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CITES 분류에 의한 사향(麝香)의 기원 동물 분류 및 특성에 대한 고찰
장문석,김도림,김현철,박성규,Chang, Mun Seog,Kim, Do Rim,Kim, Hyun Chul,Park, Seong Kyu 대한한의학방제학회 2016 大韓韓醫學方劑學會誌 Vol.24 No.4
Objectives : The purpose of this study was to investigate musk deer in taxonomic status and genus species. We investigated the characteristic of musk deer for medicinal usages. Methods : To identifications of musk deer on the taxonomic status and genus species, the literary investigation were conducted on the Korean, China, and Japan pharmacopoeia and published herbal books, CITES Species Lists too. Results : Musk deer placement in a separate family status is the Moschidae. M. chrysogaster Hodgson and M. sifanicus Przewalski was the same species. So, Alpine musk deer revised M. chrysogaster Hodgson[=M. sifanicus Przewalski]. Geographic distribution of M. moschiferus L. divided Sibirica group and himalaica group. Group himalaica contains three subspecies: Korean musk deer(M. moschiferus parvipes Hol.), Chinese musk deer(M. chrysogaster Hodgson), and Himalayan musk deer(M. leucogaster Hodgson). The genetic divergence between M. moschiferus L. and other species was clearly distinguished from the others. M. berezovskii Flerove was less than the others. However, the divergence among M. chrysogaster Hodgson, M. fuscus Li, and M. leucogaster Hodgson were quite low. Musk deers are mostly distributed around the high-plateau. Moschus were from Nepal, Bhutan, Russia, China. Forest musk deer(M. berezovskii Flerove) farming was conducted in China from 1950s. In the Korean hebal pharmacopoeia, Moschus include l-muscone($C_{16}H_{30}O$ : 238.40) over 2% for quantitative test. Conclusions : There are three species of musk deer, Siberian musk deer(M. moschiferus L.), forest musk deer(M. berezovskii Flerove), and Alpine musk deer(M. chrysogaster Hodgson) for medicinal usages.
장문석,오명숙,이병희,양웅모,김원남,김도림,김향미,박은화,박완수,김윤경,박성규,Chang, Mun-Seog,Oh, Myung-Sook,Lee, Byong-Hee,Yang, Woong-Mo,Kim, Won-Nam,Kim, Do-Rim,Kim, Hyang-Mi,Park, Eun-Hwa,Park, Wan-Su,Kim, Yun-Kyung,Park, Seong-Ky 대한한의학방제학회 2006 大韓韓醫學方劑學會誌 Vol.14 No.1
The purpose of this study was to investigate the effect of water extract of Dioscoreae Rhizome on the reproduction activity of Wistar male rat. The group treated with 1.0 g/kg/day oral administrations of water extract of Dioscoreae Rhizoma (SK0l6) during 8 weeks was compared with the normal group. Sperm count, sperm motility, body weight, and testis weight were investigated in two groups. Sperm count. body weight, and testis weight of two groups did not show the significant difference. But sperm motility of the treated group was reduced significantly (the normal group: 56.43 %, SK0l6 group: 53.47 %, p < 0.05). According to the results, SK0l6 have no effects on sperm count, body weight. and testis weight but reduced sperm motility significantly.
쇄양(鎖陽)의 Diphenyl-picryl-hydrazyl (DPPH) 소거 활성 및 HepG2 세포에 대한 항산화 효과
장문석,양웅모,김도림,박은화,박수연,박성규,Chang, Mun-Seog,Yang, Woong-Mo,Kim, Do-Rim,Park, Eun-Hwa,Park, Soo-Yeon,Park, Seong-Kyu 대한한의학방제학회 2007 大韓韓醫學方劑學會誌 Vol.15 No.2
The purpose of this study was to investigate the anti-oxidant effect of Cynomorium songaricum. The extract of Cynomorii Herba was studied for diphenyl-picryl-hydrazyl (DPPH) radical scavenging activity, HepG2 cell viability and $H_2O_2$-induced cytotoxicity by a modified MTT assay. DPPH radical scavenging activity was measured after 30 minutes. The extract was tested by 1. 5, 10, 50, 100 and 500 ${\mu}g/ml$ concentrations. HepG2 cell viability by a modified MTT assay was measured in the concentrations of 10, 50, 100, 250, 500 ug/ml for 24 h. The results showed that the extract scavenged DPPH radical up to 52.2% with 50 ug/ml concentration. The extract did not reduced the cell viability and $H_2O_2-induced$ cytotoxicity (69.4%) was blocked by the extract in the concentrations of 50, 100, 250 and 500 ${\mu}g/ml$. In conclusion, the extract of Cynomorii Herba has potent antioxidant activity.
남성생식세포 Sertoli cell에 미치는 복분자(覆盆子)의 항산화 효과
김영주,장문석,박성규,Kim, Young Joo,Chang, Mun Seog,Park, Seong Kyu 대한한의학방제학회 2018 大韓韓醫學方劑學會誌 Vol.26 No.2
Objectives : The purpose of this study was to examine the antioxidant effects of the extract of Rubi Fructus on TM4 Sertoli cells. Methods : The extract was studied for diphenyl-picryl-hydrazyl (DPPH) radical scavenging activity and cell viability assays on Sertoli cells. In addition, hydrogen peroxide-induced oxidative stress on Sertoli cells were examined by MTT assay. The antioxidant enzyme of Cu/Zn SOD, Mn SOD, catalase protein expression on Sertoli cells were also measured. Results : The results showed that the extract scavenged DPPH radical dose-dependent manner. The extract showed no cytotoxicity at concentration of 1, 5, 10, 50, $100{\mu}g/ml$. The hydrogen peroxide-induced cytotoxicity of Sertoli cells was protected to 88.3% by the extract at concentration of $100{\mu}g/ml$. Cu/Zn SOD and Mn SOD protein expression were significantly increased on Sertoli cells, but catalase protein expression was not significantly changed. Conclusions : In conclusion, the extract of Rubi Fructus has antioxidant effects on Sertoli cells and protect male reproductive system against oxidative stress.
