자동 분주기 Fixed Tip의 Carryover 방지를 위한 개선 방안

황보라,어두희,배진수,박종오,김지영,석재동,Hwang, Bo-Ra,Au, Doo-Hee,Bae, Jin-Su,Park, Jong-O,Kim, Ji-Young,Seok, Jae-Dong 대한핵의학기술학회 2009 핵의학 기술 Vol.13 No.3

목적 : 자동 분주기 (TECAN)의 fixed tip 사용 시 충분한 세척에도 불구하고 고농도 검체 뒤에 연이은 검체가 영향을 받는 carryover 현상이 발생되는 경우가 있다. 이는 검사결과가 위 양성으로 나타날 수 있는 위험성이 있으므로 이상유무 판단을 위해 잦은 재검을 실시해야 하는 번거로움이 있다. 따라서 본 연구는 경제성이 높은 fixed tip을 사용하면서 carryover를 방지할 수 있는 방법을 개선하고자 한다. 실험재료 및 방법 : 자동분주기(Tecan)를 이용하여 HBs Ag, HBs Ab, HBc Ab(IgG) 검사를 대상으로 실행하였으며, fixed tip 방식의 분주시스템으로 일반 환자의 검체 분주 후에 생리식염수를 분주 할 수 있도록 배치하였다. 세척 단계는 0.25mol/L NaOH용액을 이용하였으며, 생리식염수의 검사결과 cpm을 측정하여 carryover의 발생 유무를 확인하였다. 또한 3가지 검사를 0.25 mol/L NaOH 용액이 검사에 미치는 영향을 분석하였다. 결과 : 생리식염수 결과는 100% 음성결과를 보였으며, 0.25 mol/L NaOH를 세척액으로 사용한 fixed tip에서 검체분주 시 환자 혈청은 HBc Ab (IgG)검사에서 cut-off zone (cut-off${\pm}$10%)을 제외한 모든 결과에서 기존 외래보고 결과와 동일한 값을 얻었다. 결론 : 생리식염수 검사결과 100% 음성을 보인 것은 실험에서 사용한 세척액으로 fixed tip을 세척하는 방법이 carryover를 방지할 수 있고, 이로 인해 발생하는 위양성을 방지할 수 있었다. 또한, 환자 검체의 결과 값이 기존 보고 결과와 일치함으로 0.25 mol/L NaOH 용액이 간염 검사결과에 영향을 끼치지 않음을 확인하였다. 따라서 본 실험에서 실시한 방법이 disposable tip보다 상대적으로 경제적인 fixed tip을 사용하면서도 carryover가 없는 정확한 결과를 얻을 수 있는 효과적인 방안임을 알 수 있다. Purpose: Automated system that immunoassay examination are used widely. However, sample to sample carryover can cause that the next patient sample is false positive. Materials and Methods: We test HBs Ag, HBs Ab, HBc Ab(IgG) with Automated pipetting system (Tecan). It was placed with very high concentrations followed by saline solution. During this experiment, The fixed tip of Automated system wash With 0.25 moL/L NaOH. The Measurement results of saline solution confirm the occurrence of carryover. Results: Results of saline solution with 0.25 moL/L NaOH cleaning process was measured 100% negative, And results of patient serum with 0.25 moL/L NaOH cleaning process is similar reported results. Conclusion: As Results, 0.25 moL/L NaOH cleaning process was avoid carryover in experiment, And we know results of the hepatitis test did not affected by this solution we recommend 0.25 moL/L NaOH cleaning process as the Prevention of carryover in the automated system with fixed Tips.

Cellular system에서의 깻잎의 ONOO^-에 의한 산화적 스트레스 개선 및 항노화 효과

김현영,황보라,조은주,Kim, Hyun Young,Hwang, Bo Ra,Wu, Ting Ting,Cho, Eun Ju 충남대학교 농업과학연구소 2012 농업과학연구 Vol.39 No.4

