Ion scattering spectroscopy study of the Si(001)c(4x4)-C surface reconstruction

Park J. Y.,Chae K. H.,Choi D. S.,Kim J. Y.,Kim S. S.,Seo J. H.,Whang C. N. 한국물리학회 2004 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.45 No.3

Reconstructed Si(001)c(4 4)-C surface has been studied by coaxial impact collision ion scattering spectroscopy (CAICISS). When 100L ethylene (C2H4) was exposed on Si(001)-(2 1) surface at 700 C, Si(001) dimer structures were changed by induced carbon (C) atoms. The experimental CAICISS spectra and simulation results reveal that the reconstructed Si(001)c(4 4)-C surface shows good agreement with the missing dimer model, rather than the Si-C heterodimer model, and adsorbed C atoms in uence only the reconstructed vertical plane of Si(001) surface. On comparing the azimuthal-scan curves for 100L C/Si(001) with those for clean Si(001), it can be suggested that C atoms occupy the fourth subsurface layer of Si(001) directly below the HB (bridge) site. These results are new evidence supporting the previous studies based on the C incorporation into Si(001) surface with missing dimers and the substitution of the fourth Si layers.

Characterization of Phosphoinositide-3-kinase, Class 3 (PIK3C3) Gene and Association Tests with Quantitative Traits in Pigs

Kim, J.H.,Choi, B.H.,Lim, H.T.,Park, E.W.,Lee, S.H.,Seo, B.Y.,Cho, I.C.,Lee, J.G.,Oh, S.J.,Jeon, J.T. Asian Australasian Association of Animal Productio 2005 Animal Bioscience Vol.18 No.12

This study deals with the characterization of porcine PIK3C3 and association tests with quantitative traits. PIK3C3 belongs to the class 3 PI3Ks that participate in the regulation of hepatic glucose output, glycogen synthase, and antilipolysis in typical insulin target cells such as those in the such as liver, muscle system, and fat. On the analysis of full-length mRNA sequence, the length of the PIK3C3 CDS was recorded as 2,664 bps. As well, nucleotide and amino acid identities between human and pig subjects were 92% and 99%, respectively. Five SNPs were detected over 5 exons. We performed genotyping by using a SNP C2604T on exon24 for 145 F$_2$ animals (from a cross between Korean native boars and Landrace sows) by PCR-RFLP analysis with Hpy8I used to investigate the relationship between growth and fat depot traits. In the total association analysis, which doesn' consider transmission disequilibrium, the SNP showed a significant effect (p<0.05) on body weight and carcass fat at 30 weeks of age as well as a highly significant effect (p<0.01) on back fat. In an additional sib-pair analysis, C allele still showed positive and significant effects (p<0.05) on back fat thickness and carcass fat. Moreover, the effects of C allele on the means of within-family components for carcass fat and back fat were estimated as 2.76 kg and 5.07 mm, respectively. As a result, the SNP of porcine PIK3C3 discovered in this study could be utilized as a possible genetic marker for the selection of pigs that possess low levels of back fat and carcass fat at the slaughter weight.

The effects of mesenchymal stem cells injected via different routes on modified IL-12-mediated antitumor activity

Seo, S H,Kim, K S,Park, S H,Suh, Y S,Kim, S J,Jeun, S-S,Sung, Y C Nature Publishing Group 2011 Gene Therapy Vol.18 No.5

