간 허혈성괴상의 CT소견에 대한 실험적 연구 : 간실질 및 피막의 경시적 변화와 병리조직의 비교 Changes of CT Findings of Liver Parenchyama and Capsule with Time Lapse Correlated with Histopathology

김창근,김강득,최시성,정선관,노병석,원종진 圓光大學校 醫科學硏究所 1994 圓光醫科學 Vol.10 No.1-2

In order to evaluate CT findings with time and to confirm the capsular structure correlating with its histopathologic findings, we experimentally induced ischemic necrosis of liver in 20 rabbits. Incision of the abdomen was performed under anesthesia. Ligation of hepatic artery, portal vein and hepatic duct of right inferior posterior lobe of the liver after injection of macroaggregated albumin into right inferior posterior portal vein in order to prevent from developing collateral circulation as possible. Precontrast and postcontrast enhanced CT images were obtained at 1. 2. 4 days and 1, 2. 4. 6 weeks after induction of ischemic necrosis. Necrotic area in the liver showed homogeneous low density compared to normal liver, and no contrast enhancement after contrast material administration, and marked volume loss with time. CT attenuation value of necrotic area at 1 day(36.7±6.4 M±SD, Hounsfield unit) approached to two third of that of normal liver(56.3±7.3) and showed no significant change until two weeks but increased significantly after four weeks. On contrast enhanced CT images, capsular structure was obsereved as a high density rim along the liver surface of the necortic area in 8 of 15 cases. From the first day on, homogenous ischemic or coagulation necrosis was observed but rarely findings of edema, hemorrhage and cystic change were observed on histopathologic specimen. In all of the histologic samples taken from 10 cases during th 2nd to 6th week, fibrous thickening with rich vascularity was observed along the surface of the necrotic area. In conclusion, ischemic necrosis of the liver showed homogeneous low density and no contrast enhancement. CT attenuation value of necrotic area increased significantly with time associated with markedly decrease in volume. Capsular structure attributed to the hypertrophy of the liver capsule and rich vascularity within it.

흰쥐에서 Iodine-131-Iododeoxyadenosine 의 생체분포 및 유방암 영상화에 관한 연구

김혜원,원종진,최시성,이현철,김창근,김선구,Yang, David J,이강무,민병철,이종덕,Kim, E Edmund 대한핵의학회 1998 핵의학 분자영상 Vol.32 No.4

Purpose: I-131 labeled (2'-deoxy-2-iodo-β-D-arabinofuranosyl) adenine (IAD) may be involved in DNA synthesis during active proliferation of tumor cells. We conducted this study to find out the biodistribution of IAD and its feasibility for scintigraphic tumor imaging. Materisls and Methods: Tosyl acetyl-adenosine was dissolved in acetonitrile, and I-131-NaI was added and heated to synthesize IAD. Female Fisher 344 rats innoculated with breast tumor cells were injected witb 0.27 MBq of IAD. Rats were sacrificed at 0.5, 1, 2, 4, 24h and the % of injected dose per gram of tissue (%ID/g) was determined. For scintigraphy, rats bearing breast cancer were administered with 1.11 MBq of IAD and imaging was perforrned after 2 and 24h. Then, rat body was fixed and rnicrotomized slice was placed on radiographic film for autoradiography, Results: %ID/g of tumor wa.' 0.74 (0.5h), 0.73 (1h), 0.55 (2h), 0.38 (4h), and 0.05 (24h), respectively. At 1h after injection, %ID/g of tumor was higher than that of heart (0.34), liver (0.42), spleen (0.47), kidney (0,69), muscle (0.14), bone (0.33) and intestine (0.51). However, %1D/g of tumor was lower than blood (1.06), lung (0.77), and thyroid (177.71). At 4h, %ID/g of tumor in comparison with other tissue did not change. Tumor contrast expressed by tumor to blood ratio was 0.69 and tumor to muscle ratio was 5.11 at 1h. However, these ratios did not improve through 24h. On autoradiogram and scintigraphy at 2 and 24 hour, the tumor was well visualized. Conclusion: This results suggest that Ial) may have a potential for tumor scintigraphy. However, further work is needed to improve localization in tumor tissue. (Korean J Nucl Med 1998;32:374-81)

