이상환경조건에 따른 水稻의 物質生産 및 光合成作用의 調節과 그 機作에 관한 生態生理學的硏究 : 第1報 遮光處理가 水稻의 光合性速度와 그 관련형질 및 작물체형질에 미치는 영향

李忠烈 密陽産業大學校 農業技術開發硏究所 1998 農業技術開發硏究所報 Vol.2 No.1

차광처리가 수도의 형태적형질과 광합성속도 및 그 관련형질에 미치는 영향을 조사하기 위해. 1/5000a와그너 폿트에 1주씩 화영벼를 재배한후, 출수기이후에 56%로 차장처리를 15일간 실시한 후에 조사하였다. 차광처리에 의해 SLA는 모든 엽위에서 증가하였고 하위 엽보다 지엽에서 그 증가가 현저하였으며, 잎의 표면적도 처리구에서 널은 경향이었다. 또한 엽록소 함량은 차광에 의해 모든 잎에서 처리구가 증가하는 경향이었고, 무처리구에 있어서는 8월28일보다 9월11일에서 감소하는 경향이었다. 광합성속도는 모든 잎에서 광 강도가 강할 수록 증가하는 경향이었고, 차광 처리에 의해 무처리에 비해 증가하는 경향이었다. The rice was planted in 1/5000 a wagner pot to investigate the after effect of shading (56% of sun light) on the characteristics of plant, its related traits and photosynthetic activity at heading stage. The SLA(specific leaf area) and surface leaves were increased and more at flag leaves than lower leaves by the shading treatment. The shaded leaves of plant had a higher value in SPAD as compared with non-shaded leaves of plant. Shading at the heading stage increased photosynthetic rates of leaves Positioned on each part of the stem as compared with the non-shaded plants.