<P>Owing to its tumor tropism and prolonged transgene expression, mesenchymal stem cell (MSC) has been considered as an ideal delivery vehicle for cancer gene therapies or therapeutic vaccines. In this study, we demonstrated that intratumoral (i.t.) injection of MSCs expressing modified interleukin-12 (MSCs/IL-12M) exhibited stronger tumor-specific T-cell responses and antitumor effects as well as more sustained expressions of IL-12 and interferon (IFN)-γ in both sera and tumor sites than did IL-12M-expressing adenovirus (rAd/IL-12M) in mice bearing both solid and metastatic tumors. Subcutaneous (s.c.) injection of MSCs/IL-12M at contralateral site of tumor exhibited similar levels of serum IL-12 and IFN-γ as i.t. injection, but much weaker antitumor effects in both B16F10 melanoma and TC-1 cervical cancer models than i.t. injection. Although intravenous (i.v.) injection elicited earlier peak serum levels of cytokines, it induced weaker tumor-specific T-cell responses and antitumor effects than i.t. injection, indicating that serum cytokine levels are not surrogate indicators of antitumor effects. Taken together, these results indicated that MSC is more efficient than adenovirus as a cytokine gene delivery vehicle and that i.t. injection of MSCs/IL-12M is the best approach to induce strong tumor-specific T-cell responses that correlate with anti-metastatic effects as well as inhibition of solid tumor growth, although MSCs themselves have an ability to migrate into the tumor site. In addition, MSCs/IL-12M embedded in Matrigel (MSCs/IL-12M/Matrigel) exhibited significant antitumor effects even in immunodeficient mice such as SCID and BNX mice lacking T, B and natural killer (NK) cells, but not in IFN-γ knockout mice. Our findings provide an optimal approach for designing an efficient clinical protocol of MSC-based cytokine gene therapy to induce strong tumor-specific T-cell responses and therapeutic anticancer efficacy.</P>

C3, C4 의 Nephelometric 측정 경험

조병철 ( B C Cho ),검덕희 ( D H Kim ),김민숙 ( M S Kim ),서덕규 ( D K Seo ) 대한임상검사과학회 1990 대한임상검사과학회지(KJCLS) Vol.22 No.1

RIA has been used to measure accurately a minute quantity of Ags, such as proteins. hormones, enzymes or drugs. However, due to the presence of a radication hazard and the increment of having to pool test specimens. RIA is gradually being replaced by other immunoassay that have a comparable sensitivity but do not require radiocative meterial. Turbidimetric immunoassay, latex immunoassay and fluorescence immunoassay are such examples. We at the department of clinical pathology, Asan medical center, compared CH 50 activity with C3 , C4 quantity by nephelometric method.

비주얼 C++을 이용한 재료역학 해석 프로그램 개발

이상순(S.S. Lee),서성삼(S.S. Seo),이승철(S.C. Lee),허윤(Y. Hur),정해영(H.Y. Jung) 대한기계학회 2001 대한기계학회 춘추학술대회 Vol.2001 No.3

The computer program as a supplemental tool for a mechanics of materials course has been developed using visual C++. A unique feature of this computer program is that it is suitable for engineering students at the sophomore/junior level and for students who are totally new to the computer programing. Solutions to problems are given in both graphical and numerical forms. This program provides a learning environment where students can focus on understanding and solving various problems rather than typing cumbersome expressions and checking numerical errors.

유전질환 등록 시스템 운영, 유전자 정보 Database 구축 및 유전자 진단 시스템 확립

구수경,이환석,손용규,김성수,이광수,김지인,서일선,진수정,정동기,오범석,정성철,이진성 국립보건연구원 2000 국립보건원보 Vol.37 No.-

Dielectric Properties of Highly Oriented Pb0.65Ba0.35ZrO3 Thin Films

J.S. Kim,J. S. Choi,J. H. Lee,S. H. Kim,S. H. Jeon,I. R. Hwang,B. H. Park,T.J. Choi,S.H. Shin,J.C. Lee,이명재,S. A. Seo,I. K. Yoo 한국물리학회 2005 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.47 No.2

Pb0.65Ba0.35ZrO3 (PBZ) thin films [1] were grown on MgO (001) substrates by pulsed laser deposition (PLD). We compared the structural and dielectric properties of PBZ thin films grown at various temperatures. A high c.axis orientation appeared in PBZ thin film grown at a deposition temperature of 550 C. The c.axis-oriented PBZ thin film also showed the largest tunability among all the PBZ thin films in capacitance-voltage measurements. The tunability and dielectric loss of the PBZ thin film were 20 % and 0.00959, respectively. In addition, we compared the temperature coefficient of capacitance (TCC) of a PBZ thin film with that of a Ba0.5Sr0.5TiO3 (BST) thin film, which is a well-known material applicable to tunable microwave devices. We confirmed that the TCC value of a PBZ thin film was three times smaller than that of a BST thin film. Pb0.65Ba0.35ZrO3 (PBZ) thin films [1] were grown on MgO (001) substrates by pulsed laser deposition (PLD). We compared the structural and dielectric properties of PBZ thin films grown at various temperatures. A high c.axis orientation appeared in PBZ thin film grown at a deposition temperature of 550 C. The c.axis-oriented PBZ thin film also showed the largest tunability among all the PBZ thin films in capacitance-voltage measurements. The tunability and dielectric loss of the PBZ thin film were 20 % and 0.00959, respectively. In addition, we compared the temperature coefficient of capacitance (TCC) of a PBZ thin film with that of a Ba0.5Sr0.5TiO3 (BST) thin film, which is a well-known material applicable to tunable microwave devices. We confirmed that the TCC value of a PBZ thin film was three times smaller than that of a BST thin film.