CT-26 선암을 접종한 마우스에서 Iodine-131-Iodomisonidazole 의 생체분포 및 종양저산소증의 영상화

Yang, David J,Kim, E Edmund,김혜원,김창근,윤권하,김현정,정선관,노병석,Lee, Hyun-Chul 대한핵의학회 1999 핵의학 분자영상 Vol.33 No.3

urpose: Misonidazole is a radiosensitizer that binds in hypoxic cells. The purpose of this study was to find out the feasibility of I-131-Iodomisonidazole (IMISO) for imaging of tumor hypoxia. Materials and Methods: Tosyl precursor was dissolved in acetonitrile and I-131-NaI was added to synthesize IMISO. Balb/c mice inoculated with CT-26 adenocarcinoma were injected with IMISO. Mice were sacrificed at 1,2,4,24 hr and % of injected dose per gram of tissue (%ID/g) was determined. For scintigraphy and MRI, mouse bearing CT-26 adenocarcinoma was administered with IMISO and imaging was performed 4 hr after. Then, mouse body was fixed and microtomized slice was placed on radiographic film for autoradiography. Results: %ID/g of tumor was 1.64 (1h), 0.98 (2h), 0.85 (4h) and 0.20 (24h), respectively. At 24h, %ID/g of tumor was higher than that of all other tissues except thyroid. Tumor to muscle ratio increased with time and tumor to blood ratio also increased with time and reached 1.53 at 24 hr. On autoradiogram, tumor was well visualized as an increased activity in central hypoxic area of the tumor which corresponds to the area of high signal intensity on T2-weighted MR image. On scintigraphy, tumor uptake was visualized. Conclusion: This results suggest that IMISO may have a potential for tumor hypoxia imaging in mouse model. However, further study is needed to improve it's localization in tumor tissue and to achieve acceptable images of tumor hypoxia.

Wegener 씨 육아종 증례보고 1예 : A Case Report

정선관,임형근,정은택,노병석,김창근,원종진 圓光大學校 醫科學硏究所 1995 圓光醫科學 Vol.11 No.2

Wegener's granulomatosis is characterized by necrotizing and granulomatous disease which involves upper, lower respiratory tract and kidney. The basic pathophysiologic mechanism of Wegener's granulomatosis is still unknown. We report a case of Wegener's granulomatosis which involved lung and pleura and was diagnosed by clinical symptoms, radiologic findings and anti-neutrophilic cytoplasmic autoantibodies.

골신티그라피에서 경험한 비정상적인 연조직 섭취(전이석회화, 횡문근융해증, 가성막결장염)

박순아,김창근,김대응 圓光大學校 醫科學硏究所 2006 圓光醫科學 Vol.21 No.2

담즙분비촉진 식물추출물을 이용한 심근관류영상 검사법 개선

정환정,김창근 대한핵의학회 2003 핵의학 분자영상 Vol.37 No.6

목적: 심근관류 영상은 관상동맥 질환을 진단하고 환자의 예후를 평가하는데 널리 사용되고 있다. ^(99m)Tc 제재를 이용한 심근관류 영상은 간 방사능을 줄이고 좋은 영상을 얻기 위해 주사 후 일정시간을 대기하고, 지방식을 섭취하는 검사방법을 사용하고 있다. 이번 연구에서는 식물 추출물 혼합제대를 이용하여 간 방사능의 배출을 촉진함으로써 검사시간을 단축시키고, 심근영상의 질을 향상시킬 수 있는 지 알아보고자 하였다. 대상 및 방법: 프로토콜 1은 식물추출물의 환약제대(이하 혼합제재)를 투여한 개선법이 간 방사능의 배출을 촉진하고 영상의 질을 향상시키는 지 알아보기 위해 건강하고 젊은 자원자 10명(남:녀=7: 3, 평균 연령 24.1±2.4세)을 대상으로 혼합제재를 복용하고 15분 후 MIBI를 주사하였으며, 주사 후 30분, 60분, 90분, 그리고 120분재 영상을 얻어 분석하였다. 프로토콜 2는 ^(99m)Tc 심근관류 영상법에서 널리 이용되고 있는 1일 휴식-부하방법에 혼합제재를 사용하였을 때의 개선법의 효과를 알아보기 위해 다른 자원자 10명(남: 녀=8: 3, 평균 연령 26.1±2.9세)을 대상으로 개선법과 고식적방법(우유와 아이스크림 등 지방식 투여)을 적용한 영상을 얻어 분석하였다. 결과: 프로토콜 1에서 개선법과 고식적 방법의 심근/폐 방사능 비는 90분 영상까지 유의한 차이를 보이지 않았으며, 간/폐 방사능 비는 120분 영상까지 개선법이 고식적 방법에 비해 유의하게 낮은 값을 보였다. 심근/간 방사능비는 개선법에서 지속적으로 높은 값을 보였다. 프로토콜 2에서 첫 번째 MIBI를 주사한 후 심근/폐 방사능은 유의한 차이가 없었으나 심근/간 방사능 비는 45분과 60분 영상에서 개선법이 유의하게 높은 값을 보였다. 두 번재 MIBI를 주사한 후에는 20분 영상부터 지속적으로 개선법의 심근/간 방사능 비가 높은 값을 보였으나, 첫 번째 주사와 마찬가지로 심근/폐 방사능 비는 차이가 없었다. 결론: 담즙 배출을 촉진하는 천연식물 추출 혼합제재를 심근관류 영성을 획득하는데 적용하면 지방식 섭취없이 검사 소요 시간을 2시간대로 단출시킬 수 있어 환자의 불편을 최소화시키면서 영상의 질을 향상시킬 수 있을 것으로 판단된다. Purpose: For good quality of myocardial perfusion images, an approximately 30 min to 1 hour of waiting time after radiopharmaceutical injection and ingestion of fatty meal are asked of the patients. The aim of this study was to investigate the shortening of waiting time after radiopharmaceutical injection and improvement of image quality using natural plant extracts that promote bile excretion. Materials and Methods: Ten volunteers participated in protocol 1 (7 men, 3 women; mean age, 24.1±2.4 years) and protocol 2 (8 men, 2 women; mean age, 26.1±2.9 years), respectively. For the modified method of both protocols, subjects took natural plant extracts 15 minutes before the first injection of ^(99m)Tc MIBI without taking fatty meals. Control (Conventional) methods were performed with intake of a fatty meal 20 to 30 minutes after ^(99m)Tc MIBI injection. Results: As the results of protocol 1 and 2, the ratio of myocardial to lung ratio were not different between modified and conventional method. Liver to lung ratio of modified method showed significantly lower value than that of conventional method. In modified method, myocardial to liver ratio was higher persistently. In protocol 2, natural plant extracts took before the first injection of ^(99m)Tc MIBI exerted accelerating effect of excretion of bile juice into intestine until the end of examination. Conclusion: These results represent that natural plant extracts for facilitation of bile excretion before injection of ^(99m)Tc MIBI may provide better quality of myocardial perfusion images without the need for preparations such as ingestion of fatty meal within the 2 hours compared with conventional method.