In this study, we investigated the antioxidative and antiaging activity of Perilla frutescens using LLC-$PK_1$ porcine renal epithelial cell and WI-38 human diploid fibroblast cell. The extract from Perilla frutescens showed strong protective effect against nitric oxide (NO) and superoxide ($O_2{^-}$)-induced oxidative stress generated by sodium nitroprusside (SNP) and pyrogallol, respectively. The result showed that P. frutescens increased the cell viability and showed scavenging activity of NO and $O_2{^-}$. In addition, the extract of P. frutescens exerted the protective effect against peroxynitrite ($ONOO^-$) induced by 3-morpholinosydnonimine. It suggests that P. frutescens would have the protective role against $ONOO^-$ itself and its precursors, NO and $O_2{^-}$. Furthermore, the aging model of hydrogen peroxide ($H_2O_2$)-treated WI-38 human diploid fibroblast was employed to investigate the anti-aging effect of P. frutescens. $H_2O_2$-treated WI-38 cells showed the loss of cell viability, however before-treatment with P. frutescens to WI-38 cells under premature senescence could delay the cellular aging process. The present study suggests the antioxidative and antiaging potential against free radical-induced oxidative damage of P. frutescens.

타이어 파열 사망 사례에 대한 고찰

김대열(Kim, Dae Yoil),황보라(Hwang, Bo Ra),이진식(Lee, Jin Sik),이상한(Lee, Sang Han) 경찰대학 경찰학연구편집위원회 2021 경찰학연구 Vol.21 No.2

타이어 파열에 의한 사망 사고는 극히 드물게 언론을 통해 알려진 사실이 있지만, 아직 국내 사례가 문헌에 보고된 적은 없다. 25톤 덤프트럭 운전자인 50대 남성이 배송 작업을 마치고 차고지에 돌아와 주차 후 차량을 살피던 중 때마침 변사자 전면에 위치한 타이어가 파열되면서 높은 공기압으로 인해 공중으로 날려가 바닥에 떨어지는 사고로 사망했다. 변사자는 양쪽 귀 출혈, 턱과 복부에 파열된 타이어 강철선에 의한 것으로 추정되는 정형손상, 다수의 표피 박탈, 상체에 심한 피하 기종 등의 손상이 관찰되었다. 덤프트럭 타이어 파열은 일반적인 차량 타이어 펑크와 달리 폭발물 손상에 준하여 현장조사 및 검시를 해야 할 필요성이 있다. 타이어 안전사고와 관련된 정책적 제도를 검토하고 향후 사고 예방을 위한 방안을 제안 한다. Fatal accidents caused by tire burst have been known through the media very rarely, but no domestic cases have been reported in the literature yet. a man in his 50s who drove a 25-ton dump truck returned to the garage after completing the delivery work and was looking at the vehicle after parking, when a second tire ruptured located in front of the passenger seat, causing high air pressure to fly to the ground. The victim was observed with bleeding both ears, orthopedic damage presumed to be caused by tires and wires ruptured in the jaw and abdomen, multiple epidermal deprivation, and severe upper body subcutaneous deformities. unlike conventional vehicle tire punctures, dump truck tire rupture requires field investigation and examination in accordance with explosive damage. Active promotion and policies should be strengthened to prevent safety accidents related to tire rupture.

Cellular system에서의 깻잎의 ONOO⁻에 의한 산화적 스트레스 개선 및 항노화 효과

김현영(Hyun Young Kim),황보라(Bo Ra Hwang),Ting Ting Wu,조은주(Eun Ju Cho) 충남대학교 농업과학연구소 2012 농업과학연구 Vol.39 No.4

In this study, we investigated the antioxidative and antiaging activity of Perilla frutescens using LLC-PK₁ porcine renal epithelial cell and WI-38 human diploid fibroblast cell. The extract from Perilla frutescens showed strong protective effect against nitric oxide (NO) and superoxide (O₂?)-induced oxidative stress generated by sodium nitroprusside (SNP) and pyrogallol, respectively. The result showed that P. frutescens increased the cell viability and showed scavenging activity of NO and O₂?. In addition, the extract of P. frutescens exerted the protective effect against peroxynitrite (ONOO-) induced by 3-morpholinosydnonimine. It suggests that P. frutescens would have the protective role against ONOO? itself and its precursors, NO and O₂?. Furthermore, the aging model of hydrogen peroxide (H₂O₂)-treated WI-38 human diploid fibroblast was employed to investigate the anti-aging effect of P. frutescens. H₂O₂-treated WI-38 cells showed the loss of cell viability, however before-treatment with P. frutescens to WI-38 cells under premature senescence could delay the cellular aging process. The present study suggests the antioxidative and antiaging potential against free radical-induced oxidative damage of P. frutescens.