호르몬 불응성 전립선암 환자에서 mitoxantrone과 prednisone의 병합화학요법 후 통증완화 및 삶의 질 측정

이홍우,이지열,김세웅,이충범,강성학,조용현,황태곤,박용현,윤문수 대한화학요법학회 2000 대한화학요법학회지 Vol.18 No.3

목적 : 호르몬 불응성 전립선암 환자의 생명을 연장시키는 치료법은 현재로는 없으며, 현 시점에서의 치료목표는 증상의 경감 및 소실에 따른 삶의 질 향상에 두고 있다. 호르몬 불응성 전립선암의 고식적인 치료에 일차적인 치료법으로 간주되는 mitoxantrone과 prednisone의 화학요법이 호르몬 불응성 전립선암 환자의 통증완화 및 삶의 질에 어떠한 영향을 미치는지 알아보았다. 방법 : 1998년 7월부터 1999년 12월까지 증상이 있는 18명의 호르몬 불응성 전립선암 환자를 대상으로 하였으며, 일차적인 치료의 목표는 환자 본인이 기록하는 McGill-Melzack 6단계 통증척도에서 진통제의 증량 없이 2점이 감소(또는 첫 점수가 1점일 경우 통증의 완전소실)하는 것으로 하였다. 이러한 기준은 최소 3주 간격으로 시행한 자가 기록조사에서 연속적으로 2번 이상 유지될 때 유효한 것으로 간주하였다. 건강과 관련된 삶의 질 측정은 전신적 치료의 임상적 응용을 위한 실제적인 측정방법으로 개발되어 환자가 직접 작성하는 전립선암 특이성 삶의 질 측정기구(Prostate Cancer-Specific Quality-of-Life Instrument : PROSQOLI)의 비례연속형의 자가측정 척도(linear analogue self-assessment scale : LASA)를 이용하였으며, ECOG performance status를 사용하여 전신상태의 변화도 측정하였다. 결과 : 통증의 경감으로 나타나는 고식적인 치료의 반응은 66.7%(12/18)에서 나타났으며, 반응의 지속기간은 2개월에서 10개월까지로 평균 4.2±3.6개월이었다. PROSQOLI로 측정한 삶의 질은 통증에 대한 고식적인 치료반응을 보인 12명(66.7%) 모두가 삶의 질이 개선되었으며 특히 통증, 변비, 소변보기, 전반적인 복지상태에서의 유의한(p<0.05) 증가를 보였다. 치료 후 혈장 PSA 수치는 고식적인 반응의 유무에 관계없이 별 다른 변화를 보이지 않았으며, 골주사 또는 전산화 단층촬영으로 측정한 방사선학적 병변은 고식적인 반응을 보인 2명을 제외하고 치료 전과 비교하여 차이를 보이지 않았다. 병합 화학요법과 관련된 특별한 부작용은 관찰되지 않았다. 결론 : 통증을 동반한 호르몬 불응성 전립선암에서 mitoxantrone과 prednisone의 병합화학요법은 66.7%의 환자에서 통증의 감소 및 삶의 질이 향상되었다. 현재는 혈장 PSA 수치, 병소의 크기나 개수등이 치료의 지침이나 치료결과의 판정을 위한 객관적인 기준으로 사용되는 경향이 있지만, 앞으로는 환자를 중심으로 통증을 포함한 삶의 질 측정이 고식적 치료 후 치료결과 판정에 있어 객관적이고 적절한 방법이 될 것이다. Background : There is no evidence that therapeutic modalities prolong the survival of patients with hormone-refractory prostate cancer nowadays. The main goal of treatment is therefore improvement in quality-of-life including palliation of symptoms. We performed this study to investigate the benefit of mitoxantrone and prednisone chemotherapy in patients with symptomatic hormone-refractory prostate cancer using relevant end points of palliation regarding to pain and quality-of-life. Methods : From July 1998 to December 1999, we assessed the 18 patients with symptomatic hormone-refractory prostate cancer receiving mitoxantrone and low dose prednisone. The treatment end point was a palliative response defined as a 2-point decrease in pain assessed by a 6-point pain scale complemented by patients (or complete loss of pain if initially 1+) without an increase in analgesic medication and maintained for two consecutive evaluations at least 3 weeks apart. Health-related quality-of-life was evaluated with a series of linear analogue self-assessment(LASA) scale of the Prostate Cancer-Specific Quality-of-Life Instrument (PROSQOLI) and performance status was also measured. Results : Palliative responses were observed in 12(66.7%) of 18 patients. The duration of palliative responses were 4.2±3.6 months(range: 2 to 10 months). Treatment was well tolerated without specific side effects. There were no differences in prostate-specific antigen (PSA) level following treatment and no significant changes of radiologic findings evaluated by bone scan and/or CT were noted except 2 cases after chemotherapy. All the responding patients had an improvement in quality-of-life scales and performance status, however serum PSA levels were not changed. Conclusions : Chemotherapy with mitoxantrone and prednisone provides palliation and an improvement in quality-of-life for more than half (66.7%) of the patients with symptomatic hormone-refractory prostate cancer.

