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The role of soluble common gamma chain in autoimmune disease
Byunghyuk Lee,홍창완 대한해부학회 2015 Anatomy & Cell Biology Vol.48 No.1
The common gamma chain (γc) is the central signaling unit for a number of cytokine receptors collectively known as the γc cytokine receptor family. γc is critical for ligand binding and signaling by γc cytokines. γc cytokine signaling had been thought to be mainly regulated by cytokine-specific receptor α chain expression levels with little or no effect by γc surface levels because γc expression was presumed to remain unchanged during T-cell activation and development. The extent of γc cytokine responses is thought to be regulated by cytokine specific receptor subunits and not by the γc receptor. In contrast to this prevailing view, we have recently reported that γc itself actively regulates γc cytokine responses. Interestingly, γc exerted its regulatory effects not only as a conventional membrane receptor protein but also as a secreted protein whose expression was upregulated upon T-cell stimulation. Here we will review how a soluble form of γc, which is generated by alternative splicing, regulates γc cytokine signaling and plays a role in controlling immune activation related to autoimmune disease.
Dazl can bind to dynein motor complex and may play a role in transport of specific mRNAs
Lee, Kyung Ho,Lee, Seongju,Kim, Byunghyuk,Chang, Sunghoe,Kim, Soo Woong,Paick, Jae-Seung,Rhee, Kunsoo Wiley (John WileySons) 2006 The EMBO journal Vol.25 No.18
<P>Male germ cell development includes mitotic and meiotic cell divisions that are followed by dramatic morphological changes resulting in the production of spermatozoa. Genetic evidence has indicated that the DAZ family genes are critical for successful male germ cell development in diverse animals as well as humans. In the present study, we investigated the cellular functions of Dazl in the mouse male germ cells. We identified a specific interaction of Dazl with the dynein light chain, a component of the dynein-dynactin motor complex. The subcellular distribution of Dazl was microtubule-dependent and a selected number of Dazl-bound mRNAs could accumulate in the perinuclear area. Based on these results, we propose that Dazl may play a role in transport of specific mRNAs via dynein motor complex. The Dazl-bound mRNAs may be stored at specific sites and would be available for future developmental processes. Our study revealed the presence of an active mRNA transport system in mouse male germ cells.</P>
Polymorphic expression of DAZ proteins in the human testis
Kim, Byunghyuk,Lee, Youngbin,Kim, Yeonwha,Lee, Kyung Ho,Chun, Sunhye,Rhee, Kunsoo,Seo, Ju Tae,Kim, Soo Woong,Paick, Jae-Seung Oxford University Press 2009 Human reproduction Vol.24 No.6
<P>BACKGROUND:DAZ is a male infertility gene located at the AZFc region of the Y chromosome. There are four copies of the DAZ gene that share a strong homology but are not identical to one another. In the present study, we carried out cDNA cloning and immunoblot analyses to determine whether all of the DAZ genes are actively expressed in the human testis. METHODS:AZFc deletion was detected by sequence-tagged site polymerase chain reaction (PCR) of genomic DNA isolated from blood samples. DAZ cDNAs were cloned with RT-PCR followed by sequence analysis. The expression of DAZ proteins in human testis was determined by immunoblot and compared with DAZ cDNA expression. RESULTS:Immunoblot analysis revealed four DAZ protein bands in testis samples that showed no deletions in the AZFc region. No specific bands were observed in samples from AZFc deletion patients. Testis samples from individuals with the partial AZFc deletion, gr/gr, showed two DAZ-specific bands. Interestingly, the sizes of DAZ-specific bands varied among individuals. Analysis of DAZ transcripts in testis samples revealed that the DAZ proteins were translated from the largest of the multiple transcripts originating from each single DAZ gene. CONCLUSIONS:All four DAZ genes are expressed in the human testis, and their products are highly polymorphic among men.</P>
Analysis of DAZ gene expression in a partial AZFc deletion of the human Y chromosome
Kim, Byunghyuk,Lee, Wonkyung,Rhee, Kunsoo,Kim, Soo Woong,Paick, Jae-Seung CSIRO Publishing 2014 Reproduction, fertility, and development Vol.26 No.2