Intravesical Instillation of c-MYC Inhibitor KSI-3716 Suppresses Orthotopic Bladder Tumor Growth

Jeong, K.C.,Kim, K.T.,Seo, H.H.,Shin, S.P.,Ahn, K.O.,Ji, M.J.,Park, W.S.,Kim, I.H.,Lee, S.J.,Seo, H.K. Williams and Wilkins Co 2014 The Journal of urology Vol.191 No.2

Purpose: c-MYC is a promising target for cancer therapy but its use is restricted by unwanted, devastating side effects. We explored whether intravesical instillation of the c-MYC inhibitor KSI-3716 could suppress tumor growth in murine orthotopic bladder xenografts. Materials and Methods: The small molecule KSI-3716, which blocks c-MYC/MAX binding to target gene promoters, was used as an intravesical chemotherapy agent. KSI-3716 action was assessed by electrophoretic mobility shift assay, chromatin immunoprecipitation, transcription reporter assay and quantitative reverse transcriptase-polymerase chain reaction. Inhibition of cell proliferation and its mechanism was monitored by cell cytotoxicity assay, EdU incorporation assay and flow cytometry. The in vivo efficacy of KSI-3716 was examined by noninvasive luminescence imaging and histological analysis after intravesical instillation of KSI-3716 in murine orthotopic bladder xenografts. Results: KSI-3716 blocked c-MYC/MAX from forming a complex with target gene promoters. c-MYC mediated transcriptional activity was inhibited by KSI-3716 at concentrations as low as 1 μM. The expression of c-MYC target genes, such as cyclin D2, CDK4 and hTERT, was markedly decreased. KSI-3716 exerted cytotoxic effects on bladder cancer cells by inducing cell cycle arrest and apoptosis. Intravesical instillation of KSI-3716 at a dose of 5 mg/kg significantly suppressed tumor growth with minimal systemic toxicity. Conclusions: The c-MYC inhibitor KSI-3716 could be developed as an effective intravesical chemotherapy agent for bladder cancer.

High-resolution 3-μm spectra of Jupiter: Latitudinal spectral variations influenced by molecules, clouds, and haze

Kim, S.J.,Geballe, T.R.,Kim, J.H.,Jung, A.,Seo, H.J.,Minh, Y.C. Academic Press 2010 Icarus Vol.208 No.2

We present latitudinally-resolved high-resolution (R=37,000) pole-to-pole spectra of Jupiter in various narrow longitudinal ranges, in spectral intervals covering roughly half of the spectral range 2.86-3.53μm. We have analyzed the data with the aid of synthetic spectra generated from a model jovian atmosphere that included lines of CH<SUB>4</SUB>, CH<SUB>3</SUB>D, NH<SUB>3</SUB>, C<SUB>2</SUB>H<SUB>2</SUB>, C<SUB>2</SUB>H<SUB>6</SUB>, PH<SUB>3</SUB>, and HCN, as well as clouds and haze. Numerous spectral features of many of these molecular species are present and are individually identified for the first time, as are many lines of H<SUB>3</SUB><SUP>+</SUP> and a few unidentified spectral features. In both polar regions the 2.86-3.10-μm continuum is more than 10 times weaker than in spectra at lower latitudes, implying that in this wavelength range the single-scattering albedos of polar haze particles are very low. In contrast, the 3.24-3.53μm the weak polar and equatorial continua are of comparable intensity. We derive vertical distributions of NH<SUB>3</SUB>, C<SUB>2</SUB>H<SUB>2</SUB> and C<SUB>2</SUB>H<SUB>6</SUB>, and find that the mixing ratios of NH<SUB>3</SUB> and C<SUB>2</SUB>H<SUB>6</SUB> show little variation between equatorial and polar regions. However, the mixing ratios of C<SUB>2</SUB>H<SUB>2</SUB> in the northern and southern polar regions are ∼6 and ∼3 times, respectively, less than those in the equatorial regions. The derived mixing ratio curves of C<SUB>2</SUB>H<SUB>2</SUB> and C<SUB>2</SUB>H<SUB>6</SUB> extend up to the 10<SUP>-6</SUP> bar level, a significantly higher altitude than most previous results in the literature. Further ground-based observations covering other longitudes are needed to test if these mixing ratios are representative values for the equatorial and polar regions.