洛東江 流域의 土壤 特色 및 土地 利用 實態 調査

이충열,김용철,박현철,김성만,최인수 密陽産業大學校 農業技術開發硏究所 1998 農業技術開發硏究所報 Vol.2 No.1

1980년에 발표한 자료와 그 후 토양조사를 한 결과를 종합하여 낙동강 유역의 토양을 중심으로 우리 나라의 토양특성을 비롯한 각종 토양해설 자료를 근거로 요약하면 다음과 같다. 우리 나라 지형은 매우 복잡하나 산악이 대부분을 차지하고 있으며, 태백산을 중심으로 동부는 급경사의 지형이 발달하여 있고, 서부는 완만한 경사를 이루는 산악지가 많을 뿐만 아니라 우리 나라의 곡창지대라 할 수 있는 평야지는 한강을 비롯하여 낙동강, 금강, 및 영산강 유역에 분포하고 있다. 산악지의 면적이 많아 농경지의 절반 이상이 경사지에 분포하고 있는 것이 특징이라 할 수 있다. 토양 모재는 산성암 조립질인 화강암 또는 화강편마암이 전국의 2/3 이상 점유하고 있으며 수성암인 혈암 및 사암은 낙동강 유역이 위치한 경상남북도에 많이 분포하고 있다. 석회암은 강원도에 가장 많이 분포하고 있고 경기 및 경북의 북부지방에 약간 분포하며 기타 지역에는 산재하여 분포하고 있다. 이외에도 반암, 안산암, 유문암 및 편암 등이 분포하고 있으며 충적층은 주로 낙동강 유역을 포함한 4대강 및 이들 4대강의 재류에 넓게 분포하고 있다. 정밀 토양조사 결과 면적은 9,577,000ha(논 879,000ha, 밭 1,288,000ha, 과수 및 뽕밭 119,000ha, 초지 103,000ha, 임지 6,425,000ha, 기타 763,000ha), 이 중 낙동강 유역은 논, 밭을 포함한 농경지 240,731ha, 임지 1,132,655ha 및 기타 154,945ha로서 총 면적은 대략 1,565,332ha이다. The summary of the survey, which was based on the published data since 1980, on soil characteristics and land utilization of Korea including the basin of the Nakdong river is as follows : The mountainous area comprises the various physiographic features. The eastern part from Taebaeg Mountain is mostly covered by the steep sloped land and the western part perches on gently sloped hil1y area in which the major productive area is distributed along the alluvial plains of the major river basins, Han river, Nakdong river, Kum river and Youngsan river in Korea. More than half of the cultivated lands occur on the local valleys and mountain foot slopes. Soils from coarse textured acidic crystalline rocks such as granite and granitic gneiss cover more than 2/3 of the country. Soils from sedimentary rocks like shale and sandstone are mainly confined Kyungbuk province, soils from limestone are mainly in Kangwon and partly in Kyungki, Kyungbuk and other provinces, and others are from porphyry, andesite, gabbro, rhyolite and schist. Alluvial soils are mainly developed on four river basins and their tributaries. The area of whole cultivated land covered by the detailed soil survey was turned out to be 9.577.000ha, upland 879.000ha, paddy land 1,288,000ha, orchard and mulberry 119.000ha, grass land 103,000ha, forest 6,425,000ha and the others 763,000ha, In Nakdong river valley, total area was approximately 1,565,332ha and the cultivated area including upland and paddy land was 240,731ha. The area of forest and the others were respectively 1,132,655ha and 154,945ha.

사료용 교잡종 옥수수 연구 : Ⅲ. CNU와 SK계통을 이용한 교잡종 옥수수의 건물중 및 종실 수량

이희봉,김동욱,김준표,김용일,정재영,최현구,문현귀,이충열 충남대학교 농업과학연구소 2003 농업과학연구 Vol.30 No.2

공시 교잡종에 대한 각 지역에서 선발된 우수 교잡종의 간장 및 착수고는 대조구와 비슷한 경향이었으며, 생체 및 건물수량은 분얼수 및 간장과 관련이 큰 것으로 나타났으나 재식 밀도와 기상상태와도 밀접한 관계로 나타났다. 따라서 생체중은 대전지역에서 재배된 교잡종 H67이 대조구인 광안옥에 비해 53.2%로 높았고, 수원에서 역시 41.8%으로 높았으며, 종실수량은 H15과 H67에서 대조구보다 11.7%와 9.7%로 각각 높게 나타났다. 대전에서 분얼성인 H42와 무얼성인 H65에서 대조구보다 9.1%, 5.2%증가하였고, 수원에서는 분얼성이 H42와 무얼성인 H65에서 대조구보다 9.1%, 8.5% 증가하여 교잡종간, 지역간에 큰 차이를 보였다. 한편 후기 녹체성은 무얼형보다 분얼형에서 낮은 경향을 보였는데 SK계통과 교배된 조합에서 우수한 편으로 나타나 우수 교잡종 육성시 중요 고려사항으로 지적되었다. In order to develop the crude forage maize hybrids with high dry matter and grain yield, several hybrids evaluated as superior hybrids at three regions in 2001. They were planted and surveyed again for the second selection in 2002. The selected hybrids showed large difference in some major characteristics according to planting areas and dates(Suwon : April 25. Daejeon : May 15 and Milyang : May 25), but insect and disease injury were similar to check. Fresh and dry weight of the H67 hybrid at Suwon and Daejeon was the highest among hybrids including check, but low in Milyang due to raining after flowering. Grain yield was higher than check in H15 at Suwon H45 and H37 at Daejeon and H67 at Milyang.

