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Argyrophilic Fibers of Intravascular Threadlike Structures in Rat
이병천,남태정,정현민,박은성,백구연,성백경,소경순,윤여성,소광섭,Lee, Byung-Cheon,Nam, Tae-Jeong,Johng, Hyeon-Min,Park, Eun-Sung,Baik, Ku-Youn,Sung, Baeck-Kyoung,Soh, Kyung-Soon,Yoon, Yeo-Sung,Soh, Kwang-Sup KOREAN PHARMACOPUNCTURE INSTITUTE 2008 Journal of pharmacopuncture Vol.11 No.3
Objective: We took intravascular threadlike structures from rat aortas to investigate their histological characteristics consistent with the intravascular Bonghan duct. Methods: Gomori s silver impregnation method, in addition to routine hematoxylin and eosin staining, was applied to demonstrate the characteristic feature of the intravascular threadlike structures. Results: These two staining methods clearly showed that the intravascular threadlike structures had unique features of argyrophilic reticular fibers and heavily stained oval or rod-shaped nuclei in them. Conclusion: The results are strong evidences for identifying threadlike structure as the intravascular Bonghan duct.
마우스 장기표면 봉한관 및 봉한소체의 발견 방법에 관한 연구
이병천,이창훈,소경순,강대인,소광섭,Lee, Byung-Cheon,Lee, Chang-Hoon,Soh, Kyung-Sun,Kang, Dae-In,Soh, Kwang-Sup 대한약침학회 2006 Journal of pharmacopuncture Vol.9 No.3
Though anatomical and histological investigations on the novel threadlike and corpuscular structures, Bonghan duct(BHD) and Bonghan corpuscles(BHC) of internal organs of rats and rabbits were extensively studied. In this article we introduce the refined method to observe the BHD and BHC on the internal organs of mice. This development is needed because immunohistochemical analysis and other modern biological techniques were mostly applicable only to mice but not to rats and rabbits. We made uses of grazing effect of light as well as discriminating technique of BHD/C from fibrin and take the novel structures, BHD/C on mouse internal organ. The specimens taken were examined by hematoxyline and eosin(H & E) and revealed as novel structures. Our method described herein to take BHD/C characterization of mouse, especially for stem cell related researches.
DiI Staining of Fine Branches of Bonghan Ducts on Surface of Rat Abdominal Organs
이병천,Seong-Uk Jhang,Jae-Hong Choi,So-Yeong Lee,Pan-Dong Ryu,소광섭 사단법인약침학회 2009 Journal of Acupuncture & Meridian Studies Vol.2 No.4
Novel thread-like structures and corpuscles, designated Bonghan ducts (BHDs) and corpuscles (BHCs), are known to form a system of networked channels. Here, we tested the effectiveness of a fluorescent carbocyanine dye, DiI (1,1'- dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate), in staining BHDs and BHCs. DiI solution was infused into a BHC on the surface of a rat abdominal organ at a steady rate and the resulting labeling of neighboring BHCs connected via BHDs was examined, as identified by the red fluorescence of DiI. BHDs diameters tapered away from BHCs and formed tree-like branches with fine arborizations embedded in the membranous tissues at their terminal parts. In the proximal parts, DiI fluorescence appeared as continuous lines within BHDs, but a large portion of BHDs remained unstained. In the distal parts of BHDs, discontinuous elongated DiI microparticles were identified along the sinuses within BHDs. The results showed that inner spaces within the BHDs allowed DiI to flow and that BHDs have tree-like branches and terminal arborizations. In conclusion, DiI can be used in visualizing BHDs fine structures.
이병천,김정순,허용,Lee Byeong-Cheon,Kim Jung-Sun,Heo Yong The Korean Society of Veterinary Clinics 1991 한국임상수의학회지 Vol.8 No.2
The study on leptospiral infection among cows was carried out to find out possible role of this domestic animal as an infection source for humans, and the magnitude of economic loss due to abortions caused by leptospiral infections among the domestic animal. Bood samples of 459 cows in May and 446 heads in November 1989 were collected from 19 villages of Kyunggi Province. These samples were cultured and serologically tested. Only one strain of leptospires, most strongly reactive to Pomona antibody, was isolated from duplicated cultures of 985 cow blood samples in EMJH medium. Serological test by MAT with seven reference strains and three locally isolated strains was positive in 12% of the sample in May and 26% in November ; positive rate increased slightly as the age of cows increased and male cows had higher positive rate, however, there was on difference in positive rate by type of cow and area. There was no statistically significant association between abortion history among cows(one yearperiod) and positive MAT. Thus it was concluded that the leptospiral infection of cows may play a role for human infection, and the leptospiral infection of cow may not be the major cause of abortion although further study is necessary for definite conclusion. 경기도의 19부락에서 1989년 5월에 459두와 그해 11월에 446두의 소를 대상으로 하여 렙토스피라 감염유무를 조사하였다. 혈액배양에서는 포모나 항체에 강하게 반응하는 한 균주만이 검출되었다. 몇개의 표준균주를 사용한 혈청검사에서는 5월에 12%, 11월에 26%의 양성율을 나타내었다. 년령이 증가함에 따라 양성율이 높아졌으며 숫소가 암소보다 높았다. 혈청검사 양성과 소의 유산력과는 유의적인 관계가 인정되지 않았다.
