料間에 相互變換 關係가 成立함을 보였다. The structure of Tris(tetraethylammonium) bistrimetaphosphate, VP_6N_3O_18C_24H_60, has been elucidate with two space groups P1 ̄ and C2/c. In this paper, it has been shown that the crystallographic data of the two space groups are mutually transformed.
수온 변동 자극이 양식 넙치, Paralichthys olivaceus의 비특이적 생체 방어에 미치는 영향
이덕찬,김도형,김수미,강명석,홍미주,김현정,박수일 한국어병학회 2002 한국어병학회지 Vol.15 No.2
본 연구는 넙치에 대한 수온 변동 자극의 영향을 생체 방어적인 측면에서 검토하고자 하였다. 수온 변동 자극은 매일 18℃에서 25℃까지 상승과 하강을 반복하는 방법을 사용하였으며 그 기간은 30일간 지속하였다. 실험어는 온도변화를 주기 시작한 후 28일째까지 무작위로 채포하여 말초혈액 중의 백혈구 수, 전혈의 식작용능, 활성산소, 환원능, chemiluminescence(CL) response 및 lysozyme용균능과 같은 각종 비특이적 생체방어와 관련한 실험을 수행하였다. 수온 변동 자극을 주기 시작한 후 1일째부터 시작하여 1주일 이내에 약 18%의 넙치가 폐사하였으며 이후에는 대조구와 유사하였다. 또한, 수온 변동 자극을 준 실험구의 호중구 수는 2주째에 유의적인 증가를 보였으며, 림프구 수는 2일과 3일째에 감소하였으나 1주째부터 대조구와 유사한 수준으로 회복되었다. 실험구에서는 식작용 결과 식균율과 식균지수에서 이물질 투여 후의 반응이 늦게 나타났다. 식세포의 활성을 조사한 NBT reduction 실험에서는 실험 기간 동안 대조구와 유사한 경향ㅇ르 나타내었다. 또한, CL response의 경우, 실험구는 온도 변동 자극초기에 대조구에 비하여 유의적으로 낮았다. 그리고, 식세포가 분비하는 용균성 효소인 점액 내 lysozyme의 활성은 실험 기간 동안 실험구에서 낮게 나타나는 경향을 보였다. 이러한 여러 가지 비특이적 생체 방어 반응의 저하 현상은 수온 변동 자극 초기에 나타났으며, 1주일 정도 지속하면 그 환경에 적응하는 것을 알 수 있었다. 그리고 이러한 현상은 이들이 초기 폐사 발생과 어떤 상관 관계에 있는 것으로 생각되었다. This study was performed to know the effects of stress nduced by the daily fluctuation of water temperature fro 18? to 25? up and down for 30 days on the defence mechanism of olive flounde,r Paralichthys olivaceus. To make clear the temperature stress on the defense mechanism of the tested fish, several factors of immune response such as counting of leucocyte appearance in periopheral blood, phagocytic activity in whole blood cells, nitroblue tetrazolium(NBT) reduction, chemiluminescence(CL) response, and lysozyme activity were investigated at 28 days after giving the change of water temperature. The fish was controlled under the none feeding condition during experimental period. Mortality of the tested fish was rapidly increased up to 22% within the first one week of the experimental period without any additional stress factors. The number of neutrophil of peripheral blood in the tested group was significantly higher than the control group at the 2nd week, but the number of lymphocyte was significantly lower than the control group at the 1st and 3rd day of the experimental period, respectively. In the NBT reduction test, the activity of macrophage in the control group fish was the highest on the 7th day while that in the tested group was on the 3rd day. Also, the phagocytosis of tested group against formalin killed cells was retarded compared with the control CL response of the tested group was significantly lower from 2nd to 5th day of the experimental period than the contro. The lysozyme activity of tested group was remained higher during the experimental period than the contro. Even through the tested fish showed different results in some non-specific factors of immune responses between tested and control group fish, olive flounder seems highly adaptable in repeated water temperature change in condition after one week under the given temperature fluctuation range
Do, Su-Il,Kim, Kyung-Sook,Kim, Hee-Nam,Kim, Yeon-Jeong,Lee, Ki-Young,Kim, Bang-Ul,Kim, Cheorl-Ho,Lee, Young-Choon 東亞大學校附設遺傳工學硏究所 1999 遺傳工學硏究 Vol.- No.6
The cDNA encoding human Sia-α2,3-Gal-β1,4-Glc-NAc-R:α2,8-sialyltransferase, hST8Sia III, was isolated by screening of a human brain cDNA library with polymerase chain reaction-amplified DNA probe generated from the sequence of mouse ST8Sia III (mST8Sia III) and by 5' rapid amplification of cDNA ends of mRNA isolated from human brain tissues. Comparative analysis of the predicted protein-coding region between our cloned hST8Sia III and mST8Sia III showed 92 and 96% identities in the nucleotide and the amino acid sequence, respectively. The soluble hST8Sia III protein expressed in COS-7 showed an extremely high catalytic activity of transferring sialic acid through α2,8-linkage to intact fetuin glycoprotein, whereas the transferring activity was completely undetectable toward either α2,6-sialylated glycoprotein or desialylated glycoprotein acceptors. Northern analysis of hST8Sia III showed that the transcript corresponding to 11 kb was expressed in both human fetal and adult brain, while the expression of the 5.5-kb transcript was restricted to fetal liver, indicating that the expression of hST8Sia III is developmentally and tissue-specifically regulated.
Evaluation for Efficacies of Commercial Sanitizers and Disinfectants against Bacillus cereus Strains
Il Jin Kim,Ji-Hyoung Ha,Yong-Su Kim,Hyung-Il Kim,Hyun-Chul Choi,Dea-Hoon Jeon,Young-Ja Lee,Ae Jung Kim,Dong-Ho Bae,Keun-Sung Kim,Chan Lee,Sang-Do Ha 한국식품과학회 2009 Food Science and Biotechnology Vol.18 No.2
Bactericidal efficacies of various sanitizers and disinfectants against 10 Bacillus cereus strains isolated from Korean foods and 8 standard B. cereus strains were investigated. The sanitizing capabilities of ethanol, iodine, chloride, quaternary ammonium, hydrogen peroxide, and peroxide acetic acid were investigated using the EN 1276 method based on quantitative suspension testing. The resistance against sanitizers and disinfectants was higher for wild-type than standard strains, and the bactericidal activities decreased in dirty conditions. Ethanol, chlorine, and iodine at the maximum level allowed under Korean food sanitation laws showed a great effectiveness against B. cereus. Hydrogen peroxide at 1,100 ppm showed the lowest bactericidal activity against B. cereus. These results indicate that the legally allowed maximum concentrations of sanitizers and disinfectants in Korea do not reduce all B. cereus strains by at least 5 log₁? CFU/㎖.