형상인식을 적용한 용접 결함의 특징변수 추출

김재열,마상동,오성민,송경석,김양중,김창현,곽이구 朝鮮大學校 機械技術硏究所 1999 機械技術硏究 Vol.2 No.2

In this study, the researches classifying the natural flaws in welding parts are performed using the signal pattern classification method. For this purpose 500MHz storage digital oscilloscope including FFT function and enveloped waveform generator are used and the signal pattern recognition procedure is made up the digital signal processing, feature extraction, feature selection and classifier design. Specially it is composed with and discussed using the distance classifier is based in euclidean distance the empirical Bayesian classifier. In feature extracting, it is performed using the class-mean scatter criteria. And the signal Pattern classification method is applied to the signal pattern recognition problems of natural flaw that is porosity inclusion, slag inclusion, lack of penetration, lack of fusion. center crack, side crack, toe crack. root crack as the planar and volumetric flaw classification problem. According to this results, if appropriately learned the neural network classifier is better acquire the recognition rate of 86.31% above through it is different a little according to domain extracting the feature and the classifier

팥의 RAPD 분석 조건 최적화 연구

이충열,박현철,박진철,김성만,김용철,최재희,최인수 밀양대학교 농업기술개발연구소 2000 農業技術開發硏究所報 Vol.4 No.1

The object of this study was to optimize PCR condition for RAPD analysis in adzuki bean. The best template DNA concentration was 20ng(0.25unit taq polymerase and 2.5mM MgCl2), 40ng(1unit taq polymerase and 2.5mM MgCl2, 1unit taq polymerase and 4.5mM MgCl2 and lunit taq polymerase and 7.0mM MgCl2), and 60ng(0.5unit taq polymerase and 2.5mM MgCl2, 0.5unit taq polymerase and 4.5mM MgCl2, 1unit taq polymerase and 2.5mM MgCl2, and 1unit taq polymerase and 4.5mM MgCl2) The best MgCl2 concentration was 2.5mM(40ng template DNA and 1unit taq polymerase, 60ng template DNA and 0.5unit taq polymerase, and 60ng template DNA and 1unit taq polymerase), 4.5mM(20ng template DNA and 0.25unit taq polymerase, 60ng template DNA and 0.5unit taq polymerase, and 60ng template DNA and 1unit taq polymerase), and 7.0mM(40ng template DNA and 1unit taq polymerase). Amount of taq polymerase was 0.25unit(20ng template DNA and 2.5mM MgCl2), 0.5unit(60ng template DNA and 2.5mM MgCl2, 60ng template DNA and 4.5mM MgCl2) and 1unit(40ng template DNA and 2.5mM MgCl2, 40ng template DNA and 4.5mM MgCl2, 40ng template DNA and 7.0mM MgCl2, 60ng template DNA and 2.5mM MgCl2, 60ng template DNA and 4.5mM MgCl2, and 60ng template DNA and 7.0mM MgCl2). When we consider results from template DNA concentration, MgCl2 concentration, and amount of taq polymerase, the best condition for PCR optimization was 60ng template DNA, 4.5mM MgCl2, and 1unit taq polymerase. Reaction temperatures for the optimal PCR condition were 84℃, 32℃, 62℃; 90℃, 40℃, 72℃; and 92℃, 36℃, 72℃.

작약의 RAPD 분석을 위한 PCR 최적조건 구명

최인수,김성만,김용철,이충렬,박현철 밀양대학교 농업기술개발연구소 2000 農業技術開發硏究所報 Vol.4 No.1

To optimize the PCR condition is one of the most important steps for RAPD analysis. The purpose of this study was to optimize PCR condition in Paeonia. 3×3×3 factorial experiment for template DNA concentration, MgCl2 concentration, and amount of taq polymerase was conducted. Another factorial experiment for reaction temperature(denature, annealing, and extension) was also conducted. The most appropriate template DNA concentration was 60ng. Clear bands were observed from 2.5mM and 4.5mM of MgCl2 if template DNA concentration and amount of taq polymerase were proper. Amount of taq polymerase for the optimal PCR condition was 0.5unit and 1unit. In the consideration of results from template DNA concentration, MgCl2 concentration, and amount of taq polymerase, 4 conditions (60ng of template DNA, 2.5mM MgCl2 and 0.5unit taq polymerase; 60ng of template DNA, 4.5mM MgCl2 and 0.5unit taq polymerase; 60ng of template DNA, 4.5mM MgCl2 and lunit taq polymerase; and 40ng of template DNA, 4.5mM MgCl2 and 1unit taq polymerase) were best combinations for the optimal PCR condition. Reaction temperature for the optimal PCR condition was 92℃, 36℃, 72℃.