만성간질환에서 Tc-99m-phytate 동적간스캔을 이용한 간기능 지표의 분석

박순아,김대응,김창근 圓光大學校 醫科學硏究所 2006 圓光醫科學 Vol.21 No.2

비호지킨 림프종에서 경험한 변형된 Ga-67의 섭취 분포

장주아,박순아,김대응,김창근 圓光大學校 醫科學硏究所 2007 圓光醫科學 Vol.22 No.1

Scintigraphy를 이용한 척수 손상 환자와 정상인의 담즙 배설양상 비교

신용일,양충용,주민철,위훈,정환정,김창근,길은영 圓光大學校 醫科學硏究所 2004 圓光醫科學 Vol.19 No.1

유방암 환자에서 종양조직내 Collagenase 활성도와 골스캔과의 비교

김창근(Chang Guhn Kim),최시성(See Sung Choi),김현정(Hyun Jeong Kim),김선구(Seon Gu Kim),임형근(Hyung Guhn Lim),노병석(Byung Suk Roh) 대한핵의학회 1996 핵의학 분자영상 Vol.30 No.3

N/A Purpose: It has been known that the activity of extracellular matrix degradative enzymes such as collagenase correlate well with the metastaic potential of various tumor cells in experimental study. This study was aimed at comparing the activities of type IV collagenase with bone scan findings in patients with breast cancer. Materials and Methods: We retrospectively correlated bone scan findings with the results of immunohistochemical staining for 92kDa, 72kDa type IV collagenase in 28, and 30 patients with metastatic breast cancer, respectively, as well as 23, and 27 patients with primary breast cancer, respectively. The immunohistochemical staining was performed with tissue specimens obtained from primary or metastatic breast tumor lesions. The amounts of the enzyme were graded from 0 to 4 and scored by multiplication metastasis was also scored from 1 to 5 with increasing probability. Results: There was a significant difference in enzyme scores between patients with and without metastasis. In patients with primary breast cancer group, the frequency of patients with enzyme score of less than 170 were 96%(26/27) and 100%(26/26) with 92kDa and 72kDa collagenase, respectively. In contrast, in patients with metastatic breast cancer group, the frequency of patients with enzyme score of more than 200 were 93%(28/30) and 87%(26/30) with 92kDa and 72kDa collagenase, respectively. All patients with each enzyme score of less than 170 show no active bony metastasis, however, there were variable bone scan findings in patients with each enzyme score of more than 200. Conclusion: Bone scan is useful to confirm, localize or follow up of bony metastasis in patients with each enzyme scores of more than 200. Acitve metastatic lesions were hardly seen on the bone scintigraphy in patients with collagenase scores of less than 170.