Westgard Multi-Rules의 효율적 적용과 조치사항의 개선

정흥수,오윤정,배진수,백진영,황보라,신용환,Jung, Heung Soo,Oh, Youn Jung,Bae, Jin Soo,Baek, Jin Young,Hwang, Bo ra,Shin, Yong Hwan 대한핵의학기술학회 2017 핵의학 기술 Vol.21 No.1

검사의 질 향상과 국제표준화의 상용화 정도관리물질을 이용한 Westgard multi-rules 적용의 유용성은 이미 알려져 있다. 그러나 핵의학 체외검사의 특성상 정도관리물질과 환자검체의 동시 계측으로 인한 측정횟수의 증가에 따라 Westgard multi-rules법을 적용함에 있어 어려움이 있다. 이에 본 연구는 핵의학 체외검사에서 상용화 정도관리물질을 이용한 Westgard multi-rules 적용의 유용성과 보완, 개선을 통해 내부정도관리의 효율성 향상을 조사하였다. 2013년 01월부터 2016년 06월까지 삼성서울병원 핵의학과 체외검사실 통합의료시스템에 기록된 총 282건의 적용된 계통오차 multi-rules (22s, 101s)과 117건의 조치사항 기록을 분석하였다. 조치사항은 multi-rules 중 계통오차의 규칙이 적용 되었을 때 기록하는 원인분석으로 정도관리물질 오류, 실험과정 오류, 검사키트 로트번호 관리 오류, 기타 등 총 4개의 대분류로 구성하였다. Westgard multi-rules 적용을 통해 조치사항을 분석한 결과 정도관리물질 오류가 62건, 실험과정 오류가 24건, 검사키트 로트번호 관리오류가 18건, 기타 13건으로 분류되었다. 정도관리물질 오류를 방지하고자 개선사항으로 기존에 각 검사자마다 사용하던 방식을 담당자 지정 방식으로 변경하여 모든 검사의 하루 소비량을 분주하여 공동사용을 하였고, 나머지 오류를 방지하고자 검사 전후 모든 과정을 표준화 하여 검사실내 어느 검사자가 시행 하더라도 일원화할 수 있게 하였다. 정도관리물질 오류를 개선한 결과 해동 후 2일 이내 신선한 물질을 사용 가능하였고 같은 물질을 사용하는 검사끼리 비교가 가능해져 물질에 의한 오류인지 명확해짐으로 계통오차 발생원인이 정도관리물질 오류로 기록하는 건수가 줄어들었다. 또한 정도관리물질의 로트번호 변경 시 교체시기가 같아 관리가 용이해졌고, 물질 사용량의 감소로 경제적 효과를 얻을 수 있었다. 그리고 검사표준화 적용 후, 계통오차의 규칙인 22s와 101s의 발생건수가 개선 전 보다 월 평균 2건 이상 줄어드는 결과를 보였다. Multi-rules의 적용을 통한 계통오차의 빠른 확인을 위해 정도관리물질의 체계적인 관리와 목표값과 표준편자의 설정 및 관리가 바탕이 되어야하며, 계통오차 발생 시 검사의 원인분석을 통한 조치사항을 기록하는 것이 중요함을 확인하였다. 본 실험의 결과로 Westgard multi-rules 적용 분석을 통해 발생 오류의 기재와 원인을 효율적으로 분석함으로써 핵의학 검사 내부정도관리의 질적 향상과 정확하고 신속한 결과보고에 기여할 것으로 사료된다. Purpose Westgard multi-rules application based on test quality improvement and commercialized international standard has been widely used in quality control. However, it is difficult to applicate the Westgard multi-rules in nuclear medicine in vitro tests due to the larger sample sizes and the simultaneous measurement of quality control material and patient sample. This study investigated the usefulness of Westgard multi-rules application in nuclear medicine in vitro tests. Materials and Methods A total of 282 systematic error multi-rules (22s, 101s) recorded in the samsung medical center computer system from January 2013 to June 2016 along with 117 cases of corrective measure record was analyzed. The Quality control implementation is recorded in Hospital information system were divided into 4 high-level areas including quality control material error, experimental procedural error, Kit lot number management error, and others. To prevent quality control material error, the existing method that each staff used their own method was changed. The staff who in charge of managing the quality control material was designated and daily consumption amount of every test was strictly controlled by one person. To prevent other errors, every test step was standardized so that the entire test procedures are identically implemented. Results The total quality control implementation was 117 cases; As a result, 62 quality control material errors were 62 cases, experimental process errors were 24 cases, Kit lot number control errors were 18 cases, and other errors were 13 cases. The quality control material error was corrected and could be used fresh materials within 2 days after thawing. The cases of systemic error were decreased to causes as quality control material error. The quality control materials were reduced above 10 vials to a monthly average. In addition, these errors of experimental processing and Kit lot number were improved by test standardization. Consequently, the cases of 101s and 22s in systematic error rules decreased at least 2 cases to a monthly average. Conclusion To confirm of systematic error through multi-rules application quickly, it is necessary to base on management of the QC material, target values and standard deviation. Moreover, in the event of a systematic error, it was found important to record measures based on test cause analysis. The experiment results are expected to contribute to internal quality control improvement and prompt and accurate result reporting through error recording and causal analysis based on Westgard multi-rules analysis.