박의 RAPD 분석을 위한 PCR 최적 조건 연구

김보경,김성만,김용철,이충열,박현철,최인수 밀양대학교 농업기술개발연구소 2000 農業技術開發硏究所報 Vol.4 No.1

This study was conducted to identify the best combinations of factors(template DNA concentration, MgCl2 concentration, and amount of taq polymerase) for the optimization of PCR in gourd. 3×3×3 factorial experiment for template DNA concentration, MgCl2 concentration, and amount of taq polymerase was conducted. Another factorial experiment for denature, annealing, extension temperature on the optimal PCR condition was also conducted. The most clear and reproducible bands were appeared in 40ng(1unit taq polymerase and 2.5mM MgCl2, and lunit taq polymerase and 4.5mM MgCl2, 1unit taq polymerase and 7.0mM MgCl2), 60ng(0.5unit taq polymerase and 2.5mM MgCl2, 0.5unit taq polymerase and 4.5mM MgCl2, 1unit taq polymerase and 2.5mM MgCl2, 1unit taq polymerase and 4.5mM MgCl2, and 1unit taq polymerase and 7.0mM MgCl2). The best MgCl2 concentration was 4.5mM(40ng template DNA and 1unit taq polymerase, 60ng template DNA and 0.5unit taq polymerase, and 60ng template DNA and lunit taq polymerase), and 7.0mM(40ng template DNA and 0.5unit taq polymerase DNA). The combinations which showed the best bands from the amount of taq polymerase were 0.5unit(60ng template DNA and 4.5mM MgCl2) and lunit(40ng template DNA and 2.5mM MgCl2, 40ng template DNA and 4.5mM MgCl2, 40ng template DNA and 7.0mM MgCl2, 60ng template DNA and 2.5mM MgCl2, 60ng template DNA and 4.5mM MgCl2, 60ng template DNA and 7.0mM MgCl2). When we consider results from template DNA concentration, MgCl2 concentration, and amount of taq polymerase, 1 condition(40ng of template DNA, 4.5mM MgCl2 and 1unit taq polymerase) was the best combination for the optimal PCR condition. Reaction temperatures for the optimal PCR condition were 84℃, 32℃, 62℃ and 86℃, 35℃, 64℃.

土壤水分別 播種期와 窒素施肥方法이 율무의 光合成과 生育 및 엽고병에 미치는 影響

金容撤,李忠烈,崔仁洙,朴賢哲,金成萬,林銖三 밀양대학교 농업기술개발연구소 2000 農業技術開發硏究所報 Vol.4 No.1

In order to establish optimal seeding date and nitrogen fertilization of adlay in barley-dlay cropping system of south-eastern part of Korea, yield and its components of adlay grown under at 3 different seeding dates and 2 nitrogen fertilizations were compared and analyzed. In addition, edible nutriants of adlay grain were compared with rice and woheat, between upland and paddy cultivation, and among three cultivars. The results obtained are summarized as follow: 1.Heading date and maturity date was fasted later seeding date than early seeding. Days to heading was decreased as the seeding date was delayed. 2.Stem leagth, stem diameter and No. of tiller were increased irrigation than control(non-irrigation), and was decreased as the seeding date was delayed. 3.Top and root dry weight were increased moisture were decreased irrigation than control(non-irrigation), and was decreased as the seeding date was delayed. 4.Net photosynthetic rate was increased at treatment of irrigation. Key word : adlay, seeding daye, soil water content, photosynthesis, growth.

光 强度別 아주까리의 光合成速度와 氣孔傳導度의 變化 및 相互聯關性

韓英熙,崔仁洙,朴賢哲,金成萬,金容澈,李忠烈 밀양대학교 농업기술개발연구소 2000 農業技術開發硏究所報 Vol.4 No.1

The Ricinus communis L. was planted 70cm x 70cm in field to investigate on the changes of photosynthesis followed by the investigation on the light intensity. The photosynthetic rate and stomatal conductance were increased as the PAR was increased to 2000μ mol/m2/s in leaves of all. A linear equation were obtained between net photosynthetic and transpiration rate. The quadratic equations were obtained between net photosynthetic rate and stomatal conductance, and a linear equation was obtained between transpiration rate and stomatal conductance. It was also confimed that intercellular CO2 concentration was significantly correlated with net-photosynthetic rate and stomatal conductance under low light intensity.