여호와 증인 환자에서 산부인과적 무혈 수술의 임상적 고찰

이성욱,오일영,서상기,반태학,박윤희,박성철,한광수 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.5

목적 : 여호와의 증인은 자신들의 교리에 따라 혈액이나 기타 혈액제재의 사용을 거부하는 종파이다. 이들에 대한 산부인과적 무혈 수술의 임상상 및 수술후 결과를 후향적으로 검토하였다. 연구방법 : 1995년 2월부터 1999년 8월까지 세종병원에서 입원 기간 중 어떠한 혈액이나 혈액제재를 투여 받지 않았던 여호와의 증인 환자 50예에 대해 무혈 수술의 성적을 후향적으로 조사하였다. 결과 : 환자의 연령 분포는 9세와 58세 사이로 평균 36.7 ± 8.7세 이었다. 무혈 수술의 가장 흔한 사례는 제왕 절개술이었다. 인간 조혈촉진인자(EPO)를 제왕 절개술 5예, 전자궁 적출술 2예, 난소 종양 2예에 투여하였다. EPO를 투여 받은 9예에서는 평균 혈색소 수치가 수술 전에 비해 수술 후 오히려 의미있게 증가하였다. 조직학적 결과로는 자궁 선근증 9예, 평활근종 8예, 생식 세포종 3예, 자궁외 임신 2예, 자궁 상피내 종양 1예, 상피성 난소 종양 1예, 출혈성 황체 낭종 1예, 성기삭 간질성 종양 1예로 총 27예이었다. 무혈 수술로 인한 술 후 사망은 없었으며 이환율도 적었다. 결론 : 여호와의 증인 환자에 대한 산부인과적 수술이 요구될 때 세심한 수술 기법과 다양한 혈액 보존법 등을 이용한다면 비교적 양호한 무혈 수술의 결과를 얻을 수 있었다. 그러나 향후 여호와의 증인 환자에 대한 특수화된 프로그램의 개발이 필요하다고 사료되었다. Objective : Jehovah's Witnesses are members of a religious denomination whose beliefs prohibit the use of blood or blood products. Plasma volume expanders and extracorporeal hemodilution of the patients's own blood are theologically acceptiable. And so we evaluated effectiveness of major operative procedures carried out on Jehovas's Witnesses patients without blood transfusions or blood products. Methods : We retrospectively studied 50 cases of Jehovas's Witnesses who underwent obstetric and gynecologic bloodless surgery from February 1995 to August 1999. No patients recieved any blood or blood products during hospitalizaion. Results : The age distribution of patients was between 9 years and 58 year, average 36.7±8.7 years old. The most common cases for bloodless surgery was Cesarean section. The recombinant human erythropoietin(EPO) was treated in 5 cases of Cesarean section, 2 cases of TAH and 2 cases of ovarian tumor perioperatively. EPO-treated 9 cases were increased postoperative mean Hb levels compared with preoperative mean Hb levels. Histology revealed 9 cases of adenomyosis, 8 cases of leiomyoma, 3 cases of germ cell tumor, 2 cases of ectopic pregnancy, 1 case of CIS, 1 case of Krukenberg tumor, 1 case of epithelial cell tumor, 1 case of hemorrhagic corpus tumor and 1 case of sexcord stromal tumor, total 27 cases. There was no operative death and postoperative morbidities were few. Conclusions : We concluded that if we preformed the surgeries with careful operative techniques and various blood conserving procedures we could do the bloodless surgery of Jehovah's Witnesses with relative safety. But the establishment of a well programmed special medical supports for the Jehovah's Witnesses community will be needed in future.