내연산(內延山) 일대(-帶) 삼림식생(森林植生)의 군락분류(群落分類)에 관(關)한 연구(硏究)
이병천,윤충원,Lee, Byung-Chun,Yun, Chung-Weon 한국생태학회 2002 Journal of Ecology and Environment Vol.25 No.3
내연산의 삼림식생을 대상으로 ZM식물사회학적 방법에 의해 군락단위 및 그 하위단위를 분류하고, 분류된 각 단위와 환경사이의 상관관계를 서열법으로 해석하여 내연산 산림식생관리를 위한 기초자료를 제공할 목적으로 본 연구를 수행하였다. 군락단위는 신갈나무군락, 굴참나무군락, 소나무군락, 서어나무군락, 느티나무군락이 구분되었고, 하위단위는 신갈나무군락에서 철쪽꽃군과 참회나무군, 굴참나무군락에서 우산나물군, 털조록싸리군 및 찰피나무군, 느티나무군락에서 까치박달군과 고욤나무군이 각각 세분되었다. 따라서 내연산 일대의 산림은 5개군락, 7개군으로 총 9개의 식생단위로 분류되었다. CCA 방법으로 식생과 환경사이의 상관관계 분석 결과 내연산 지역의 식생단위는 해발과 가장 높은 상관관계가 있는 것으로 나타났다. 신갈나무군락의 철쪽꽃군과 참회나무군은 해발고와 지형이 높은 곳과 노암율이 낮은 곳에 각각 분포하는 경향이었다. 한편 느티나무군락은 신갈나무군락과 역의 관계로 나타났다. 앞으로 산림식생관리시 특히 계곡림에 대해서는 교목층의 우점종들이 상관적으로 이질적인 모습을 하고 있더라도 하나의 관리단위로 고려되어야 할 것으로 판단되었다. This study was carried out to classify forest vegetation of the Mt. Naeyeon with phytosociological analysis of ZM school and to explain ordination of communities with CCA. The research sites were located between the northern part of Pohang-si area and the southern part of Yeongdeok-gun area. The 70 plots consisted of 253 species were investigated. The forest vegetation was classified into Quercus mongolica community, Q. variabilis community, Pinus densiflora community, Carpinus laxiflora community and Zelkova serrata community. Q. mongolica community was divided into 2 groups such as Rhododendron schlippenbachii group and Euonymus oxyphyllus group and Q. variabilis community was divided into 3 groups such as Syneilesis palmata group, Lespedeza$\times$tomentilia group and Tilia mandshurica group, and Z. serrata community was divided into 2 groups such as Carpinus cordata group and Diospyros lotus group. According to the results of CCA ordination, Q. mongolica community showed high positive correlation to altitude and topography, whereas it showed negative correlation to bare rock. But Z. serrata community showed the opposite tendency to Q. mongolica community, Altitude was considered as the most important factor among 5 environmental variables in the correlation with axes.
Fluorescent Method for Observing Intravascular Bonghan Duct
이병천,백구연,정현민,성백경,소경순,강대인,소광섭,Lee, Byung-Cheon,Baik, Ku-Youn,Johng, Hyeon-Min,Sung, Baeck-Kyoung,Soh, Kyung-Soon,Kang, Dae-In,Soh, Kwang-Sup KOREAN PHARMACOPUNCTURE INSTITUTE 2005 Journal of pharmacopuncture Vol.8 No.3
미분간섭현미경법과 아크리딘-오렌지(acridine-orange) 형광 염색을 이용하여 쥐 혈관 내의 실 모양 구조물을 관찰하였다. 공초점현미경법과 헤마톡실린-에오신(hematoxylin-eosin) 염색을 통해 피브린, 모세혈관, 소정맥, 소동맥 혹은 림프관의 핵 분포와 뚜렷이 구별되는 혈관 내 실 모양 구조물의 핵 분포 패턴을 얻어낼 수 있었다. 이 실 모양 구조물의 생리적 기능을 침술과 연관하여 논의하였다. 특히, 이 실 모양 관을 통한 약물의 흐름이 약침의 기전에 해당할 것이라는 가설을 제기하였다. Observation of intra-vascular threadlike structures in the blood vessels of rats is reported with the images by differential interference contrast microscope, and fluorescence inverted microscope of the acridine-orange stained samples. The confocal microscope image and the hematoxylin-eosin staining revealed the distinctive pattern of nuclei distribution that clearly discerned the threadlike structure from fibrin, capillary, small venule, arteriole, or lymph vessel. Physiological function of the intra-vascular thread in connection with acupuncture is discussed. Especially, this threadlike duct can be a circulation path for herb-liquid flow, which may provide the scientific mechanism for therapeutic effect of herbal acupuncture.
Trypan blue 도포를 사용한 누드 마우스와 흰쥐에서 경혈자리 찾기와 DiI 추적 법
이병천,엄기훈,배경희,강대인,소광섭,Lee, Byung-Cheon,Uhm, Ki-Hoon,Bae, Kyoung-Hee,Kang, Dae-In,Soh, Kwang-Sup 대한약침학회 2009 Journal of pharmacopuncture Vol.12 No.3
Objectives: To find the potential acupuncture points by using Trypan blue staining on the skin of rat and Nude mouse. Methods: 0.4% Trypan blue was applied to the skin of rat or Nude mouse previously treated by surfactant. Washing by warm saline was followed after enough application of trypan blue and surfactant. Frequency of Trypan blue application should be varied to the experimental animals' condition for visualizing significant spots. Results: Blue spots appeared roughly in symmetry along kidney meridian or stomach meridian. Several spots outside of kidney or stomach meridian were also observed; however, the detail stereoscopic images of those blue spots were slightly different according to the position blue-colored. DiI signals were visualized along blood vessel after DiI injection into the Trypan blue-visualized blue spots. Conclusion: Our method to visualize the potential acupuncture points as a blue spot on rat and Nude mouse skins may contribute to the next step for finding specific flowing channels among blue spots.