<P> The azoospermia factor c (AZFc) region of the Y chromosome consists of repetitive amplicons and is therefore highly susceptible to structural rearrangements, such as deletions and duplications. The b2/b3 deletion is a partial AZFc deletion that is conventionally determined by the selective absence of sY1191 in sequence-tagged site polymerase chain reaction (PCR) and is generally believed to retain two of the four deleted in azoospermia (DAZ) genes on the Y chromosome. In the present study we determined the copy number and expression of DAZ genes in sY1191-negative individuals. Using a DAZ dosage PCR assay and Southern blot analysis we evaluated the expression of four DAZ genes in five of six sY1191-negative individuals. Furthermore, cloning and immunoblot analyses revealed that three or more DAZ genes are expressed in sY1191-negative testes with germ cells. The results indicate that the selective absence of sY1191 not only means b2/b3 deletion with two DAZ genes, but also includes another AZFc configuration with four DAZ genes. These results exemplify the prevalence of variations in the AZFc region of the human Y chromosome. </P>
Yu, Byunghyuk,Park, Ki Bum,Park, Ji Yeon,Lee, Seung Soo,Kwon, Oh Kyoung,Chung, Ho Young The Korean Gastric Cancer Association 2019 Journal of gastric cancer Vol.19 No.3
Purpose: Phase angle obtained using bioelectrical impedance analysis (BIA) provides a relatively precise assessment of the nutritional status of elderly patients. This study aimed to evaluate the significance of phase angle as a risk factor for complications after gastrectomy in elderly patients. Materials and Methods: We evaluated 210 elderly patients (aged ${\geq}65years$) who had undergone gastrectomy for gastric cancer between August 2016 and August 2017. The phase angle cutoff value was calculated using receiver operating characteristic curve analysis according to sex. A retrospective analysis regarding the correlation between early postoperative complications and well-known risk factors, including the phase angle, was performed. Results: Multivariate analysis revealed that the presence of two or more comorbidities (odds ratio [OR], 3.675) and hypoalbuminemia (OR, 4.059) were independent risk factors for overall complications, and female sex (OR, 2.993) was independent risk factor for severe complications. A low phase angle (OR, 2.901 and 4.348, respectively) and total gastrectomy (OR, 4.718 and 3.473, respectively) were independent risk factors for both overall and severe complications. Conclusions: Our findings show that preoperative low phase angle predicts the risk of overall and severe complications. Our findings suggest that BIA should be performed to assess the risk of postoperative complications in elderly patients with gastric cancer.
Collateral effect of the COVID-19 pandemic on emergency department visits in Korea
여인환,김종근,( Byunghyuk Yu ),( Kang-suk Seo ),( Jung-bae Park ),( Yeonjoo Cho ),( Jae Young Choe ),( Changho Kim ),( Yun Jeong Kim ),( Dong Eun Lee ),( In Hwan Yeo ),( Won-kee Lee ) 대한응급의학회 2022 대한응급의학회 학술대회초록집 Vol.2022 No.2
하이브리드차량 NVH 성능확보를 위한 서브프레임 및 엔진마운트 최적화
박상영(Sangyoung Park),이병혁(Byunghyuk Lee),방정환(Junghwan Bang),박동철(Dongchul Park) 한국자동차공학회 2010 한국자동차공학회 부문종합 학술대회 Vol.2010 No.5
In this study, the NVH performances of hybrid car pursuing competitive fuel economy were secured by optimizing the front platform. The booming which was increased by removing BSM was improved by changing the subframe shape and engine mounting type. And, the shock which was inherent from power flow mechanism of hybrid car was improved by optimizing the stiffness and stopper gap of the engine mounts.
Kim, Geona,Jo, Yuna,Lee, Byunghyuk,Ali, Laraib Amir,Lee, Boae,Hong, Changwan MDPI 2018 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.19 No.11
<P>IL-7 is an essential, nonredundant growth factor for T and B cell generation and maintenance. While IL-7 deficiency results in lymphopenia, overexpression of IL-7 can cause neoplasia in experimental models. IL-7’s involvement in neoplasia has been appreciated through studies of IL-7 transgenic (Tg) mice models and human lymphoma patients. Since we recently found that a soluble form of the common γ-chain (γc) cytokine receptor (sγc) antagonistically regulates IL-7 signaling, IL-7 and sγc double-Tg mice were generated to investigate the effects of sγc overexpression in IL-7-mediated lymphoproliferative disorders (LPDs). The overexpression of sγc prevents IL-7Tg-induced abnormal increase of LN cell numbers and the development of splenomegaly, resulting in striking amelioration of mortality and disease development. These results suggest that modification of γc cytokine responsiveness by sγc molecules might control various γc cytokine-associated hematologic malignancy, and also provide an alternative view to approach antitumor therapy.</P>