보문 : 경기도 지역에 거주하는 일반인의 장례메이크업에 대한 인식

송수미 ( Su Mi Song ),황보라 ( Bo Ra Hwang ),차호연 ( Ho Yeon Cha ),김기영 ( Ki Young Kim ) 대한미용과학회(구 한국두피모발미용학회) 2010 대한미용학회지 Vol.6 No.2

With rapid economic growth, increasing interest in social welfare, and development of sciences, human life span is increasingly lengthened, leading to the creation of ageing society, where preparation for death-which cannot be overlooked-exercises great influence on the culture of funeral. Also, “funeral makeup”, which has developed due to social needs, is recently used by certain classes of people, and has yet to be furthered in order to either be recognized as a professional job or classified as such. Common people were surveyed to identify their perception of funeral makeup. In the result, more women than men said that a funeral makeup is necessary or that they will use funeral makeup. More people in their 20s and 30s, than people in their 50s, knew about funeral makeup. Regardless of age, education and religion, a large number of people said that a funeral makeup is necessary, and that they will use it. Considering these results, a greater number of those who knew about funeral makeup, and that a funeral makeup is necessary, responded that will use funeral makeup. Regardless of their majors, all respondents said that a funeral makeup is necessary, and regarding the preference of makeup performers as well, funeral makeup artists were preferred the most. Thus, most of people consider funeral makeup necessary, prefer the job of funeral makeup artists, indicating the need to provide professional guidance and education on funeral makeup, and if the service is widely promoted, the funeral makeup will emerge a new job among the makeup industry.

도시 텃밭 면적에 다른 계절별 작물의 생육조사

손명종(MyeongJong Son),김미진(MiJin Kim),황보라(BoRa Hwang),박수민(SuMin Park),허북구(BukGu Heo),박윤점(YunJum Park) 한국원예학회 2014 한국원예학회 학술발표요지 Vol.2014 No.5

에탄올과 과산화수소 처리 농도와 시간이 보존화 (Preserved Flower)용 장미의 탈색에 미치는 영향

박윤점(Yun-jum Park),김미진(Mi-jin Kim),황보라(Bo-ra Hwang),허북구(Buk-gu Heo) 한국원예학회 2014 한국원예학회 학술발표요지 Vol.2014 No.10

에어브러쉬를 이용한 색상, 구성, 질감의 표현기법

송원길(Won Kil Song),이현숙(Hyun Sook Lee),황보라(Bo Ra Hwang),차호연(Ho Yeon Cha),김기영(Ki Young Kim) 한국인체미용예술학회 2010 한국인체미용예술학회지 Vol.11 No.4

Air brush is now not only in car painting but also in body- and nail-painting. So that, practical expression techniques of color, construction and texture in body painting and nail art were investigated by air brush. 6 Pieces of art work are prepared on mannequin and synthetic nail for expression of multi- and mono-color, pictorial technique (free-hand), abstractive motif presented stencils, visual textual and the tactual textual by air brush. In the result, first, airbrush can be used not only the expression of animal and leaves detail image with multi- or mono-color, but also mono color with smooth gradation can be expressed the image perfectly. Second, the feeling with existing picture of Marilyn Munroe and abstractive motif can be expressed by using of pictorial technique and graphic technique with prepared stencils. And separately presented picture was very detail and fine painted, but a assembly picture showed unbalance. Because the area of expressed work may be small and narrow. Third, the visual textual and the tactual textual were expressed and felt truly and realistically. In the conclusion, air brush may be useful tool for coloring, construction and texture expression technique in body painting and nail art.