Leydig Cell의 항산화에 미치는 벌사상자와 사상자의 비교연구
오지훈 ( Ji Hoon Oh ),김도림 ( Do Rim Kim ),박수연 ( Soo Yeon Park ),장문석 ( Mun Seog Chang ),박성규 ( Seong Kyu Park ) 대한본초학회 2014 大韓本草學會誌 Vol.29 No.6
Objectives : The purpose of this study was to estimate the antioxidant activity of water extract of Cnidii Fructus (CF) and Torilis Fructus (TF) in Leydig cells. Methods : Free radical scavenging activity of CF and TF against 2,2-diphenyl-1-picrylhydrazyl (DPPH) was determined spectrophotometrically. We investigated the effect of CF and TF in Leydig cells by MTT assay. The protective effects of CF and TF against hydrogen peroxide-induced oxidative stress in Leydig cells. Superoxide dismutase (SOD), and catalase activity assays were performed in Leydig cells. Results : The results showed that CF scavenged DPPH radical in a dose-dependent manner by up to 81.2%, TF scavenged DPPH radical in a dose-dependent manner by up to 63.8%. CF showed cell viability as 121.0, 132.7, 126.6% in 5, 10, 100 μg/ml concentrations. TF showed cell viability as 127.5, 111.8% in 5, 100 μg/ml concentraions, respectively. The hydrogen peroxide-induced cytotoxicity of Leydig cells were protected to 86.3% by CF at concentration of 10 μg/ml and protected to 83.5% by TF at concentration of 100 μg/ml. Both CF and TF at all concentrations, SOD activity was not significantly changed. Catalase activity was significantly increased at 10, 100 μg/ml concentrations of CF, respectively. TF`s catalase activity showed no significant difference from that of the control. Conclusions : These results suggest that CF, as an antioxidant, protects Leydig cells in hydrogen peroxide-induced oxidative stress. know that『Kwangjebikeup』played a role in settlement and spreading of foreign knowledge to civilians.
흑지마(黑芝麻)가 Leydig cell의 항산화에 미치는 영향
장문석 ( Mun Seog Chang ),정규진 ( Kyu Jin Chung ),장원규 ( Won Kyu Chang ),박성규 ( Seong Kyu Park ) 대한본초학회 2011 大韓本草學會誌 Vol.26 No.1
Objectives: The purpose of this study was to estimate the antioxidant activity of Sesami Semen Nigrum extract (SSN) on mouse Leydig cells, TM3. Methods: Cell viability assays were performed. The protective effects of SSN against hydrogen peroxide-induced oxidative stress in Leydig cells were examined by measuring cell viability. Lipid peroxidation levels and antioxidant enzyme concentrations such as SOD and catalase were measured. Results: Cell viability of Leydig cells increased with SSN concentration. Cell viability of Leydig cells was 136.66% when SSN concentration was 50 μg/ml. Cell viability of the hydrogen peroxide group was statistically decreased (p<0.01) compared with the control group. Antioxidant effect of SSN was measured and the protective effect of SSN concentration were 5, 10, 50 μg/ml. LPO were decreased significantly at 5, 50 μg/ml of SSN concentrations. SOD activity was increased at 1, 10, 50 ug/ml of SSN concentrations. Catalase activity was significantly increased at 123.7, 133.3 and 131.9 units/mg protein when SSN concentrations were 5, 10 and 50 μg/ml, respectively. Conclusions: In conclusion, Sesami Semen Nigrum extract has antioxidant activities in Leydig cells against oxidative stress.
연잎, 연꽃, 연꽃 수술 추출물이 UVB 자외선 조사에 의한 각질형성세포의 보호 및 피부 노화 방지에 미치는 영향
장문석 ( Mun Seog Chang ),고은빛 ( Eun Bit Ko ),이호진 ( Ho Jin Lee ),김주성 ( Ju Sung Kim ),김진수 ( Jin Soo Kim ),지성원 ( Sung Won Jee ),김휴영 ( Hyu Young Kim ),염명훈 ( Myeong Hoon Yeom ),김덕희 ( Duck Hee Kim ),김한곤 ( Han 대한본초학회 2011 大韓本草學會誌 Vol.26 No.2
Objectives: The purpose of this study was to investigate the anti-aging effects on cultured human keratinocytes with Nelumbo nucifera extracts. Methods: Each parts of leaves, flowers and stamen were extracted with water or 70% ethanol. These extracts were tested for cell viability on HaCaT cells (human keratinocyte line) by MTT assay. We investigated the effects of Ultraviolet-B (UVB) irradiation on cytotoxicity and lipid peroxidation in cultured skin keratinocytes. Results: The ethanol extract of Nelumbo nucifera flowers showed maximun cell viability as 111.39% in 30 ug/ml concentration. The water extracts of stamen, flowers, leaves showed cell viability as 107.12, 101.65, 101.46%, respectively. HaCaT keratinocytes were survived 63.06% at 20 mJ/cm2 UVB irradiation. The cell membrane lipid peroxidation was measured by accumulation malondialdehyde (MDA). The levels of MDA were decreased by the ethanol extract of Nelumbo nucifera flowers and the water extracts of stamen. Conclusions: These finding suggest that the ethanol extract of Nelumbo nucifera flowers prevent anti-aging effects on cultured human keratinocytes during UVB irradiation.